>VSC522 (VSC522Q) /CSM/VS/VSC5-A/VSC522Q.Seq.d/
GACAAATGAAATTATCTTTCATTAAAAACATTTCTAACAATGAAGGGTTTAGGGTTACTA
ATTTTAAAAGGAAAACGGAGGATACAATAATAAATTTAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAA

QMKLSFIKNISNNEGFRVTNFKRKTEDTIINLKKKKKKKKKK

Frame A:
dk*nylslktfltmkglgllilkgkrriq**i*kkkkkkkkkk


Frame B:
tneiifh*khf*q*rv*gy*f*kenggynnkfkkkkkkkkkk


Frame C:
QMKLSFIKNISNNEGFRVTNFKRKTEDTIINLKKKKKKKKKK

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSC522 (VSC522Q) /CSM/VS/VSC5-A/VSC522Q.Seq.d/                        192   8e-49
VSF785 (VSF785Q) /CSM/VS/VSF7-D/VSF785Q.Seq.d/                         32   1.8
VSF301 (VSF301Q) /CSM/VS/VSF3-A/VSF301Q.Seq.d/                         32   1.8
VFN612 (VFN612Q) /CSM/VF/VFN6-A/VFN612Q.Seq.d/                         32   1.8
VFM491 (VFM491Q) /CSM/VF/VFM4-D/VFM491Q.Seq.d/                         32   1.8
VFL748 (VFL748Q) /CSM/VF/VFL7-B/VFL748Q.Seq.d/                         32   1.8
VFF132 (VFF132Q) /CSM/VF/VFF1-B/VFF132Q.Seq.d/                         32   1.8
VFC691 (VFC691Q) /CSM/VF/VFC6-D/VFC691Q.Seq.d/                         32   1.8
VFB573 (VFB573Q) /CSM/VF/VFB5-D/VFB573Q.Seq.d/                         32   1.8
SSM574 (SSM574Q) /CSM/SS/SSM5-D/SSM574Q.Seq.d/                         32   1.8

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AI302988|AI302988.1 qn52g02.x1 NCI_CGAP_Kid5 Homo sapiens cDNA clone IMAGE:1901906 3' similar to TR:Q15662 Q15662 TRANSFORMATION-RELATED PROTEIN ;, mRNA sequence.    46  0.093  1
AC011604|AC011604.10 Homo sapiens 12p12-31.7-37.2 BAC RP11-80N2 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    42  0.11  3
Z14085|Z14085.1 N.tabacum mRNA for ribosomal protein S4 (partial).    44  0.37  1
AV263649|AV263649.1 Mus musculus adult male testis cDNA, RIKEN full-length enriched library, clone:4930439N15, 3' end partial sequence.    44  0.37  1
AC145563|AC145563.1 Mus musculus chromosome UNK clone RP24-75L3, *** SEQUENCING IN PROGRESS ***, 21 unordered pieces.    36  0.54  3
AL357034|AL357034.18 Human DNA sequence from clone RP11-560I21 on chromosome 6.    34  0.77  3
BZ473434|BZ473434.1 BONDK43TF BO_1.6_2_KB_tot Brassica oleracea genomic clone BONDK43, DNA sequence.    36  0.95  2
AZ189131|AZ189131.1 SP_1013_B1_F04_SP6E Strongylocentrotus purpuratus, purple sea urchin, sperm genomic BAC library Strongylocentrotus purpuratus genomic clone Plate=1013 Col=7 Row=L, DNA sequence.    34  1.0  2
BH645191|BH645191.1 BOMGU94TR BO_2_3_KB Brassica oleracea genomic clone BOMGU94, DNA sequence.    36  1.0  2
BY614492|BY614492.1 Mus musculus visual cortex cDNA, RIKEN full-length enriched library, clone:K330004E01, 3' end partial sequence.    42  1.4  1

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3268448
Number of Hits to DB: 131,550,321

 psg: 0.61  gvh: 0.15  alm: 0.64  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.74  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 80.0 %: nuclear
	 16.0 %: cytoplasmic
	  4.0 %: mitochondrial

>> prediction for VSC522 is nuc

>VSC522E.Seq
GACAAATGAAATTATCTTTCATTAAAAACATTTCTAACAATGAAGGGTTTAGGGTTACTA
ATTTTAAAAGGAAAACGGAGGATACAATAATAAATTTAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAA