>VSD576 (VSD576Q) /CSM/VS/VSD5-D/VSD576Q.Seq.d/
XXXXXXXXXXTCATTTAATTTGTTTCTTCCTAATCTCTGAATTGGTTTTAGGTTGGTATT
TAGCCATTTCTTTTCAAGTTAGTCATGTAGTTGAAGATCTTCAATTCATGGCAACACCTG
AAATTTTCGATGGTGCTGATCACCCATTACCAACAACCTTCAATCAAGATTGGGCAATTC
TTCAAGTTAAAACTACTCAAGATTATGCTCAAGATTCAGTTTTAAGTACTTTCTTTTCTG
GTGGTTTAAATTTACAAGTTATTCATCATTGTTTCCCAACAATTGCTCAAGATTATTACC
CACAAATTGTTCCAATTCTTAAAGAAGTTTGTAAAGAATATAATGTTACATATCATTATA
AGCCAACATTTACTGAAGCAATAAAGTCTCATATCAACTATCTTTACAAAATGGGTAATG
ATCCAGACTATGTCAGAAAACCAGTAAACAAAAACGATTAATCAAACTGTATTATCTCTT
CTTAATTTCAAATTATTAGTTTATATCTCTTTATTTTATTTTTTTAAAAAAAATTGTTTA
GAAAAATNAATCCAAGAAATAAAATCAATTTTAT

---HLICFFLISELVLGWYLAISFQVSHVVEDLQFMATPEIFDGADHPLPTTFNQDWAIL
QVKTTQDYAQDSVLSTFFSGGLNLQVIHHCFPTIAQDYYPQIVPILKEVCKEYNVTYHYK
PTFTEAIKSHINYLYKMGNDPDYVRKPVNKND*snciiss*fqiislylfilff*kklfr
kxnprnkinf

Frame A:
---HLICFFLISELVLGWYLAISFQVSHVVEDLQFMATPEIFDGADHPLPTTFNQDWAIL
QVKTTQDYAQDSVLSTFFSGGLNLQVIHHCFPTIAQDYYPQIVPILKEVCKEYNVTYHYK
PTFTEAIKSHINYLYKMGNDPDYVRKPVNKND*snciiss*fqiislylfilff*kklfr
kxnprnkinf


Frame B:
---i*fvss*slnwf*vgi*pflfklvm*lkifnswqhlkfsmvlithyqqpsikigqff
klkllkimlkiqf*vlsflvv*iyklfiivsqqllkiithklfqflkkfvknimlhiiis
qhllkq*slistiftkwvmiqtmsenq*tktinqtvlsllnfkllvyislfyffkkncle
kxiqeiksil


Frame C:
---fnlflpnl*igfrlvfshffss*scs*rssihgnt*nfrwc*spitnnlqsrlgnss
s*nysrlcsrfsfkyflfwwfkftsysslfpnncsrllptncsns*rsl*ri*cyisl*a
niy*snkvsyqlslqng**srlcqktskqkrliklyylflisny*fislyfiflkkiv*k
nxskk*nqfy

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSD576 (VSD576Q) /CSM/VS/VSD5-D/VSD576Q.Seq.d/                       1065   0.0
SFJ773 (SFJ773Q) /CSM/SF/SFJ7-D/SFJ773Q.Seq.d/                       1065   0.0
SSG614 (SSG614Q) /CSM/SS/SSG6-A/SSG614Q.Seq.d/                       1051   0.0
SFG336 (SFG336Q) /CSM/SF/SFG3-B/SFG336Q.Seq.d/                       1017   0.0
SFF147 (SFF147Q) /CSM/SF/SFF1-B/SFF147Q.Seq.d/                        993   0.0
CFB188 (CFB188Q) /CSM/CF/CFB1-D/CFB188Q.Seq.d/                        973   0.0
SSD596 (SSD596Q) /CSM/SS/SSD5-D/SSD596Q.Seq.d/                        670   0.0
VSH813 (VSH813Q) /CSM/VS/VSH8-A/VSH813Q.Seq.d/                        143   3e-33
VSF218 (VSF218Q) /CSM/VS/VSF2-A/VSF218Q.Seq.d/                        143   3e-33
VSC833 (VSC833Q) /CSM/VS/VSC8-B/VSC833Q.Seq.d/                        143   3e-33

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AB022097|AB022097.1 Dictyostelium discoideum FadB mRNA for delta 5 fatty acid desaturase, complete cds.  1021  0.0  3
BD092935|BD092935.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids in plants.    90  4e-47  5
BD082645|BD082645.1 Methods and compositions for synthesis of long chain polyunsaturated fatty acids.    90  4e-47  5
BD082630|BD082630.1 Methods and compositions for synthesis of long chain poly-unsaturated fatty acids.    90  4e-47  5
AB029311|AB029311.1 Dictyostelium discoideum Dd des5 gene for fatty acid desaturase, complete cds.    78  3e-42  5
U67558|U67558.1 Methanococcus jannaschii section 100 of 150 of the complete genome.    44  0.032  2
AW739703|AW739703.1 BRI00216 Biomphalaria glabrata (BS-90)-exposed Lambda Zap Library Biomphalaria glabrata cDNA clone RBGHA65TR, mRNA sequence.    36  0.063  3
AC009513|AC009513.2 Sequence of BAC F12P19 from Arabidopsis thaliana chromosome 1, complete sequence.    38  0.16  2
AC128871|AC128871.3 Rattus norvegicus clone CH230-502F22, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    46  0.19  3
AC022520|AC022520.2 Arabidopsis thaliana chromosome I BAC F8L10 genomic sequence, complete sequence.    44  0.20  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(O96099) RecName: Full=Delta(5) fatty acid desaturase B;        ...   316   2e-85
(Q9Y1W0) RecName: Full=Delta(5) fatty acid desaturase A;        ...   222   6e-57
(Q1ZXQ5) RecName: Full=Probable Delta(5) fatty acid desaturase C...   159   4e-38
AF419297_1(AF419297|pid:none) Pythium irregulare delta-5 fatty a...   117   2e-25
EF678404_1(EF678404|pid:none) Picea sitchensis clone WS0291_A04 ...   117   2e-25
AB188307_1(AB188307|pid:none) Mortierella alpina gene for delta5...   117   2e-25
(O74212) RecName: Full=Delta(5) fatty acid desaturase;          ...   116   4e-25
CP000592_278(CP000592|pid:none) Ostreococcus lucimarinus CCE9901...   104   2e-21
CQ831428_1(CQ831428|pid:none) Sequence 9 from Patent WO2004057001.    101   2e-20
CP001574_328(CP001574|pid:none) Micromonas sp. RCC299 chromosome...   100   5e-20

 psg: 0.75  gvh: 0.72  alm: 0.27  top: 0.33  tms: 0.07  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: cytoplasmic
	 24.0 %: endoplasmic reticulum
	 16.0 %: nuclear
	 12.0 %: mitochondrial
	 12.0 %: Golgi
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VSD576 is cyt

>VSD576Z.Seq
----------TCATTTAATTTGTTTCTTCCTAATCTCTGAATTGGTTTTAGGTTGGTATT
TAGCCATTTCTTTTCAAGTTAGTCATGTAGTTGAAGATCTTCAATTCATGGCAACACCTG
AAATTTTCGATGGTGCTGATCACCCATTACCAACAACCTTCAATCAAGATTGGGCAATTC
TTCAAGTTAAAACTACTCAAGATTATGCTCAAGATTCAGTTTTAAGTACTTTCTTTTCTG
GTGGTTTAAATTTACAAGTTATTCATCATTGTTTCCCAACAATTGCTCAAGATTATTACC
CACAAATTGTTCCAATTCTTAAAGAAGTTTGTAAAGAATATAATGTTACATATCATTATA
AGCCAACATTTACTGAAGCAATAAAGTCTCATATCAACTATCTTTACAAAATGGGTAATG
ATCCAGACTATGTCAGAAAACCAGTAAACAAAAACGATTAATCAAACTGTATTATCTCTT
CTTAATTTCAAATTATTAGTTTATATCTCTTTATTTTATTTTTTTAAAAAAAATTGTTTA
GAAAAATNAATCCAAGAAATAAAATCAATTTTAT