>VSD810 (VSD810Q) /CSM/VS/VSD8-A/VSD810Q.Seq.d/
AAACAATCAATTTAAAAAATGATGAAATTTTTAATTGTTTTAATTTTAGCATTATTCGCT
TTATCAAGTGTTAATGCAAGCAGCGCTATTGAATGCGAACTTTGTGAATATGCTGCCAAT
TATGGTGAATTGCTCATTCAATCAAATGCCACCGAAACTGAAATTATCGATAAAGTTGAA
AACTTTTGCAAAGTTATCCCATCTAGTTTCCAAGCCACTTGTGATAGTTTAATCGCAAAC
TATGGTAAACAACTCATTCAAATGATTGTCAACAAAGAATCACCAAGCACTTTATGCGCT
CAAATTGATATGTGTGCATCATCAACTGAAGCCGTCCAAGGTATTTTAGAATGTGACATT
TGCCAATTCATCGTTAAACAAGTCAACAAATATATTTCAGGTAACGCAACTGAAGCCCAA
ATCCTCAAATTCTTAGATACTGATTGTGAAGTCTTTGGTAAAGGTGGTTCAGTAACTTGT
CAAAACATTGTCAACANCTATGCCCCACAAATCATCAATTTAATCATTAACAACGCATCA
CCATCCCAAGTNTGCGGTCTTGTNGGTTTATGTGGCTCCTCTTTGAAAATCGAAGAACCA
GTCCAAGGTGAATTAGAATGTGGTGTTTGTGAAGTCATTGCTCAACAATGCTCAAACTAC
ATCAAAGCCAACAAANCCGAATCTGAAATCGTTGGTCTCCTCGATCAATTCTGTTCAACT
CTTTCAATCTTTGAAAGTGCTTGTGATACCATCGTTGCCAGCTCTGCTCCAAAGATCATC
AACTTACTCTTACAAAACCAAAGTGCAACTGTAGTTTGTACCGAAATTGGCTTCTGTGGT
AACTCTTCATCAGGTAATNACAACCATTCATCATCTGAAGAATCTTCATCGTCTTCCCAA
CCATCATCATCTTCTGAATCATCAGAATCAAGTTACTCNGGTTCAATGACTGGTGCTTCA
TCTGGTTCAGCTTCAGGTTCAGGTTCAGGTACATCNGAAACATGAAAATTTACATCAAAT
AAATTTAANAATTAAATAATTTA

kqsi*KMMKFLIVLILALFALSSVNASSAIECELCEYAANYGELLIQSNATETEIIDKVE
NFCKVIPSSFQATCDSLIANYGKQLIQMIVNKESPSTLCAQIDMCASSTEAVQGILECDI
CQFIVKQVNKYISGNATEAQILKFLDTDCEVFGKGGSVTCQNIVNXYAPQIINLIINNAS
PSQVCGLVGLCGSSLKIEEPVQGELECGVCEVIAQQCSNYIKANKXESEIVGLLDQFCST
LSIFESACDTIVASSAPKIINLLLQNQSATVVCTEIGFCGNSSSGNXNHSSSEESSSSSQ
PSSSSESSESSYSGSMTGASSGSASGSGSGTSET*kftsnkfxn*ii

Frame A:
kqsi*KMMKFLIVLILALFALSSVNASSAIECELCEYAANYGELLIQSNATETEIIDKVE
NFCKVIPSSFQATCDSLIANYGKQLIQMIVNKESPSTLCAQIDMCASSTEAVQGILECDI
CQFIVKQVNKYISGNATEAQILKFLDTDCEVFGKGGSVTCQNIVNXYAPQIINLIINNAS
PSQVCGLVGLCGSSLKIEEPVQGELECGVCEVIAQQCSNYIKANKXESEIVGLLDQFCST
LSIFESACDTIVASSAPKIINLLLQNQSATVVCTEIGFCGNSSSGNXNHSSSEESSSSSQ
PSSSSESSESSYSGSMTGASSGSASGSGSGTSET*kftsnkfxn*ii


Frame B:
nnqfkk**nf*lf*f*hyslyqvlmqaallnanfvnmlpimvncsfnqmppklklsiklk
tfaklshlvskplviv*sqtmvnnsfk*lstknhqalyalklicvhhqlkpskvf*nvtf
ansslnkstnifqvtqlkpkssns*ilivkslvkvvq*lvktlstxmphkssi*sltthh
hpkxavlxvyvapl*ksknqskvn*nvvfvksllnnaqttskptxpnlkslvssinsvql
fqslkvlvipslpallqrsstysyktkvql*fvpklasvvtlhqvxttihhlknlhrlpn
hhhllnhqnqvtxvq*lvlhlvqlqvqvqvhxkhenlhqinlxik*f


Frame C:
tinlkndeifncfnfsiirfikc*ckqry*mrtl*iccqlw*iahsikchrn*nyr*s*k
llqsypi*fpshl**fnrklw*tthsndcqqritkhfmrsn*yvciin*srpryfrm*hl
pihr*tsqqiyfr*rn*spnpqilry*l*slw*rwfsnlskhcqxlcptnhqfnh*qrit
ipsxrscxfmwllfenrrtspr*irmwcl*shcstmlklhqsqqxri*nrwsprsilfns
fnl*kcl*yhrcqlcskdhqltltkpkcncslyrnwllw*lfir*xqpfii*rifivfpt
iiif*iirikllxfndwcfiwfsfrfrfryixnmkiyik*i*xlnnl

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSD810 (VSD810Q) /CSM/VS/VSD8-A/VSD810Q.Seq.d/                       2020   0.0
VFL608 (VFL608Q) /CSM/VF/VFL6-A/VFL608Q.Seq.d/                       2006   0.0
FCL-AB23 (FCL-AB23Q) /CSM/FCL/FCL-AB/FCL-AB23Q.Seq.d/                1988   0.0
VFL571 (VFL571Q) /CSM/VF/VFL5-C/VFL571Q.Seq.d/                       1941   0.0
VSJ537 (VSJ537Q) /CSM/VS/VSJ5-B/VSJ537Q.Seq.d/                       1939   0.0
SLE371 (SLE371Q) /CSM/SL/SLE3-C/SLE371Q.Seq.d/                       1921   0.0
VSD442 (VSD442Q) /CSM/VS/VSD4-B/VSD442Q.Seq.d/                       1917   0.0
VSG309 (VSG309Q) /CSM/VS/VSG3-A/VSG309Q.Seq.d/                       1909   0.0
VFF843 (VFF843Q) /CSM/VF/VFF8-B/VFF843Q.Seq.d/                       1651   0.0
VFF888 (VFF888Q) /CSM/VF/VFF8-D/VFF888Q.Seq.d/                       1168   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AU206530|AU206530.1 Caenorhabditis elegans cDNA clone:yk877f01 : 5' end, single read.   230  3e-56  1
AF175768|AF175768.1 Dictyostelium discoideum SAPLIP C protein mRNA, complete cds.    84  3e-14  3
X52584|X52584.1 T. brucei PARP-A locus for PARP-A alpha and beta proteins.    52  0.014  1
M33129|M33129.1 T.brucei procyclic acidic repetitive protein A.alpha and A.beta (parp-A.alpha and parp-A.beta) genes, complete cds.    52  0.014  1
BX564787|BX564787.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse73f11_q1c.    52  0.014  1
BX558403|BX558403.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse38d05_q1c.    52  0.014  1
BX549701|BX549701.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse109d11_q1c.    52  0.014  1
AY223858|AY223858.1 Trypanosoma evansi clone ShTat1.3 procyclin EP2 (EP2) gene, complete cds.    52  0.014  1
AF353632|AF353632.1 Trypanosoma brucei brucei surface protein EP3-3 procyclin precursor (EP3-3) gene, EP3-3-GPEET/PAG3-2 allele, complete cds.    52  0.014  1
AF353631|AF353631.1 Trypanosoma brucei brucei surface protein EP3-3 procyclin precursor (EP3-3) gene, EP3-3-GPEET/PAG3-1 allele, complete cds.    52  0.014  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U66367_1(U66367|pid:none) Dictyostelium discoideum SapA (sapA) m...   135   2e-30
CR861144_1(CR861144|pid:none) Pongo abelii mRNA; cDNA DKFZp459J1...   112   3e-23
(Q6IBQ6) RecName: Full=Proactivator polypeptide; Contains:   Rec...   111   5e-23
AY892719_1(AY892719|pid:none) Synthetic construct Homo sapiens c...   111   5e-23
AB209776_1(AB209776|pid:none) Homo sapiens mRNA for prosaposin v...   110   6e-23
AK223290_1(AK223290|pid:none) Homo sapiens mRNA for prosaposin (...   110   6e-23
CR861454_1(CR861454|pid:none) Pongo abelii mRNA; cDNA DKFZp459G1...   110   6e-23
AK299184_1(AK299184|pid:none) Homo sapiens cDNA FLJ54136 complet...   109   1e-22
CR456746_1(CR456746|pid:none) Homo sapiens full open reading fra...   109   2e-22
BT045327_1(BT045327|pid:none) Salmo salar clone ssal-rgf-518-210...   107   7e-22

 psg: 0.99  gvh: 0.88  alm: 0.42  top: 0.07  tms: 0.00  mit: 0.35  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: endoplasmic reticulum
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: nuclear

>> prediction for VSD810 is exc

>VSD810F.Seq
AAACAATCAATTTAAAAAATGATGAAATTTTTAATTGTTTTAATTTTAGCATTATTCGCT
TTATCAAGTGTTAATGCAAGCAGCGCTATTGAATGCGAACTTTGTGAATATGCTGCCAAT
TATGGTGAATTGCTCATTCAATCAAATGCCACCGAAACTGAAATTATCGATAAAGTTGAA
AACTTTTGCAAAGTTATCCCATCTAGTTTCCAAGCCACTTGTGATAGTTTAATCGCAAAC
TATGGTAAACAACTCATTCAAATGATTGTCAACAAAGAATCACCAAGCACTTTATGCGCT
CAAATTGATATGTGTGCATCATCAACTGAAGCCGTCCAAGGTATTTTAGAATGTGACATT
TGCCAATTCATCGTTAAACAAGTCAACAAATATATTTCAGGTAACGCAACTGAAGCCCAA
ATCCTCAAATTCTTAGATACTGATTGTGAAGTCTTTGGTAAAGG----------

>VSD810Z.Seq
----------TTTGGTAAAGGTGGTTCAGTAACTTGTCAAAACATTGTCAACANCTATGC
CCCACAAATCATCAATTTAATCATTAACAACGCATCACCATCCCAAGTNTGCGGTCTTGT
NGGTTTATGTGGCTCCTCTTTGAAAATCGAAGAACCAGTCCAAGGTGAATTAGAATGTGG
TGTTTGTGAAGTCATTGCTCAACAATGCTCAAACTACATCAAAGCCAACAAANCCGAATC
TGAAATCGTTGGTCTCCTCGATCAATTCTGTTCAACTCTTTCAATCTTTGAAAGTGCTTG
TGATACCATCGTTGCCAGCTCTGCTCCAAAGATCATCAACTTACTCTTACAAAACCAAAG
TGCAACTGTAGTTTGTACCGAAATTGGCTTCTGTGGTAACTCTTCATCAGGTAATNACAA
CCATTCATCATCTGAAGAATCTTCATCGTCTTCCCAACCATCATCATCTTCTGAATCATC
AGAATCAAGTTACTCNGGTTCAATGACTGGTGCTTCATCTGGTTCAGCTTCAGGTTCAGG
TTCAGGTACATCNGAAACATGAAAATTTACATCAAATAAATTTAANAATTAAATAATTTA

>VSD810P.Seq
AAACAATCAATTTAAAAAATGATGAAATTTTTAATTGTTTTAATTTTAGCATTATTCGCT
TTATCAAGTGTTAATGCAAGCAGCGCTATTGAATGCGAACTTTGTGAATATGCTGCCAAT
TATGGTGAATTGCTCATTCAATCAAATGCCACCGAAACTGAAATTATCGATAAAGTTGAA
AACTTTTGCAAAGTTATCCCATCTAGTTTCCAAGCCACTTGTGATAGTTTAATCGCAAAC
TATGGTAAACAACTCATTCAAATGATTGTCAACAAAGAATCACCAAGCACTTTATGCGCT
CAAATTGATATGTGTGCATCATCAACTGAAGCCGTCCAAGGTATTTTAGAATGTGACATT
TGCCAATTCATCGTTAAACAAGTCAACAAATATATTTCAGGTAACGCAACTGAAGCCCAA
ATCCTCAAATTCTTAGATACTGATTGTGAAGTCTTTGGTAAAGG----------TTTGGT
AAAGGTGGTTCAGTAACTTGTCAAAACATTGTCAACANCTATGCCCCACAAATCATCAAT
TTAATCATTAACAACGCATCACCATCCCAAGTNTGCGGTCTTGTNGGTTTATGTGGCTCC
TCTTTGAAAATCGAAGAACCAGTCCAAGGTGAATTAGAATGTGGTGTTTGTGAAGTCATT
GCTCAACAATGCTCAAACTACATCAAAGCCAACAAANCCGAATCTGAAATCGTTGGTCTC
CTCGATCAATTCTGTTCAACTCTTTCAATCTTTGAAAGTGCTTGTGATACCATCGTTGCC
AGCTCTGCTCCAAAGATCATCAACTTACTCTTACAAAACCAAAGTGCAACTGTAGTTTGT
ACCGAAATTGGCTTCTGTGGTAACTCTTCATCAGGTAATNACAACCATTCATCATCTGAA
GAATCTTCATCGTCTTCCCAACCATCATCATCTTCTGAATCATCAGAATCAAGTTACTCN
GGTTCAATGACTGGTGCTTCATCTGGTTCAGCTTCAGGTTCAGGTTCAGGTACATCNGAA
ACATGAAAATTTACATCAAATAAATTTAANAATTAAATAATTTA

>VSD810E.Seq
AAACAATCAATTTAAAAAATGATGAAATTTTTAATTGTTTTAATTTTAGCATTATTCGCT
TTATCAAGTGTTAATGCAAGCAGCGCTATTGAATGCGAACTTTGTGAATATGCTGCCAAT
TATGGTGAATTGCTCATTCAATCAAATGCCACCGAAACTGAAATTATCGATAAAGTTGAA
AACTTTTGCAAAGTTATCCCATCTAGTTTCCAAGCCACTTGTGATAGTTTAATCGCAAAC
TATGGTAAACAACTCATTCAAATGATTGTCAACAAAGAATCACCAAGCACTTTATGCGCT
CAAATTGATATGTGTGCATCATCAACTGAAGCCGTCCAAGGTATTTTAGAATGTGACATT
TGCCAATTCATCGTTAAACAAGTCAACAAATATATTTCAGGTAACGCAACTGAAGCCCAA
ATCCTCAAATTCTTAGATACTGATTGTGAAGTCTTTGGTAAAGGTGGTTCAGTAACTTGT
CAAAACATTGTCAACANCTATGCCCCACAAATCATCAATTTAATCATTAACAACGCATCA
CCATCCCAAGTNTGCGGTCTTGTNGGTTTATGTGGCTCCTCTTTGAAAATCGAAGAACCA
GTCCAAGGTGAATTAGAATGTGGTGTTTGTGAAGTCATTGCTCAACAATGCTCAAACTAC
ATCAAAGCCAACAAANCCGAATCTGAAATCGTTGGTCTCCTCGATCAATTCTGTTCAACT
CTTTCAATCTTTGAAAGTGCTTGTGATACCATCGTTGCCAGCTCTGCTCCAAAGATCATC
AACTTACTCTTACAAAACCAAAGTGCAACTGTAGTTTGTACCGAAATTGGCTTCTGTGGT
AACTCTTCATCAGGTAATNACAACCATTCATCATCTGAAGAATCTTCATCGTCTTCCCAA
CCATCATCATCTTCTGAATCATCAGAATCAAGTTACTCNGGTTCAATGACTGGTGCTTCA
TCTGGTTCAGCTTCAGGTTCAGGTTCAGGTACATCNGAAACATGAAAATTTACATCAAAT
AAATTTAANAATTAAATAATTTA