>VSD851 (VSD851Q) /CSM/VS/VSD8-C/VSD851Q.Seq.d/
CAGTTGGAAAAAACAAATAAATAAATTAAAATTTTTTTTTATAAAAAAAAAAXXXXXXXX
XXTGTTTTAGAATTAATTATATTGATTNTTCAAGTTCAACTTATGAATATGTCTATTTAG
GACCAACATTAAATTCAATTGAATTATTAGGAAATTCATTGAAATTTGATGCAGATGTTC
ATTGCCCAANTNCAGCTCCATCTTTAAGTATTATTATTAACTCTATCCCATTNTCAAGTA
ATATCCAAAATTCATTTGGTAATACTTATTTATATCAACCAACATTCACTCTTTGTACCG
ACTTTTCCAAATTATTTAATATTTCCATTACTGCCTCCTTTTCTTATTCTTCTTCAATTA
AGTTTACTTCAAATATGATTAAATTTGATTGTTCCATTGGTAAAGCTACTTCCTCTGATA
ATGGAACATCCACTCCTGTACCAACTTCTGAACCTTCCTCCACCCCAACTCAAACTCAAA
CTCAAACTCCAACTCCAACTCAAACTCAAACTCAAACTCCAACTCAAACTCATACACCAA
AACCAACAATCCCAAATGAAACAAGTGAATTTTATGATTCCTCTGCATCAAATCTCTCGA
TTACTAGCACTTTCTTTATTTTATT

SWKKQINKLKFFFIKKK---

---CFRINYIDXSSSTYEYVYLGPTLNSIELLGNSLKFDADVHCPXXAPSLSIIINSIPX
SSNIQNSFGNTYLYQPTFTLCTDFSKLFNISITASFSYSSSIKFTSNMIKFDCSIGKATS
SDNGTSTPVPTSEPSSTPTQTQTQTPTPTQTQTQTPTQTHTPKPTIPNETSEFYDSSASN
LSITSTFFIL

Frame A:
qlektnk*ikiffykkk---

---CFRINYIDXSSSTYEYVYLGPTLNSIELLGNSLKFDADVHCPXXAPSLSIIINSIPX
SSNIQNSFGNTYLYQPTFTLCTDFSKLFNISITASFSYSSSIKFTSNMIKFDCSIGKATS
SDNGTSTPVPTSEPSSTPTQTQTQTPTPTQTQTQTPTQTHTPKPTIPNETSEFYDSSASN
LSITSTFFIL

Frame B:
SWKKQINKLKFFFIKKK---

---vleliilixqvqlmnmsi*dqh*iqlny*eih*nlmqmfiaqxqlhl*vllltlshx
qviskihlviliyinqhslfvptfpnylifpllppflillqlsllqi*lnlivplvkllp
limehpllyqllnlpppqlklklklqlqlklklklqlklihqnqqsqmkqvnfmiplhqi
srllalslfy

Frame C:
vgknk*in*nffl*kk---

---f*n*ly*xfkfnl*iclfrtnikfn*iirkfiei*crcslpxxssifkyyy*lypix
k*ypkfiw*ylfistnihslyrlfqii*yfhycllflfffn*vyfkyd*i*lfhw*syfl
**wnihsctnf*tflhpnsnsnsnsnsnsnsnsnsnsnsytktnnpk*nk*il*flciks
ldy*hflyfi

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSD851 (VSD851Q) /CSM/VS/VSD8-C/VSD851Q.Seq.d/                        779   0.0
VFO866 (VFO866Q) /CSM/VF/VFO8-C/VFO866Q.Seq.d/                        779   0.0
VSJ140 (VSJ140Q) /CSM/VS/VSJ1-B/VSJ140Q.Seq.d/                        751   0.0
VSD677 (VSD677Q) /CSM/VS/VSD6-D/VSD677Q.Seq.d/                        613   e-174
VSG213 (VSG213Q) /CSM/VS/VSG2-A/VSG213Q.Seq.d/                        609   e-173
VSB355 (VSB355Q) /CSM/VS/VSB3-C/VSB355Q.Seq.d/                        575   e-163
VSF615 (VSF615Q) /CSM/VS/VSF6-A/VSF615Q.Seq.d/                        329   3e-89
VSG516 (VSG516Q) /CSM/VS/VSG5-A/VSG516Q.Seq.d/                        311   7e-84
VSJ489 (VSJ489Q) /CSM/VS/VSJ4-D/VSJ489Q.Seq.d/                        125   9e-28
VSI266 (VSI266Q) /CSM/VS/VSI2-C/VSI266Q.Seq.d/                        125   9e-28

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC133998|AC133998.2 Rattus norvegicus clone CH230-90P10, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    48  0.13  1
AC103064|AC103064.5 Rattus norvegicus clone CH230-169G11, *** SEQUENCING IN PROGRESS ***, 7 unordered pieces.    48  0.13  1
AC094032|AC094032.8 Rattus norvegicus clone CH230-96H3, WORKING DRAFT SEQUENCE, 2 unordered pieces.    48  0.13  1
AC146115|AC146115.1 Pan troglodytes chromosome UNK clone RP43-38M17, *** SEQUENCING IN PROGRESS ***, 9 unordered pieces.    36  0.37  3
AZ677796|AZ677796.1 ENTLR72TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    36  0.57  2
AC116330|AC116330.2 Dictyostelium discoideum chromosome 2 map 3191214-3323468 strain AX4, complete sequence.    40  0.57  4
AC107227|AC107227.5 Mus musculus clone RP23-62B24, *** SEQUENCING IN PROGRESS ***, 5 ordered pieces.    38  1.0  5
AC117176|AC117176.2 Dictyostelium discoideum chromosome 2 map 5018074-5200947 strain AX4, complete sequence.    38  1.4  6
CC474263|CC474263.1 CH240_299K9.TARBAC13P2 CHORI-240 Bos taurus genomic clone CH240_299K9, genomic survey sequence.    34  1.4  2
BX248326|BX248326.7 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone DKEY-260N20.    48  1.6  4

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3268448
Number of Hits to DB: 608,098,474

 psg: 0.65  gvh: 0.31  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.40  mip: 0.05
 nuc: 0.06  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: mitochondrial
	 28.0 %: nuclear
	 16.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VSD851 is mit

>VSD851F.Seq
CAGTTGGAAAAAACAAATAAATAAATTAAAATTTTTTTTTATAAAAAAAAAA--------
--

>VSD851Z.Seq
----------TGTTTTAGAATTAATTATATTGATTNTTCAAGTTCAACTTATGAATATGT
CTATTTAGGACCAACATTAAATTCAATTGAATTATTAGGAAATTCATTGAAATTTGATGC
AGATGTTCATTGCCCAANTNCAGCTCCATCTTTAAGTATTATTATTAACTCTATCCCATT
NTCAAGTAATATCCAAAATTCATTTGGTAATACTTATTTATATCAACCAACATTCACTCT
TTGTACCGACTTTTCCAAATTATTTAATATTTCCATTACTGCCTCCTTTTCTTATTCTTC
TTCAATTAAGTTTACTTCAAATATGATTAAATTTGATTGTTCCATTGGTAAAGCTACTTC
CTCTGATAATGGAACATCCACTCCTGTACCAACTTCTGAACCTTCCTCCACCCCAACTCA
AACTCAAACTCAAACTCCAACTCCAACTCAAACTCAAACTCAAACTCCAACTCAAACTCA
TACACCAAAACCAACAATCCCAAATGAAACAAGTGAATTTTATGATTCCTCTGCATCAAA
TCTCTCGATTACTAGCACTTTCTTTATTTTATT

>VSD851P.Seq
CAGTTGGAAAAAACAAATAAATAAATTAAAATTTTTTTTTATAAAAAAAAAA--------
--TGTTTTAGAATTAATTATATTGATTNTTCAAGTTCAACTTATGAATATGTCTATTTAG
GACCAACATTAAATTCAATTGAATTATTAGGAAATTCATTGAAATTTGATGCAGATGTTC
ATTGCCCAANTNCAGCTCCATCTTTAAGTATTATTATTAACTCTATCCCATTNTCAAGTA
ATATCCAAAATTCATTTGGTAATACTTATTTATATCAACCAACATTCACTCTTTGTACCG
ACTTTTCCAAATTATTTAATATTTCCATTACTGCCTCCTTTTCTTATTCTTCTTCAATTA
AGTTTACTTCAAATATGATTAAATTTGATTGTTCCATTGGTAAAGCTACTTCCTCTGATA
ATGGAACATCCACTCCTGTACCAACTTCTGAACCTTCCTCCACCCCAACTCAAACTCAAA
CTCAAACTCCAACTCCAACTCAAACTCAAACTCAAACTCCAACTCAAACTCATACACCAA
AACCAACAATCCCAAATGAAACAAGTGAATTTTATGATTCCTCTGCATCAAATCTCTCGA
TTACTAGCACTTTCTTTATTTTATT