>VSE444 (VSE444Q) /CSM/VS/VSE4-B/VSE444Q.Seq.d/
AAAAAACTATATATAAAAAATGTCTTCAGGTATTGCTTTAGCTCCAAACTGCGTTTCAAC
CTTCAACGATTTAAAGTTAGGTCGTAAATATGGTGGTATCATCTACAGAATCTCTGACGA
TTCAAAAGAAATCATTGTTGACTCCACCTTACCAGCTGGTTGTTCATTCGATGAATTCAC
CAAATGTCTCCCAGAAAACGAATGTAGATACGTTGTCCTCGACTATCAATACAAAGAAGA
AGGTGCCCAAAAGAGCAAAATCTGTTTTGTTGCCTGGTGCCCAGATACCGCCAACATTAA
AAAGAAAATGATGGCCACCTCATCAAAAGACTCTTTACGTAAAGCCTGTGTTGGTATCCA
AGTTGAAATCCAAGGTACCGATGCCTCTGAAGTCAAAGATTCATGTTTCTATGAAAAATG
TACCAAAATCTAAATAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAA

knyi*KMSSGIALAPNCVSTFNDLKLGRKYGGIIYRISDDSKEIIVDSTLPAGCSFDEFT
KCLPENECRYVVLDYQYKEEGAQKSKICFVAWCPDTANIKKKMMATSSKDSLRKACVGIQ
VEIQGTDASEVKDSCFYEKCTKI*iikkkkkkkkkkkkkkk

Frame A:
kklyiknvfrycfssklrfnlqrfkvrs*iwwyhlqnl*rfkrnhc*lhltswlfir*ih
qmsprkrm*ircprlsiqrrrcpkeqnlfcclvpryrqh*kendghlikrlft*slcwyp
s*npryrcl*sqrfmfl*kmyqnlnnkkkkkkkkkkkkkkk


Frame B:
knyi*KMSSGIALAPNCVSTFNDLKLGRKYGGIIYRISDDSKEIIVDSTLPAGCSFDEFT
KCLPENECRYVVLDYQYKEEGAQKSKICFVAWCPDTANIKKKMMATSSKDSLRKACVGIQ
VEIQGTDASEVKDSCFYEKCTKI*iikkkkkkkkkkkkkkk


Frame C:
ktiykkclqvll*lqtafqpsti*s*vvnmvvsstesltiqkkslltppyqlvvhsmnsp
nvsqktnvdtlsstintkkkvpkraksvllpgaqipptlkrk*wpphqktlyvkpvlvsk
lkskvpmplkskihvsmknvpksk**kkkkkkkkkkkkkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ890 (VSJ890Q) /CSM/VS/VSJ8-D/VSJ890Q.Seq.d/                        868   0.0
VSJ584 (VSJ584Q) /CSM/VS/VSJ5-D/VSJ584Q.Seq.d/                        868   0.0
VSJ550 (VSJ550Q) /CSM/VS/VSJ5-C/VSJ550Q.Seq.d/                        868   0.0
VSJ437 (VSJ437Q) /CSM/VS/VSJ4-B/VSJ437Q.Seq.d/                        868   0.0
VSJ243 (VSJ243Q) /CSM/VS/VSJ2-B/VSJ243Q.Seq.d/                        868   0.0
VSG665 (VSG665Q) /CSM/VS/VSG6-C/VSG665Q.Seq.d/                        868   0.0
VSE444 (VSE444Q) /CSM/VS/VSE4-B/VSE444Q.Seq.d/                        868   0.0
VSD383 (VSD383Q) /CSM/VS/VSD3-D/VSD383Q.Seq.d/                        868   0.0
VFL333 (VFL333Q) /CSM/VF/VFL3-B/VFL333Q.Seq.d/                        868   0.0
VFE845 (VFE845Q) /CSM/VF/VFE8-B/VFE845Q.Seq.d/                        868   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

D37981|D37981.1 Dictyostelium discoidium DDCOF2 gene for cofilin, complete cds.   868  0.0  1
D37980|D37980.1 Dictyostelium discoidium DDCOF1 gene for cofilin, complete cds (exon1-2).   827  0.0  2
X04004|X04004.1 Dictyostelium discoideum mRNA for contact site A (csA) protein.   168  4e-38  1
CG272461|CG272461.1 OGWAU35TV ZM_0.7_1.5_KB Zea mays genomic clone ZMMBMa0524E21, genomic survey sequence.    34  0.33  3
BH330582|BH330582.1 CH230-94G12.TJC CHORI-230 Segment 1 Rattus norvegicus genomic clone CH230-94G12, DNA sequence.    30  0.96  3
AC060770|AC060770.3 Homo sapiens chromosome 1 clone RP11-185E9 map 1, WORKING DRAFT SEQUENCE, 20 unordered pieces.    34  1.4  5
AC135132|AC135132.2 Rattus norvegicus clone CH230-240G18, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    44  1.6  1
CB579150|CB579150.1 AMGNNUC:NRHY7-00010-A11-A nrhy7 (10850) Rattus norvegicus cDNA clone nrhy7-00010-a11 5', mRNA sequence.    44  1.6  1
BZ859527|BZ859527.1 CH240_269E4.TJ CHORI-240 Bos taurus genomic clone CH240_269E4, DNA sequence.    44  1.6  1
CB727158|CB727158.1 AMGNNUC:NRHY7-00026-B3-A nrhy7 (10850) Rattus norvegicus cDNA clone nrhy7-00026-b3 5', mRNA sequence.    44  1.6  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54706) RecName: Full=Cofilin-1;  &D37980_1(D37980|pid:none) &D...   280   1e-74
(P37167) RecName: Full=Actophorin;  &M93361_1(M93361|pid:none)        130   2e-29
FM992692_38(FM992692|pid:none) Candida dubliniensis CD36 chromos...   125   5e-28
(Q96VU9) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...   125   7e-28
AY428595_1(AY428595|pid:none) Helianthus annuus clone HA10-7 put...   123   2e-27
CP000501_299(CP000501|pid:none) Pichia stipitis CBS 6054 chromos...   120   2e-26
(Q9HF97) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...   119   5e-26
(Q6CQ22) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...   119   5e-26
CU928179_306(CU928179|pid:none) Zygosaccharomyces rouxii strain ...   116   2e-25
(Q03048) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...   116   2e-25

 psg: 0.91  gvh: 0.46  alm: 0.50  top: 0.60  tms: 0.00  mit: 0.30  mip: 0.06
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for VSE444 is nuc

>VSE444E.Seq
AAAAAACTATATATAAAAAATGTCTTCAGGTATTGCTTTAGCTCCAAACTGCGTTTCAAC
CTTCAACGATTTAAAGTTAGGTCGTAAATATGGTGGTATCATCTACAGAATCTCTGACGA
TTCAAAAGAAATCATTGTTGACTCCACCTTACCAGCTGGTTGTTCATTCGATGAATTCAC
CAAATGTCTCCCAGAAAACGAATGTAGATACGTTGTCCTCGACTATCAATACAAAGAAGA
AGGTGCCCAAAAGAGCAAAATCTGTTTTGTTGCCTGGTGCCCAGATACCGCCAACATTAA
AAAGAAAATGATGGCCACCTCATCAAAAGACTCTTTACGTAAAGCCTGTGTTGGTATCCA
AGTTGAAATCCAAGGTACCGATGCCTCTGAAGTCAAAGATTCATGTTTCTATGAAAAATG
TACCAAAATCTAAATAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAA