NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSD442
Library VS
(Link to library)
Clone ID VSD442
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16169-1
Original site URL
Representative seq. ID VSD442E
(Link to Original site)
Representative DNA sequence 
>VSD442 (VSD442Q) /CSM/VS/VSD4-B/VSD442Q.Seq.d/
ATCAATTTAAAAAATGATGAAATTTTTAATTGTTTTAATTTTAGCATTATTCGCTTTATC
AAGTGTTAATGCAAGCAGCGCTATTGAATGCGAACTTTGTGAATATGCTGCCAATTATGG
TGAATTGCTCATTCAATCAAATGCCACCGAAACTGAAATTATCGATAAAGTTGAAAACTT
TTGCAAAGTTATCCCATCTAGTTTCCAAGCCACTTGTGATAGTTTAATCGCAAACTATGG
TAAACAACTCATTCAAATGATTGTCAACAAAGAATCACCAAGCACTTTATGCGCTCAAAT
TGATATGTGTGCATCATCAACTGAAGCCGTCCAAGGTATTTTAGAATGTGACATTTGCCA
ATTCATCGTTAAACAAGTCAACAAATATATTTCAGGTAACGCAACTGAAGCCCAAATCCT
CAAATTCTTAGATACTGATTGTGAAGTCTTTGGTAAAGGTGGTTCAGTAACTTGTCAAAA
CATTGTCAACAACTATGCCCCACAAATCATCAATTTAATCATTAACAACGCATCACCATC
CCAAGTCTGCGGTCTTGTCGGTTTATGTGGCTCCTCTTTGAAAATCGAAGAACCAGTCCA
AGGTGAATTAGAATGTGGTGTTGTGAAGTCATTGCTCAACAATGCTCAAACTACATCAAA
GCCAACAAANCCGAATCTGAAATCGTTGGTCTCCTCGATCAATTCTGTTCAACTCTTTCA
ATCTTTGAAAGTGCTTGTGATACCATCGTTGCCAGCTCTGCTCCAAAGATCATCAACTTA
CTCTTACAAAACCAAAGTGCANCTGTAGTTTGTACCGAAATTGGCTTCTGTGGTAACTCT
TCATCAGGTAATNACAACCATANATCATCTGAAGAATCTTCATCATCTTCCCAACCATCA
TCATCNTCTGAATCATCAGAATCAAGTTACTCNGGTTCAATGACTGGTGCTTCATCNGGT
TCAGCTTCAGGTTCAGGTTCAGGTACATCGGAAACATTAANATTTACATCAAATAAATTT
AAAATTAAA
sequence update 2001. 3.25
Translated Amino Acid sequence 
si*KMMKFLIVLILALFALSSVNASSAIECELCEYAANYGELLIQSNATETEIIDKVENF
CKVIPSSFQATCDSLIANYGKQLIQMIVNKESPSTLCAQIDMCASSTEAVQGILECDICQ
FIVKQVNKYISGNATEAQILKFLDTDCEVFGKGGSVTCQNIVNNYAPQIINLIINNASPS
QVCGLVGLCGSSLKIEEPVQGELECGVVKSLLNNAQTTSKPTXPNLKSLVSSINSVQLFQ
SLKVLVIPSLPALLQRSSTYSYKTKVXL*fvpklasvvtlhqvxttixhlknlhhlpnhh
hxlnhqnqvtxvq*lvlhxvqlqvqvqvhrkh*xlhqinlkl
Translated Amino Acid sequence (All Frames) 
Frame A:
inlkndeifncfnfsiirfikc*ckqry*mrtl*iccqlw*iahsikchrn*nyr*s*kl
lqsypi*fpshl**fnrklw*tthsndcqqritkhfmrsn*yvciin*srpryfrm*hlp
ihr*tsqqiyfr*rn*spnpqilry*l*slw*rwfsnlskhcqqlcptnhqfnh*qriti
pslrscrfmwllfenrrtspr*irmwcceviaqqcsnyikankxeseivglldqfcstls
ifesacdtivassapkiinlllqnqsaxvvcteigfcgnsssgnxnhxsseesssssqps
sssessessysgsmtgassgsasgsgsgtsetlxftsnkfkik


Frame B:
si*KMMKFLIVLILALFALSSVNASSAIECELCEYAANYGELLIQSNATETEIIDKVENF
CKVIPSSFQATCDSLIANYGKQLIQMIVNKESPSTLCAQIDMCASSTEAVQGILECDICQ
FIVKQVNKYISGNATEAQILKFLDTDCEVFGKGGSVTCQNIVNNYAPQIINLIINNASPS
QVCGLVGLCGSSLKIEEPVQGELECGVVKSLLNNAQTTSKPTXPNLKSLVSSINSVQLFQ
SLKVLVIPSLPALLQRSSTYSYKTKVXL*fvpklasvvtlhqvxttixhlknlhhlpnhh
hxlnhqnqvtxvq*lvlhxvqlqvqvqvhrkh*xlhqinlkl


Frame C:
qfkk**nf*lf*f*hyslyqvlmqaallnanfvnmlpimvncsfnqmppklklsiklktf
aklshlvskplviv*sqtmvnnsfk*lstknhqalyalklicvhhqlkpskvf*nvtfan
sslnkstnifqvtqlkpkssns*ilivkslvkvvq*lvktlsttmphkssi*sltthhhp
ksavlsvyvapl*ksknqskvn*nvvl*shcstmlklhqsqqxri*nrwsprsilfnsfn
l*kcl*yhrcqlcskdhqltltkpkcxcslyrnwllw*lfir*xqpxii*rifiifptii
ix*iirikllxfndwcfixfsfrfrfryignixiyik*i*n*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSD442 (VSD442Q) /CSM/VS/VSD4-B/VSD442Q.Seq.d/                       1691   0.0
VFL608 (VFL608Q) /CSM/VF/VFL6-A/VFL608Q.Seq.d/                       1675   0.0
FCL-AB23 (FCL-AB23Q) /CSM/FCL/FCL-AB/FCL-AB23Q.Seq.d/                1675   0.0
VFL571 (VFL571Q) /CSM/VF/VFL5-C/VFL571Q.Seq.d/                       1663   0.0
VSD810 (VSD810Q) /CSM/VS/VSD8-A/VSD810Q.Seq.d/                       1657   0.0
VSJ537 (VSJ537Q) /CSM/VS/VSJ5-B/VSJ537Q.Seq.d/                       1653   0.0
VSG309 (VSG309Q) /CSM/VS/VSG3-A/VSG309Q.Seq.d/                       1641   0.0
SLE371 (SLE371Q) /CSM/SL/SLE3-C/SLE371Q.Seq.d/                       1608   0.0
VFF843 (VFF843Q) /CSM/VF/VFF8-B/VFF843Q.Seq.d/                       1536   0.0
VFF888 (VFF888Q) /CSM/VF/VFF8-D/VFF888Q.Seq.d/                       1176   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AU206530|AU206530.1 Caenorhabditis elegans cDNA clone:yk877f01 : 5' end, single read.   230  3e-56  1
AF175768|AF175768.1 Dictyostelium discoideum SAPLIP C protein mRNA, complete cds.    84  6e-13  2
AC141199|AC141199.1 Rattus norvegicus clone CH230-396G8, WORKING DRAFT SEQUENCE, 25 unordered pieces.    38  0.17  3
AL161623|AL161623.12 Human DNA sequence *** SEQUENCING IN PROGRESS *** from clone  RP11-1K21.    46  0.26  2
AC014621|AC014621.1 Drosophila melanogaster, WORKING DRAFT SEQUENCE, in ordered pieces.    36  0.28  3
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    46  0.30  9
AL157884|AL157884.9 Human DNA sequence from clone RP11-462B18 on chromosome 9p13.1-21.1.    46  0.33  3
AP004126|AP004126.2 Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 2, BAC clone:OJ9003_G05, WORKING DRAFT SEQUENCE, 1 ordered pieces.    38  0.84  2
AL425141|AL425141.1 clone BA0AB003E10 of library BA0AB from strain CLIB 210 of  Kluyveromyces lactis.    46  0.86  1
AF267217|AF267217.1 Candidatus Carsonella ruddii natural-host Pachypsylla celtidis ATP synthase alpha subunit (atpA) gene, partial cds; ATP synthase gamma subunit (atpG) gene, complete cds; and ATP synthase beta subunit (atpD) gene, partial cds.    46  0.86  1
dna update 2003. 9.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U66367_1(U66367|pid:none) Dictyostelium discoideum SapA (sapA) m...   105   6e-27
BT045327_1(BT045327|pid:none) Salmo salar clone ssal-rgf-518-210...   107   5e-22
BT058972_1(BT058972|pid:none) Salmo salar clone ssal-rgf-517-167...   107   5e-22
(O13035) RecName: Full=Proactivator polypeptide; Contains:   Rec...   107   7e-22
AF108656_1(AF108656|pid:none) Gallus gallus prosaposin mRNA, com...   106   2e-21
BT045905_1(BT045905|pid:none) Salmo salar clone ssal-rgf-536-147...   106   2e-21
CR388420_1(CR388420|pid:none) Zebrafish DNA sequence from clone ...   100   6e-20
BC056717_1(BC056717|pid:none) Danio rerio prosaposin, mRNA (cDNA...   100   6e-20
AF276996_1(AF276996|pid:none) Danio rerio lysosomal cofactor/neu...   100   6e-20
AB209776_1(AB209776|pid:none) Homo sapiens mRNA for prosaposin v...    99   2e-19
protein update 2009. 7.29
PSORT 

 psg: 0.99  gvh: 0.88  alm: 0.34  top: 0.07  tms: 0.00  mit: 0.35  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  4.0 %: mitochondrial
	  4.0 %: nuclear

>> prediction for VSD442 is exc
5' end seq. ID VSD442F
5' end seq. 
>VSD442F.Seq
ATCAATTTAAAAAATGATGAAATTTTTAATTGTTTTAATTTTAGCATTATTCGCTTTATC
AAGTGTTAATGCAAGCAGCGCTATTGAATGCGAACTTTGTGAATATGCTGCCAATTATGG
TGAATTGCTCATTCAATCAAATGCCACCGAAACTGAAATTATCGATAAAGTTGAAAACTT
TTGCAAAGTTATCCCATCTAGTTTCCAAGCCACTTGTGATAGTTTAATCGCAAACTATGG
TAAACAACTCATTCAAATGATTGTCAACAAAGAATCACCAAGCACTTTATGCGCTCAAAT
TGATATGTGTGCATCATCAACTGAAGCCGTCCAAGGTATTTTAGAATGTGACATTTGCCA
ATTCATCGTTAAACAAGTCAACAAATATATTTCAGGTAACGCAACTGAAGCCCAAATCCT
CAAATTCTTAGATACTGATTGTGAAGTCTTTGGTAAAGGTGGTTCAGTAACTTGTCAAAA
CATTGTCAACAACTATGCCCCACAAATCATCAATTTAATCATTAACAACGCATCACCATC
CCAAGTCTGCGGTCTTGTCGGTTTATGTGGCT----------
Length of 5' end seq. 572
3' end seq. ID VSD442Z
3' end seq. 
>VSD442Z.Seq
----------CTCNAATTCTTAGATACTGATTGTGAAGTCTTTGGTAAAGGTGGTTCAGT
AACTTGTCAAAACATTGTCAACAACTATGCCCCACAAATCATCAATTTAATCATTAACAA
CGCATCACCATCCCAAGTNTGCGGTCTTGTCGGTTTATGTGGCTCCTCTTTGAAAATCGA
AGAACCAGTCCAAGGTGAATTAGAATGTGGTGTTGTGAAGTCATTGCTCAACAATGCTCA
AACTACATCAAAGCCAACAAANCCGAATCTGAAATCGTTGGTCTCCTCGATCAATTCTGT
TCAACTCTTTCAATCTTTGAAAGTGCTTGTGATACCATCGTTGCCAGCTCTGCTCCAAAG
ATCATCAACTTACTCTTACAAAACCAAAGTGCANCTGTAGTTTGTACCGAAATTGGCTTC
TGTGGTAACTCTTCATCAGGTAATNACAACCATANATCATCTGAAGAATCTTCATCATCT
TCCCAACCATCATCATCNTCTGAATCATCAGAATCAAGTTACTCNGGTTCAATGACTGGT
GCTTCATCNGGTTCAGCTTCAGGTTCAGGTTCAGGTACATCGGAAACATTAANATTTACA
TCAAATAAATTTAAAATTAAA
Length of 3' end seq. 611
Connected seq. ID VSD442P
Connected seq. 
>VSD442P.Seq
ATCAATTTAAAAAATGATGAAATTTTTAATTGTTTTAATTTTAGCATTATTCGCTTTATC
AAGTGTTAATGCAAGCAGCGCTATTGAATGCGAACTTTGTGAATATGCTGCCAATTATGG
TGAATTGCTCATTCAATCAAATGCCACCGAAACTGAAATTATCGATAAAGTTGAAAACTT
TTGCAAAGTTATCCCATCTAGTTTCCAAGCCACTTGTGATAGTTTAATCGCAAACTATGG
TAAACAACTCATTCAAATGATTGTCAACAAAGAATCACCAAGCACTTTATGCGCTCAAAT
TGATATGTGTGCATCATCAACTGAAGCCGTCCAAGGTATTTTAGAATGTGACATTTGCCA
ATTCATCGTTAAACAAGTCAACAAATATATTTCAGGTAACGCAACTGAAGCCCAAATCCT
CAAATTCTTAGATACTGATTGTGAAGTCTTTGGTAAAGGTGGTTCAGTAACTTGTCAAAA
CATTGTCAACAACTATGCCCCACAAATCATCAATTTAATCATTAACAACGCATCACCATC
CCAAGTCTGCGGTCTTGTCGGTTTATGTGGCT----------CTCNAATTCTTAGATACT
GATTGTGAAGTCTTTGGTAAAGGTGGTTCAGTAACTTGTCAAAACATTGTCAACAACTAT
GCCCCACAAATCATCAATTTAATCATTAACAACGCATCACCATCCCAAGTNTGCGGTCTT
GTCGGTTTATGTGGCTCCTCTTTGAAAATCGAAGAACCAGTCCAAGGTGAATTAGAATGT
GGTGTTGTGAAGTCATTGCTCAACAATGCTCAAACTACATCAAAGCCAACAAANCCGAAT
CTGAAATCGTTGGTCTCCTCGATCAATTCTGTTCAACTCTTTCAATCTTTGAAAGTGCTT
GTGATACCATCGTTGCCAGCTCTGCTCCAAAGATCATCAACTTACTCTTACAAAACCAAA
GTGCANCTGTAGTTTGTACCGAAATTGGCTTCTGTGGTAACTCTTCATCAGGTAATNACA
ACCATANATCATCTGAAGAATCTTCATCATCTTCCCAACCATCATCATCNTCTGAATCAT
CAGAATCAAGTTACTCNGGTTCAATGACTGGTGCTTCATCNGGTTCAGCTTCAGGTTCAG
GTTCAGGTACATCGGAAACATTAANATTTACATCAAATAAATTTAAAATTAAA
Length of connected seq. 1183
Full length Seq ID VSD442E
Full length Seq. 
>VSD442E.Seq
ATCAATTTAAAAAATGATGAAATTTTTAATTGTTTTAATTTTAGCATTATTCGCTTTATC
AAGTGTTAATGCAAGCAGCGCTATTGAATGCGAACTTTGTGAATATGCTGCCAATTATGG
TGAATTGCTCATTCAATCAAATGCCACCGAAACTGAAATTATCGATAAAGTTGAAAACTT
TTGCAAAGTTATCCCATCTAGTTTCCAAGCCACTTGTGATAGTTTAATCGCAAACTATGG
TAAACAACTCATTCAAATGATTGTCAACAAAGAATCACCAAGCACTTTATGCGCTCAAAT
TGATATGTGTGCATCATCAACTGAAGCCGTCCAAGGTATTTTAGAATGTGACATTTGCCA
ATTCATCGTTAAACAAGTCAACAAATATATTTCAGGTAACGCAACTGAAGCCCAAATCCT
CAAATTCTTAGATACTGATTGTGAAGTCTTTGGTAAAGGTGGTTCAGTAACTTGTCAAAA
CATTGTCAACAACTATGCCCCACAAATCATCAATTTAATCATTAACAACGCATCACCATC
CCAAGTCTGCGGTCTTGTCGGTTTATGTGGCTCCTCTTTGAAAATCGAAGAACCAGTCCA
AGGTGAATTAGAATGTGGTGTTGTGAAGTCATTGCTCAACAATGCTCAAACTACATCAAA
GCCAACAAANCCGAATCTGAAATCGTTGGTCTCCTCGATCAATTCTGTTCAACTCTTTCA
ATCTTTGAAAGTGCTTGTGATACCATCGTTGCCAGCTCTGCTCCAAAGATCATCAACTTA
CTCTTACAAAACCAAAGTGCANCTGTAGTTTGTACCGAAATTGGCTTCTGTGGTAACTCT
TCATCAGGTAATNACAACCATANATCATCTGAAGAATCTTCATCATCTTCCCAACCATCA
TCATCNTCTGAATCATCAGAATCAAGTTACTCNGGTTCAATGACTGGTGCTTCATCNGGT
TCAGCTTCAGGTTCAGGTTCAGGTACATCGGAAACATTAANATTTACATCAAATAAATTT
AAAATTAAA
Length of full length seq. 1029