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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSD821
Library VS
(Link to library)
Clone ID VSD821
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16543-1
Original site URL
Representative seq. ID VSD821P
(Link to Original site)
Representative DNA sequence 
>VSD821 (VSD821Q) /CSM/VS/VSD8-A/VSD821Q.Seq.d/
CAAATAAATAATTTATATTAAAAAATGTCCAGAGTAATTCATATTTCATCTAATGAAGAA
TTAGATAAACATTTGCAAGCTGAAAGATTAGTGATTGACTTTAGTGCTGCATGGTGTGGT
CCATGTAGAGCAATATCTCCGGTATTTGAAAAGCTTTCAAACGAATTTGTAACATTTACA
TTCGTTCATGTTGACATTGATAAACTAAGTGGTCATCCAATAGTAAAAGAAATTAGAAGT
GTTCCTACATTCTATTTTTACAGAAATGGAGCTAAAGTATCAGAATTCAGTGGTGCCAAT
GAAGCAACTCTAAGAAGTACTCTAGAAGCAAACAAATAATTTTTTGTTAAATTAATAATT
GTACAAAAAATGCAACAAATTAAATAAATAAAAAAAAAAAAATANTTCNCAAATTNTTTT
NTTTAATTTAAAAAAAAAAXXXXXXXXXXCCANGGGTCCGCAAATAAATAATTTANATTA
AAAAATGTCCANAGTAATTCATATTTCATNTAATGAAGAATTAGATAAACATTTGCAAGC
TGAAANATTAGTGATTGACTTTAGTGCTGCATGGTGTGGNCCATGTANAGCAANATCTCC
GGNATTTNAAAAGCTTTCAAACGAATTTGNAACATTTACATTCGTTCATGTTGACATTGA
TAAACTAAGTGGTCATCCAATAGTAAAANAAATTANAAGNGNTCCTACATTCTATTTTTA
CANAAATGGAGCTAAAGNATCAGAATTCAGTGGNGCCAATGAAGCAACT
sequence update 2001. 3.22
Translated Amino Acid sequence 
k*iiyikkcpe*fifhlmkn*inicklkd**ltlvlhgvvhveqylrylksfqtnl*hlh
sfmltlin*vviq**kklevflhsiftemelkyqnsvvpmkql*evl*kqtnnflln**l
ykkcnklnk*KKKNXSQIXXFNLKKK---

---XGPQINNLX*kmsxvihisxneeldkhlqaexlvidfsaawcgpcxaxspxfxklsn
efxtftfvhvdidklsghpivkxixxxptfyfyxngakxsefsganeat
Translated Amino Acid sequence (All Frames) 
Frame A:
qinnly*kmsrvihissneeldkhlqaerlvidfsaawcgpcraispvfeklsnefvtft
fvhvdidklsghpivkeirsvptfyfyrngakvsefsganeatlrstleank*ffvklii
vqkmqqik*ikkkkxfxnxfx*fkkk---

---pxvrk*iixikkcpx*fifhxmkn*inicklkx**ltlvlhgvxhvxqxlrxxksfq
tnlxhlhsfmltlin*vviq**xklxxxlhsiftxmelkxqnsvxpmkq

Frame B:
k*iiyikkcpe*fifhlmkn*inicklkd**ltlvlhgvvhveqylrylksfqtnl*hlh
sfmltlin*vviq**kklevflhsiftemelkyqnsvvpmkql*evl*kqtnnflln**l
ykkcnklnk*KKKNXSQIXXFNLKKK---

---xgsank*fxlknvxsnsyfx**rir*tfas*xisd*l*ccmvwxmxsxisgixkafk
rixniyirsc*h**tkwssnskxnxkxsyilflxkws*xiriqwxq*sn

Frame C:
nk*filknvqsnsyfi**rir*tfas*kisd*l*ccmvwsm*snisgi*kafkricniyi
rsc*h**tkwssnskrn*kcsyilflqkws*siriqwcq*snskkysrskqiifc*innc
tknatn*inkkkkixxkxfxli*kk---

---XGPQINNLX*kmsxvihisxneeldkhlqaexlvidfsaawcgpcxaxspxfxklsn
efxtftfvhvdidklsghpivkxixxxptfyfyxngakxsefsganeat
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ416 (VSJ416Q) /CSM/VS/VSJ4-A/VSJ416Q.Seq.d/                        763   0.0
VSI646 (VSI646Q) /CSM/VS/VSI6-B/VSI646Q.Seq.d/                        763   0.0
VSD821 (VSD821Q) /CSM/VS/VSD8-A/VSD821Q.Seq.d/                        763   0.0
VSD665 (VSD665Q) /CSM/VS/VSD6-C/VSD665Q.Seq.d/                        763   0.0
VSD645 (VSD645Q) /CSM/VS/VSD6-B/VSD645Q.Seq.d/                        763   0.0
SSM584 (SSM584Q) /CSM/SS/SSM5-D/SSM584Q.Seq.d/                        763   0.0
SSM341 (SSM341Q) /CSM/SS/SSM3-B/SSM341Q.Seq.d/                        763   0.0
SSE809 (SSE809Q) /CSM/SS/SSE8-A/SSE809Q.Seq.d/                        763   0.0
SSA493 (SSA493Q) /CSM/SS/SSA4-D/SSA493Q.Seq.d/                        763   0.0
AHG261 (AHG261Q) /CSM/AH/AHG2-C/AHG261Q.Seq.d/                        763   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU269880) Dictyostelium discoideum vegetative cDNA clone:VS...   650   0.0    1
(AU269018) Dictyostelium discoideum vegetative cDNA clone:VS...   650   0.0    1
(AU264605) Dictyostelium discoideum vegetative cDNA clone:VS...   650   0.0    1
(AU264394) Dictyostelium discoideum vegetative cDNA clone:VS...   650   0.0    1
(AU264360) Dictyostelium discoideum vegetative cDNA clone:VS...   650   0.0    1
(AU261936) Dictyostelium discoideum vegetative cDNA clone:VS...   650   0.0    1
(AU039068) Dictyostelium discoideum slug cDNA, clone SSM341.      650   0.0    1
(C93126) Dictyostelium discoideum slug cDNA, clone SSM584.        650   0.0    1
(BJ339191) Dictyostelium discoideum cDNA clone:dda64p09, 5' ...   650   0.0    1
(BJ333680) Dictyostelium discoideum cDNA clone:dda43i16, 5' ...   650   0.0    1
dna update 2009. 7.27
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P29447) RecName: Full=Thioredoxin-3;          Short=Trx-3;           185   2e-45
C46264(C46264) thioredoxin 3 - slime mold (Dictyostelium discoid...   182   1e-44
(P29446) RecName: Full=Thioredoxin-2;          Short=Trx-2; Flag...   182   1e-44
BC129794_1(BC129794|pid:none) Xenopus laevis hypothetical protei...    94   6e-18
AB478083_1(AB478083|pid:none) Pichia pastoris TRX2 gene for thio...    93   8e-18
BC166957_1(BC166957|pid:none) Xenopus tropicalis hypothetical pr...    92   1e-17
BC072884_1(BC072884|pid:none) Xenopus laevis MGC80314 protein, m...    91   4e-17
(Q9DGI3) RecName: Full=Thioredoxin;          Short=Trx;  &AF2936...    87   6e-16
FJ384985_1(FJ384985|pid:none) Bombina orientalis thioredoxin mRN...    85   2e-15
DQ215312_1(DQ215312|pid:none) Taeniopygia guttata clone 0058P004...    85   3e-15
protein update 2009. 7.30
PSORT 

 psg: 0.77  gvh: 0.17  alm: 0.69  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.00
 nuc: 0.25  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: nuclear
	 16.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: plasma membrane

>> prediction for VSD821 is nuc
5' end seq. ID VSD821F
5' end seq. 
>VSD821F.Seq
CAAATAAATAATTTATATTAAAAAATGTCCAGAGTAATTCATATTTCATCTAATGAAGAA
TTAGATAAACATTTGCAAGCTGAAAGATTAGTGATTGACTTTAGTGCTGCATGGTGTGGT
CCATGTAGAGCAATATCTCCGGTATTTGAAAAGCTTTCAAACGAATTTGTAACATTTACA
TTCGTTCATGTTGACATTGATAAACTAAGTGGTCATCCAATAGTAAAAGAAATTAGAAGT
GTTCCTACATTCTATTTTTACAGAAATGGAGCTAAAGTATCAGAATTCAGTGGTGCCAAT
GAAGCAACTCTAAGAAGTACTCTAGAAGCAAACAAATAATTTTTTGTTAAATTAATAATT
GTACAAAAAATGCAACAAATTAAATAAATAAAAAAAAAAAAATANTTCNCAAATTNTTTT
NTTTAATTTAAAAAAAAAA----------
Length of 5' end seq. 439
3' end seq. ID VSD821Z
3' end seq. 
>VSD821Z.Seq
----------CCANGGGTCCGCAAATAAATAATTTANATTAAAAAATGTCCANAGTAATT
CATATTTCATNTAATGAAGAATTAGATAAACATTTGCAAGCTGAAANATTAGTGATTGAC
TTTAGTGCTGCATGGTGTGGNCCATGTANAGCAANATCTCCGGNATTTNAAAAGCTTTCA
AACGAATTTGNAACATTTACATTCGTTCATGTTGACATTGATAAACTAAGTGGTCATCCA
ATAGTAAAANAAATTANAAGNGNTCCTACATTCTATTTTTACANAAATGGAGCTAAAGNA
TCAGAATTCAGTGGNGCCAATGAAGCAACT
Length of 3' end seq. 320
Connected seq. ID VSD821P
Connected seq. 
>VSD821P.Seq
CAAATAAATAATTTATATTAAAAAATGTCCAGAGTAATTCATATTTCATCTAATGAAGAA
TTAGATAAACATTTGCAAGCTGAAAGATTAGTGATTGACTTTAGTGCTGCATGGTGTGGT
CCATGTAGAGCAATATCTCCGGTATTTGAAAAGCTTTCAAACGAATTTGTAACATTTACA
TTCGTTCATGTTGACATTGATAAACTAAGTGGTCATCCAATAGTAAAAGAAATTAGAAGT
GTTCCTACATTCTATTTTTACAGAAATGGAGCTAAAGTATCAGAATTCAGTGGTGCCAAT
GAAGCAACTCTAAGAAGTACTCTAGAAGCAAACAAATAATTTTTTGTTAAATTAATAATT
GTACAAAAAATGCAACAAATTAAATAAATAAAAAAAAAAAAATANTTCNCAAATTNTTTT
NTTTAATTTAAAAAAAAAA----------CCANGGGTCCGCAAATAAATAATTTANATTA
AAAAATGTCCANAGTAATTCATATTTCATNTAATGAAGAATTAGATAAACATTTGCAAGC
TGAAANATTAGTGATTGACTTTAGTGCTGCATGGTGTGGNCCATGTANAGCAANATCTCC
GGNATTTNAAAAGCTTTCAAACGAATTTGNAACATTTACATTCGTTCATGTTGACATTGA
TAAACTAAGTGGTCATCCAATAGTAAAANAAATTANAAGNGNTCCTACATTCTATTTTTA
CANAAATGGAGCTAAAGNATCAGAATTCAGTGGNGCCAATGAAGCAACT
Length of connected seq. 759
Full length Seq ID -
Full length Seq. -
Length of full length seq. -