>VSE309 (VSE309Q) /CSM/VS/VSE3-A/VSE309Q.Seq.d/
CACATATTAAAATGCCAACCAGATTCAGTAAGCATAGAAAATCAAGAGGTGACGTTTGTG
CCGGTTACGGTCGTGTTGGTAAACACAGAAAGCATCCAGGTGGTCGTGGTAATGCTGGTG
GTCTCACTCATCACAGAATTAACTTTGACAAATACCATCCAGGTTATTTCGGTAAATTAG
GTATGAGACATTTCCATTTATTAAGAAACCAATACCATTGTCCAACCGTCTCATTAGAAA
AGATCTGGACTTTAGTCCCAGAATCCGTCAGAAAATCATTAGCTGCCAAGAATGACGGTA
CTGCTCCAGTTGTTGATGTCACCCAAAAAGGTTTCTTCAAAGTTTTAGGTCACGGTATCC
TCCCAACTCAACCAATCATCGTCAAAGCTCGTTATTTCTCTAAAGTCGCTGAAAAGAAAA
TCAAAGCTGTCGGTGGTGCTTGTATCTTAGTTGCTTAAATATTTTATACGAATCAATAAA
TATAAATAAAAAAAGTAATTAAATTTAAAAAATATAAAAAGAAAA

HIKMPTRFSKHRKSRGDVCAGYGRVGKHRKHPGGRGNAGGLTHHRINFDKYHPGYFGKLG
MRHFHLLRNQYHCPTVSLEKIWTLVPESVRKSLAAKNDGTAPVVDVTQKGFFKVLGHGIL
PTQPIIVKARYFSKVAEKKIKAVGGACILVA*ifytnq*i*ikkvikfkkykkk

Frame A:
hilkcqpdsvsienqevtfvpvtvvlvntesiqvvvvmlvvsliteltltntiqvisvn*
v*disiy*etntivqpsh*krsgl*sqnpsenh*lprmtvllqllmspkkvsskf*vtvs
sqlnqsssklvislkslkrksklsvvlvs*llkyfirinkyk*kk*lnlknikrk


Frame B:
ty*nanqiq*a*kikr*rlcrlrscw*tqkasrwsw*cwwshssqn*l*qipsrlfr*ir
yetfpfikkpiplsnrlirkdldfsprirqkiiscqe*rycssc*chpkrflqsfrsryp
pnstnhrqsslfl*sr*kenqscrwclylsclnilyesininkksn*i*ki*ke


Frame C:
HIKMPTRFSKHRKSRGDVCAGYGRVGKHRKHPGGRGNAGGLTHHRINFDKYHPGYFGKLG
MRHFHLLRNQYHCPTVSLEKIWTLVPESVRKSLAAKNDGTAPVVDVTQKGFFKVLGHGIL
PTQPIIVKARYFSKVAEKKIKAVGGACILVA*ifytnq*i*ikkvikfkkykkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ737 (VSJ737Q) /CSM/VS/VSJ7-B/VSJ737Q.Seq.d/                        940   0.0
VSI571 (VSI571Q) /CSM/VS/VSI5-C/VSI571Q.Seq.d/                        940   0.0
VSH826 (VSH826Q) /CSM/VS/VSH8-B/VSH826Q.Seq.d/                        940   0.0
VSF775 (VSF775Q) /CSM/VS/VSF7-D/VSF775Q.Seq.d/                        940   0.0
VSE523 (VSE523Q) /CSM/VS/VSE5-A/VSE523Q.Seq.d/                        940   0.0
VSE358 (VSE358Q) /CSM/VS/VSE3-C/VSE358Q.Seq.d/                        940   0.0
VSE309 (VSE309Q) /CSM/VS/VSE3-A/VSE309Q.Seq.d/                        940   0.0
VSE196 (VSE196Q) /CSM/VS/VSE1-D/VSE196Q.Seq.d/                        940   0.0
VSD784 (VSD784Q) /CSM/VS/VSD7-D/VSD784Q.Seq.d/                        940   0.0
VSA744 (VSA744Q) /CSM/VS/VSA7-B/VSA744Q.Seq.d/                        940   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

D50338|D50338.1 Dictyostelium discoideum DNA for ribosomal protein, complete cds.   914   0.0    1
BI863538|BI863538.1 kx45d11.y1 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:RL2A_OSCBR O01358 60S RIBOSOMAL PROTEIN L27A ;, mRNA sequence.    82   2e-26  3
BP014972|BP014972.1 Ciona intestinalis cDNA, clone:ciad63m07, 5' end, single read.    72   5e-25  4
BP010592|BP010592.1 Ciona intestinalis cDNA, clone:ciad74n20, 5' end, single read.    72   2e-24  4
BW126894|BW126894.1 Ciona intestinalis cDNA, clone:rcign012m06, 3' end, single read.    72   2e-24  4
BW094561|BW094561.1 Ciona intestinalis cDNA, clone:rcitb049o03, 3' end, single read.    72   2e-24  4
BW124755|BW124755.1 Ciona intestinalis cDNA, clone:rciad100o10, 3' end, single read.    72   2e-24  4
BM961080|BM961080.1 cihA11A16T Ascidian hemocytes cDNA library Ciona intestinalis cDNA, mRNA sequence.    72   2e-24  4
BP005921|BP005921.1 Ciona intestinalis cDNA, clone:ciad39g13, 5' end, single read.    72   2e-24  4
AV986190|AV986190.1 Ciona intestinalis cDNA, clone:cilv41c03, 5' end, single read.    72   2e-24  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P48160) RecName: Full=60S ribosomal protein L27a;  &D50338_1(D5...   313   2e-84
BT046766_1(BT046766|pid:none) Salmo salar clone ssal-eve-548-138...   226   2e-58
AY961477_1(AY961477|pid:none) Phytophthora infestans clone MY-19...   226   3e-58
AF502247_1(AF502247|pid:none) Epinephelus coioides ribosomal pro...   223   3e-57
AJ843085_1(AJ843085|pid:none) Platichthys flesus mRNA for 60S ri...   223   3e-57
(P18445) RecName: Full=60S ribosomal protein L27a;  &R5RTLA(S130...   222   4e-57
AB374966_1(AB374966|pid:none) Solea senegalensis RPL27a mRNA for...   221   7e-57
(P46776) RecName: Full=60S ribosomal protein L27a;  &(Q5REY2) Re...   221   7e-57
AJ307670_3(AJ307670|pid:none) Mus musculus St5 gene, L27a gene a...   221   7e-57
AB172987_1(AB172987|pid:none) Macaca fascicularis brain cDNA clo...   220   1e-56

 psg: 0.33  gvh: 0.18  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.44  mip: 0.12
 nuc: 0.05  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.50  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 20.0 %: mitochondrial
	 20.0 %: nuclear
	  4.0 %: plasma membrane

>> prediction for VSE309 is cyt

>VSE309E.Seq
CACATATTAAAATGCCAACCAGATTCAGTAAGCATAGAAAATCAAGAGGTGACGTTTGTG
CCGGTTACGGTCGTGTTGGTAAACACAGAAAGCATCCAGGTGGTCGTGGTAATGCTGGTG
GTCTCACTCATCACAGAATTAACTTTGACAAATACCATCCAGGTTATTTCGGTAAATTAG
GTATGAGACATTTCCATTTATTAAGAAACCAATACCATTGTCCAACCGTCTCATTAGAAA
AGATCTGGACTTTAGTCCCAGAATCCGTCAGAAAATCATTAGCTGCCAAGAATGACGGTA
CTGCTCCAGTTGTTGATGTCACCCAAAAAGGTTTCTTCAAAGTTTTAGGTCACGGTATCC
TCCCAACTCAACCAATCATCGTCAAAGCTCGTTATTTCTCTAAAGTCGCTGAAAAGAAAA
TCAAAGCTGTCGGTGGTGCTTGTATCTTAGTTGCTTAAATATTTTATACGAATCAATAAA
TATAAATAAAAAAAGTAATTAAATTTAAAAAATATAAAAAGAAAA