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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSD274
Library VS
(Link to library)
Clone ID VSD274
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15476-1
Original site URL
Representative seq. ID VSD274P
(Link to Original site)
Representative DNA sequence 
>VSD274 (VSD274Q) /CSM/VS/VSD2-D/VSD274Q.Seq.d/
GATAATCAAAATGCCTCCAAAAGTAGATCCAAGCGAAAAAGTTGAAGTCTTCCTCAGAGT
ATGTGGTGGTGAAGCTGGTGCTATGTCCACCCTTGCACCAAAACTCGGTCCACTCGGTGT
TTCACCAAAGAAAGTTGGTGATGATATCGCTAAAGCCACTCAACCATGGAAAGGTATGAA
GGTTTCAGTCAAATTGACCATTCAAAATCGTATTGCTGTCCCAGAAGTTTTACCATCTGC
TTCAGCATTGGTCATCAAAGCCTTAAAAGAGCCACCAAGAGACAGAAAGAAGGAAAAGAA
CATTAAACACAACGGTAACATCCCATTAGAAGAAATCTGCAAAATCGCCAAGACCATGAG
ATTCAAATCTTTAGCTGTTGACTTCAAAGGTTCAGTCTTAGAAATCTTAGGTACTGCTCA
CTCTGTTGGTTGTAAAGTCAATGGTAAATCACCAAGAGATATCCAAGCTGGTATCCAATC
TGGTGAAATCGAAGTTGTCGAACCAAAATAAACTATTTAAACTCAAAATTTTAAATATAA
ATATAAAAAAAAAAXXXXXXXXXXCCACGCGTCCCGGATAATCAAAATGCCTCCAAAAGT
AGATCCAAGCGAAAAAGTTGAAGTCTTCCTCAGAGTATGTGGTGGTGAAGCTGGTGCTAT
GTCCACCCTTGCACCAAAACTCGGTCCACTCGGTGTTTCACCAAAGAAAGTTGGTGATGA
TATCGCTAAAGCCACTCAACCATGGAAAGGTATGAAGGTTTCAGTCAAATTGACCATTCA
AAATCGTATTGCTGTCCCAGAAGTTTTACCATCTGCTTCAGCATTGGTCATCAAAGCCTT
AAAAGAGCCACCAAGAGACAGAAAGAAGGAAAAGAACATTAAACACAACGGTAACATCCC
ATTAGAAGAAATCTGCAAAATCGCCAAGACCATGAGATTCAAATCTTTAGCTGTTGACTT
CAAAGGTTCAGTCTTAGAAATCTTAGGTACTGCTCACTCTGTTGGTTGTAAAGTCAATGG
TAAATCACCAAGAGATATCCAAGCTGGTATCCAATCTGGTGAAATCGAAGTTGTCGAACC
sequence update 2001. 3.22
Translated Amino Acid sequence 
*skclqk*iqakklkssseyvvvklvlcpplhqnsvhsvfhqrklvmislkplnhgkv*r
fqsn*pfkivllsqkfyhllqhwsskp*kshqeterrkrtlnttvtsh*kksaksprp*d
snl*lltskvqs*ks*VLLTLLVVKSMVNHQEISKLVSNLVKSKLSNQNKLFKLKILNIN
IKKK---

---HASRIIKMPPKVDPSEKVEVFLRVCGGEAGAMSTLAPKLGPLGVSPKKVGDDIAKAT
QPWKGMKVSVKLTIQNRIAVPEVLPSASALVIKALKEPPRDRKKEKNIKHNGNIPLEEIC
KIAKTMRFKSLAVDFKGSVLEILGTAHSVGCKVNGKSPRDIQAGIQSGEIEVVE
Translated Amino Acid sequence (All Frames) 
Frame A:
dnqnasksrskrks*slpqsmww*swcyvhpctktrstrcftkesw**yr*shstmerye
gfsqidhsksyccprsfticfsighqslkratkrqkegkeh*tqr*hpirrnlqnrqdhe
iqifsc*lqrfslrnlrycslcwl*sqw*itkrypswypiw*nrscrtkinylnskf*i*
i*kk---

---prvpdnqnasksrskrks*slpqsmww*swcyvhpctktrstrcftkesw**yr*sh
stmeryegfsqidhsksyccprsfticfsighqslkratkrqkegkeh*tqr*hpirrnl
qnrqdheiqifsc*lqrfslrnlrycslcwl*sqw*itkrypswypiw*nrscrt

Frame B:
iikmppkvdpsekvevflrvcggeagamstlapklgplgvspkkvgddiakatqpwkgmk
vsvkltiqnriavpevlpsasalvikalkepprdrkkeknikhngnipleeickiaktmr
fkslavdfkgsvleilgtahsvgckvngksprdiqagiqsgeievvepk*ti*tqnfkyk
ykkk---

---HASRIIKMPPKVDPSEKVEVFLRVCGGEAGAMSTLAPKLGPLGVSPKKVGDDIAKAT
QPWKGMKVSVKLTIQNRIAVPEVLPSASALVIKALKEPPRDRKKEKNIKHNGNIPLEEIC
KIAKTMRFKSLAVDFKGSVLEILGTAHSVGCKVNGKSPRDIQAGIQSGEIEVVE

Frame C:
*skclqk*iqakklkssseyvvvklvlcpplhqnsvhsvfhqrklvmislkplnhgkv*r
fqsn*pfkivllsqkfyhllqhwsskp*kshqeterrkrtlnttvtsh*kksaksprp*d
snl*lltskvqs*ks*VLLTLLVVKSMVNHQEISKLVSNLVKSKLSNQNKLFKLKILNIN
IKKK---

---trpg*skclqk*iqakklkssseyvvvklvlcpplhqnsvhsvfhqrklvmislkpl
nhgkv*rfqsn*pfkivllsqkfyhllqhwsskp*kshqeterrkrtlnttvtsh*kksa
ksprp*dsnl*lltskvqs*ks*vlltllvvksmvnhqeisklvsnlvksklsn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSD274 (VSD274Q) /CSM/VS/VSD2-D/VSD274Q.Seq.d/                       1078   0.0
VSF609 (VSF609Q) /CSM/VS/VSF6-A/VSF609Q.Seq.d/                       1076   0.0
VSC418 (VSC418Q) /CSM/VS/VSC4-A/VSC418Q.Seq.d/                       1072   0.0
VSA827 (VSA827Q) /CSM/VS/VSA8-B/VSA827Q.Seq.d/                       1072   0.0
VSA624 (VSA624Q) /CSM/VS/VSA6-A/VSA624Q.Seq.d/                       1072   0.0
VSA263 (VSA263Q) /CSM/VS/VSA2-C/VSA263Q.Seq.d/                       1065   0.0
VSB570 (VSB570Q) /CSM/VS/VSB5-C/VSB570Q.Seq.d/                       1061   0.0
VSA661 (VSA661Q) /CSM/VS/VSA6-C/VSA661Q.Seq.d/                       1061   0.0
VHA834 (VHA834Q) /CSM/VH/VHA8-B/VHA834Q.Seq.d/                       1061   0.0
VSH491 (VSH491Q) /CSM/VS/VSH4-D/VSH491Q.Seq.d/                       1059   0.0
own update 2003. 1. 9
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AX537004|AX537004.1 Sequence 605 from Patent WO02064766.          66  4e-28  5
AL398924|AL398924.1 T7 end of clone AS0AA012A08 of library AS0AA from strain CLIB 533 of Saccharomyces bayanus.    78  5e-18  4
AL402826|AL402826.1 T7 end of clone AT0AA004H11 of library AT0AA from strain CBS 4311 of Saccharomyces servazzii.    74  9e-17  2
AL392519|AL392519.1 T3 end of clone AR0AA005C02 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    76  3e-16  4
AL407551|AL407551.1 T7 end of clone AV0AA003A02 of library AV0AA from strain CBS 379 of Saccharomyces exiguus.    54  2e-14  4
AZ974567|AZ974567.1 2M0249O11F Mouse 10kb plasmid UUGC2M library Mus musculus genomic clone UUGC2M0249O11 F, DNA sequence.    80  2e-13  2
CB038812|CB038812.1 Tc_ad2_50C12_TEXF1 Teladorsagia circumcincta adults library 2 Teladorsagia circumcincta cDNA clone Tc_ad2_50C12 5' similar to O62290 Probable 60S ribosomal protein L12. Caenorhabditis elegans, mRNA sequence.    86  1e-12  1
CB039089|CB039089.1 Tc_ad2_53E11_TEXF1 Teladorsagia circumcincta adults library 2 Teladorsagia circumcincta cDNA clone Tc_ad2_53E11 5' similar to O62290 Probable 60S ribosomal protein L12. Caenorhabditis elegans, mRNA sequence.    86  1e-12  1
CB038824|CB038824.1 Tc_ad2_50E03_TEXF1 Teladorsagia circumcincta adults library 2 Teladorsagia circumcincta cDNA clone Tc_ad2_50E03 5' similar to O62290 Probable 60S ribosomal protein L12. Caenorhabditis elegans, mRNA sequence.    86  1e-12  1
CB038341|CB038341.1 Tc_ad2_44F06_TEXF1 Teladorsagia circumcincta adults library 2 Teladorsagia circumcincta cDNA clone Tc_ad2_44F06 5' similar to O62290 Probable 60S ribosomal protein L12. Caenorhabditis elegans, mRNA sequence.    86  1e-12  1
dna update 2003. 8.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK340119_1(AK340119|pid:none) Acyrthosiphon pisum ACYPI000059 mR...   222   2e-56
AE017343_705(AE017343|pid:none) Cryptococcus neoformans var. neo...   221   5e-56
AP007151_660(AP007151|pid:none) Aspergillus oryzae RIB40 genomic...   220   6e-56
AM269963_31(AM269963|pid:none) Aspergillus niger contig An01c016...   220   6e-56
CR761927_1(CR761927|pid:none) Xenopus tropicalis finished cDNA, ...   219   2e-55
BC041240_1(BC041240|pid:none) Xenopus laevis ribosomal protein L...   218   2e-55
AY961578_1(AY961578|pid:none) Lysiphlebus testaceipes ribosomal ...   217   5e-55
AM920428_319(AM920428|pid:none) Penicillium chrysogenum Wisconsi...   217   7e-55
AY496128_1(AY496128|pid:none) Capsicum annuum 60S ribosomal prot...   217   7e-55
EU963055_1(EU963055|pid:none) Zea mays clone 256949 60S ribosoma...   216   1e-54
protein update 2009. 7.29
PSORT 

 psg: 0.65  gvh: 0.52  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.09
 nuc: 0.12  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 28.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for VSD274 is nuc
5' end seq. ID VSD274F
5' end seq. 
>VSD274F.Seq
GATAATCAAAATGCCTCCAAAAGTAGATCCAAGCGAAAAAGTTGAAGTCTTCCTCAGAGT
ATGTGGTGGTGAAGCTGGTGCTATGTCCACCCTTGCACCAAAACTCGGTCCACTCGGTGT
TTCACCAAAGAAAGTTGGTGATGATATCGCTAAAGCCACTCAACCATGGAAAGGTATGAA
GGTTTCAGTCAAATTGACCATTCAAAATCGTATTGCTGTCCCAGAAGTTTTACCATCTGC
TTCAGCATTGGTCATCAAAGCCTTAAAAGAGCCACCAAGAGACAGAAAGAAGGAAAAGAA
CATTAAACACAACGGTAACATCCCATTAGAAGAAATCTGCAAAATCGCCAAGACCATGAG
ATTCAAATCTTTAGCTGTTGACTTCAAAGGTTCAGTCTTAGAAATCTTAGGTACTGCTCA
CTCTGTTGGTTGTAAAGTCAATGGTAAATCACCAAGAGATATCCAAGCTGGTATCCAATC
TGGTGAAATCGAAGTTGTCGAACCAAAATAAACTATTTAAACTCAAAATTTTAAATATAA
ATATAAAAAAAAAA----------
Length of 5' end seq. 554
3' end seq. ID VSD274Z
3' end seq. 
>VSD274Z.Seq
----------CCACGCGTCCCGGATAATCAAAATGCCTCCAAAAGTAGATCCAAGCGAAA
AAGTTGAAGTCTTCCTCAGAGTATGTGGTGGTGAAGCTGGTGCTATGTCCACCCTTGCAC
CAAAACTCGGTCCACTCGGTGTTTCACCAAAGAAAGTTGGTGATGATATCGCTAAAGCCA
CTCAACCATGGAAAGGTATGAAGGTTTCAGTCAAATTGACCATTCAAAATCGTATTGCTG
TCCCAGAAGTTTTACCATCTGCTTCAGCATTGGTCATCAAAGCCTTAAAAGAGCCACCAA
GAGACAGAAAGAAGGAAAAGAACATTAAACACAACGGTAACATCCCATTAGAAGAAATCT
GCAAAATCGCCAAGACCATGAGATTCAAATCTTTAGCTGTTGACTTCAAAGGTTCAGTCT
TAGAAATCTTAGGTACTGCTCACTCTGTTGGTTGTAAAGTCAATGGTAAATCACCAAGAG
ATATCCAAGCTGGTATCCAATCTGGTGAAATCGAAGTTGTCGAACC
Length of 3' end seq. 516
Connected seq. ID VSD274P
Connected seq. 
>VSD274P.Seq
GATAATCAAAATGCCTCCAAAAGTAGATCCAAGCGAAAAAGTTGAAGTCTTCCTCAGAGT
ATGTGGTGGTGAAGCTGGTGCTATGTCCACCCTTGCACCAAAACTCGGTCCACTCGGTGT
TTCACCAAAGAAAGTTGGTGATGATATCGCTAAAGCCACTCAACCATGGAAAGGTATGAA
GGTTTCAGTCAAATTGACCATTCAAAATCGTATTGCTGTCCCAGAAGTTTTACCATCTGC
TTCAGCATTGGTCATCAAAGCCTTAAAAGAGCCACCAAGAGACAGAAAGAAGGAAAAGAA
CATTAAACACAACGGTAACATCCCATTAGAAGAAATCTGCAAAATCGCCAAGACCATGAG
ATTCAAATCTTTAGCTGTTGACTTCAAAGGTTCAGTCTTAGAAATCTTAGGTACTGCTCA
CTCTGTTGGTTGTAAAGTCAATGGTAAATCACCAAGAGATATCCAAGCTGGTATCCAATC
TGGTGAAATCGAAGTTGTCGAACCAAAATAAACTATTTAAACTCAAAATTTTAAATATAA
ATATAAAAAAAAAA----------CCACGCGTCCCGGATAATCAAAATGCCTCCAAAAGT
AGATCCAAGCGAAAAAGTTGAAGTCTTCCTCAGAGTATGTGGTGGTGAAGCTGGTGCTAT
GTCCACCCTTGCACCAAAACTCGGTCCACTCGGTGTTTCACCAAAGAAAGTTGGTGATGA
TATCGCTAAAGCCACTCAACCATGGAAAGGTATGAAGGTTTCAGTCAAATTGACCATTCA
AAATCGTATTGCTGTCCCAGAAGTTTTACCATCTGCTTCAGCATTGGTCATCAAAGCCTT
AAAAGAGCCACCAAGAGACAGAAAGAAGGAAAAGAACATTAAACACAACGGTAACATCCC
ATTAGAAGAAATCTGCAAAATCGCCAAGACCATGAGATTCAAATCTTTAGCTGTTGACTT
CAAAGGTTCAGTCTTAGAAATCTTAGGTACTGCTCACTCTGTTGGTTGTAAAGTCAATGG
TAAATCACCAAGAGATATCCAAGCTGGTATCCAATCTGGTGAAATCGAAGTTGTCGAACC
Length of connected seq. 1070
Full length Seq ID -
Full length Seq. -
Length of full length seq. -