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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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VSD487
Library VS
(Link to library)
Clone ID VSD487
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VSD487P
(Link to Original site)
Representative DNA sequence 
>VSD487 (VSD487Q) /CSM/VS/VSD4-D/VSD487Q.Seq.d/
CGCGTCCGCCAACCCATTAGCCTCAGCCCCAGGTTCAATTCGTGGTGATTTCGGTGTTGA
TGTTGGTAGAAACATCATCCACGGTTCTGATTCAGTTGAATCTGCCAACAGAGAAATTGC
TTTATGGTTCAAACCAGAAGAATTATTAACTGAAGTTAAACCAAACCCAAATTTATACGA
ATAAATAATTTAGCTAAATTCTAAATCATTTAATGTAAAAAAAAAAXXXXXXXXXXCCAA
CCCATTAGCCTCAGCCCCAGGTTCAATTCGTGGTGATTTCGGTGTTGATGTTGGTAGAAA
CATCATCCACGGTTCTGATTCAGTTGAATCTGCCAACAGAGAAATTGCTTTATGGTTCAA
ACCAGAAGAATTATTAACTGAAGTTAAACCAAACCCAAATTTATACGAATAAATATTTAG
CTAATTCTAATCATTTAA
sequence update 2001. 3.22
Translated Amino Acid sequence 
asanplasapgsirgdfgvdvgrniihgsdsvesanreialwfkpeelltevkpnpnlye
*ii*LNSKSFNVKKK---

---NPLASAPGSIRGDFGVDVGRNIIHGSDSVESANREIALWFKPEELLTEVKPNPNLYE
*ifs*f*sf
Translated Amino Acid sequence (All Frames) 
Frame A:
rvrqpislsprfnsw*frc*cw*khhprf*fs*icqqrncfmvqtrriin*s*tkpkfir
innlakf*ii*ckkk---

---pth*pqpqvqfvvisvlmlvetsstvliqlnlptekllygsnqkny*lklnqtqiyt
nkylansnhl

Frame B:
asanplasapgsirgdfgvdvgrniihgsdsvesanreialwfkpeelltevkpnpnlye
*ii*LNSKSFNVKKK---

---qpislsprfnsw*frc*cw*khhprf*fs*icqqrncfmvqtrriin*s*tkpkfir
ini*lilii*

Frame C:
rppth*pqpqvqfvvisvlmlvetsstvliqlnlptekllygsnqkny*lklnqtqiytn
k*fs*ilnhlm*kk---

---NPLASAPGSIRGDFGVDVGRNIIHGSDSVESANREIALWFKPEELLTEVKPNPNLYE
*ifs*f*sf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSD487 (VSD487Q) /CSM/VS/VSD4-D/VSD487Q.Seq.d/                        428   e-119
VSJ739 (VSJ739Q) /CSM/VS/VSJ7-B/VSJ739Q.Seq.d/                        412   e-114
VSJ480 (VSJ480Q) /CSM/VS/VSJ4-D/VSJ480Q.Seq.d/                        412   e-114
VSF701 (VSF701Q) /CSM/VS/VSF7-A/VSF701Q.Seq.d/                        412   e-114
VHN707 (VHN707Q) /CSM/VH/VHN7-A/VHN707Q.Seq.d/                        412   e-114
VHK695 (VHK695Q) /CSM/VH/VHK6-D/VHK695Q.Seq.d/                        412   e-114
SSC291 (SSC291Q) /CSM/SS/SSC2-D/SSC291Q.Seq.d/                        412   e-114
SLE365 (SLE365Q) /CSM/SL/SLE3-C/SLE365Q.Seq.d/                        412   e-114
SLD152 (SLD152Q) /CSM/SL/SLD1-C/SLD152Q.Seq.d/                        412   e-114
FC-AH03 (FC-AH03Q) /CSM/FC/FC-AH/FC-AH03Q.Seq.d/                      412   e-114
own update 2003. 7. 5
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

A32505|A32505.1 D.discoideum NDP kinase gene.                    412  e-111  1
J05457|J05457.1 D.discoideum nucleoside diphosphate kinase Gip17 mRNA, complete cds.   412  e-111  1
L23067|L23067.1 Dictyostelium discoideum nucleoside diphosphate kinase (ndkC) gene, complete cds.   347  e-104  2
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.   347  7e-96  6
BQ108400|BQ108400.1 shrpID000721 LvG Litopenaeus vannamei cDNA, mRNA sequence.    54  0.001  1
AX300907|AX300907.1 Sequence 13 from Patent WO0185989.            52  0.006  1
BM401306|BM401306.1 5009-0-85-E09.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    50  0.023  1
BM394150|BM394150.1 50072-2-2-C03.f.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    50  0.023  1
BM392825|BM392825.1 50071-2-2-C03.f.1 Chilcoat/Turkewitz cDNA (small fraction) Tetrahymena thermophila cDNA, mRNA sequence.    50  0.023  1
BM394908|BM394908.1 50072-2-6-F02.r.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    50  0.023  1
dna update 2003. 8.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NRL(1NDC) Nucleoside diphosphate kinase (EC 2.7.4.6) complexed W...   119   3e-26
(P22887) RecName: Full=Nucleoside diphosphate kinase, cytosolic;...   119   3e-26
NRL(1NDK) Nucleoside diphosphate kinase (EC 2.7.4.6) mutant with...   115   5e-25
(Q7Z8P9) RecName: Full=Nucleoside diphosphate kinase;          S...    86   5e-16
CU640366_961(CU640366|pid:none) Podospora anserina genomic DNA c...    84   1e-15
CR380959_578(CR380959|pid:none) Candida glabrata strain CBS138 c...    82   5e-15
D88148_1(D88148|pid:none) Neurospora crassa mRNA for nucleoside ...    82   5e-15
FM992692_265(FM992692|pid:none) Candida dubliniensis CD36 chromo...    81   1e-14
DQ907945_1(DQ907945|pid:none) Litopenaeus vannamei oncoprotein n...    81   1e-14
(B0C4I0) RecName: Full=Nucleoside diphosphate kinase;          S...    81   1e-14
protein update 2009. 7.29
PSORT 

 psg: 0.87  gvh: 0.41  alm: 0.54  top: 0.67  tms: 0.00  mit: 0.31  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 28.0 %: cytoplasmic
	 28.0 %: nuclear
	 24.0 %: mitochondrial
	 12.0 %: cytoskeletal
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for VSD487 is cyt
5' end seq. ID VSD487F
5' end seq. 
>VSD487F.Seq
CGCGTCCGCCAACCCATTAGCCTCAGCCCCAGGTTCAATTCGTGGTGATTTCGGTGTTGA
TGTTGGTAGAAACATCATCCACGGTTCTGATTCAGTTGAATCTGCCAACAGAGAAATTGC
TTTATGGTTCAAACCAGAAGAATTATTAACTGAAGTTAAACCAAACCCAAATTTATACGA
ATAAATAATTTAGCTAAATTCTAAATCATTTAATGTAAAAAAAAAA----------
Length of 5' end seq. 226
3' end seq. ID VSD487Z
3' end seq. 
>VSD487Z.Seq
----------CCAACCCATTAGCCTCAGCCCCAGGTTCAATTCGTGGTGATTTCGGTGTT
GATGTTGGTAGAAACATCATCCACGGTTCTGATTCAGTTGAATCTGCCAACAGAGAAATT
GCTTTATGGTTCAAACCAGAAGAATTATTAACTGAAGTTAAACCAAACCCAAATTTATAC
GAATAAATATTTAGCTAATTCTAATCATTTAA
Length of 3' end seq. 202
Connected seq. ID VSD487P
Connected seq. 
>VSD487P.Seq
CGCGTCCGCCAACCCATTAGCCTCAGCCCCAGGTTCAATTCGTGGTGATTTCGGTGTTGA
TGTTGGTAGAAACATCATCCACGGTTCTGATTCAGTTGAATCTGCCAACAGAGAAATTGC
TTTATGGTTCAAACCAGAAGAATTATTAACTGAAGTTAAACCAAACCCAAATTTATACGA
ATAAATAATTTAGCTAAATTCTAAATCATTTAATGTAAAAAAAAAA----------CCAA
CCCATTAGCCTCAGCCCCAGGTTCAATTCGTGGTGATTTCGGTGTTGATGTTGGTAGAAA
CATCATCCACGGTTCTGATTCAGTTGAATCTGCCAACAGAGAAATTGCTTTATGGTTCAA
ACCAGAAGAATTATTAACTGAAGTTAAACCAAACCCAAATTTATACGAATAAATATTTAG
CTAATTCTAATCATTTAA
Length of connected seq. 428
Full length Seq ID -
Full length Seq. -
Length of full length seq. -