>VSD357 (VSD357Q) /CSM/VS/VSD3-C/VSD357Q.Seq.d/
AATTTAGCGAAAATCAACAAATACATTTCAAAATAATAAAAATAAAAAAAATGAGTGGAT
ACGTTGGAAACCTTTCACCAGAACAAGAACATCAATTAGGTATTATTAAATCTAAAATTG
CAGAAATTAATGATCCAGTTGTAGAAAAAGAAATTCAACAATTAGATGATTCTATGACCT
TAAGATTTTTAAGAGCTAGAAAATGGAATCTAAAAGATTCATTTGATATGCTCTATGAAG
CATTAAAATTCCGAGCAACATTTCAAGATGTTGGAGTTGAAGGTATAACAGAATCAATGG
TTGTTAATGAATTAAAGAGTGGTAAGAGTTATTTCCATGGTGTTGATAAAGGTGGTAGAC
CAGTTTGTATTGTTAAAACCAGTAGACATGATAGTTACAACAGAGATTTGAATGAATCGA
TGAGATATTGTGTCTACGTAATGGAAAATGGTAAATCAATGTTAAAGGACGGTATTGAAA
CTTGTACACTTATTTTCGATATGTCTGATTTTAGTTCAAAAAATATGGATTATCCATTGG
TTAAATTTATGGTTGAATTATTTCAAAAGTTTTATCCAGAATCATTACAAAAATGTTTAA
TTTTAAATGCACCTTGGATCTTTATGGGAATTTGGCATATCATCAAACATTGGTTAGATC
CAAATACCGCTTCAAAAGTTTCTTTTGTCAAAACTAAACAATTGGTTGATTATATTCCAA
AAGATCAATTAGAAAGTTCTTATGGNGAACAAGTACATTTAAATATACTTANANGAAAAG
AA

FSENQQIHFKIIKIKKMSGYVGNLSPEQEHQLGIIKSKIAEINDPVVEKEIQQLDDSMTL
RFLRARKWNLKDSFDMLYEALKFRATFQDVGVEGITESMVVNELKSGKSYFHGVDKGGRP
VCIVKTSRHDSYNRDLNESMRYCVYVMENGKSMLKDGIETCTLIFDMSDFSSKNMDYPLV
KFMVELFQKFYPESLQKCLILNAPWIFMGIWHIIKHWLDPNTASKVSFVKTKQLVDYIPK
DQLESSYGEQVHLNILXXKE

Frame A:
nlakinkyisk**k*kk*vdtletfhqnknin*vllnlklqklmiql*kkkfnn*mil*p
*df*elengi*kihlicsmkh*nseqhfkmlelkv*qnqwllmn*rvvrvismvlikvvd
qfvllkpvdmivttei*mnr*divst*wkmvnqc*rtvlklvhlfsiclilvqkiwiihw
lnlwlnyfksfiqnhyknv*f*mhlgslwefgissnig*iqiplqkfllsklnnwliifq
kin*kvlmxnkyi*iylxek


Frame B:
i*rkstntfqnnknkknewirwkpftrtrtsiryy*i*ncrn**sscrkrnstir*fydl
kifks*kmeskrfi*yal*sikipsnisrcws*rynringc**ikew*elfpwc**rw*t
slyc*nq*t**lqqrfe*ideilclrngkw*invkgry*nlytyfryv*f*fkkyglsig
*iyg*iiskvlsriitkmfnfkctldlygnlayhqtlvrskyrfksffcqn*tig*lysk
rsirkflwxtstfkytxxkr


Frame C:
FSENQQIHFKIIKIKKMSGYVGNLSPEQEHQLGIIKSKIAEINDPVVEKEIQQLDDSMTL
RFLRARKWNLKDSFDMLYEALKFRATFQDVGVEGITESMVVNELKSGKSYFHGVDKGGRP
VCIVKTSRHDSYNRDLNESMRYCVYVMENGKSMLKDGIETCTLIFDMSDFSSKNMDYPLV
KFMVELFQKFYPESLQKCLILNAPWIFMGIWHIIKHWLDPNTASKVSFVKTKQLVDYIPK
DQLESSYGEQVHLNILXXKE

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSD357 (VSD357Q) /CSM/VS/VSD3-C/VSD357Q.Seq.d/                       1431   0.0
SSE832 (SSE832Q) /CSM/SS/SSE8-B/SSE832Q.Seq.d/                       1306   0.0
SSB584 (SSB584Q) /CSM/SS/SSB5-D/SSB584Q.Seq.d/                        890   0.0
FC-IC0515 (FC-IC0515Q) /CSM/FC-IC/FC-IC0515Q.Seq.d/                   515   e-145
FC-IC0335 (FC-IC0335Q) /CSM/FC-IC/FC-IC0335Q.Seq.d/                   515   e-145
SSJ189 (SSJ189Q) /CSM/SS/SSJ1-D/SSJ189Q.Seq.d/                        349   4e-95
VSH630 (VSH630Q) /CSM/VS/VSH6-B/VSH630Q.Seq.d/                         40   0.050
VHH823 (VHH823Q) /CSM/VH/VHH8-A/VHH823Q.Seq.d/                         40   0.050
VFI881 (VFI881Q) /CSM/VF/VFI8-D/VFI881Q.Seq.d/                         40   0.050
CFF674 (CFF674Q) /CSM/CF/CFF6-D/CFF674Q.Seq.d/                         40   0.050

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC117082|AC117082.2 Dictyostelium discoideum chromosome 2 map complement(6807709-6776860) strain AX4, complete sequence.  1116  0.0  4
AC073573|AC073573.27 Homo sapiens 12 BAC RP11-1136G11 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    40  0.51  2
AC025133|AC025133.6 Homo sapiens chromosome 8, clone RP11-227F6, complete sequence.    36  0.52  5
AP002009|AP002009.2 Homo sapiens genomic DNA, chromosome 11q13 clone:RP11-857A13, WORKING DRAFT SEQUENCE, 28 unordered pieces.    46  0.65  1
AC015677|AC015677.4 Homo sapiens chromosome 18 clone RP11-205G20 map 18, WORKING DRAFT SEQUENCE, 20 unordered pieces.    46  0.65  1
AP001283|AP001283.2 Homo sapiens genomic DNA, chromosome 18p11.2 clone:RP11-820A8, WORKING DRAFT SEQUENCE, 27 unordered pieces.    46  0.65  1
BU656121|BU656121.1 cl06g02.z1 Hembase; Erythroid Precursor Cells (LCB:cl library) Homo sapiens cDNA clone cl06g02 5', mRNA sequence.    46  0.65  1
AC100954|AC100954.1 Mus musculus clone RP23-73P20, LOW-PASS SEQUENCE SAMPLING.    46  0.65  1
BU656042|BU656042.1 cl05g02.z1 Hembase; Erythroid Precursor Cells (LCB:cl library) Homo sapiens cDNA clone cl05g02 5', mRNA sequence.    46  0.65  1
BX324138|BX324138.2 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-127F10.    46  0.65  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270115_50(AM270115|pid:none) Aspergillus niger contig An06c009...   165   2e-39
(Q10138) RecName: Full=CRAL-TRIO domain-containing protein C3H8....   156   8e-37
CR382129_654(CR382129|pid:none) Yarrowia lipolytica strain CLIB1...   145   1e-33
AM920431_638(AM920431|pid:none) Penicillium chrysogenum Wisconsi...   144   3e-33
CR382125_231(CR382125|pid:none) Kluyveromyces lactis strain NRRL...   141   2e-32
CR382129_199(CR382129|pid:none) Yarrowia lipolytica strain CLIB1...   140   5e-32
AM269993_6(AM269993|pid:none) Aspergillus niger contig An01c0470...   139   1e-31
CU928168_586(CU928168|pid:none) Kluyveromyces thermotolerans str...   137   4e-31
CR382133_199(CR382133|pid:none) Debaryomyces hansenii strain CBS...   132   1e-29
CU928174_505(CU928174|pid:none) Zygosaccharomyces rouxii strain ...   127   4e-28

 psg: 0.62  gvh: 0.31  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for VSD357 is cyt

>VSD357F.Seq
AATTTAGCGAAAATCAACAAATACATTTCAAAATAATAAAAATAAAAAAAATGAGTGGAT
ACGTTGGAAACCTTTCACCAGAACAAGAACATCAATTAGGTATTATTAAATCTAAAATTG
CAGAAATTAATGATCCAGTTGTAGAAAAAGAAATTCAACAATTAGATGATTCTATGACCT
TAAGATTTTTAAGAGCTAGAAAATGGAATCTAAAAGATTCATTTGATATGCTCTATGAAG
CATTAAAATTCCGAGCAACATTTCAAGATGTTGGAGTTGAAGGTATAACAGAATCAATGG
TTGTTAATGAATTAAAGAGTGGTAAGAGTTATTTCCATGGTGTTGATAAAGGTGGTAGAC
CAGTTTGTATTGTTAAAACCAGTAGACATGATAGTTACAACAGAGATTTGAATGAATCGA
TGAGATATTGTGTCTACGTAATGGAAAATGGTAAATCAATGTTAAAGGACGGTATTGAAA
CTTGTACACTTATTTTCGATATGTCTGATTTTAGTTCAAAAAATATGGATTATCCA----
------

>VSD357Z.Seq
----------ACCTTTCACCAGAACAAGAACATCAATTAGGTATTATTAAATCTAAAATT
GCAGAAATTAATGATCCAGTTGTAGAAAAAGAAATTCAACAATTAGATGATTCTATGACC
TTAAGATTTTTAAGAGCTAGAAAATGGAATCTAAAAGATTCATTTGATATGCTCTATGAA
GCATTAAAATTCCGAGCAACATTTCAAGATGTTGGAGTTGAAGGTATAACAGAATCAATG
GTTGTTAATGAATTAAAGAGTGGTAAGAGTTATTTCCATGGTGTTGATAAAGGTGGTAGA
CCAGTTTGTATTGTTAAAACCAGTAGACATGATAGTTACAACAGAGATTTGAATGAATCG
ATGAGATATTGTGTCTACGTAATGGAAAATGGTAAATCAATGTTAAAGGACGGTATTGAA
ACTTGTACACTTATTTTCGATATGTCTGATTTTAGTTCAAAAAATATGGATTATCCATTG
GTTAAATTTATGGTTGAATTATTTCAAAAGTTTTATCCAGAATCATTACAAAAATGTTTA
ATTTTAAATGCACCTTGGATCTTTATGGGAATTTGGCATATCATCAAACATTGGTTAGAT
CCAAATACCGCTTCAAAAGTTTCTTTTGTCAAAACTAAACAATTGGTTGATTATATTCCA
AAAGATCAATTAGAAAGTTCTTATGGNGAACAAGTACATTTAAATATACTTANANGAAAA
GAA

>VSD357P.Seq
AATTTAGCGAAAATCAACAAATACATTTCAAAATAATAAAAATAAAAAAAATGAGTGGAT
ACGTTGGAAACCTTTCACCAGAACAAGAACATCAATTAGGTATTATTAAATCTAAAATTG
CAGAAATTAATGATCCAGTTGTAGAAAAAGAAATTCAACAATTAGATGATTCTATGACCT
TAAGATTTTTAAGAGCTAGAAAATGGAATCTAAAAGATTCATTTGATATGCTCTATGAAG
CATTAAAATTCCGAGCAACATTTCAAGATGTTGGAGTTGAAGGTATAACAGAATCAATGG
TTGTTAATGAATTAAAGAGTGGTAAGAGTTATTTCCATGGTGTTGATAAAGGTGGTAGAC
CAGTTTGTATTGTTAAAACCAGTAGACATGATAGTTACAACAGAGATTTGAATGAATCGA
TGAGATATTGTGTCTACGTAATGGAAAATGGTAAATCAATGTTAAAGGACGGTATTGAAA
CTTGTACACTTATTTTCGATATGTCTGATTTTAGTTCAAAAAATATGGATTATCCA----
------ACCTTTCACCAGAACAAGAACATCAATTAGGTATTATTAAATCTAAAATTGCAG
AAATTAATGATCCAGTTGTAGAAAAAGAAATTCAACAATTAGATGATTCTATGACCTTAA
GATTTTTAAGAGCTAGAAAATGGAATCTAAAAGATTCATTTGATATGCTCTATGAAGCAT
TAAAATTCCGAGCAACATTTCAAGATGTTGGAGTTGAAGGTATAACAGAATCAATGGTTG
TTAATGAATTAAAGAGTGGTAAGAGTTATTTCCATGGTGTTGATAAAGGTGGTAGACCAG
TTTGTATTGTTAAAACCAGTAGACATGATAGTTACAACAGAGATTTGAATGAATCGATGA
GATATTGTGTCTACGTAATGGAAAATGGTAAATCAATGTTAAAGGACGGTATTGAAACTT
GTACACTTATTTTCGATATGTCTGATTTTAGTTCAAAAAATATGGATTATCCATTGGTTA
AATTTATGGTTGAATTATTTCAAAAGTTTTATCCAGAATCATTACAAAAATGTTTAATTT
TAAATGCACCTTGGATCTTTATGGGAATTTGGCATATCATCAAACATTGGTTAGATCCAA
ATACCGCTTCAAAAGTTTCTTTTGTCAAAACTAAACAATTGGTTGATTATATTCCAAAAG
ATCAATTAGAAAGTTCTTATGGNGAACAAGTACATTTAAATATACTTANANGAAAAGAA

>VSD357E.Seq
AATTTAGCGAAAATCAACAAATACATTTCAAAATAATAAAAATAAAAAAAATGAGTGGAT
ACGTTGGAAACCTTTCACCAGAACAAGAACATCAATTAGGTATTATTAAATCTAAAATTG
CAGAAATTAATGATCCAGTTGTAGAAAAAGAAATTCAACAATTAGATGATTCTATGACCT
TAAGATTTTTAAGAGCTAGAAAATGGAATCTAAAAGATTCATTTGATATGCTCTATGAAG
CATTAAAATTCCGAGCAACATTTCAAGATGTTGGAGTTGAAGGTATAACAGAATCAATGG
TTGTTAATGAATTAAAGAGTGGTAAGAGTTATTTCCATGGTGTTGATAAAGGTGGTAGAC
CAGTTTGTATTGTTAAAACCAGTAGACATGATAGTTACAACAGAGATTTGAATGAATCGA
TGAGATATTGTGTCTACGTAATGGAAAATGGTAAATCAATGTTAAAGGACGGTATTGAAA
CTTGTACACTTATTTTCGATATGTCTGATTTTAGTTCAAAAAATATGGATTATCCATTGG
TTAAATTTATGGTTGAATTATTTCAAAAGTTTTATCCAGAATCATTACAAAAATGTTTAA
TTTTAAATGCACCTTGGATCTTTATGGGAATTTGGCATATCATCAAACATTGGTTAGATC
CAAATACCGCTTCAAAAGTTTCTTTTGTCAAAACTAAACAATTGGTTGATTATATTCCAA
AAGATCAATTAGAAAGTTCTTATGGNGAACAAGTACATTTAAATATACTTANANGAAAAG
AA