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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSD193
Library VS
(Link to library)
Clone ID VSD193
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U10129-1
Original site URL
Representative seq. ID VSD193E
(Link to Original site)
Representative DNA sequence 
>VSD193 (VSD193Q) /CSM/VS/VSD1-D/VSD193Q.Seq.d/
GTTTATTAAAAAATGAAATCAATTTCTTTTATTTGTTTATTAATTATTGGATTTTTATTA
ATTGGAAATAGTTTTGCTAACGATTCAATTAAATCAGATGAAATCACATGTAATTTGTGT
AATGCTATTGTAGGATTTGCAGAAAAATATGTTACTACAAATGCTACTGAACAAGAATTA
ATTAAAAAACTTGATGACTATTGCGGAATATTACCATCAGAATTTAGTCAATCTTGTGTT
GAAACAGTTAATAACTATGGTGTATTAATTATTAGATTATTAATTAATAAAGAAGATGCA
GATAATATTTGTATAATGATTGAATTATGTCCAAAATCATCATCATCATCACCATCATTA
TTTTTAATTAATGAGAATAATAGTAATAGTAATGGCACAGATTGTGAAATTTGTACATTC
ATTACTAAATATGCTGAAAATTTATTAGAAGCAAATAAAACAATTGAAGATATTGTAAAA
GTTGTTGATGATTTCTGTAAAGTTTTACCATCTCAATATAAGACTGATTGTGTTGCCATG
GCTTCAAATTATATTCCAGCAATTGTTAAAATGTTGGAGAACAATTATAATGCAGAACAA
GTTTGTCAAAAGTTAAGTTTTTGTTTACCACCACCACCACCATCAACTGAAGGTACCACT
CTATGTGATCTTTGTTTAGATTTCGCTAATGATGCTATTGAAATTGTTGAAAATTCAGGT
GATGTTGAAGCACAACTTTTAAAAGAATGTAAATACTTTTTCTCATTCATACCAACAATT
GAAAATCAATGTGAAAAAATTATAAGTGAAAATTTTGATGCAATTGTTAATGAAATTCAA
GATCACATAAATCCAACCTCTCTTTGCCAAAATTTCCTCCATATTTGTAATTAATCATTT
GATTAATAAATACAAATATATTTATATCACTAAAATTTAAAATAAAATAAAATAAAAATA
AAAGNAAAATAAAAACATT
sequence update 2001. 3.23
Translated Amino Acid sequence 
vy*KMKSISFICLLIIGFLLIGNSFANDSIKSDEITCNLCNAIVGFAEKYVTTNATEQEL
IKKLDDYCGILPSEFSQSCVETVNNYGVLIIRLLINKEDADNICIMIELCPKSSSSSPSL
FLINENNSNSNGTDCEICTFITKYAENLLEANKTIEDIVKVVDDFCKVLPSQYKTDCVAM
ASNYIPAIVKMLENNYNAEQVCQKLSFCLPPPPPSTEGTTLCDLCLDFANDAIEIVENSG
DVEAQLLKECKYFFSFIPTIENQCEKIISENFDAIVNEIQDHINPTSLCQNFLHICN*sf
d**iqiylyh*nlk*nkikikxk*kh
Translated Amino Acid sequence (All Frames) 
Frame A:
vy*KMKSISFICLLIIGFLLIGNSFANDSIKSDEITCNLCNAIVGFAEKYVTTNATEQEL
IKKLDDYCGILPSEFSQSCVETVNNYGVLIIRLLINKEDADNICIMIELCPKSSSSSPSL
FLINENNSNSNGTDCEICTFITKYAENLLEANKTIEDIVKVVDDFCKVLPSQYKTDCVAM
ASNYIPAIVKMLENNYNAEQVCQKLSFCLPPPPPSTEGTTLCDLCLDFANDAIEIVENSG
DVEAQLLKECKYFFSFIPTIENQCEKIISENFDAIVNEIQDHINPTSLCQNFLHICN*sf
d**iqiylyh*nlk*nkikikxk*kh


Frame B:
fikk*nqfllfvy*lldfy*leivlltiqlnqmkshvicvmll*dlqknmllqmllnkn*
lknlmtiaeyyhqnlvnlvlkqlitmvy*lldy*likkmqiifv**lnyvqnhhhhhhhy
f*lmriivivmaqivkfvhsllnmlkiy*kqikqlkil*kllmisvkfyhlnirlivlpw
lqiifqqllkcwrtiimqnkfvks*vfvyhhhhhqlkvplyvifv*islmmllkllkiqv
mlkhnf*knvntfshsyqqlkinvkkl*vkilmqllmkfkit*iqplfakissifvinhl
inkykyiyitki*nkik*k*kxnkni


Frame C:
llkneinffylfinywifinwk*fc*rfn*ir*nhm*fv*cycricrkicyykcy*trin
*kt**llrnitiri*silc*ns**lwciny*iin**rrcr*ylynd*imskiiiiitiii
fn**e*****whrl*nlyihy*ic*kfirsk*nn*rycksc**fl*sftisi*d*lcchg
fklyssnc*nvgeql*crtslskvkflftttttin*ryhsm*slfrfr**cy*nc*kfr*
c*sttfkrm*ilflihtnn*ksm*knyk*kf*cnc**nsrshksnlslpkfppyl*lii*
lintnifislkfkik*nknkxkikt
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSD193 (VSD193Q) /CSM/VS/VSD1-D/VSD193Q.Seq.d/                       1076   0.0
FC-IC1312 (FC-IC1312Q) /CSM/FC-IC/FC-IC1312Q.Seq.d/                   490   e-137
VSH542 (VSH542Q) /CSM/VS/VSH5-B/VSH542Q.Seq.d/                        385   e-106
VFO888 (VFO888Q) /CSM/VF/VFO8-D/VFO888Q.Seq.d/                        159   1e-37
VFB595 (VFB595Q) /CSM/VF/VFB5-D/VFB595Q.Seq.d/                        157   4e-37
VFB732 (VFB732Q) /CSM/VF/VFB7-B/VFB732Q.Seq.d/                         84   5e-15
VFB590 (VFB590Q) /CSM/VF/VFB5-D/VFB590Q.Seq.d/                         82   2e-14
VFJ635 (VFJ635Q) /CSM/VF/VFJ6-B/VFJ635Q.Seq.d/                         80   7e-14
VFH555 (VFH555Q) /CSM/VF/VFH5-C/VFH555Q.Seq.d/                         66   1e-09
VFG662 (VFG662Q) /CSM/VF/VFG6-C/VFG662Q.Seq.d/                         66   1e-09
own update 2001.12. 5
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U66367|U66367.1 Dictyostelium discoideum SapA (sapA) mRNA, partial cds.   107  3e-51  4
AC117076|AC117076.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.    36  6e-05  11
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    46  1e-04  10
AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.    38  3e-04  10
AL844507|AL844507.1 Plasmodium falciparum chromosome 8.           38  5e-04  13
AR300198|AR300198.1 Sequence 1 from patent US 6537773.            36  6e-04  14
AC117176|AC117176.2 Dictyostelium discoideum chromosome 2 map 5018074-5200947 strain AX4, complete sequence.    36  6e-04  10
AC117080|AC117080.2 Dictyostelium discoideum chromosome 2 map complement(821514-735598) strain AX4, complete sequence.    38  8e-04  6
AC117070|AC117070.2 Dictyostelium discoideum chromosome 2 map 2097701-2233941 strain AX4, complete sequence.    38  0.004  11
AC117082|AC117082.2 Dictyostelium discoideum chromosome 2 map complement(6807709-6776860) strain AX4, complete sequence.    36  0.004  6
dna update 2003. 9.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U66367_1(U66367|pid:none) Dictyostelium discoideum SapA (sapA) m...   183   7e-45
BC105409_1(BC105409|pid:none) Bos taurus prosaposin, mRNA (cDNA ...   102   2e-20
AF108656_1(AF108656|pid:none) Gallus gallus prosaposin mRNA, com...    99   3e-19
(O13035) RecName: Full=Proactivator polypeptide; Contains:   Rec...    99   3e-19
AB209776_1(AB209776|pid:none) Homo sapiens mRNA for prosaposin v...    97   8e-19
AK223290_1(AK223290|pid:none) Homo sapiens mRNA for prosaposin (...    97   8e-19
CR861454_1(CR861454|pid:none) Pongo abelii mRNA; cDNA DKFZp459G1...    97   8e-19
M60258_1(M60258|pid:none) Human mutant cerebroside sulfate activ...    97   8e-19
(Q6IBQ6) RecName: Full=Proactivator polypeptide; Contains:   Rec...    97   8e-19
M60257_1(M60257|pid:none) Human mutant cerebroside sulfate activ...    97   8e-19
protein update 2009. 7.29
PSORT 

 psg: 0.99  gvh: 0.72  alm: 0.39  top: 0.20  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: extracellular, including cell wall
	 16.0 %: cytoplasmic
	 12.0 %: vacuolar
	  8.0 %: endoplasmic reticulum
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system
	  4.0 %: nuclear

>> prediction for VSD193 is exc
5' end seq. ID VSD193F
5' end seq. 
>VSD193F.Seq
GTTTATTAAAAAATGAAATCAATTTCTTTTATTTGTTTATTAATTATTGGATTTTTATTA
ATTGGAAATAGTTTTGCTAACGATTCAATTAAATCAGATGAAATCACATGTAATTTGTGT
AATGCTATTGTAGGATTTGCAGAAAAATATGTTACTACAAATGCTACTGAACAAGAATTA
ATTAAAAAACTTGATGACTATTGCGGAATATTACCATCAGAATTTAGTCAATCTTGTGTT
GAAACAGTTAATAACTATGGTGTATTAATTATTAGATTATTAATTAATAAAGAAGATGCA
GATAATATTTGTATAATGATTGAATTATGTCCAAAATCATCATCATCATCACCATCATTA
TTTTTAATTAATGAGAATAATAGTAATAGTAATGGCACAGATTGTGAAATTTGTACATTC
ATTACTAAATATGCTGAAAATTTATTAGAAGCAAATAAAACAATTGAAGATATTGTAAAA
GTTGTTGATGATTTCTGTAAAGTTTTACCATCTCAATATAAGACTGATTGTG--------
--
Length of 5' end seq. 532
3' end seq. ID VSD193Z
3' end seq. 
>VSD193Z.Seq
----------CATCATCATCATCACCATCATTATTTTTAATTAATGAGAATAATAGTAAT
AGTAATGGCACAGATTGTGAAATTTGTACATTCATTACTAAATATGCTGAAAATTTATTA
GAAGCAAATAAAACAATTGAAGATATTGTAAAAGTTGTTGATGATTTCTGTAAAGTTTTA
CCATCTCAATATAAGACTGATTGTGTTGCCATGGCTTCAAATTATATTCCAGCAATTGTT
AAAATGTTGGAGAACAATTATAATGCAGAACAAGTTTGTCAAAAGTTAAGTTTTTGTTTA
CCACCACCACCACCATCAACTGAAGGTACCACTCTATGTGATCTTTGTTTAGATTTCGCT
AATGATGCTATTGAAATTGTTGAAAATTCAGGTGATGTTGAAGCACAACTTTTAAAAGAA
TGTAAATACTTTTTCTCATTCATACCAACAATTGAAAATCAATGTGAAAAAATTATAAGT
GAAAATTTTGATGCAATTGTTAATGAAATTCAAGATCACATAAATCCAACCTCTCTTTGC
CAAAATTTCCTCCATATTTGTAATTAATCATTTGATTAATAAATACAAATATATTTATAT
CACTAAAATTTAAAATAAAATAAAATAAAAATAAAAGNAAAATAAAAACATT
Length of 3' end seq. 642
Connected seq. ID VSD193P
Connected seq. 
>VSD193P.Seq
GTTTATTAAAAAATGAAATCAATTTCTTTTATTTGTTTATTAATTATTGGATTTTTATTA
ATTGGAAATAGTTTTGCTAACGATTCAATTAAATCAGATGAAATCACATGTAATTTGTGT
AATGCTATTGTAGGATTTGCAGAAAAATATGTTACTACAAATGCTACTGAACAAGAATTA
ATTAAAAAACTTGATGACTATTGCGGAATATTACCATCAGAATTTAGTCAATCTTGTGTT
GAAACAGTTAATAACTATGGTGTATTAATTATTAGATTATTAATTAATAAAGAAGATGCA
GATAATATTTGTATAATGATTGAATTATGTCCAAAATCATCATCATCATCACCATCATTA
TTTTTAATTAATGAGAATAATAGTAATAGTAATGGCACAGATTGTGAAATTTGTACATTC
ATTACTAAATATGCTGAAAATTTATTAGAAGCAAATAAAACAATTGAAGATATTGTAAAA
GTTGTTGATGATTTCTGTAAAGTTTTACCATCTCAATATAAGACTGATTGTG--------
--CATCATCATCATCACCATCATTATTTTTAATTAATGAGAATAATAGTAATAGTAATGG
CACAGATTGTGAAATTTGTACATTCATTACTAAATATGCTGAAAATTTATTAGAAGCAAA
TAAAACAATTGAAGATATTGTAAAAGTTGTTGATGATTTCTGTAAAGTTTTACCATCTCA
ATATAAGACTGATTGTGTTGCCATGGCTTCAAATTATATTCCAGCAATTGTTAAAATGTT
GGAGAACAATTATAATGCAGAACAAGTTTGTCAAAAGTTAAGTTTTTGTTTACCACCACC
ACCACCATCAACTGAAGGTACCACTCTATGTGATCTTTGTTTAGATTTCGCTAATGATGC
TATTGAAATTGTTGAAAATTCAGGTGATGTTGAAGCACAACTTTTAAAAGAATGTAAATA
CTTTTTCTCATTCATACCAACAATTGAAAATCAATGTGAAAAAATTATAAGTGAAAATTT
TGATGCAATTGTTAATGAAATTCAAGATCACATAAATCCAACCTCTCTTTGCCAAAATTT
CCTCCATATTTGTAATTAATCATTTGATTAATAAATACAAATATATTTATATCACTAAAA
TTTAAAATAAAATAAAATAAAAATAAAAGNAAAATAAAAACATT
Length of connected seq. 1174
Full length Seq ID VSD193E
Full length Seq. 
>VSD193E.Seq
GTTTATTAAAAAATGAAATCAATTTCTTTTATTTGTTTATTAATTATTGGATTTTTATTA
ATTGGAAATAGTTTTGCTAACGATTCAATTAAATCAGATGAAATCACATGTAATTTGTGT
AATGCTATTGTAGGATTTGCAGAAAAATATGTTACTACAAATGCTACTGAACAAGAATTA
ATTAAAAAACTTGATGACTATTGCGGAATATTACCATCAGAATTTAGTCAATCTTGTGTT
GAAACAGTTAATAACTATGGTGTATTAATTATTAGATTATTAATTAATAAAGAAGATGCA
GATAATATTTGTATAATGATTGAATTATGTCCAAAATCATCATCATCATCACCATCATTA
TTTTTAATTAATGAGAATAATAGTAATAGTAATGGCACAGATTGTGAAATTTGTACATTC
ATTACTAAATATGCTGAAAATTTATTAGAAGCAAATAAAACAATTGAAGATATTGTAAAA
GTTGTTGATGATTTCTGTAAAGTTTTACCATCTCAATATAAGACTGATTGTGTTGCCATG
GCTTCAAATTATATTCCAGCAATTGTTAAAATGTTGGAGAACAATTATAATGCAGAACAA
GTTTGTCAAAAGTTAAGTTTTTGTTTACCACCACCACCACCATCAACTGAAGGTACCACT
CTATGTGATCTTTGTTTAGATTTCGCTAATGATGCTATTGAAATTGTTGAAAATTCAGGT
GATGTTGAAGCACAACTTTTAAAAGAATGTAAATACTTTTTCTCATTCATACCAACAATT
GAAAATCAATGTGAAAAAATTATAAGTGAAAATTTTGATGCAATTGTTAATGAAATTCAA
GATCACATAAATCCAACCTCTCTTTGCCAAAATTTCCTCCATATTTGTAATTAATCATTT
GATTAATAAATACAAATATATTTATATCACTAAAATTTAAAATAAAATAAAATAAAAATA
AAAGNAAAATAAAAACATT
Length of full length seq. 979