>VSC521 (VSC521Q) /CSM/VS/VSC5-A/VSC521Q.Seq.d/
CAAGAATATGATTGAAGATATTGGTGAATCAGATAGTCCAATTCCATTACCAAATGTTAC
TAGCACTATTTTAGAGAAAGTTCTTGACTATTGCAGACATCACCATCAACATCCATCACC
ACAAGGTGACGATAAAAAGGATGAAAAGAGATTAGATGATATCCCACCATATGATAGAGA
TTTCTGTAAAGTCGATCAACCAACCTTATTCGAATTAATCTTGGCAGCCAATTATTTGGA
TATCAAACCATTATTAGATGTTACCTGTAAAACTGTTGCCAATATGATCAGAGGTAAAAC
CCCAGAAGAAATCAGAAAAATCTTCAACATCAAGAACGACTTTACTCCAGAAGAAGAAGA
ACAAATCAGAAAAGAAAATGAATGGTGTGAAGATAAAGGTGGAAACTAAACAGTCAAGTA
TCTCTTTTTTTTTTTTTGATATAAAAAAAAAAAAA

KNMIEDIGESDSPIPLPNVTSTILEKVLDYCRHHHQHPSPQGDDKKDEKRLDDIPPYDRD
FCKVDQPTLFELILAANYLDIKPLLDVTCKTVANMIRGKTPEEIRKIFNIKNDFTPEEEE
QIRKENEWCEDKGGN*tvkylffff*ykkkk

Frame A:
qeyd*ryw*ir*snsitkcy*hyfress*llqtspstsittr*r*kg*keir*ypti**r
fl*srstnlirinlgsqlfgyqtiircyl*nccqydqr*nprrnqknlqhqerlysrrrr
tnqkrk*mv*r*rwklnsqvslfffli*kkk


Frame B:
KNMIEDIGESDSPIPLPNVTSTILEKVLDYCRHHHQHPSPQGDDKKDEKRLDDIPPYDRD
FCKVDQPTLFELILAANYLDIKPLLDVTCKTVANMIRGKTPEEIRKIFNIKNDFTPEEEE
QIRKENEWCEDKGGN*tvkylffff*ykkkk


Frame C:
ri*lkilvnqivqfhyqmllalf*rkfltiaditinihhhkvtikrmkrd*mishhmiei
svksinqpysn*swqpiiwisnhy*mlpvkllpi*sevkpqkkseksstsrttllqkkkn
ksekkmngvkikvetkqssisffffdikkkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSC521 (VSC521Q) /CSM/VS/VSC5-A/VSC521Q.Seq.d/                        841   0.0
SSM124 (SSM124Q) /CSM/SS/SSM1-A/SSM124Q.Seq.d/                        841   0.0
SSF605 (SSF605Q) /CSM/SS/SSF6-A/SSF605Q.Seq.d/                        783   0.0
VSB786 (VSB786Q) /CSM/VS/VSB7-D/VSB786Q.Seq.d/                        729   0.0
VSK110 (VSK110Q) /CSM/VS/VSK1-A/VSK110Q.Seq.d/                        599   e-170
VSJ401 (VSJ401Q) /CSM/VS/VSJ4-A/VSJ401Q.Seq.d/                        599   e-170
VSH570 (VSH570Q) /CSM/VS/VSH5-C/VSH570Q.Seq.d/                        599   e-170
SSK557 (SSK557Q) /CSM/SS/SSK5-C/SSK557Q.Seq.d/                        599   e-170
SSI765 (SSI765Q) /CSM/SS/SSI7-C/SSI765Q.Seq.d/                        599   e-170
FC-AW05 (FC-AW05Q) /CSM/FC/FC-AW/FC-AW05Q.Seq.d/                      599   e-170

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U73685|U73685.1 Dictyostelium discoideum cytosolic glycoprotein FP21 (fpa1) gene, complete cds.   841  0.0  1
U18063|U18063.1 Dictyostelium discoideum glycoprotein FP21 mRNA, complete cds.   833  0.0  1
U73686|U73686.1 Dictyostelium discoideum cytosolic glycoprotein FP21 (fpa2) gene, complete cds.   565  e-160  3
AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.   565  e-157  3
CD463492|CD463492.1 ETH1_44_D07.g1_A002 Ethylene-treated seedlings Sorghum bicolor cDNA clone ETH1_44_D07_A002 5', mRNA sequence.    72  5e-23  4
CB073377|CB073377.1 taa32b02.y1 Hydra EST -II Hydra magnipapillata cDNA 5' similar to SW:SKP1_HUMAN P34991 CYCLIN A/CDK2-ASSOCIATED PROTEIN P19 ;, mRNA sequence.    74  4e-18  3
CB272183|CB272183.1 taa32b02.x2 Hydra EST -II Hydra magnipapillata cDNA 3' similar to SW:SKP1_HUMAN P34991 CYCLIN A/CDK2-ASSOCIATED PROTEIN P19 ;, mRNA sequence.    74  5e-18  3
BI503566|BI503566.1 BB170032B10F03.5 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170032B10F03 5', mRNA sequence.    74  1e-17  2
BE844308|BE844308.1 EST052 Apis mellifera female antennae Lambda ZAP II library Apis mellifera cDNA clone pAmfaF62 5' similar to RNA polymerase II elongation factor-like protein of mouse, mRNA sequence.    74  1e-17  2
AI111115|AI111115.1 MF004A08.NK3 S. mansoni, female enriched, subtractive, HHU(A) Schistosoma mansoni cDNA clone MF004A08.NK 5' similar to AA559472, Schistosoma mansoni cDNA clone similar to RNA polymerase II elongation factor-like protein, mRNA sequence.    66  6e-16  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

FB831795_1(FB831795|pid:none) Sequence 51068 from Patent WO20080...   228   4e-59
AY050559_1(AY050559|pid:none) Physarum polycephalum Skp1 (Skp1) ...   218   4e-56
FB831983_1(FB831983|pid:none) Sequence 51256 from Patent WO20080...   217   9e-56
AB170508_1(AB170508|pid:none) Macaca fascicularis brain cDNA clo...   199   3e-50
AK078330_1(AK078330|pid:none) Mus musculus adult male olfactory ...   196   2e-49
BT080079_1(BT080079|pid:none) Esox lucius clone eluc-evq-507-350...   196   3e-49
BT073368_1(BT073368|pid:none) Oncorhynchus mykiss clone omyk-evn...   196   3e-49
(Q6PEC4) RecName: Full=S-phase kinase-associated protein 1; AltN...   196   3e-49
U37558_1(U37558|pid:none) Human RNA polymerase II elongation fac...   196   3e-49
AK166673_1(AK166673|pid:none) Mus musculus 1 cell embryo 1 cell ...   196   3e-49

 psg: 0.75  gvh: 0.28  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 24.0 %: cytoskeletal
	 24.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: mitochondrial
	  4.0 %: vacuolar

>> prediction for VSC521 is cyt

>VSC521E.Seq
CAAGAATATGATTGAAGATATTGGTGAATCAGATAGTCCAATTCCATTACCAAATGTTAC
TAGCACTATTTTAGAGAAAGTTCTTGACTATTGCAGACATCACCATCAACATCCATCACC
ACAAGGTGACGATAAAAAGGATGAAAAGAGATTAGATGATATCCCACCATATGATAGAGA
TTTCTGTAAAGTCGATCAACCAACCTTATTCGAATTAATCTTGGCAGCCAATTATTTGGA
TATCAAACCATTATTAGATGTTACCTGTAAAACTGTTGCCAATATGATCAGAGGTAAAAC
CCCAGAAGAAATCAGAAAAATCTTCAACATCAAGAACGACTTTACTCCAGAAGAAGAAGA
ACAAATCAGAAAAGAAAATGAATGGTGTGAAGATAAAGGTGGAAACTAAACAGTCAAGTA
TCTCTTTTTTTTTTTTTGATATAAAAAAAAAAAAA