>VSC304 (VSC304Q) /CSM/VS/VSC3-A/VSC304Q.Seq.d/
XXXXXXXXXXTCTTCCACCGCCGCTGGCACCACCACCTCCTGATGATGGAGGAGCACTGG
CCATTGGTGGTGGTGGAGGTGGTGGACCACTAGAAGGTGGTGGTGGAGGTGGTGGTGGTG
GTGGAGCACCTCCGCCTGATGGTGGAGCTGCAGGTGTCTTATGGGATGGTGGTGGTGGTG
GTGGAGCACTTTTACCAACAGATGGTGGTGGTGGTGGAGCTGATCTACCTGGTTGGTTTG
AAGATGGTGGAGCTGGTGGAGCTGATCTACCTGGTTGAGTTGGTGGTGGAGCTGAACCAC
CAACCATTTGTTCACCAATGATATTGACAATGAATTGAGCAGTTTCTGCATTCTTTAATT
CACTCTTCTTAATACCAGCAGATTGGAAAAGTTTTCTCCAATCTGGTGGGATATTCTTAA
TATCAAAACCATTCTCTGGATCCCAACCAATATGACTTTCATGTTTAAAGTTGGTTGGAG
CTGAAATTTCCAAATCTTTCTCTTCCGATGAAAAGAATTTAGACATAAAACCTTTCTTTT
CTTTTTTTCTTTTCAACTTTTGGTGCTGGTGTTGATGCAGTAATTTCTATTTAA

---LPPPLAPPPPDDGGALAIGGGGGGGPLEGGGGGGGGGGAPPPDGGAAGVLWDGGGGG
GALLPTDGGGGGADLPGWFEDGGAGGADLPG*vgggaeppticspmiltmn*avsaffns
lflipadwksflqsggifliskpfsgsqpi*lsclklvgaeisksfssdeknldikpffs
fflfnfwcwc*csnfyl

Frame A:
---LPPPLAPPPPDDGGALAIGGGGGGGPLEGGGGGGGGGGAPPPDGGAAGVLWDGGGGG
GALLPTDGGGGGADLPGWFEDGGAGGADLPG*vgggaeppticspmiltmn*avsaffns
lflipadwksflqsggifliskpfsgsqpi*lsclklvgaeisksfssdeknldikpffs
fflfnfwcwc*csnfyl


Frame B:
---fhrrwhhhllmmeehwplvvvevvdh*kvvvevvvvvehlrlmvelqvsygmvvvvv
ehfyqqmvvvveliylvglkmvelveliylvelvvelnhqpfvhq*y*q*ieqflhslih
ss*yqqigkvfsnlvgys*yqnhsldpnqydfhv*swlelkfpnlslpmkri*t*nlsfl
fffstfgagvdavisi*


Frame C:
---staagttts**wrstghwwwwrwwttrrwwwrwwwwwstsa*wwscrclmgwwwwww
stftnrwwwwws*stwlv*rwwswws*stwlswwws*ttnhlftndidnelssfcil*ft
llntsrlekfspiwwdilniktilwiptnmtfmfkvgws*nfqiflfr*kefrhktflff
fsfqllvlvlmq*flf

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSC304 (VSC304Q) /CSM/VS/VSC3-A/VSC304Q.Seq.d/                        622   e-177
SFG565 (SFG565Q) /CSM/SF/SFG5-C/SFG565Q.Seq.d/                        622   e-177
VFN644 (VFN644Q) /CSM/VF/VFN6-B/VFN644Q.Seq.d/                        404   e-112
SHE721 (SHE721Q) /CSM/SH/SHE7-A/SHE721Q.Seq.d/                        341   7e-93
SLH341 (SLH341Q) /CSM/SL/SLH3-B/SLH341Q.Seq.d/                        331   7e-90
AFG687 (AFG687Q) /CSM/AF/AFG6-D/AFG687Q.Seq.d/                        278   9e-74
SFC807 (SFC807Q) /CSM/SF/SFC8-A/SFC807Q.Seq.d/                        230   2e-59
VFD730 (VFD730Q) /CSM/VF/VFD7-B/VFD730Q.Seq.d/                        107   2e-22
VFA863 (VFA863Q) /CSM/VF/VFA8-C/VFA863Q.Seq.d/                        107   2e-22
SSG705 (SSG705Q) /CSM/SS/SSG7-A/SSG705Q.Seq.d/                        107   2e-22

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF305060|AF305060.1 Dictyostelium discoideum Wiscott-Aldrich syndrome protein (wasA) gene, complete cds.   622  0.0  4
AC117072|AC117072.2 Dictyostelium discoideum chromosome 2 map 3323568-3470138 strain AX4, complete sequence.    70  4e-08  2
AC092933|AC092933.21 Homo sapiens 3 BAC RP11-200I19 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    50  0.031  1
BQ258443|BQ258443.1 fz79g06.y1 Sugano SJD adult male Danio rerio cDNA clone 5914930 5' similar to SW:ROA1_HUMAN P09651 HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A1 ;, mRNA sequence.    50  0.031  1
AC131148|AC131148.2 Homo sapiens clone RP11-622J3, *** SEQUENCING IN PROGRESS ***, 6 unordered pieces.    50  0.031  1
AC023415|AC023415.3 Homo sapiens chromosome 3 clone RP11-200I19 map 3, WORKING DRAFT SEQUENCE, 27 unordered pieces.    50  0.086  2
AL260282|AL260282.1 Tetraodon nigroviridis genome survey sequence PUC-Ori end of clone  057L17 of library G from Tetraodon nigroviridis.    48  0.12  1
AL169748|AL169748.1 Tetraodon nigroviridis genome survey sequence T7 end of clone 199F17 of library G from Tetraodon nigroviridis.    48  0.12  1
X04335|X04335.1 Petunia grp-1 gene for glycine-rich protein.      36  0.29  2
AC023677|AC023677.4 Drosophila melanogaster 3L BAC RP98-20I1 (Roswell Park Cancer Institute Drosophila BAC Library) complete sequence.    46  0.49  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF305060_1(AF305060|pid:none) Dictyostelium discoideum Wiscott-A...   167   1e-44
AC117076_18(AC117076|pid:none) Dictyostelium discoideum chromoso...   113   3e-24
(P42768) RecName: Full=Wiskott-Aldrich syndrome protein;        ...    79   1e-13
AX167535_1(AX167535|pid:none) Sequence 35 from Patent WO0144292....    79   1e-13
AF115549_1(AF115549|pid:none) Homo sapiens Wiskott-Aldrich Syndr...    79   1e-13
BC158677_1(BC158677|pid:none) Rattus norvegicus Wiskott-Aldrich ...    79   1e-13
(P70315) RecName: Full=Wiskott-Aldrich syndrome protein homolog;...    79   1e-13
BC087802_1(BC087802|pid:none) Xenopus tropicalis Wiskott-Aldrich...    75   2e-12
BC129739_1(BC129739|pid:none) Xenopus laevis hypothetical protei...    75   2e-12
BC158507_1(BC158507|pid:none) Xenopus tropicalis Wiskott-Aldrich...    75   2e-12

 psg: 0.75  gvh: 0.40  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.16  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: endoplasmic reticulum

>> prediction for VSC304 is cyt

>VSC304Z.Seq
----------TCTTCCACCGCCGCTGGCACCACCACCTCCTGATGATGGAGGAGCACTGG
CCATTGGTGGTGGTGGAGGTGGTGGACCACTAGAAGGTGGTGGTGGAGGTGGTGGTGGTG
GTGGAGCACCTCCGCCTGATGGTGGAGCTGCAGGTGTCTTATGGGATGGTGGTGGTGGTG
GTGGAGCACTTTTACCAACAGATGGTGGTGGTGGTGGAGCTGATCTACCTGGTTGGTTTG
AAGATGGTGGAGCTGGTGGAGCTGATCTACCTGGTTGAGTTGGTGGTGGAGCTGAACCAC
CAACCATTTGTTCACCAATGATATTGACAATGAATTGAGCAGTTTCTGCATTCTTTAATT
CACTCTTCTTAATACCAGCAGATTGGAAAAGTTTTCTCCAATCTGGTGGGATATTCTTAA
TATCAAAACCATTCTCTGGATCCCAACCAATATGACTTTCATGTTTAAAGTTGGTTGGAG
CTGAAATTTCCAAATCTTTCTCTTCCGATGAAAAGAATTTAGACATAAAACCTTTCTTTT
CTTTTTTTCTTTTCAACTTTTGGTGCTGGTGTTGATGCAGTAATTTCTATTTAA