>VSC236 (VSC236Q) /CSM/VS/VSC2-B/VSC236Q.Seq.d/
AAAATGCCAACCAGATTCAGTAAGCATGGAAAATCAAGAGGTGACGTTTGTGCCGGTTAC
GGTCGTGTTGGTAAACACAGAAAGCATCCAGGTGGTCGTGGTAATGCTGGTGGTCTCACT
CATCACAGAATTAACTTTGACAAATACCATCCAGGTTATTTCGGTAAATTAGGTATGAGA
CATTTCCATTTATTAAGAAACCAATACCATTGTCCAACCGTCTCATTAGAAAAGATCTGG
ACTTTAGTCCCAGAATCCGTCAGAAAATCATTAGCTGCCAAGAATGACGGTACTGCTCCA
GTTGTTGATGTCACCCAAAAAGGTTTCTTCAAAGTTTTAGGTCACGGTATCCTCCCAACT
CAACCAATCATCGTCAAAGCTCGTTATTTCTCTAAAGTCGCTGAAAAGAAAATCAAAGCT
GTCGGTGGTGCTTGTATCTTAGTTGCTTAAATATTTTATACGAATCAATAAATATAAATA
AAAAAAGTAATTAAATTTAAAAAATTTAAAAAAAAAAA

KMPTRFSKHGKSRGDVCAGYGRVGKHRKHPGGRGNAGGLTHHRINFDKYHPGYFGKLGMR
HFHLLRNQYHCPTVSLEKIWTLVPESVRKSLAAKNDGTAPVVDVTQKGFFKVLGHGILPT
QPIIVKARYFSKVAEKKIKAVGGACILVA*ifytnq*i*ikkvikfkkfkkk

Frame A:
KMPTRFSKHGKSRGDVCAGYGRVGKHRKHPGGRGNAGGLTHHRINFDKYHPGYFGKLGMR
HFHLLRNQYHCPTVSLEKIWTLVPESVRKSLAAKNDGTAPVVDVTQKGFFKVLGHGILPT
QPIIVKARYFSKVAEKKIKAVGGACILVA*ifytnq*i*ikkvikfkkfkkk


Frame B:
kcqpdsvsmenqevtfvpvtvvlvntesiqvvvvmlvvsliteltltntiqvisvn*v*d
isiy*etntivqpsh*krsgl*sqnpsenh*lprmtvllqllmspkkvsskf*vtvssql
nqsssklvislkslkrksklsvvlvs*llkyfirinkyk*kk*lnlknlkkk


Frame C:
nanqiq*awkikr*rlcrlrscw*tqkasrwsw*cwwshssqn*l*qipsrlfr*iryet
fpfikkpiplsnrlirkdldfsprirqkiiscqe*rycssc*chpkrflqsfrsryppns
tnhrqsslfl*sr*kenqscrwclylsclnilyesininkksn*i*ki*kkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSC236 (VSC236Q) /CSM/VS/VSC2-B/VSC236Q.Seq.d/                        922   0.0
VSJ737 (VSJ737Q) /CSM/VS/VSJ7-B/VSJ737Q.Seq.d/                        914   0.0
VSJ618 (VSJ618Q) /CSM/VS/VSJ6-A/VSJ618Q.Seq.d/                        914   0.0
VSI571 (VSI571Q) /CSM/VS/VSI5-C/VSI571Q.Seq.d/                        914   0.0
VSI537 (VSI537Q) /CSM/VS/VSI5-B/VSI537Q.Seq.d/                        914   0.0
VSH826 (VSH826Q) /CSM/VS/VSH8-B/VSH826Q.Seq.d/                        914   0.0
VSH782 (VSH782Q) /CSM/VS/VSH7-D/VSH782Q.Seq.d/                        914   0.0
VSG825 (VSG825Q) /CSM/VS/VSG8-B/VSG825Q.Seq.d/                        914   0.0
VSG638 (VSG638Q) /CSM/VS/VSG6-B/VSG638Q.Seq.d/                        914   0.0
VSF775 (VSF775Q) /CSM/VS/VSF7-D/VSF775Q.Seq.d/                        914   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

D50338|D50338.1 Dictyostelium discoideum DNA for ribosomal protein, complete cds.   904  0.0  1
BI863538|BI863538.1 kx45d11.y1 Parastrongyloides trichosuri FL pAMP1 v1 Chiapelli McCarter Parastrongyloides trichosuri cDNA 5' similar to SW:RL2A_OSCBR O01358 60S RIBOSOMAL PROTEIN L27A ;, mRNA sequence.    82  1e-26  3
BP014972|BP014972.1 Ciona intestinalis cDNA, clone:ciad63m07, 5' end, single read.    72  2e-25  4
BP010592|BP010592.1 Ciona intestinalis cDNA, clone:ciad74n20, 5' end, single read.    72  7e-25  4
BW126894|BW126894.1 Ciona intestinalis cDNA, clone:rcign012m06, 3' end, single read.    72  9e-25  4
BW094561|BW094561.1 Ciona intestinalis cDNA, clone:rcitb049o03, 3' end, single read.    72  9e-25  4
BW124755|BW124755.1 Ciona intestinalis cDNA, clone:rciad100o10, 3' end, single read.    72  9e-25  4
BM961080|BM961080.1 cihA11A16T Ascidian hemocytes cDNA library Ciona intestinalis cDNA, mRNA sequence.    72  9e-25  4
BP005921|BP005921.1 Ciona intestinalis cDNA, clone:ciad39g13, 5' end, single read.    72  9e-25  4
AV986190|AV986190.1 Ciona intestinalis cDNA, clone:cilv41c03, 5' end, single read.    72  9e-25  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P48160) RecName: Full=60S ribosomal protein L27a;  &D50338_1(D5...   310   1e-83
BT046766_1(BT046766|pid:none) Salmo salar clone ssal-eve-548-138...   224   1e-57
AY961477_1(AY961477|pid:none) Phytophthora infestans clone MY-19...   223   2e-57
AF502247_1(AF502247|pid:none) Epinephelus coioides ribosomal pro...   220   2e-56
AJ843085_1(AJ843085|pid:none) Platichthys flesus mRNA for 60S ri...   220   2e-56
(P18445) RecName: Full=60S ribosomal protein L27a;  &R5RTLA(S130...   219   2e-56
AB374966_1(AB374966|pid:none) Solea senegalensis RPL27a mRNA for...   219   4e-56
(P46776) RecName: Full=60S ribosomal protein L27a;  &(Q5REY2) Re...   219   4e-56
AJ307670_3(AJ307670|pid:none) Mus musculus St5 gene, L27a gene a...   219   4e-56
AB172987_1(AB172987|pid:none) Macaca fascicularis brain cDNA clo...   218   9e-56

 psg: 0.62  gvh: 0.20  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.12
 nuc: 0.02  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.50  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 20.0 %: mitochondrial
	 20.0 %: nuclear

>> prediction for VSC236 is cyt

>VSC236E.Seq
AAAATGCCAACCAGATTCAGTAAGCATGGAAAATCAAGAGGTGACGTTTGTGCCGGTTAC
GGTCGTGTTGGTAAACACAGAAAGCATCCAGGTGGTCGTGGTAATGCTGGTGGTCTCACT
CATCACAGAATTAACTTTGACAAATACCATCCAGGTTATTTCGGTAAATTAGGTATGAGA
CATTTCCATTTATTAAGAAACCAATACCATTGTCCAACCGTCTCATTAGAAAAGATCTGG
ACTTTAGTCCCAGAATCCGTCAGAAAATCATTAGCTGCCAAGAATGACGGTACTGCTCCA
GTTGTTGATGTCACCCAAAAAGGTTTCTTCAAAGTTTTAGGTCACGGTATCCTCCCAACT
CAACCAATCATCGTCAAAGCTCGTTATTTCTCTAAAGTCGCTGAAAAGAAAATCAAAGCT
GTCGGTGGTGCTTGTATCTTAGTTGCTTAAATATTTTATACGAATCAATAAATATAAATA
AAAAAAGTAATTAAATTTAAAAAATTTAAAAAAAAAAA