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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSD185
Library VS
(Link to library)
Clone ID VSD185
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16229-1
Original site URL
Representative seq. ID VSD185E
(Link to Original site)
Representative DNA sequence 
>VSD185 (VSD185Q) /CSM/VS/VSD1-D/VSD185Q.Seq.d/
AAAATATAGAAAATGTCAGGTGCTTTAAAAAATTTAGTTAAATCAGTTATTAAAGTTGGT
GAAAAAGTTCCAATGGATGTTACTTTAGGTAAAGCACTCCCACCAGTCGATGGTGTTTGT
GCAATGGCACCAAAAGTTTTATCAGGTGAACTTTTCAAAGACCGTAAAGTTGTTTTATTC
GCTGTTCCAGGTGCTTTTACTCCAACTTGTTCAGCCAAACATTTACCAGGTTTTATTGAA
AAATCAGAAGAAATTAAAAAAAAAGGTATCTCTGAAATCTTTTGTATTGCCACCAATGAC
CCATTTGTTATGAGTGCTTGGGGTAAAGACGTTAATGCAGGTACTGCAGTTACTTTACTT
TCAGATGGTAACTCTGAATTCACTAAAAAGATTGGACTTGAAATGGATGGTAAGGCTTTC
TTACTTGGTGAAGACAGATCTCAAAGATATGCTATGATCCTTGACTCTGGTGTTGTCAAA
CATCTTGCAGTTGAAGAAGGTGGTAAATTTATGTATCATCTGCTAAGCAATCTTAAAACA
ATTATAAACATAAAATCAATCTA
sequence update 2001. 3.25
Translated Amino Acid sequence 
ki*KMSGALKNLVKSVIKVGEKVPMDVTLGKALPPVDGVCAMAPKVLSGELFKDRKVVLF
AVPGAFTPTCSAKHLPGFIEKSEEIKKKGISEIFCIATNDPFVMSAWGKDVNAGTAVTLL
SDGNSEFTKKIGLEMDGKAFLLGEDRSQRYAMILDSGVVKHLAVEEGGKFMYHLLSNLKT
IINIKSI
Translated Amino Acid sequence (All Frames) 
Frame A:
ki*KMSGALKNLVKSVIKVGEKVPMDVTLGKALPPVDGVCAMAPKVLSGELFKDRKVVLF
AVPGAFTPTCSAKHLPGFIEKSEEIKKKGISEIFCIATNDPFVMSAWGKDVNAGTAVTLL
SDGNSEFTKKIGLEMDGKAFLLGEDRSQRYAMILDSGVVKHLAVEEGGKFMYHLLSNLKT
IINIKSI


Frame B:
kyrkcqvl*ki*lnqllklvkkfqwmll*vkhshqsmvfvqwhqkfyqvnfsktvklfys
lfqvlllqlvqpniyqvllknqkklkkkvslksfvlppmthll*vlgvktlmqvlqllyf
qmvtlnslkrldlkwmvrlsylvktdlkdml*sltlvlsnilqlkkvvnlciic*ailkq
l*t*nqs


Frame C:
nienvrcfkkfs*isy*sw*kssngcyfr*stptsrwclcngtksfir*tfqrp*scfir
csrcfysnlfsqtftrfy*kirrn*kkryl*nllychq*picyeclg*rr*crycsyftf
rw*l*ih*kdwt*ngw*gfltw*rqiskicydp*lwccqtscs*rrw*iyvssakqs*nn
ykhkinl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSD185 (VSD185Q) /CSM/VS/VSD1-D/VSD185Q.Seq.d/                       1063   0.0
VSD213 (VSD213Q) /CSM/VS/VSD2-A/VSD213Q.Seq.d/                       1035   0.0
SSG643 (SSG643Q) /CSM/SS/SSG6-B/SSG643Q.Seq.d/                       1029   0.0
VHF501 (VHF501Q) /CSM/VH/VHF5-A/VHF501Q.Seq.d/                       1003   0.0
VSA450 (VSA450Q) /CSM/VS/VSA4-C/VSA450Q.Seq.d/                        965   0.0
SSF753 (SSF753Q) /CSM/SS/SSF7-C/SSF753Q.Seq.d/                        957   0.0
SHJ382 (SHJ382Q) /CSM/SH/SHJ3-D/SHJ382Q.Seq.d/                        950   0.0
VSH776 (VSH776Q) /CSM/VS/VSH7-D/VSH776Q.Seq.d/                        940   0.0
VHJ449 (VHJ449Q) /CSM/VH/VHJ4-C/VHJ449Q.Seq.d/                        926   0.0
SSE376 (SSE376Q) /CSM/SS/SSE3-D/SSE376Q.Seq.d/                        918   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BD283099|BD283099.1 Flea head, nerve cord, hindgut and malpighian tubule nucleic acid molecules, proteins and uses thereof.    48  2e-06  2
BF779954|BF779954.1 3254-25 hindgut and Malpighian tubule cDNA library Ctenocephalides felis cDNA clone 3254-25, mRNA sequence.    48  2e-06  2
BQ476622|BQ476622.1 curculio3e06.g Curculio glandium cDNA Curculio glandium cDNA clone curculio3e06 3', mRNA sequence.    46  0.46  1
BQ476340|BQ476340.1 curculio3e06.b Curculio glandium cDNA Curculio glandium cDNA clone curculio3e06 5', mRNA sequence.    46  0.46  1
AX345182|AX345182.1 Sequence 253 from Patent WO0200928.           46  0.46  1
AC109206|AC109206.5 Mus musculus clone RP23-142F22, WORKING DRAFT SEQUENCE, 3 ordered pieces.    42  1.2  2
BX004766|BX004766.9 Zebrafish DNA sequence from clone DKEY-5P1 in linkage group 20.    46  1.3  2
AC094645|AC094645.5 Rattus norvegicus clone CH230-4M23, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    38  1.5  4
AP004174|AP004174.1 Mycoplasma penetrans genomic DNA, complete sequence, section 5/5.    38  1.7  6
AL353730|AL353730.13 Human DNA sequence from clone RP11-55P9 on chromosome 9 Contains  GSSs and STSs.    44  1.8  1
dna update 2003. 9.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000655_172(CP000655|pid:none) Polynucleobacter necessarius sub...   128   9e-29
CP001010_161(CP001010|pid:none) Polynucleobacter necessarius sub...   127   2e-28
CP000248_1994(CP000248|pid:none) Novosphingobium aromaticivorans...   126   5e-28
AE015451_2674(AE015451|pid:none) Pseudomonas putida KT2440 compl...   125   6e-28
BX640426_146(BX640426|pid:none) Bordetella parapertussis strain ...   124   1e-27
CP000712_3003(CP000712|pid:none) Pseudomonas putida F1, complete...   124   2e-27
AE005673_3368(AE005673|pid:none) Caulobacter crescentus CB15, co...   122   5e-27
CP000613_3568(CP000613|pid:none) Rhodospirillum centenum SW, com...   122   7e-27
AE017282_41(AE017282|pid:none) Methylococcus capsulatus str. Bat...   122   9e-27
CP000158_1040(CP000158|pid:none) Hyphomonas neptunium ATCC 15444...   121   1e-26
protein update 2009. 7.29
PSORT 

 psg: 0.59  gvh: 0.46  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 28.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VSD185 is cyt
5' end seq. ID VSD185F
5' end seq. 
>VSD185F.Seq
AAAATATAGAAAATGTCAGGTGCTTTAAAAAATTTAGTTAAATCAGTTATTAAAGTTGGT
GAAAAAGTTCCAATGGATGTTACTTTAGGTAAAGCACTCCCACCAGTCGATGGTGTTTGT
GCAATGGCACCAAAAGTTTTATCAGGTGAACTTTTCAAAGACCGTAAAGTTGTTTTATTC
GCTGTTCCAGGTGCTTTTACTCCAACTTGTTCAGCCAAACATTTACCAGGTTTTATTGAA
AAATCAGAAGAAATTAAAAAAAAAGGTATCTCTGAAATCTTTTGTATTGCCACCAATGAC
CCATTTGTTATGAGTGCTTGGGGTAAAGACGTTAATGCAGGTACTGCAGTTACTTTACTT
TCAGATGGTAACTCTGAATTCACTAAAAAGATTGGACTTGAAATGGATGGTAAGGCTTTC
TTACTTGGTGAAGACAGATCTCAAAGATATGCTATGATCCTTGACTCTGGTGTTGTCAAA
CATCTTGCAGTTGAAGAA----------
Length of 5' end seq. 498
3' end seq. ID VSD185Z
3' end seq. 
>VSD185Z.Seq
----------AAAATATAGAAAATGTCAGGTGCTTTAAAAAATTTAGTTAAATCAGTTAT
TAAAGTTGGTGAAAAAGTTCCAATGGATGTTACTTTAGGTAAAGCACTCCCACCAGTCGA
TGGTGTTTGTGCAATGGCACCAAAAGTTTTATCAGGTGAACTTTTCAAAGACCGTAAAGT
TGTTTTATTCGCTGTTCCAGGTGCTTTTACTCCAACTTGTTCAGCCAAACATTTACCAGG
TTTTATTGAAAAATCAGAAGAAATTAAAAAAAAAGGTATCTCTGAAATCTTTTGTATTGC
CACCAATGACCCATTTGTTATGAGTGCTTGGGGTAAAGACGTTAATGCAGGTACTGCAGT
TACTTTACTTTCAGATGGTAACTCTGAATTCACTAAAAAGATTGGACTTGAAATGGATGG
TAAGGCTTTCTTACTTGGTGAAGACAGATCTCAAAGATATGCTATGATCCTTGACTCTGG
TGTTGTCAAACATCTTGCAGTTGAAGAAGGTGGTAAATTTATGTATCATCTGCTAAGCAA
TCTTAAAACAATTATAAACATAAAATCAATCTA
Length of 3' end seq. 563
Connected seq. ID VSD185P
Connected seq. 
>VSD185P.Seq
AAAATATAGAAAATGTCAGGTGCTTTAAAAAATTTAGTTAAATCAGTTATTAAAGTTGGT
GAAAAAGTTCCAATGGATGTTACTTTAGGTAAAGCACTCCCACCAGTCGATGGTGTTTGT
GCAATGGCACCAAAAGTTTTATCAGGTGAACTTTTCAAAGACCGTAAAGTTGTTTTATTC
GCTGTTCCAGGTGCTTTTACTCCAACTTGTTCAGCCAAACATTTACCAGGTTTTATTGAA
AAATCAGAAGAAATTAAAAAAAAAGGTATCTCTGAAATCTTTTGTATTGCCACCAATGAC
CCATTTGTTATGAGTGCTTGGGGTAAAGACGTTAATGCAGGTACTGCAGTTACTTTACTT
TCAGATGGTAACTCTGAATTCACTAAAAAGATTGGACTTGAAATGGATGGTAAGGCTTTC
TTACTTGGTGAAGACAGATCTCAAAGATATGCTATGATCCTTGACTCTGGTGTTGTCAAA
CATCTTGCAGTTGAAGAA----------AAAATATAGAAAATGTCAGGTGCTTTAAAAAA
TTTAGTTAAATCAGTTATTAAAGTTGGTGAAAAAGTTCCAATGGATGTTACTTTAGGTAA
AGCACTCCCACCAGTCGATGGTGTTTGTGCAATGGCACCAAAAGTTTTATCAGGTGAACT
TTTCAAAGACCGTAAAGTTGTTTTATTCGCTGTTCCAGGTGCTTTTACTCCAACTTGTTC
AGCCAAACATTTACCAGGTTTTATTGAAAAATCAGAAGAAATTAAAAAAAAAGGTATCTC
TGAAATCTTTTGTATTGCCACCAATGACCCATTTGTTATGAGTGCTTGGGGTAAAGACGT
TAATGCAGGTACTGCAGTTACTTTACTTTCAGATGGTAACTCTGAATTCACTAAAAAGAT
TGGACTTGAAATGGATGGTAAGGCTTTCTTACTTGGTGAAGACAGATCTCAAAGATATGC
TATGATCCTTGACTCTGGTGTTGTCAAACATCTTGCAGTTGAAGAAGGTGGTAAATTTAT
GTATCATCTGCTAAGCAATCTTAAAACAATTATAAACATAAAATCAATCTA
Length of connected seq. 1061
Full length Seq ID VSD185E
Full length Seq. 
>VSD185E.Seq
AAAATATAGAAAATGTCAGGTGCTTTAAAAAATTTAGTTAAATCAGTTATTAAAGTTGGT
GAAAAAGTTCCAATGGATGTTACTTTAGGTAAAGCACTCCCACCAGTCGATGGTGTTTGT
GCAATGGCACCAAAAGTTTTATCAGGTGAACTTTTCAAAGACCGTAAAGTTGTTTTATTC
GCTGTTCCAGGTGCTTTTACTCCAACTTGTTCAGCCAAACATTTACCAGGTTTTATTGAA
AAATCAGAAGAAATTAAAAAAAAAGGTATCTCTGAAATCTTTTGTATTGCCACCAATGAC
CCATTTGTTATGAGTGCTTGGGGTAAAGACGTTAATGCAGGTACTGCAGTTACTTTACTT
TCAGATGGTAACTCTGAATTCACTAAAAAGATTGGACTTGAAATGGATGGTAAGGCTTTC
TTACTTGGTGAAGACAGATCTCAAAGATATGCTATGATCCTTGACTCTGGTGTTGTCAAA
CATCTTGCAGTTGAAGAAGGTGGTAAATTTATGTATCATCTGCTAAGCAATCTTAAAACA
ATTATAAACATAAAATCAATCTA
Length of full length seq. 563