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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSD514
Library VS
(Link to library)
Clone ID VSD514
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16297-1
Original site URL
Representative seq. ID VSD514E
(Link to Original site)
Representative DNA sequence 
>VSD514 (VSD514Q) /CSM/VS/VSD5-A/VSD514Q.Seq.d/
CAAAATGATGGTATCAAATTAAGTTATTTTTCAATCAAAAAAAGGTTCTAATTAAAAAAA
CTTTTGGAAAGTCGCTATCACTAACTTTGTGCACAGATATTTCCATTCACAGTATATAAT
AATTACACTAAATTTTAATACCTATCAAAAACATGGATTTATATTTTTTTTTTTTTAATA
TTTTAAGATAAATCATTGTTTATTAAATCCAATAAACTATATTATTTTATAATTTATAAA
AAAAAAGTCAAATATCTCCAAACTCGTCTCTATTGGTCTTTATCTTTCTTTAAAAACTTA
AATCATTGTTTACNAATTGCAGTAAACAACATTTCGTTACAGTTAAGGGAAATAATATTC
AAAAGAACAAACAAAAACAAAAACAACAACAGCAACAACAAATTCTTTCAAACTTCTCTC
TCATGTGCTGTCTCTTTTCTTTAAAAACTTAAATCACAGTTTACAAATTGCAGTAAGACT
ATATTTCGTTACAATCAAAGGAAAAAATGGTGCAAAGAACAAAAATAAAAAGAACAACAA
CAACAACAACAACAACAACAACNACAACAACAACAACAACAACAACAATTCTGTCAAACN
TGTTTCTC
sequence update 2001. 3.23
Translated Amino Acid sequence 
qndgiklsyfsikkrf*lkkllesryh*lcaqifpftvynnytkf*ylsktwiyifffli
f*DKSLFIKSNKLYYFIIYKKKVKYLQTRLYWSLSFFKNLNHCLXIAVNNISLQLREIIF
KRTNKNKNNNSNNKFFQTSLSCAVSFL*klksqftncsktifrynqrkkwckeqk*keqq
qqqqqqqxqqqqqqqqfcqtcf
Translated Amino Acid sequence (All Frames) 
Frame A:
qndgiklsyfsikkrf*lkkllesryh*lcaqifpftvynnytkf*ylsktwiyifffli
f*DKSLFIKSNKLYYFIIYKKKVKYLQTRLYWSLSFFKNLNHCLXIAVNNISLQLREIIF
KRTNKNKNNNSNNKFFQTSLSCAVSFL*klksqftncsktifrynqrkkwckeqk*keqq
qqqqqqqxqqqqqqqqfcqtcf


Frame B:
kmmvsn*vifqskkgsn*knfwkvaitnfvhryfhsqyiiitlnfntyqkhgfiffff*y
fkinhcllnpinyiil*fikkksnisklvsiglylslkt*iivyxlq*ttfrys*gk*ys
keqtktktttattnsfkllshvlslffknlnhslqiavrlyfvtikgkngaknknkknnn
nnnnnnnxnnnnnnnnsvkxvs


Frame C:
k*wyqiklffnqkkvlikktfgkslsltlctdisihsi**lh*ilipiknmdlyffffni
lr*iivy*iq*tilfynl*kksqispnssllvfifl*klkslftncskqhfvtvkgnniq
knkqkqkqqqqqqilsnfslmcclfslkt*itvyklq*dyislqskekmvqrtkikrttt
tttttttttttttttilsnxfl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSD514 (VSD514Q) /CSM/VS/VSD5-A/VSD514Q.Seq.d/                        303   2e-81
SSM140 (SSM140Q) /CSM/SS/SSM1-B/SSM140Q.Seq.d/                        303   2e-81
SSK650 (SSK650Q) /CSM/SS/SSK6-C/SSK650Q.Seq.d/                        303   2e-81
SSF493 (SSF493Q) /CSM/SS/SSF4-D/SSF493Q.Seq.d/                        303   2e-81
SSE802 (SSE802Q) /CSM/SS/SSE8-A/SSE802Q.Seq.d/                        303   2e-81
VSH261 (VSH261Q) /CSM/VS/VSH2-C/VSH261Q.Seq.d/                        297   1e-79
SSL534 (SSL534Q) /CSM/SS/SSL5-B/SSL534Q.Seq.d/                        293   2e-78
SSF315 (SSF315Q) /CSM/SS/SSF3-A/SSF315Q.Seq.d/                        293   2e-78
VSJ521 (VSJ521Q) /CSM/VS/VSJ5-A/VSJ521Q.Seq.d/                        240   2e-62
VSI138 (VSI138Q) /CSM/VS/VSI1-B/VSI138Q.Seq.d/                        240   2e-62
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC114303|AC114303.2 Homo sapiens chromosome 5 clone RP11-157J11, complete sequence.    36  0.075  4
AC095704|AC095704.6 Rattus norvegicus clone CH230-9B23, WORKING DRAFT SEQUENCE, 5 unordered pieces.    38  0.11  3
AC114530|AC114530.3 Rattus norvegicus clone CH230-57A9, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    38  0.31  3
AC131999|AC131999.3 Mus musculus clone RP24-139E21, WORKING DRAFT SEQUENCE, 6 unordered pieces.    38  0.50  4
BX247906|BX247906.4 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone  DKEY-9O22.    46  0.50  1
BX276096|BX276096.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-240J18.    38  0.97  4
AF037220|AF037220.1 Mesembryanthemum crystallinum inositol monophosphatase (IMP1) mRNA, complete cds.    32  1.1  3
AC093980|AC093980.5 Rattus norvegicus clone CH230-30J22, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    34  1.2  6
AX348594|AX348594.1 Sequence 52 from Patent WO0202807.            38  1.6  4
AX281384|AX281384.1 Sequence 48 from Patent WO0177376.            38  1.6  4
dna update 2003. 9.20
Homology vs Protein 

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3268448
Number of Hits to DB: 685,502,187
protein update 2009. 7.29
PSORT 

 psg: 0.77  gvh: 0.37  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.36  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: mitochondrial
	 28.0 %: cytoplasmic
	 28.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VSD514 is mit
5' end seq. ID VSD514F
5' end seq. 
>VSD514F.Seq
CAAAATGATGGTATCAAATTAAGTTATTTTTCAATCAAAAAAAGGTTCTAATTAAAAAAA
CTTTTGGAAAGTCGCTATCACTAACTTTGTGCACAGATATTTCCATTCACAGTATATAAT
AATTACACTAAATTTTAATACCTATCAAAAACATGGATTTATATTTTTTTTTTTTTAATA
TTTNAAGATAAATCATNGTTTATTAAATCCAATAANCTATNTTATTTTATAATTTATAAA
AAAAANGTC----------
Length of 5' end seq. 249
3' end seq. ID VSD514Z
3' end seq. 
>VSD514Z.Seq
----------TTTTTTTTTTTTTAATATTTTAAGATAAATCATTGTTTATTAAATCCAAT
AAACTATATTATTTTATAATTTATAAAAAAAAAGTCAAATATCTCCAAACTCGTCTCTAT
TGGTCTTTATCTTTCTTTAAAAACTTAAATCATTGTTTACNAATTGCAGTAAACAACATT
TCGTTACAGTTAAGGGAAATAATATTCAAAAGAACAAACAAAAACAAAAACAACAACAGC
AACAACAAATTCTTTCAAACTTCTCTCTCATGTGCTGTCTCTTTTCTTTAAAAACTTAAA
TCACAGTTTACAAATTGCAGTAAGACTATATTTCGTTACAATCAAAGGAAAAAATGGTGC
AAAGAACAAAAATAAAAAGAACAACAACAACAACAACAACAACAACAACNACAACAACAA
CAACAACAACAACAATTCTGTCAAACNTGTTTCTC
Length of 3' end seq. 445
Connected seq. ID VSD514P
Connected seq. 
>VSD514P.Seq
CAAAATGATGGTATCAAATTAAGTTATTTTTCAATCAAAAAAAGGTTCTAATTAAAAAAA
CTTTTGGAAAGTCGCTATCACTAACTTTGTGCACAGATATTTCCATTCACAGTATATAAT
AATTACACTAAATTTTAATACCTATCAAAAACATGGATTTATATTTTTTTTTTTTTAATA
TTTNAAGATAAATCATNGTTTATTAAATCCAATAANCTATNTTATTTTATAATTTATAAA
AAAAANGTC----------TTTTTTTTTTTTTAATATTTTAAGATAAATCATTGTTTATT
AAATCCAATAAACTATATTATTTTATAATTTATAAAAAAAAAGTCAAATATCTCCAAACT
CGTCTCTATTGGTCTTTATCTTTCTTTAAAAACTTAAATCATTGTTTACNAATTGCAGTA
AACAACATTTCGTTACAGTTAAGGGAAATAATATTCAAAAGAACAAACAAAAACAAAAAC
AACAACAGCAACAACAAATTCTTTCAAACTTCTCTCTCATGTGCTGTCTCTTTTCTTTAA
AAACTTAAATCACAGTTTACAAATTGCAGTAAGACTATATTTCGTTACAATCAAAGGAAA
AAATGGTGCAAAGAACAAAAATAAAAAGAACAACAACAACAACAACAACAACAACAACNA
CAACAACAACAACAACAACAACAATTCTGTCAAACNTGTTTCTC
Length of connected seq. 694
Full length Seq ID VSD514E
Full length Seq. 
>VSD514E.Seq
CAAAATGATGGTATCAAATTAAGTTATTTTTCAATCAAAAAAAGGTTCTAATTAAAAAAA
CTTTTGGAAAGTCGCTATCACTAACTTTGTGCACAGATATTTCCATTCACAGTATATAAT
AATTACACTAAATTTTAATACCTATCAAAAACATGGATTTATATTTTTTTTTTTTTAATA
TTTTAAGATAAATCATTGTTTATTAAATCCAATAAACTATATTATTTTATAATTTATAAA
AAAAAAGTCAAATATCTCCAAACTCGTCTCTATTGGTCTTTATCTTTCTTTAAAAACTTA
AATCATTGTTTACNAATTGCAGTAAACAACATTTCGTTACAGTTAAGGGAAATAATATTC
AAAAGAACAAACAAAAACAAAAACAACAACAGCAACAACAAATTCTTTCAAACTTCTCTC
TCATGTGCTGTCTCTTTTCTTTAAAAACTTAAATCACAGTTTACAAATTGCAGTAAGACT
ATATTTCGTTACAATCAAAGGAAAAAATGGTGCAAAGAACAAAAATAAAAAGAACAACAA
CAACAACAACAACAACAACAACNACAACAACAACAACAACAACAACAATTCTGTCAAACN
TGTTTCTC
Length of full length seq. 608