>VSE171 (VSE171Q) /CSM/VS/VSE1-C/VSE171Q.Seq.d/
XXXXXXXXXXGTATTGCTTTAGCTCCAAACTGCGTTTCAACCTTCAACGATTTAAAGTTA
GGTCGTAAATATGGTGGTATCATCTACAGAATCTCTGACGATTCAAAAGAAATCATTGTT
GACTCCACCTTACCAGCTGGTTGTTCATTCGATGAATTCACCAAATGTCTCCCAGAAAAC
GAATGTAGATACGTTGTCCTCGACTATCAATACAAAGAAGAAGGTGCCCAAAAGNGCAAA
ATCTGTTTTGTTGCCTGGTGCCCAGATACCGCCAACATTAAAAAGAAAATGATGGCCACC
TCATCAAAAGACTCTTTACGTAAAGCCTGTGTTGGTATCCAAGTTGAAATCCAAGGTACC
GATGCCTCTGAAGTCAAAGATTCATGTTTCTATGAAAAATGTACCAAAATCTAAATAATT
TGAAAAAATTTAAAAAAAAAAAAAAAAAAAAAACTCCCGAGGAACGAATTCAATATTTAT
AAATAGTAATAAATCTCCTCATACATATAATTTAATAAAATAATCTGTATTTTCTAAAAA
A

---IALAPNCVSTFNDLKLGRKYGGIIYRISDDSKEIIVDSTLPAGCSFDEFTKCLPENE
CRYVVLDYQYKEEGAQKXKICFVAWCPDTANIKKKMMATSSKDSLRKACVGIQVEIQGTD
ASEVKDSCFYEKCTKI*ii*knlkkkkkkklprnefniyk***issyi*fnkiicif*k

Frame A:
---ycfssklrfnlqrfkvrs*iwwyhlqnl*rfkrnhc*lhltswlfir*ihqmsprkr
m*ircprlsiqrrrcpkxqnlfcclvpryrqh*kendghlikrlft*slcwyps*npryr
cl*sqrfmfl*kmyqnlnnlkkfkkkkkkktpeeriqyl*ivinllihii**nnlyflk


Frame B:
---IALAPNCVSTFNDLKLGRKYGGIIYRISDDSKEIIVDSTLPAGCSFDEFTKCLPENE
CRYVVLDYQYKEEGAQKXKICFVAWCPDTANIKKKMMATSSKDSLRKACVGIQVEIQGTD
ASEVKDSCFYEKCTKI*ii*knlkkkkkkklprnefniyk***issyi*fnkiicif*k


Frame C:
---ll*lqtafqpsti*s*vvnmvvsstesltiqkkslltppyqlvvhsmnspnvsqktn
vdtlsstintkkkvpkxaksvllpgaqipptlkrk*wpphqktlyvkpvlvsklkskvpm
plkskihvsmknvpksk*feki*kkkkkknsrgtnsifinsnksphtynlik*svfskk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ890 (VSJ890Q) /CSM/VS/VSJ8-D/VSJ890Q.Seq.d/                        829   0.0
VSJ584 (VSJ584Q) /CSM/VS/VSJ5-D/VSJ584Q.Seq.d/                        829   0.0
VSJ550 (VSJ550Q) /CSM/VS/VSJ5-C/VSJ550Q.Seq.d/                        829   0.0
VSJ437 (VSJ437Q) /CSM/VS/VSJ4-B/VSJ437Q.Seq.d/                        829   0.0
VSJ243 (VSJ243Q) /CSM/VS/VSJ2-B/VSJ243Q.Seq.d/                        829   0.0
VSI135 (VSI135Q) /CSM/VS/VSI1-B/VSI135Q.Seq.d/                        829   0.0
VSG665 (VSG665Q) /CSM/VS/VSG6-C/VSG665Q.Seq.d/                        829   0.0
VSE171 (VSE171Q) /CSM/VS/VSE1-C/VSE171Q.Seq.d/                        829   0.0
VSD383 (VSD383Q) /CSM/VS/VSD3-D/VSD383Q.Seq.d/                        829   0.0
VSD320 (VSD320Q) /CSM/VS/VSD3-A/VSD320Q.Seq.d/                        829   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

D37981|D37981.1 Dictyostelium discoidium DDCOF2 gene for cofilin, complete cds.   829  0.0  2
D37980|D37980.1 Dictyostelium discoidium DDCOF1 gene for cofilin, complete cds (exon1-2).   829  0.0  2
X04004|X04004.1 Dictyostelium discoideum mRNA for contact site A (csA) protein.   168  4e-38  1
AF366647|AF366647.1 Gaultheria strigosa maturase K (matK) gene, complete cds; chloroplast gene for chloroplast product.    44  0.051  2
AF366639|AF366639.1 Gaultheria megalodonta maturase K (matK) gene, complete cds; chloroplast gene for chloroplast product.    44  0.051  2
AF366632|AF366632.1 Gaultheria foliolosa maturase K (matK) gene, complete cds; chloroplast gene for chloroplast product.    44  0.051  2
AF366628|AF366628.1 Gaultheria appressa maturase K (matK) gene, complete cds; chloroplast gene for chloroplast product.    44  0.051  2
AL606471|AL606471.4 Human DNA sequence *** SEQUENCING CANCELLED *** from clone  RP11-279L1.    48  0.11  1
AL603914|AL603914.3 Human DNA sequence from clone RP11-569A2 on chromosome 6.    48  0.11  1
AC024830|AC024830.2 Caenorhabditis elegans cosmid Y55F3BR, complete sequence.    48  0.11  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54706) RecName: Full=Cofilin-1;  &D37980_1(D37980|pid:none) &D...   271   8e-72
(P37167) RecName: Full=Actophorin;  &M93361_1(M93361|pid:none)        125   5e-28
(Q96VU9) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...   120   2e-26
FM992692_38(FM992692|pid:none) Candida dubliniensis CD36 chromos...   119   4e-26
AY428595_1(AY428595|pid:none) Helianthus annuus clone HA10-7 put...   117   2e-25
PDBN(1CNU)A MOL_ID: 1;MOL_ID: 1; MOLECULE: ACTOPHORIN; MOLECULE:...   115   1e-24
CP000501_299(CP000501|pid:none) Pichia stipitis CBS 6054 chromos...   114   2e-24
(Q9HF97) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...   113   4e-24
(Q6CQ22) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...   113   4e-24
AY635996_1(AY635996|pid:none) Monacrosporium haptotylum cofilin-...   111   1e-23

 psg: 0.84  gvh: 0.46  alm: 0.50  top: 0.67  tms: 0.00  mit: 0.23  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for VSE171 is cyt

>VSE171Z.Seq
----------GTATTGCTTTAGCTCCAAACTGCGTTTCAACCTTCAACGATTTAAAGTTA
GGTCGTAAATATGGTGGTATCATCTACAGAATCTCTGACGATTCAAAAGAAATCATTGTT
GACTCCACCTTACCAGCTGGTTGTTCATTCGATGAATTCACCAAATGTCTCCCAGAAAAC
GAATGTAGATACGTTGTCCTCGACTATCAATACAAAGAAGAAGGTGCCCAAAAGNGCAAA
ATCTGTTTTGTTGCCTGGTGCCCAGATACCGCCAACATTAAAAAGAAAATGATGGCCACC
TCATCAAAAGACTCTTTACGTAAAGCCTGTGTTGGTATCCAAGTTGAAATCCAAGGTACC
GATGCCTCTGAAGTCAAAGATTCATGTTTCTATGAAAAATGTACCAAAATCTAAATAATT
TGAAAAAATTTAAAAAAAAAAAAAAAAAAAAAACTCCCGAGGAACGAATTCAATATTTAT
AAATAGTAATAAATCTCCTCATACATATAATTTAATAAAATAATCTGTATTTTCTAAAAA
A