NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSD303
Library VS
(Link to library)
Clone ID VSD303
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14139-1
Original site URL
Representative seq. ID VSD303P
(Link to Original site)
Representative DNA sequence 
>VSD303 (VSD303Q) /CSM/VS/VSD3-A/VSD303Q.Seq.d/
AGTTAATGTTCATTACTTTGAAGATGGTAATGTTCAATTAAACACTGTCACTCAAAAACA
AACTACTTCACCATCTGCTGATGCACAATCAACTGCAGTCAATGCATTTAAAGCAATTGG
TAAAGCTGAACTTAACCTCCACACCGCACTTGATAACAATTACTCCACTATGGGTGATAC
CACATTCAAAGCCCTTAGACGTGCTCTTCCAATCAATCGTACCAAAATCAACTGGCAAAA
AGTCAAAAACTTCAAAATTGCCAATGAACTCAATAAATAAATTTAAAACTAATTCACTTC
TTGTTCACCAATTCTATATTAATATCTTGTCACACGTAAAATAAAAAATAAAAAATAAAT
AAAAATAAAATAAATAAAAATAATTATTTATTTATTTACAATTAAAAAAAAAAXXXXXXX
XXXAGTTAATGTTCATTACTTTGAAGATGGTAATGTTCAATTAAACACTGTCACTCAAAA
ACAAACTACTTCACCATCTGCTGATGCACAATCAACTGCAGTCAATGCATTTAAAGCAAT
TGGTAAAGCTGAACTTAACCTCCACACCGCACTTGATAACAATTACTCCACTATGGGTGA
TACCACATTCAAAGCCCTTAGACGTGCTCTTCCAATCAATCGTACCAAAATCAACTGGCA
AAAAGTCAAAAACTTCAAAATTGCCAATGAACTCAATAAATAAATTTAAAACTAATTCAC
TCTTGTTCACCAATTCTATATTAATATCTTGTCACACGTAAAATAAAAAATAAAAAATAA
ATAAAAATAAAATAAATAAAAATAATTATT
sequence update 2001. 3.22
Translated Amino Acid sequence 
vnvhyfedgnvqlntvtqkqttspsadaqstavnafkaigkaelnlhtaldnnystmgdt
tfkalrralpinrtkinwqkvknfkianelnk*i*n*ftscspily*ylvtrkiknkk*i
kik*IKIIIYLFTIKKK---

---VNVHYFEDGNVQLNTVTQKQTTSPSADAQSTAVNAFKAIGKAELNLHTALDNNYSTM
GDTTFKALRRALPINRTKINWQKVKNFKIANELNK*i*n*ftlvhqfyinilshvk*kik
nk*k*nk*k*l
Translated Amino Acid sequence (All Frames) 
Frame A:
s*csll*rw*csikhchsktnyftic*ctincsqci*snw*s*t*pphrt**qllhyg*y
hiqsp*tcssnqsyqnqlaksqklqncq*tq*inlklihflftnsilischt*nkk*kin
knkinknnylfiyn*kk---

---s*csll*rw*csikhchsktnyftic*ctincsqci*snw*s*t*pphrt**qllhy
g*yhiqsp*tcssnqsyqnqlaksqklqncq*tq*inlklihscspily*ylvtrkiknk
k*ikik*ikiii

Frame B:
vnvhyfedgnvqlntvtqkqttspsadaqstavnafkaigkaelnlhtaldnnystmgdt
tfkalrralpinrtkinwqkvknfkianelnk*i*n*ftscspily*ylvtrkiknkk*i
kik*IKIIIYLFTIKKK---

---VNVHYFEDGNVQLNTVTQKQTTSPSADAQSTAVNAFKAIGKAELNLHTALDNNYSTM
GDTTFKALRRALPINRTKINWQKVKNFKIANELNK*i*n*ftlvhqfyinilshvk*kik
nk*k*nk*k*l

Frame C:
lmfitlkmvmfn*tlslknkllhhllmhnqlqsmhlkqlvklnltstphlititplwvip
hskpldvlfqsivpkstgkksktsklpmnsinkfktnsllvhqfyinilshvk*kiknk*
k*nk*k*lfiylqlkkk---

---lmfitlkmvmfn*tlslknkllhhllmhnqlqsmhlkqlvklnltstphlititplw
viphskpldvlfqsivpkstgkksktsklpmnsinkfktnsllftnsilischt*nkk*k
inknkinknny
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSD303 (VSD303Q) /CSM/VS/VSD3-A/VSD303Q.Seq.d/                        666   0.0
VSI534 (VSI534Q) /CSM/VS/VSI5-B/VSI534Q.Seq.d/                        664   0.0
VFM721 (VFM721Q) /CSM/VF/VFM7-A/VFM721Q.Seq.d/                        664   0.0
VFM643 (VFM643Q) /CSM/VF/VFM6-B/VFM643Q.Seq.d/                        664   0.0
VFL683 (VFL683Q) /CSM/VF/VFL6-D/VFL683Q.Seq.d/                        664   0.0
SSB263 (SSB263Q) /CSM/SS/SSB2-C/SSB263Q.Seq.d/                        664   0.0
AHO350 (AHO350Q) /CSM/AH/AHO3-C/AHO350Q.Seq.d/                        664   0.0
SSI373 (SSI373Q) /CSM/SS/SSI3-D/SSI373Q.Seq.d/                        656   0.0
VFC326 (VFC326Q) /CSM/VF/VFC3-B/VFC326Q.Seq.d/                        587   e-166
CFE174 (CFE174Q) /CSM/CF/CFE1-D/CFE174Q.Seq.d/                        307   1e-82
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

M25132|M25132.1 D.discoideum CAP34 protein, complete cds.        664  0.0  2
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   507  e-175  6
AC143694|AC143694.1 Macaca mulatta clone CH250-269A10, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    42  0.004  2
BZ716005|BZ716005.1 OGEAN13TC ZM_0.7_1.5_KB Zea mays genomic clone ZMMBMa0218C02, DNA sequence.    36  0.014  2
AC027231|AC027231.1 Homo sapiens chromosome 14 clone RP11-276D21 map 14, LOW-PASS SEQUENCE SAMPLING.    40  0.021  2
AG226330|AG226330.1 Lotus corniculatus var. japonicus DNA, clone:LjB13n22_f.    36  0.036  2
AF328923|AF328923.1 Helicobacter pylori strain CR35 putative JHP1044-like protein gene, complete cds.    40  0.043  2
CC634164|CC634164.1 OGJBL08TV ZM_0.7_1.5_KB Zea mays genomic clone ZMMBMa0301A16, genomic survey sequence.    36  0.054  2
AZ863558|AZ863558.1 2M0171B10R Mouse 10kb plasmid UUGC1M library Mus musculus genomic clone UUGC2M0171B10 R, DNA sequence.    36  0.15  2
AX345050|AX345050.1 Sequence 121 from Patent WO0200928.           34  0.18  5
dna update 2003. 8.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P13022) RecName: Full=F-actin-capping protein subunit alpha; Al...   186   8e-46
BT072398_1(BT072398|pid:none) Salmo salar clone ssal-rgf-524-009...    91   6e-17
(Q2IBB9) RecName: Full=F-actin-capping protein subunit alpha-2; ...    90   8e-17
BT074988_1(BT074988|pid:none) Osmerus mordax clone omor-eva-508-...    89   1e-16
BT043615_1(BT043615|pid:none) Salmo salar clone HM4_3588 capping...    89   1e-16
BT075792_1(BT075792|pid:none) Caligus rogercresseyi clone crog-e...    89   2e-16
(Q4R959) RecName: Full=F-actin-capping protein subunit alpha-1; ...    89   2e-16
AK223226_1(AK223226|pid:none) Homo sapiens mRNA for F-actin capp...    89   2e-16
AY890784_1(AY890784|pid:none) Synthetic construct Homo sapiens c...    89   2e-16
BT078530_1(BT078530|pid:none) Lepeophtheirus salmonis Pacific fo...    89   2e-16
protein update 2009. 7.29
PSORT 

 psg: 0.58  gvh: 0.41  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: mitochondrial
	 12.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: vesicles of secretory system

>> prediction for VSD303 is cyt
5' end seq. ID VSD303F
5' end seq. 
>VSD303F.Seq
AGTTAATGTTCATTACTTTGAAGATGGTAATGTTCAATTAAACACTGTCACTCAAAAACA
AACTACTTCACCATCTGCTGATGCACAATCAACTGCAGTCAATGCATTTAAAGCAATTGG
TAAAGCTGAACTTAACCTCCACACCGCACTTGATAACAATTACTCCACTATGGGTGATAC
CACATTCAAAGCCCTTAGACGTGCTCTTCCAATCAATCGTACCAAAATCAACTGGCAAAA
AGTCAAAAACTTCAAAATTGCCAATGAACTCAATAAATAAATTTAAAACTAATTCACTTC
TTGTTCACCAATTCTATATTAATATCTTGTCACACGTAAAATAAAAAATAAAAAATAAAT
AAAAATAAAATAAATAAAAATAATTATTTATTTATTTACAATTAAAAAAAAAA-------
---
Length of 5' end seq. 413
3' end seq. ID VSD303Z
3' end seq. 
>VSD303Z.Seq
----------AGTTAATGTTCATTACTTTGAAGATGGTAATGTTCAATTAAACACTGTCA
CTCAAAAACAAACTACTTCACCATCTGCTGATGCACAATCAACTGCAGTCAATGCATTTA
AAGCAATTGGTAAAGCTGAACTTAACCTCCACACCGCACTTGATAACAATTACTCCACTA
TGGGTGATACCACATTCAAAGCCCTTAGACGTGCTCTTCCAATCAATCGTACCAAAATCA
ACTGGCAAAAAGTCAAAAACTTCAAAATTGCCAATGAACTCAATAAATAAATTTAAAACT
AATTCACTCTTGTTCACCAATTCTATATTAATATCTTGTCACACGTAAAATAAAAAATAA
AAAATAAATAAAAATAAAATAAATAAAAATAATTATT
Length of 3' end seq. 387
Connected seq. ID VSD303P
Connected seq. 
>VSD303P.Seq
AGTTAATGTTCATTACTTTGAAGATGGTAATGTTCAATTAAACACTGTCACTCAAAAACA
AACTACTTCACCATCTGCTGATGCACAATCAACTGCAGTCAATGCATTTAAAGCAATTGG
TAAAGCTGAACTTAACCTCCACACCGCACTTGATAACAATTACTCCACTATGGGTGATAC
CACATTCAAAGCCCTTAGACGTGCTCTTCCAATCAATCGTACCAAAATCAACTGGCAAAA
AGTCAAAAACTTCAAAATTGCCAATGAACTCAATAAATAAATTTAAAACTAATTCACTTC
TTGTTCACCAATTCTATATTAATATCTTGTCACACGTAAAATAAAAAATAAAAAATAAAT
AAAAATAAAATAAATAAAAATAATTATTTATTTATTTACAATTAAAAAAAAAA-------
---AGTTAATGTTCATTACTTTGAAGATGGTAATGTTCAATTAAACACTGTCACTCAAAA
ACAAACTACTTCACCATCTGCTGATGCACAATCAACTGCAGTCAATGCATTTAAAGCAAT
TGGTAAAGCTGAACTTAACCTCCACACCGCACTTGATAACAATTACTCCACTATGGGTGA
TACCACATTCAAAGCCCTTAGACGTGCTCTTCCAATCAATCGTACCAAAATCAACTGGCA
AAAAGTCAAAAACTTCAAAATTGCCAATGAACTCAATAAATAAATTTAAAACTAATTCAC
TCTTGTTCACCAATTCTATATTAATATCTTGTCACACGTAAAATAAAAAATAAAAAATAA
ATAAAAATAAAATAAATAAAAATAATTATT
Length of connected seq. 800
Full length Seq ID -
Full length Seq. -
Length of full length seq. -