>VSD172 (VSD172Q) /CSM/VS/VSD1-C/VSD172Q.Seq.d/
CAGGTGGTCCAATGGTAATATGCCACCACAAGGTTTCCCACCAGGTGGTCCAATGGGTAG
AGGTGGTTTCCCTCCACAAGGTTTCCCACCAGGTGGCCCAAACTTCAACAATATGCCACC
ACAAGGTTTCCCACCAGGTGGACCAATGGGTAGAGGTGGTTTCCAAAGAAAATAATAATA
GATAATAAAAAATAAAAAATCTGGAAAAAAAAAAXXXXXXXXXXCAGGTGGTCCAATGGT
AATATGCCACCACAAGGTTTCCCACCAGGTGGTCCAATGGGTAGAGGTGGTTTCCCTCCA
CAAGGTTTCCCACCAGGTGGCCCAAACTTCAACAATATGCCACCACAAGGTTTCCCACCA
GGTGGACCAATGGGTAGAGGNGTTTCCAAAGAAAATAATAATAGNATAATAAAAAATAAA
AA

rwsngnmppqgfppggpmgrggfppqgfppggpnfnnmppqgfppggpmgrggfqrk***
IIKNKKSGKKK---

---RWSNGNMPPQGFPPGGPMGRGGFPPQGFPPGGPNFNNMPPQGFPPGGPMGRGVSKEN
NNXIIKNK

Frame A:
qvvqw*yattrfptrwsng*rwfpstrfptrwpklqqyattrfptrwtng*rwfpkkiii
dnkk*kiwkkk---

---qvvqw*yattrfptrwsng*rwfpstrfptrwpklqqyattrfptrwtng*rxfqrk
***xnkk*k

Frame B:
rwsngnmppqgfppggpmgrggfppqgfppggpnfnnmppqgfppggpmgrggfqrk***
IIKNKKSGKKK---

---RWSNGNMPPQGFPPGGPMGRGGFPPQGFPPGGPNFNNMPPQGFPPGGPMGRGVSKEN
NNXIIKNK

Frame C:
ggpmvichhkvshqvvqwvevvslhkvshqvaqtstichhkvshqvdqwvevvskennnr
**kiknlekk---

---ggpmvichhkvshqvvqwvevvslhkvshqvaqtstichhkvshqvdqwvexfpkki
iix**kik

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSD172 (VSD172Q) /CSM/VS/VSD1-C/VSD172Q.Seq.d/                        369   e-101
SSE303 (SSE303Q) /CSM/SS/SSE3-A/SSE303Q.Seq.d/                        349   2e-95
FCL-AC02 (FCL-AC02Q) /CSM/FCL/FCL-AC/FCL-AC02Q.Seq.d/                 349   2e-95
VSD263 (VSD263Q) /CSM/VS/VSD2-C/VSD263Q.Seq.d/                        125   6e-28
VFJ762 (VFJ762Q) /CSM/VF/VFJ7-C/VFJ762Q.Seq.d/                         86   5e-16
SLE444 (SLE444Q) /CSM/SL/SLE4-B/SLE444Q.Seq.d/                         38   0.10
VSK149 (VSK149Q) /CSM/VS/VSK1-C/VSK149Q.Seq.d/                         36   0.41
VHN596 (VHN596Q) /CSM/VH/VHN5-D/VHN596Q.Seq.d/                         36   0.41
VHB635 (VHB635Q) /CSM/VH/VHB6-B/VHB635Q.Seq.d/                         36   0.41
VSB546 (VSB546Q) /CSM/VS/VSB5-B/VSB546Q.Seq.d/                         34   1.6

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC115680|AC115680.2 Dictyostelium discoideum chromosome 2 map 4415041-4520361 strain AX4, complete sequence.   349  e-104  7
X58063|X58063.1 Brugia pahangi Mf22 gene for a major protein component of the  microfilarial (L1) sheath.    34  1e-05  4
AL440068|AL440068.1 T7 end of clone BD0AA009G07 of library BD0AA from strain CBS 94 of  Candida tropicalis.    26  9e-04  6
AL440241|AL440241.1 T3 end of clone BD0AA010H12 of library BD0AA from strain CBS 94 of  Candida tropicalis.    26  0.001  6
X82314|X82314.1 B.pahangi mRNA for sheath protein.                34  0.071  3
BJ032349|BJ032349.1 Xenopus laevis cDNA clone:XL016g08, 5' end, single read.    36  0.10  3
CD101287|CD101287.1 AGENCOURT_13997107 NICHD_XGC_Tad1 Xenopus laevis cDNA clone IMAGE:6939099 5', mRNA sequence.    36  0.16  3
BQ725787|BQ725787.1 AGENCOURT_8217456 NICHD XGC Emb2 Xenopus laevis cDNA clone IMAGE:4889587 5', mRNA sequence.    36  0.20  3
AC106662|AC106662.3 Rattus norvegicus clone CH230-74A13, *** SEQUENCING IN PROGRESS ***, 19 unordered pieces.    34  0.22  5
AL238985|AL238985.1 Tetraodon nigroviridis genome survey sequence T7 end of clone 016D14 of library G from Tetraodon nigroviridis.    32  0.40  4

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3268448
Number of Hits to DB: 456,601,412

 psg: 0.60  gvh: 0.20  alm: 0.68  top: 0.53  tms: 0.00  mit: 0.09  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial

>> prediction for VSD172 is nuc

>VSD172F.Seq
CAGGTGGTCCAATGGTAATATGCCACCACAAGGTTTCCCACCAGGTGGTCCAATGGGTAG
AGGTGGTTTCCCTCCACAAGGTTTCCCACCAGGTGGCCCAAACTTCAACAATATGCCACC
ACAAGGTTTCCCACCAGGTGGACCAATGGGTAGAGGTGGTTTCCAAAGAAAATAATAATA
GATAATAAAAAATAAAAAATCTGGAAAAAAAAAA----------

>VSD172Z.Seq
----------CAGGTGGTCCAATGGTAATATGCCACCACAAGGTTTCCCACCAGGTGGTC
CAATGGGTAGAGGTGGTTTCCCTCCACAAGGTTTCCCACCAGGTGGCCCAAACTTCAACA
ATATGCCACCACAAGGTTTCCCACCAGGTGGACCAATGGGTAGAGGNGTTTCCAAAGAAA
ATAATAATAGNATAATAAAAAATAAAAA

>VSD172P.Seq
CAGGTGGTCCAATGGTAATATGCCACCACAAGGTTTCCCACCAGGTGGTCCAATGGGTAG
AGGTGGTTTCCCTCCACAAGGTTTCCCACCAGGTGGCCCAAACTTCAACAATATGCCACC
ACAAGGTTTCCCACCAGGTGGACCAATGGGTAGAGGTGGTTTCCAAAGAAAATAATAATA
GATAATAAAAAATAAAAAATCTGGAAAAAAAAAA----------CAGGTGGTCCAATGGT
AATATGCCACCACAAGGTTTCCCACCAGGTGGTCCAATGGGTAGAGGTGGTTTCCCTCCA
CAAGGTTTCCCACCAGGTGGCCCAAACTTCAACAATATGCCACCACAAGGTTTCCCACCA
GGTGGACCAATGGGTAGAGGNGTTTCCAAAGAAAATAATAATAGNATAATAAAAAATAAA
AA