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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSD854
Library VS
(Link to library)
Clone ID VSD854
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10799-1
Original site URL
Representative seq. ID VSD854P
(Link to Original site)
Representative DNA sequence 
>VSD854 (VSD854Q) /CSM/VS/VSD8-C/VSD854Q.Seq.d/
CCAAATGTTGTTAGTTTATTTGATGTACTTCATTGTCAAAATAGACTTTATTTGGTATTT
GAATATTTAGATCAAGATTTAAAGAAATATATGGATTCCGTACCAGCATTATGTCCACAA
TTGATAAAGAGTTACCTTTATCAATTATTGAAGGGTTTAGCATACAGTCATGGTCATAGA
ATTTTACATAGAGATTTGAAACCACAAAATCTTTTAATCGATCGTCAAGGTGCATTGAAA
TTGGCTGATTTTGGTTTAGCTCGTGCAGTTAGTATTCCAGTACGTGTTTACACTCACGAG
ATTGTAACCCTTTGGTATCGTGCACCAGAGGTCTTATTGGGTTCAAAAAGTTACTCTGTA
CCAGTCGATATGTGGTCTGTTGGTTGTATCTTTGGTGAAATGTTAAACAAAAAACCATTG
TTTAGTGGTGATTGTGAAATTGATCAAATCTTTAGAATCTTTAGAGTTTTAGGTACTCCA
GATGATTCAATTTGGCCAGGTGTCACTAAACTTCCAGAATATGTXXXXXXXXXXCGCCAA
ATGTTGTTAGTTTATTTGATGTACTTCATTGTCAAAATAGACTTTATTTGGTATTTGAAT
ATTTAGATCAAGATTTAAAGAAATATATGGATTCCGTACCAGCATTATGTCCACAATTGA
TAAAGAGTTACCTTTATCAATTATTGAAGGGTTTAGCATACAGTCATGGTCATAGAATTT
TACATAGAGATTTGAAACCACAAAATCTTTTAATCGATCGTCAAGGTGCATTGAAATTGG
CTGATTTTGGTTTAGCTCGTGCAGTTAGTATTCCAGTACGTGTTTACACTCACGAGATTG
TAACCCTTTGGTATCGTGCACCAGAGGTCTTATTGGGTTCAAAAAGTTACTCTGTACCAG
TCGATATGTGGTCTGTTGGTTGTATCTTTGGTGAAATGTTAAACAAAAAACCATTGTTTA
GTGGTGATTGTGAAATTGATCAAATCTTTAGAATCTTTAGAGTTTTAGGTACTCCAGATG
ATTCAATTTGGCCAGGTGTCACTAAACTTCCAGAATATGTTTCAACTTTCCCAAATTGGC
CAGGTCAACCATATAATAAAATTTTCCCAAGATGTGAACCACTTGCATTAGATTTAATTG
CTAAAATGCTTCAATATGAACCATCAAAGAGAATTTCAGCTAAAGAAGCACTTCTTCACC
CATATTTTG
sequence update 2001. 3.22
Translated Amino Acid sequence 
PNVVSLFDVLHCQNRLYLVFEYLDQDLKKYMDSVPALCPQLIKSYLYQLLKGLAYSHGHR
ILHRDLKPQNLLIDRQGALKLADFGLARAVSIPVRVYTHEIVTLWYRAPEVLLGSKSYSV
PVDMWSVGCIFGEMLNKKPLFSGDCEIDQIFRIFRVLGTPDDSIWPGVTKLPEY---

---PNVVSLFDVLHCQNRLYLVFEYLDQDLKKYMDSVPALCPQLIKSYLYQLLKGLAYSH
GHRILHRDLKPQNLLIDRQGALKLADFGLARAVSIPVRVYTHEIVTLWYRAPEVLLGSKS
YSVPVDMWSVGCIFGEMLNKKPLFSGDCEIDQIFRIFRVLGTPDDSIWPGVTKLPEYVST
FPNWPGQPYNKIFPRCEPLALDLIAKMLQYEPSKRISAKEALLHPYF
Translated Amino Acid sequence (All Frames) 
Frame A:
PNVVSLFDVLHCQNRLYLVFEYLDQDLKKYMDSVPALCPQLIKSYLYQLLKGLAYSHGHR
ILHRDLKPQNLLIDRQGALKLADFGLARAVSIPVRVYTHEIVTLWYRAPEVLLGSKSYSV
PVDMWSVGCIFGEMLNKKPLFSGDCEIDQIFRIFRVLGTPDDSIWPGVTKLPEY---

---rqmllvylmyfivkidfiwylni*iki*rniwipyqhyvhn**rvtfiny*rv*htv
mviefyiei*nhkif*sivkvh*nwlilv*lvqlvfqyvftltrl*pfgivhqrsywvqk
vtlyqsicgllvvslvkc*tknhclvvivkliksleslef*vlqmiqfgqvslnfqnmfq
lsqigqvnhiikfsqdvnhlh*i*llkcfnmnhqrefqlkkhffthil

Frame B:
qmllvylmyfivkidfiwylni*iki*rniwipyqhyvhn**rvtfiny*rv*htvmvie
fyiei*nhkif*sivkvh*nwlilv*lvqlvfqyvftltrl*pfgivhqrsywvqkvtly
qsicgllvvslvkc*tknhclvvivkliksleslef*vlqmiqfgqvslnfqnm---

---akcc*fi*ctslsk*tlfgi*ifrsrfkeiygfrtsimstidkelplsiiegfsiqs
ws*nft*rfettksfnrssrcieig*fwfsscs*ysstclhsrdcnplvsctrgligfkk
llctsryvvcwlylw*nvkqktiv*w*l*n*snl*nl*sfrysr*fnlarch*tsricfn
fpklarsti**nfpkm*ttcirfnc*nasi*tikenfs*rstsspif

Frame C:
kcc*fi*ctslsk*tlfgi*ifrsrfkeiygfrtsimstidkelplsiiegfsiqsws*n
ft*rfettksfnrssrcieig*fwfsscs*ysstclhsrdcnplvsctrgligfkkllct
sryvvcwlylw*nvkqktiv*w*l*n*snl*nl*sfrysr*fnlarch*tsric---

---PNVVSLFDVLHCQNRLYLVFEYLDQDLKKYMDSVPALCPQLIKSYLYQLLKGLAYSH
GHRILHRDLKPQNLLIDRQGALKLADFGLARAVSIPVRVYTHEIVTLWYRAPEVLLGSKS
YSVPVDMWSVGCIFGEMLNKKPLFSGDCEIDQIFRIFRVLGTPDDSIWPGVTKLPEYVST
FPNWPGQPYNKIFPRCEPLALDLIAKMLQYEPSKRISAKEALLHPYF
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSD854 (VSD854Q) /CSM/VS/VSD8-C/VSD854Q.Seq.d/                       2337   0.0
AFI530 (AFI530Q) /CSM/AF/AFI5-B/AFI530Q.Seq.d/                       1326   0.0
VFJ235 (VFJ235Q) /CSM/VF/VFJ2-B/VFJ235Q.Seq.d/                       1318   0.0
VFD805 (VFD805Q) /CSM/VF/VFD8-A/VFD805Q.Seq.d/                       1304   0.0
CHB353 (CHB353Q) /CSM/CH/CHB3-C/CHB353Q.Seq.d/                       1287   0.0
SFE390 (SFE390Q) /CSM/SF/SFE3-D/SFE390Q.Seq.d/                       1271   0.0
SHC839 (SHC839Q) /CSM/SH/SHC8-B/SHC839Q.Seq.d/                       1207   0.0
AFD356 (AFD356Q) /CSM/AF/AFD3-C/AFD356Q.Seq.d/                       1170   0.0
SHK251 (SHK251Q) /CSM/SH/SHK2-C/SHK251Q.Seq.d/                       1059   0.0
AFH221 (AFH221Q) /CSM/AF/AFH2-A/AFH221Q.Seq.d/                       1027   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

M80808|M80808.1 Dictyostelium discoideum p34-cdc2 protein (cdc2) mRNA, complete cds.  1334  0.0  1
AC117072|AC117072.2 Dictyostelium discoideum chromosome 2 map 3323568-3470138 strain AX4, complete sequence.  1181  0.0  7
L00652|L00652.1 Dictyostelium discoideum (crp) gene, complete cds.    80  4e-28  3
AX488906|AX488906.1 Sequence 6206 from Patent WO02053728.         54  4e-14  3
AR037044|AR037044.1 Sequence 3 from patent US 5801015.            54  4e-14  3
U40405|U40405.1 Candida albicans cdc2/CDC28/CDK1 homolog mRNA, complete cds.    54  4e-14  3
X80034|X80034.1 C.albicans CDC28 gene.                            54  6e-14  3
AB033276|AB033276.1 Candida albicans gene for negative regulator of PHO system CaPho85, complete cds.    50  2e-12  3
AF026545|AF026545.1 Pneumocystis carinii Cdc2 cyclin-dependent kinase (cdc2) mRNA, complete cds.    62  7e-12  3
AI998347|AI998347.1 701545340 A. thaliana, Columbia Col-0, rosette-2 Arabidopsis thaliana cDNA clone 701545340, mRNA sequence.    50  1e-10  3
dna update 2003. 8.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P34112) RecName: Full=Cell division control protein 2 homolog; ...   466   e-130
(P29618) RecName: Full=Cyclin-dependent kinase A-1;          Sho...   324   3e-87
FB667479_1(FB667479|pid:none) Sequence 1 from Patent WO2007141189.    322   2e-86
FB667509_1(FB667509|pid:none) Sequence 31 from Patent WO20071411...   320   6e-86
BT038008_1(BT038008|pid:none) Zea mays full-length cDNA clone ZM...   319   1e-85
EU969498_1(EU969498|pid:none) Zea mays clone 331095 cell divisio...   319   1e-85
AC113930_15(AC113930|pid:none) Oryza sativa (japonica cultivar-g...   319   1e-85
GM754310_1(GM754310|pid:none) Sequence 3 from Patent WO2008116829.    318   3e-85
EF081595_1(EF081595|pid:none) Picea sitchensis clone WS02710_M08...   318   3e-85
(P23111) RecName: Full=Cell division control protein 2 homolog; ...   317   5e-85
protein update 2009. 7.30
PSORT 

 psg: 0.75  gvh: 0.51  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  4.0 %: cytoskeletal

>> prediction for VSD854 is cyt
5' end seq. ID VSD854F
5' end seq. 
>VSD854F.Seq
CCAAATGTTGTTAGTTTATTTGATGTACTTCATTGTCAAAATAGACTTTATTTGGTATTT
GAATATTTAGATCAAGATTTAAAGAAATATATGGATTCCGTACCAGCATTATGTCCACAA
TTGATAAAGAGTTACCTTTATCAATTATTGAAGGGTTTAGCATACAGTCATGGTCATAGA
ATTTTACATAGAGATTTGAAACCACAAAATCTTTTAATCGATCGTCAAGGTGCATTGAAA
TTGGCTGATTTTGGTTTAGCTCGTGCAGTTAGTATTCCAGTACGTGTTTACACTCACGAG
ATTGTAACCCTTTGGTATCGTGCACCAGAGGTCTTATTGGGTTCAAAAAGTTACTCTGTA
CCAGTCGATATGTGGTCTGTTGGTTGTATCTTTGGTGAAATGTTAAACAAAAAACCATTG
TTTAGTGGTGATTGTGAAATTGATCAAATCTTTAGAATCTTTAGAGTTTTAGGTACTCCA
GATGATTCAATTTGGCCAGGTGTCACTAAACTTCCAGAATATGT----------
Length of 5' end seq. 524
3' end seq. ID VSD854Z
3' end seq. 
>VSD854Z.Seq
----------CGCCAAATGTTGTTAGTTTATTTGATGTACTTCATTGTCAAAATAGACTT
TATTTGGTATTTGAATATTTAGATCAAGATTTAAAGAAATATATGGATTCCGTACCAGCA
TTATGTCCACAATTGATAAAGAGTTACCTTTATCAATTATTGAAGGGTTTAGCATACAGT
CATGGTCATAGAATTTTACATAGAGATTTGAAACCACAAAATCTTTTAATCGATCGTCAA
GGTGCATTGAAATTGGCTGATTTTGGTTTAGCTCGTGCAGTTAGTATTCCAGTACGTGTT
TACACTCACGAGATTGTAACCCTTTGGTATCGTGCACCAGAGGTCTTATTGGGTTCAAAA
AGTTACTCTGTACCAGTCGATATGTGGTCTGTTGGTTGTATCTTTGGTGAAATGTTAAAC
AAAAAACCATTGTTTAGTGGTGATTGTGAAATTGATCAAATCTTTAGAATCTTTAGAGTT
TTAGGTACTCCAGATGATTCAATTTGGCCAGGTGTCACTAAACTTCCAGAATATGTTTCA
ACTTTCCCAAATTGGCCAGGTCAACCATATAATAAAATTTTCCCAAGATGTGAACCACTT
GCATTAGATTTAATTGCTAAAATGCTTCAATATGAACCATCAAAGAGAATTTCAGCTAAA
GAAGCACTTCTTCACCCATATTTTG
Length of 3' end seq. 675
Connected seq. ID VSD854P
Connected seq. 
>VSD854P.Seq
CCAAATGTTGTTAGTTTATTTGATGTACTTCATTGTCAAAATAGACTTTATTTGGTATTT
GAATATTTAGATCAAGATTTAAAGAAATATATGGATTCCGTACCAGCATTATGTCCACAA
TTGATAAAGAGTTACCTTTATCAATTATTGAAGGGTTTAGCATACAGTCATGGTCATAGA
ATTTTACATAGAGATTTGAAACCACAAAATCTTTTAATCGATCGTCAAGGTGCATTGAAA
TTGGCTGATTTTGGTTTAGCTCGTGCAGTTAGTATTCCAGTACGTGTTTACACTCACGAG
ATTGTAACCCTTTGGTATCGTGCACCAGAGGTCTTATTGGGTTCAAAAAGTTACTCTGTA
CCAGTCGATATGTGGTCTGTTGGTTGTATCTTTGGTGAAATGTTAAACAAAAAACCATTG
TTTAGTGGTGATTGTGAAATTGATCAAATCTTTAGAATCTTTAGAGTTTTAGGTACTCCA
GATGATTCAATTTGGCCAGGTGTCACTAAACTTCCAGAATATGT----------CGCCAA
ATGTTGTTAGTTTATTTGATGTACTTCATTGTCAAAATAGACTTTATTTGGTATTTGAAT
ATTTAGATCAAGATTTAAAGAAATATATGGATTCCGTACCAGCATTATGTCCACAATTGA
TAAAGAGTTACCTTTATCAATTATTGAAGGGTTTAGCATACAGTCATGGTCATAGAATTT
TACATAGAGATTTGAAACCACAAAATCTTTTAATCGATCGTCAAGGTGCATTGAAATTGG
CTGATTTTGGTTTAGCTCGTGCAGTTAGTATTCCAGTACGTGTTTACACTCACGAGATTG
TAACCCTTTGGTATCGTGCACCAGAGGTCTTATTGGGTTCAAAAAGTTACTCTGTACCAG
TCGATATGTGGTCTGTTGGTTGTATCTTTGGTGAAATGTTAAACAAAAAACCATTGTTTA
GTGGTGATTGTGAAATTGATCAAATCTTTAGAATCTTTAGAGTTTTAGGTACTCCAGATG
ATTCAATTTGGCCAGGTGTCACTAAACTTCCAGAATATGTTTCAACTTTCCCAAATTGGC
CAGGTCAACCATATAATAAAATTTTCCCAAGATGTGAACCACTTGCATTAGATTTAATTG
CTAAAATGCTTCAATATGAACCATCAAAGAGAATTTCAGCTAAAGAAGCACTTCTTCACC
CATATTTTG
Length of connected seq. 1199
Full length Seq ID -
Full length Seq. -
Length of full length seq. -