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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSD320
Library VS
(Link to library)
Clone ID VSD320
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14349-1
Original site URL
Representative seq. ID VSD320P
(Link to Original site)
Representative DNA sequence 
>VSD320 (VSD320Q) /CSM/VS/VSD3-A/VSD320Q.Seq.d/
AAAAAATGTCTTCAGGTATTGCTTTAGCTCCAAACTGCGTTTCAACCTTCAACGATTTAA
AGTTAGGTCGTAAATATGGTGGTATCATCTACAGAATCTCTGACGATTCAAAAGAAATCA
TTGTTGACTCCACCTTACCAGCTGGTTGTTCATTCGATGAATTCACCAAATGTCTCCCAG
AAAACGAATGTAGATACGTTGTCCTCGACTATCAATACAAAGAAGAAGGTGCCCAAAAGA
GCAAAATCTGTTTTGTTGCCTGGTGCCCAGATACCGCCAACATTAAAAAGAAAATGATGG
CCACCTCATCAAAAGACTCTTTACGTAAAGCCTGTGTTGGTATCCAAGTTGAAATCCAAG
GTACCGATGCCTCTGAAGTCAAAGATTCATGTTTCTATGAAAAATGTACCAAAATCTAAA
TAATTTGAAAAAATTTAAAAAAAAAAXXXXXXXXXXAAAAAATGTCTTCAGGTATTGCTT
TAGCTCCAAACTGCGTTTCAACCTTCAACGATTTAAAGTTAGGTCGTAAATATGGTGGTA
TCATCTACAGAATCTCTGACGATTCAAAAGAAATCATTGTTGACTCCACCTTACCAGCTG
GTTGTTCATTCGATGAATTCACCAAATGTCTCCCAGAAAACGAATGTAGATACGTTGTCC
TCGACTATCAATACAAAGAAGAAGGTGCCCAAAAGAGCAAAATCTGTTTTGTTGCCTGGT
GCCCAGATACCGCCAACATTAAAAAGAAAATGATGGCCACCTCATCAAAAGACTCTTTAC
GTAAAGCCTGTGTTGGTA
sequence update 2001. 3.22
Translated Amino Acid sequence 
knvfrycfssklrfnlqrfkvrs*iwwyhlqnl*rfkrnhc*lhltswlfir*ihqmspr
krm*ircprlsiqrrrcpkeqnlfcclvpryrqh*kendghlikrlft*slcwyps*npr
yrcl*sqrfmfl*KMYQNLNNLKKFKKK---

---KMSSGIALAPNCVSTFNDLKLGRKYGGIIYRISDDSKEIIVDSTLPAGCSFDEFTKC
LPENECRYVVLDYQYKEEGAQKSKICFVAWCPDTANIKKKMMATSSKDSLRKACVG
Translated Amino Acid sequence (All Frames) 
Frame A:
kkclqvll*lqtafqpsti*s*vvnmvvsstesltiqkkslltppyqlvvhsmnspnvsq
ktnvdtlsstintkkkvpkraksvllpgaqipptlkrk*wpphqktlyvkpvlvsklksk
vpmplkskihvsmknvpksk*feki*kk---

---kkclqvll*lqtafqpsti*s*vvnmvvsstesltiqkkslltppyqlvvhsmnspn
vsqktnvdtlsstintkkkvpkraksvllpgaqipptlkrk*wpphqktlyvkpvlv

Frame B:
knvfrycfssklrfnlqrfkvrs*iwwyhlqnl*rfkrnhc*lhltswlfir*ihqmspr
krm*ircprlsiqrrrcpkeqnlfcclvpryrqh*kendghlikrlft*slcwyps*npr
yrcl*sqrfmfl*KMYQNLNNLKKFKKK---

---knvfrycfssklrfnlqrfkvrs*iwwyhlqnl*rfkrnhc*lhltswlfir*ihqm
sprkrm*ircprlsiqrrrcpkeqnlfcclvpryrqh*kendghlikrlft*slcw

Frame C:
kmssgialapncvstfndlklgrkyggiiyrisddskeiivdstlpagcsfdeftkclpe
necryvvldyqykeegaqkskicfvawcpdtanikkkmmatsskdslrkacvgiqveiqg
tdasevkdscfyekctki*ii*knlkkk---

---KMSSGIALAPNCVSTFNDLKLGRKYGGIIYRISDDSKEIIVDSTLPAGCSFDEFTKC
LPENECRYVVLDYQYKEEGAQKSKICFVAWCPDTANIKKKMMATSSKDSLRKACVG
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ890 (VSJ890Q) /CSM/VS/VSJ8-D/VSJ890Q.Seq.d/                        846   0.0
VSJ584 (VSJ584Q) /CSM/VS/VSJ5-D/VSJ584Q.Seq.d/                        846   0.0
VSJ550 (VSJ550Q) /CSM/VS/VSJ5-C/VSJ550Q.Seq.d/                        846   0.0
VSJ437 (VSJ437Q) /CSM/VS/VSJ4-B/VSJ437Q.Seq.d/                        846   0.0
VSJ243 (VSJ243Q) /CSM/VS/VSJ2-B/VSJ243Q.Seq.d/                        846   0.0
VSI135 (VSI135Q) /CSM/VS/VSI1-B/VSI135Q.Seq.d/                        846   0.0
VSG665 (VSG665Q) /CSM/VS/VSG6-C/VSG665Q.Seq.d/                        846   0.0
VSD383 (VSD383Q) /CSM/VS/VSD3-D/VSD383Q.Seq.d/                        846   0.0
VSD320 (VSD320Q) /CSM/VS/VSD3-A/VSD320Q.Seq.d/                        846   0.0
VFL333 (VFL333Q) /CSM/VF/VFL3-B/VFL333Q.Seq.d/                        846   0.0
own update 2001.11.29
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

D37981|D37981.1 Dictyostelium discoidium DDCOF2 gene for cofilin, complete cds.   846  0.0  1
D37980|D37980.1 Dictyostelium discoidium DDCOF1 gene for cofilin, complete cds (exon1-2).   833  0.0  1
X04004|X04004.1 Dictyostelium discoideum mRNA for contact site A (csA) protein.   168  7e-38  1
AC143994|AC143994.1 Macaca mulatta clone CH250-272N23, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    38  0.050  3
AW399043|AW399043.1 EST309543 L. pennellii trichome, Cornell University Lycopersicon pennellii cDNA clone cLPT5H2 5', mRNA sequence.    34  0.71  2
AC135132|AC135132.2 Rattus norvegicus clone CH230-240G18, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    44  2.6  1
AC115450|AC115450.3 Rattus norvegicus clone CH230-210N1, *** SEQUENCING IN PROGRESS ***, 7 unordered pieces.    44  2.6  1
AF411059|AF411059.1 Homo sapiens clone BAC_22608 inducible T-cell co-stimulator (ICOS) gene, complete cds; and endogenous virus HERV-H, complete sequence.    44  2.6  3
BZ859527|BZ859527.1 CH240_269E4.TJ CHORI-240 Bos taurus genomic clone CH240_269E4, DNA sequence.    44  2.6  1
CB799241|CB799241.1 AMGNNUC:NRHY4-00139-G2-A W Rat hypothalamus (10464) Rattus norvegicus cDNA clone nrhy4-00139-g2 5', mRNA sequence.    44  2.6  1
dna update 2003. 8.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54706) RecName: Full=Cofilin-1;  &D37980_1(D37980|pid:none) &D...   280   3e-74
(P37167) RecName: Full=Actophorin;  &M93361_1(M93361|pid:none)        130   5e-29
FM992692_38(FM992692|pid:none) Candida dubliniensis CD36 chromos...   125   2e-27
(Q96VU9) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...   125   2e-27
AY428595_1(AY428595|pid:none) Helianthus annuus clone HA10-7 put...   123   6e-27
CP000501_299(CP000501|pid:none) Pichia stipitis CBS 6054 chromos...   120   7e-26
(Q9HF97) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...   119   1e-25
(Q6CQ22) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...   119   1e-25
CU928179_306(CU928179|pid:none) Zygosaccharomyces rouxii strain ...   116   7e-25
(Q03048) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...   116   7e-25
protein update 2009. 7.29
PSORT 

 psg: 0.48  gvh: 0.42  alm: 0.50  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.08
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 16.0 %: nuclear
	  4.0 %: peroxisomal

>> prediction for VSD320 is cyt
5' end seq. ID VSD320F
5' end seq. 
>VSD320F.Seq
AAAAAATGTCTTCAGGTATTGCTTTAGCTCCAAACTGCGTTTCAACCTTCAACGATTTAA
AGTTAGGTCGTAAATATGGTGGTATCATCTACAGAATCTCTGACGATTCAAAAGAAATCA
TTGTTGACTCCACCTTACCAGCTGGTTGTTCATTCGATGAATTCACCAAATGTCTCCCAG
AAAACGAATGTAGATACGTTGTCCTCGACTATCAATACAAAGAAGAAGGTGCCCAAAAGA
GCAAAATCTGTTTTGTTGCCTGGTGCCCAGATACCGCCAACATTAAAAAGAAAATGATGG
CCACCTCATCAAAAGACTCTTTACGTAAAGCCTGTGTTGGTATCCAAGTTGAAATCCAAG
GTACCGATGCCTCTGAAGTCAAAGATTCATGTTTCTATGAAAAATGTACCAAAATCTAAA
TAATTTGAAAAAATTTAAAAAAAAAA----------
Length of 5' end seq. 446
3' end seq. ID VSD320Z
3' end seq. 
>VSD320Z.Seq
----------AAAAAATGTCTTCAGGTATTGCTTTAGCTCCAAACTGCGTTTCAACCTTC
AACGATTTAAAGTTAGGTCGTAAATATGGTGGTATCATCTACAGAATCTCTGACGATTCA
AAAGAAATCATTGTTGACTCCACCTTACCAGCTGGTTGTTCATTCGATGAATTCACCAAA
TGTCTCCCAGAAAACGAATGTAGATACGTTGTCCTCGACTATCAATACAAAGAAGAAGGT
GCCCAAAAGAGCAAAATCTGTTTTGTTGCCTGGTGCCCAGATACCGCCAACATTAAAAAG
AAAATGATGGCCACCTCATCAAAAGACTCTTTACGTAAAGCCTGTGTTGGTA
Length of 3' end seq. 342
Connected seq. ID VSD320P
Connected seq. 
>VSD320P.Seq
AAAAAATGTCTTCAGGTATTGCTTTAGCTCCAAACTGCGTTTCAACCTTCAACGATTTAA
AGTTAGGTCGTAAATATGGTGGTATCATCTACAGAATCTCTGACGATTCAAAAGAAATCA
TTGTTGACTCCACCTTACCAGCTGGTTGTTCATTCGATGAATTCACCAAATGTCTCCCAG
AAAACGAATGTAGATACGTTGTCCTCGACTATCAATACAAAGAAGAAGGTGCCCAAAAGA
GCAAAATCTGTTTTGTTGCCTGGTGCCCAGATACCGCCAACATTAAAAAGAAAATGATGG
CCACCTCATCAAAAGACTCTTTACGTAAAGCCTGTGTTGGTATCCAAGTTGAAATCCAAG
GTACCGATGCCTCTGAAGTCAAAGATTCATGTTTCTATGAAAAATGTACCAAAATCTAAA
TAATTTGAAAAAATTTAAAAAAAAAA----------AAAAAATGTCTTCAGGTATTGCTT
TAGCTCCAAACTGCGTTTCAACCTTCAACGATTTAAAGTTAGGTCGTAAATATGGTGGTA
TCATCTACAGAATCTCTGACGATTCAAAAGAAATCATTGTTGACTCCACCTTACCAGCTG
GTTGTTCATTCGATGAATTCACCAAATGTCTCCCAGAAAACGAATGTAGATACGTTGTCC
TCGACTATCAATACAAAGAAGAAGGTGCCCAAAAGAGCAAAATCTGTTTTGTTGCCTGGT
GCCCAGATACCGCCAACATTAAAAAGAAAATGATGGCCACCTCATCAAAAGACTCTTTAC
GTAAAGCCTGTGTTGGTA
Length of connected seq. 788
Full length Seq ID -
Full length Seq. -
Length of full length seq. -