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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSD543
Library VS
(Link to library)
Clone ID VSD543
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16504-1
Original site URL
Representative seq. ID VSD543E
(Link to Original site)
Representative DNA sequence 
>VSD543 (VSD543Q) /CSM/VS/VSD5-B/VSD543Q.Seq.d/
ATTTTTTATAAAAAAAGAATGGAAGAAATAAAAGAAACTGTTGAAAAAAAAAATAAAGAA
AAAAAGTATTTAAATGATTATAAAATAATTTCTGAAGGTAATAATATTAAAGTTTTACAA
AAAAAAGATTTCAAACCAAAATTTTCATTGGTATCATATAAAAATTATGAACAAAATTTT
GATTTTGAAAATGCTAAAATTGTTTCAACCAATTTTGCCATCGATCGTCATATAAAGATG
AAGAATCCACCAGATCCATTTGTATTTATTAAAATGCCATCTTCAAAGCTTGTTCAAAGT
CAGTTAAAACTTTACTATGAAAATGAAGGTTATAAGGATATTCCAGTATGGATTACACCA
TGTTCAGCATTTATGAGATACATTAAATCGTATCCAATTGTTGGAACTGTAACAGAGACA
ATGGAAAAGAAAAAAGGTTTCACCAATAGATTCTTATCAACTAGTGAAACCAAAGCATCA
GTTTCAGTCGGATTCTTTGGTTGTGAATCATCGATGGAAGTTACAAGTGGTTATGAATAT
GAAGACACTGTTACATCTGAAGAAACTAGAAGTTGGAGTCAAACTTTAAATGAAGGTTCT
TATATTGTTTATCAAAACGTTTTAGTTTATGCTTATATAATCTATGGTGGTAGTTATAAA
CCATACATTGATCAAATGAATAAATTTAATCCTGGATTAAATATCAAATTCTTTAGAGAA
GATAAATGTATCTTTTTTGTACCAATTAATCGTGATGATGCCTTCACTCTTCGTTATCAA
GATAATACATGGGATCCAGTTGAATATGATGTTTTAATTGATTACTTGGGTCAAAATCAA
GATAAATGGTTTTCTGGTGGTTTACCATGAAAATAATAATAATAATAATAATAATAATTA
TATAAATTACATAAAGGTTTCACTATTGGTGTTTTAATCCNGGTATAAATAAAATTCTAA
AAATAAAAAATAAAAAACTA
sequence update 2001. 3.25
Translated Amino Acid sequence 
IFYKKRMEEIKETVEKKNKEKKYLNDYKIISEGNNIKVLQKKDFKPKFSLVSYKNYEQNF
DFENAKIVSTNFAIDRHIKMKNPPDPFVFIKMPSSKLVQSQLKLYYENEGYKDIPVWITP
CSAFMRYIKSYPIVGTVTETMEKKKGFTNRFLSTSETKASVSVGFFGCESSMEVTSGYEY
EDTVTSEETRSWSQTLNEGSYIVYQNVLVYAYIIYGGSYKPYIDQMNKFNPGLNIKFFRE
DKCIFFVPINRDDAFTLRYQDNTWDPVEYDVLIDYLGQNQDKWFSGGLP*k********l
yklhkgftigvlixv*ikf*k*kikn
Translated Amino Acid sequence (All Frames) 
Frame A:
IFYKKRMEEIKETVEKKNKEKKYLNDYKIISEGNNIKVLQKKDFKPKFSLVSYKNYEQNF
DFENAKIVSTNFAIDRHIKMKNPPDPFVFIKMPSSKLVQSQLKLYYENEGYKDIPVWITP
CSAFMRYIKSYPIVGTVTETMEKKKGFTNRFLSTSETKASVSVGFFGCESSMEVTSGYEY
EDTVTSEETRSWSQTLNEGSYIVYQNVLVYAYIIYGGSYKPYIDQMNKFNPGLNIKFFRE
DKCIFFVPINRDDAFTLRYQDNTWDPVEYDVLIDYLGQNQDKWFSGGLP*k********l
yklhkgftigvlixv*ikf*k*kikn


Frame B:
ffikkewkk*kkllkkkikkksi*miik*flkviilkfykkkisnqnfhwyhikimnkil
ilkmlklfqpilpsivi*r*rihqihlyllkchlqslfkvs*nftmkmkvirifqyglhh
vqhl*dtlnriqllel*qrqwkrkkvspidsyqlvkpkhqfqsdslvvnhrwklqvvmnm
ktllhlkklevgvkl*mkvlilfiktf*fmli*smvvvinhtlik*inlild*isnslek
invsflyqlivmmpslfvikiihgiqlnmmf*litwvkikingflvvyhennnnnnnnny
inyikvsllvf*sxyk*nsknkk*kt


Frame C:
fl*kkngrnkrnc*kkk*rkkvfk*l*nnf*r**y*sftkkrfqtkifigii*kl*tkf*
f*kc*ncfnqfchrssykdeestrsiciy*naifkacsksvktll*k*rl*gyssmdytm
fsiyeih*ivsncwncnrdngkekrfhq*ilin**nqsisfsrilwl*iidgsykwl*i*
rhcyi*rn*klesnfk*rflyclskrfslclynlww*l*tih*sne*i*swikyqil*rr
*mylfctn*s**clhsslsr*ymgss*i*cfn*llgsksr*mvfwwftmkiiiiiiiiii
*it*rfhywcfnpginkilkiknkkl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSD543 (VSD543Q) /CSM/VS/VSD5-B/VSD543Q.Seq.d/                       1550   0.0
VFC716 (VFC716Q) /CSM/VF/VFC7-A/VFC716Q.Seq.d/                       1550   0.0
VSJ730 (VSJ730Q) /CSM/VS/VSJ7-B/VSJ730Q.Seq.d/                       1544   0.0
VSI168 (VSI168Q) /CSM/VS/VSI1-C/VSI168Q.Seq.d/                       1542   0.0
VHP648 (VHP648Q) /CSM/VH/VHP6-B/VHP648Q.Seq.d/                       1542   0.0
VFJ763 (VFJ763Q) /CSM/VF/VFJ7-C/VFJ763Q.Seq.d/                       1538   0.0
VFI758 (VFI758Q) /CSM/VF/VFI7-C/VFI758Q.Seq.d/                       1538   0.0
VSF255 (VSF255Q) /CSM/VS/VSF2-C/VSF255Q.Seq.d/                       1530   0.0
VSF487 (VSF487Q) /CSM/VS/VSF4-D/VSF487Q.Seq.d/                       1524   0.0
VHB403 (VHB403Q) /CSM/VH/VHB4-A/VHB403Q.Seq.d/                       1522   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U48706|U48706.1 Dictyostelium discoideum small aggregate formation (smlA) gene, complete cds.  1298  0.0  5
M19469|M19469.1 Dictyostelium discoideum 109 gene 3, complete cds.   327  e-108  2
X94341|X94341.1 D.discoideum mRNA for unknown protein with homology to spore germination protein.    86  8e-52  6
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.          36  0.019  12
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    34  0.18  12
BQ596151|BQ596151.1 PfESToab29h06.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5', mRNA sequence.    40  0.25  2
BQ633152|BQ633152.1 PfESToab38d10.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5', mRNA sequence.    40  0.26  2
AC095627|AC095627.7 Rattus norvegicus clone CH230-8N23, WORKING DRAFT SEQUENCE, 2 unordered pieces.    40  0.30  2
CA930567|CA930567.1 MTU4CA.P24.B01 Aspen apex cDNA Library Populus tremuloides cDNA, mRNA sequence.    34  0.78  2
CA930485|CA930485.1 MTU4CA.P23.B04 Aspen apex cDNA Library Populus tremuloides cDNA, mRNA sequence.    34  0.78  2
dna update 2003. 9.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116330_42(AC116330|pid:none) Dictyostelium discoideum chromoso...    67   1e-09
AC116330_41(AC116330|pid:none) Dictyostelium discoideum chromoso...    50   2e-04
CP000712_1048(CP000712|pid:none) Pseudomonas putida F1, complete...    42   0.025
CP001083_1669(CP001083|pid:none) Clostridium botulinum Ba4 str. ...    38   0.61
DQ790236_1(DQ790236|pid:none) Champereia manillana acetyl co-A c...    37   0.80
DQ790237_1(DQ790237|pid:none) Agonandra macrocarpa acetyl co-A c...    37   1.4
AE001362_167(AE001362|pid:none) Plasmodium falciparum 3D7 chromo...    37   1.4
CU207366_1354(CU207366|pid:none) Gramella forsetii KT0803 comple...    37   1.4
AE009951_1192(AE009951|pid:none) Fusobacterium nucleatum subsp. ...    36   1.8
AY332625_22(AY332625|pid:none) Campylobacter jejuni serotype HS:...    35   3.0
protein update 2009. 7.29
PSORT 

 psg: 0.30  gvh: 0.11  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.24  mip: 0.02
 nuc: 0.13  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: nuclear
	 28.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: plasma membrane

>> prediction for VSD543 is nuc
5' end seq. ID VSD543F
5' end seq. 
>VSD543F.Seq
ATTTTTTATAAAAAAAGAATGGAAGAAATAAAAGAAACTGTTGAAAAAAAAAATAAAGAA
AAAAAGTATTTAAATGATTATAAAATAATTTCTGAAGGTAATAATATTAAAGTTTTACAA
AAAAAAGATTTCAAACCAAAATTTTCATTGGTATCATATAAAAATTATGAACAAAATTTT
GATTTTGAAAATGCTAAAATTGTTTCAACCAATTTTGCCATCGATCGTCATATAAAGATG
AAGAATCCACCAGATCCATTTGTATTTATTAAAATGCCATCTTCAAAGCTTGTTCAAAGT
CAGTTAAAACTTTACTATGAAAATGAAGGTTATAAGGATATTCCAGTATGGATTACACCA
TGTTCAGCATTTATGAGATACATTAAATCGTATCCAATTGTTGGAACTGTAACAGAGACA
ATGGAAAAGAAAAAAGGTTTCACCAATAGATTCTTATCAACTAGNGAAACCAAAGCATCA
GTTTCAGTCGGATTCTTTGGTTGTGAATCATCGATGGAAGTTA----------
Length of 5' end seq. 523
3' end seq. ID VSD543Z
3' end seq. 
>VSD543Z.Seq
----------GAAGGTTATAAGGATATTCCAGTATGGATTACACCATGTTCAGCATTTAT
GAGATACATTAAATCGTATCCAATTGTTGGAACTGTAACAGAGACAATGGAAAAGAAAAA
AGGTTTCACCAATAGATTCTTATCAACTAGTGAAACCAAAGCATCAGTTTCAGTCGGATT
CTTTGGTTGTGAATCATCGATGGAAGTTACAAGTGGTTATGAATATGAAGACACTGTTAC
ATCTGAAGAAACTAGAAGTTGGAGTCAAACTTTAAATGAAGGTTCTTATATTGTTTATCA
AAACGTTTTAGTTTATGCTTATATAATCTATGGTGGTAGTTATAAACCATACATTGATCA
AATGAATAAATTTAATCCTGGATTAAATATCAAATTCTTTAGAGAAGATAAATGTATCTT
TTTTGTACCAATTAATCGTGATGATGCCTTCACTCTTCGTTATCAAGATAATACATGGGA
TCCAGTTGAATATGATGTTTTAATTGATTACTTGGGTCAAAATCAAGATAAATGGTTTTC
TGGTGGTTTACCATGAAAATAATAATAATAATAATAATAATAATTATATAAATTACATAA
AGGTTTCACTATTGGTGTTTTAATCCNGGTATAAATAAAATTCTAAAAATAAAAAATAAA
AAACTA
Length of 3' end seq. 656
Connected seq. ID VSD543P
Connected seq. 
>VSD543P.Seq
ATTTTTTATAAAAAAAGAATGGAAGAAATAAAAGAAACTGTTGAAAAAAAAAATAAAGAA
AAAAAGTATTTAAATGATTATAAAATAATTTCTGAAGGTAATAATATTAAAGTTTTACAA
AAAAAAGATTTCAAACCAAAATTTTCATTGGTATCATATAAAAATTATGAACAAAATTTT
GATTTTGAAAATGCTAAAATTGTTTCAACCAATTTTGCCATCGATCGTCATATAAAGATG
AAGAATCCACCAGATCCATTTGTATTTATTAAAATGCCATCTTCAAAGCTTGTTCAAAGT
CAGTTAAAACTTTACTATGAAAATGAAGGTTATAAGGATATTCCAGTATGGATTACACCA
TGTTCAGCATTTATGAGATACATTAAATCGTATCCAATTGTTGGAACTGTAACAGAGACA
ATGGAAAAGAAAAAAGGTTTCACCAATAGATTCTTATCAACTAGNGAAACCAAAGCATCA
GTTTCAGTCGGATTCTTTGGTTGTGAATCATCGATGGAAGTTA----------GAAGGTT
ATAAGGATATTCCAGTATGGATTACACCATGTTCAGCATTTATGAGATACATTAAATCGT
ATCCAATTGTTGGAACTGTAACAGAGACAATGGAAAAGAAAAAAGGTTTCACCAATAGAT
TCTTATCAACTAGTGAAACCAAAGCATCAGTTTCAGTCGGATTCTTTGGTTGTGAATCAT
CGATGGAAGTTACAAGTGGTTATGAATATGAAGACACTGTTACATCTGAAGAAACTAGAA
GTTGGAGTCAAACTTTAAATGAAGGTTCTTATATTGTTTATCAAAACGTTTTAGTTTATG
CTTATATAATCTATGGTGGTAGTTATAAACCATACATTGATCAAATGAATAAATTTAATC
CTGGATTAAATATCAAATTCTTTAGAGAAGATAAATGTATCTTTTTTGTACCAATTAATC
GTGATGATGCCTTCACTCTTCGTTATCAAGATAATACATGGGATCCAGTTGAATATGATG
TTTTAATTGATTACTTGGGTCAAAATCAAGATAAATGGTTTTCTGGTGGTTTACCATGAA
AATAATAATAATAATAATAATAATAATTATATAAATTACATAAAGGTTTCACTATTGGTG
TTTTAATCCNGGTATAAATAAAATTCTAAAAATAAAAAATAAAAAACTA
Length of connected seq. 1179
Full length Seq ID VSD543E
Full length Seq. 
>VSD543E.Seq
ATTTTTTATAAAAAAAGAATGGAAGAAATAAAAGAAACTGTTGAAAAAAAAAATAAAGAA
AAAAAGTATTTAAATGATTATAAAATAATTTCTGAAGGTAATAATATTAAAGTTTTACAA
AAAAAAGATTTCAAACCAAAATTTTCATTGGTATCATATAAAAATTATGAACAAAATTTT
GATTTTGAAAATGCTAAAATTGTTTCAACCAATTTTGCCATCGATCGTCATATAAAGATG
AAGAATCCACCAGATCCATTTGTATTTATTAAAATGCCATCTTCAAAGCTTGTTCAAAGT
CAGTTAAAACTTTACTATGAAAATGAAGGTTATAAGGATATTCCAGTATGGATTACACCA
TGTTCAGCATTTATGAGATACATTAAATCGTATCCAATTGTTGGAACTGTAACAGAGACA
ATGGAAAAGAAAAAAGGTTTCACCAATAGATTCTTATCAACTAGTGAAACCAAAGCATCA
GTTTCAGTCGGATTCTTTGGTTGTGAATCATCGATGGAAGTTACAAGTGGTTATGAATAT
GAAGACACTGTTACATCTGAAGAAACTAGAAGTTGGAGTCAAACTTTAAATGAAGGTTCT
TATATTGTTTATCAAAACGTTTTAGTTTATGCTTATATAATCTATGGTGGTAGTTATAAA
CCATACATTGATCAAATGAATAAATTTAATCCTGGATTAAATATCAAATTCTTTAGAGAA
GATAAATGTATCTTTTTTGTACCAATTAATCGTGATGATGCCTTCACTCTTCGTTATCAA
GATAATACATGGGATCCAGTTGAATATGATGTTTTAATTGATTACTTGGGTCAAAATCAA
GATAAATGGTTTTCTGGTGGTTTACCATGAAAATAATAATAATAATAATAATAATAATTA
TATAAATTACATAAAGGTTTCACTATTGGTGTTTTAATCCNGGTATAAATAAAATTCTAA
AAATAAAAAATAAAAAACTA
Length of full length seq. 980