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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSD221
Library VS
(Link to library)
Clone ID VSD221
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VSD221P
(Link to Original site)
Representative DNA sequence 
>VSD221 (VSD221Q) /CSM/VS/VSD2-A/VSD221Q.Seq.d/
GATTTACTCAAAGGTGTCGTTTTAGGTTACAGAAGATCACAAGCTACTCAATACCCAAAC
ATCAGTTTAATCAAAATTGAAGGTGTTGTCTCACGTGAAGATTCAACTTTTTATTTAGGT
AAAAAAGTCTGTTTAGTCAGCAAAGTTTCCAAATCAGCCAAAAACCCAACTGGTCACAAA
ATTTCATGGGGTAAGATTTGCAAAACCCACGGTAGTTCAGGTGTTGTCCAAGCTCGTTTC
GCCAGAAACCTCCCACCAAAAGCTATGGGTGCCCCAGTCAGAGTTATGTTATACCCATCA
AACATCTAAATAAATCTTTAAAACGAAAAAAAAAATTATTTGAAAAAAAAAAXXXXXXXX
XXGATTTACTCAAAAGGTGTCGTTTTAGGTTACAGAAGATCACAAGCTACTCAATACCCA
AACATCAGTTTAATCAAAATTGAAGGTGTTGTCTCACGTGAAGATTCAACTTTTTATTTA
GGTAAAAAAGTCTGTTTAGTCAGCAAAGTTTCCAAATCAGCCAAAAACCCAACTGGTCAC
AAAATTTCATGGGGTAAGATTTGCAAAACCCACGGTAGTTCAGGTGTTGTCCAAGCTCGT
TTCGCCAGAAACCTCCCACCAAAAGCTATGGGTGCCCCAGTCAGAGTTATGTTATACCCA
TCAAACATCTAAATAATCTTNAAACGAAA
sequence update 2001. 3.22
Translated Amino Acid sequence 
ftqrcrfrlqkitsysipkhqfnqn*rcclt*rfnflfr*kslfsqqsfqisqkpnwsqn
fmg*dlqnpr*FRCCPSSFRQKPPTKSYGCPSQSYVIPIKHLNKSLKRKKKLFEKK---

---IYSKGVVLGYRRSQATQYPNISLIKIEGVVSREDSTFYLGKKVCLVSKVSKSAKNPT
GHKISWGKICKTHGSSGVVQARFARNLPPKAMGAPVRVMLYPSNI*iixkr
Translated Amino Acid sequence (All Frames) 
Frame A:
dllkgvvlgyrrsqatqypnislikiegvvsredstfylgkkvclvskvsksaknptghk
iswgkickthgssgvvqarfarnlppkamgapvrvmlypsni*inl*nekknylkkk---

---dllkrcrfrlqkitsysipkhqfnqn*rcclt*rfnflfr*kslfsqqsfqisqkpn
wsqnfmg*dlqnpr*frccpssfrqkpptksygcpsqsyvipikhlnnlxtk

Frame B:
iyskvsf*vtedhkllntqtsv*sklkvlshvkiqlfi*vkksv*sakfpnqpktqlvtk
fhgvrfakptvvqvlsklvspetshqklwvpqselcythqtsk*ifktkkkii*kkk---

---IYSKGVVLGYRRSQATQYPNISLIKIEGVVSREDSTFYLGKKVCLVSKVSKSAKNPT
GHKISWGKICKTHGSSGVVQARFARNLPPKAMGAPVRVMLYPSNI*iixkr

Frame C:
ftqrcrfrlqkitsysipkhqfnqn*rcclt*rfnflfr*kslfsqqsfqisqkpnwsqn
fmg*dlqnpr*FRCCPSSFRQKPPTKSYGCPSQSYVIPIKHLNKSLKRKKKLFEKK---

---ftqkvsf*vtedhkllntqtsv*sklkvlshvkiqlfi*vkksv*sakfpnqpktql
vtkfhgvrfakptvvqvlsklvspetshqklwvpqselcythqtsk*sxne
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ462 (VSJ462Q) /CSM/VS/VSJ4-C/VSJ462Q.Seq.d/                        642   0.0
VSD221 (VSD221Q) /CSM/VS/VSD2-A/VSD221Q.Seq.d/                        642   0.0
VSI656 (VSI656Q) /CSM/VS/VSI6-C/VSI656Q.Seq.d/                        630   e-180
VSG169 (VSG169Q) /CSM/VS/VSG1-C/VSG169Q.Seq.d/                        624   e-178
VSK108 (VSK108Q) /CSM/VS/VSK1-A/VSK108Q.Seq.d/                        622   e-177
VSJ792 (VSJ792Q) /CSM/VS/VSJ7-D/VSJ792Q.Seq.d/                        622   e-177
VSJ573 (VSJ573Q) /CSM/VS/VSJ5-D/VSJ573Q.Seq.d/                        622   e-177
VSJ438 (VSJ438Q) /CSM/VS/VSJ4-B/VSJ438Q.Seq.d/                        622   e-177
VSJ322 (VSJ322Q) /CSM/VS/VSJ3-A/VSJ322Q.Seq.d/                        622   e-177
VSJ132 (VSJ132Q) /CSM/VS/VSJ1-B/VSJ132Q.Seq.d/                        622   e-177
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BQ834795|BQ834795.1 Po_ad_03E06_TEXF1 Psoroptes ovis mixed Psoroptes ovis cDNA clone Po_ad_03E06 5' similar to BAB27124 ES CELLS CDNA, RIKEN FULL-LENGTH ENRICHE FULL INSERT, mRNA sequence.    36  0.13  2
D17450|D17450.1 Mycoplasma salivarium gene for aminopeptidase, complete cds.    34  1.4  2
AX344895|AX344895.1 Sequence 320 from Patent WO0200927.           40  4.0  2
AX323826|AX323826.1 Sequence 314 from Patent WO0192565.           40  4.0  2
BM023621|BM023621.1 ie82g07.y1 Melton Normalized Human Islet 4 N4-HIS 1 Homo sapiens cDNA clone IMAGE:5673421 5', mRNA sequence.    32  4.4  3
BF574666|BF574666.1 602132142F1 NIH_MGC_81 Homo sapiens cDNA clone IMAGE:4271554 5', mRNA sequence.    32  4.7  3
AP002899|AP002899.2 Oryza sativa genomic DNA, chromosome 1, PAC clone:P0456A01.    36  4.8  2
AP003142|AP003142.2 Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 1, PAC clone:P0435H01.    36  5.3  2
CD002652| ; putative ABC transporter; protein id: At4g04770.1, supported by cDNA: gi_15529142, supported by cDNA: gi_15912264 [Arabidopsis thaliana] [ E- value 8.00E-76 ], mRNA sequence.    36  5.3  2
BG485300|BG485300.1 602504360F1 NIH_MGC_77 Homo sapiens cDNA clone IMAGE:4617662 5', mRNA sequence.    32  6.8  3
dna update 2003. 8.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FB899478_1(FB899478|pid:none) Sequence 118751 from Patent WO2008...   102   8e-21
FB899466_1(FB899466|pid:none) Sequence 118739 from Patent WO2008...   102   1e-20
FJ362592_1(FJ362592|pid:none) Vernicia fordii 60S ribosomal prot...   102   1e-20
EF144854_1(EF144854|pid:none) Populus trichocarpa clone WS01119_...   102   1e-20
(Q9FZH0) RecName: Full=60S ribosomal protein L35a-2;  &AC008046_...   102   1e-20
FB899468_1(FB899468|pid:none) Sequence 118741 from Patent WO2008...   102   1e-20
(P51422) RecName: Full=60S ribosomal protein L35a-4;  &AF446895_...   101   2e-20
AY857450_1(AY857450|pid:none) Suberites domuncula L35a mRNA, com...   101   2e-20
(Q9C912) RecName: Full=60S ribosomal protein L35a-3;  &AC020579_...   101   2e-20
BT051287_1(BT051287|pid:none) Medicago truncatula clone MTYF1_F2...   100   4e-20
protein update 2009. 7.29
PSORT 

 psg: 0.70  gvh: 0.33  alm: 0.53  top: 0.53  tms: 0.00  mit: 0.48  mip: 0.10
 nuc: 0.20  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: mitochondrial
	 24.0 %: nuclear
	 16.0 %: cytoplasmic
	  4.0 %: endoplasmic reticulum

>> prediction for VSD221 is mit
5' end seq. ID VSD221F
5' end seq. 
>VSD221F.Seq
GATTTACTCAAAGGTGTCGTTTTAGGTTACAGAAGATCACAAGCTACTCAATACCCAAAC
ATCAGTTTAATCAAAATTGAAGGTGTTGTCTCACGTGAAGATTCAACTTTTTATTTAGGT
AAAAAAGTCTGTTTAGTCAGCAAAGTTTCCAAATCAGCCAAAAACCCAACTGGTCACAAA
ATTTCATGGGGTAAGATTTGCAAAACCCACGGTAGTTCAGGTGTTGTCCAAGCTCGTTTC
GCCAGAAACCTCCCACCAAAAGCTATGGGTGCCCCAGTCAGAGTTATGTTATACCCATCA
AACATCTAAATAAATCTTTAAAACGAAAAAAAAAATTATTTGAAAAAAAAAA--------
--
Length of 5' end seq. 352
3' end seq. ID VSD221Z
3' end seq. 
>VSD221Z.Seq
----------GATTTACTCAAAAGGTGTCGTTTTAGGTTACAGAAGATCACAAGCTACTC
AATACCCAAACATCAGTTTAATCAAAATTGAAGGTGTTGTCTCACGTGAAGATTCAACTT
TTTATTTAGGTAAAAAAGTCTGTTTAGTCAGCAAAGTTTCCAAATCAGCCAAAAACCCAA
CTGGTCACAAAATTTCATGGGGTAAGATTTGCAAAACCCACGGTAGTTCAGGTGTTGTCC
AAGCTCGTTTCGCCAGAAACCTCCCACCAAAAGCTATGGGTGCCCCAGTCAGAGTTATGT
TATACCCATCAAACATCTAAATAATCTTNAAACGAAA
Length of 3' end seq. 327
Connected seq. ID VSD221P
Connected seq. 
>VSD221P.Seq
GATTTACTCAAAGGTGTCGTTTTAGGTTACAGAAGATCACAAGCTACTCAATACCCAAAC
ATCAGTTTAATCAAAATTGAAGGTGTTGTCTCACGTGAAGATTCAACTTTTTATTTAGGT
AAAAAAGTCTGTTTAGTCAGCAAAGTTTCCAAATCAGCCAAAAACCCAACTGGTCACAAA
ATTTCATGGGGTAAGATTTGCAAAACCCACGGTAGTTCAGGTGTTGTCCAAGCTCGTTTC
GCCAGAAACCTCCCACCAAAAGCTATGGGTGCCCCAGTCAGAGTTATGTTATACCCATCA
AACATCTAAATAAATCTTTAAAACGAAAAAAAAAATTATTTGAAAAAAAAAA--------
--GATTTACTCAAAAGGTGTCGTTTTAGGTTACAGAAGATCACAAGCTACTCAATACCCA
AACATCAGTTTAATCAAAATTGAAGGTGTTGTCTCACGTGAAGATTCAACTTTTTATTTA
GGTAAAAAAGTCTGTTTAGTCAGCAAAGTTTCCAAATCAGCCAAAAACCCAACTGGTCAC
AAAATTTCATGGGGTAAGATTTGCAAAACCCACGGTAGTTCAGGTGTTGTCCAAGCTCGT
TTCGCCAGAAACCTCCCACCAAAAGCTATGGGTGCCCCAGTCAGAGTTATGTTATACCCA
TCAAACATCTAAATAATCTTNAAACGAAA
Length of connected seq. 679
Full length Seq ID -
Full length Seq. -
Length of full length seq. -