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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AHA238
Library AH
(Link to library)
Clone ID AHA238
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11101-1
Original site URL
Representative seq. ID AHA238P
(Link to Original site)
Representative DNA sequence 
>AHA238 (AHA238Q) /CSM/AH/AHA2-B/AHA238Q.Seq.d/
ATTCCAGAATTAATCTTTGGTAATCTCTTAACAAGTAGCCATTATGATGACTCTGAAAAG
AGATTAACTGGTGGTCGTAATGGTTTCGGTGCAAAGTTGGCCAATATTTTCAGTACTAAA
TTCATTGTAGAGTGTGCCGATTCAAAGTCAAAGAAATTGTATAAACAAACCTTCACTAAT
AACATGCGTTCCAAAGAGGATCCAAAGATCACCTCATATCAAAATAAAACCGATTATACA
AAGATTACCTTTTATCCAGAACTCGATCGTTTTGGTATGGTTGAATTTGATGATGATTTG
GTTGCATTACTCTCCAAACGTGTTTATGATATTGCCGGTTGTAATCCAACACTTAAAGTT
TCCCTCAACGACGAAGAGTTGGGTATTAGATCCTTTGAAAAATATATTAATCTCTATTTT
CCAGATGAAGAGAATGCACCAAAAGTTTACTATGAAAAAGTTAGTGATAGATGGGAACTT
GCTGTCACTCTCTCAAAGGAGAGTCAATTCAATCAAGTTAGTTTTGTAAATTCAATTTGT
ACAGTCAAAGGTGGTACACATGTAACTCATGCTCTTAGTAATATCGTTACCGCCATTCAA
GAXXXXXXXXXXGGTCAAGTACAATTCCAAATTATAATCCAAGAGATTTAATTCAAAATA
TGAGATTAGCAATCGATGGTAAACCATTGAAACCAATCAAACCATGGTATAGAGGTTTCC
TTGGTTCCATCGAAGCAAATCAAGGTTCCAAAGTACAAGGTGGTCAATATCTTTCCAAAG
GTATTTGGAAAAAATTATCCGATAATAGATTTGAAATCACAGAGTTACCAATTGGTTTTT
GGACTCAAGATTATAGAGAACTCTTGGATGAATTAGAAACTCCAGGTACTAGAAAGAAAA
GGAAAGAAGAGAAAGAAAAGAAGGCTGCTAGTAGAAAAGGTACTAAAGCAAAACCAACCA
CTACAAAGAGATCAAAGAGAGTCGATGATGATGATGATAATGAAAAAGTCACTGAAGCAA
CCGTTAAATCATATACCAACTACTCCTCTGAATCAACTATTCATTTCATTATTGACACCA
TTCAACCAGTCGATGAAATTAACATTGAAAAAGTTTTTAAACTCGTTTCCACAATTAATG
AAACCAATATGGTAGTATTCGATGAAGAAGGTCGTATTCAAAGATTTGCAACCACCACTG
CCTTACAAGAACATTTCTTCCCATTAAGAATCAAACACTATCAAATGAGAAAAGATTTCC
TCTCTGAGCGTCTCTCTGAAGAATATTCTCGTCTTTCAAATAAAGCTCGTTTCATTTTAG
CCGTTGTAAATAAGGAATTGGTAATTTCAAATGTTAAAAAAGTGATCTCATTAACAAATT
AAAGGAA
sequence update 2002.10.25
Translated Amino Acid sequence 
IPELIFGNLLTSSHYDDSEKRLTGGRNGFGAKLANIFSTKFIVECADSKSKKLYKQTFTN
NMRSKEDPKITSYQNKTDYTKITFYPELDRFGMVEFDDDLVALLSKRVYDIAGCNPTLKV
SLNDEELGIRSFEKYINLYFPDEENAPKVYYEKVSDRWELAVTLSKESQFNQVSFVNSIC
TVKGGTHVTHALSNIVTAIQ---

---SSTIPNYNPRDLIQNMRLAIDGKPLKPIKPWYRGFLGSIEANQGSKVQGGQYLSKGI
WKKLSDNRFEITELPIGFWTQDYRELLDELETPGTRKKRKEEKEKKAASRKGTKAKPTTT
KRSKRVDDDDDNEKVTEATVKSYTNYSSESTIHFIIDTIQPVDEINIEKVFKLVSTINET
NMVVFDEEGRIQRFATTTALQEHFFPLRIKHYQMRKDFLSERLSEEYSRLSNKARFILAV
VNKELVISNVKKVISLTN*r
Translated Amino Acid sequence (All Frames) 
Frame A:
IPELIFGNLLTSSHYDDSEKRLTGGRNGFGAKLANIFSTKFIVECADSKSKKLYKQTFTN
NMRSKEDPKITSYQNKTDYTKITFYPELDRFGMVEFDDDLVALLSKRVYDIAGCNPTLKV
SLNDEELGIRSFEKYINLYFPDEENAPKVYYEKVSDRWELAVTLSKESQFNQVSFVNSIC
TVKGGTHVTHALSNIVTAIQ---

---gqvqfqiiiqei*fki*d*qsmvnh*nqsnhgievslvpskqikvpkykvvnifpkv
fgknypiidlksqsyqlvfglkiienswmn*klqvlerkgkkrkkrrllvekvlkqnqpl
qrdqresmmmmimkkslkqplnhipttplnqlfislltpfnqsmkltlkkflnsfpqlmk
piw*ysmkkvvfkdlqpplpyknissh*esntik*ekisslsvslknilvfqiklvsf*p
l*irnw*fqmlkk*sh*qikg

Frame B:
fqn*slvis*qvaimmtlkrd*lvvvmvsvqswpifsvlnsl*svpiqsqrncinkpsli
tcvpkriqrsphikikpiiqrlpfiqnsivlvwlnlmmiwlhyspnvfmilpvviqhlkf
psttkswvldplknilisifqmkrmhqkftmkklvidgnllslsqrrvnsiklvl*iqfv
qskvvhm*lmllvislppfk---

---vkynskl*skrfnskyeisnrw*tietnqtmv*rfpwfhrsksrfqstrwsisfqry
lekiir**i*nhrvtnwfldsrl*rtlg*irnsry*kekerrerkegc**kry*sktnhy
keikesr******ksh*snr*iiyqlll*inysfhy*hhstsr*n*h*ksf*trfhn**n
qygsir*rrsyskicnhhcltrtflpiknqtlsnekrfpl*asl*rifssfk*ssfhfsr
ck*gignfkc*ksdlinklke

Frame C:
srinlw*slnk*pl**l*keinwws*wfrckvgqyfqy*ihcrvcrfkvkeiv*tnlh**
hafqrgskdhlisk*nrlykdyllsrtrsfwyg*i***fgcitlqtcl*ycrl*snt*sf
pqrrrvgy*il*kiy*slfsr*rectksll*ks***mgtcchslkgesiqss*fckfnly
sqrwytcnscs**yryrhsr---

---SSTIPNYNPRDLIQNMRLAIDGKPLKPIKPWYRGFLGSIEANQGSKVQGGQYLSKGI
WKKLSDNRFEITELPIGFWTQDYRELLDELETPGTRKKRKEEKEKKAASRKGTKAKPTTT
KRSKRVDDDDDNEKVTEATVKSYTNYSSESTIHFIIDTIQPVDEINIEKVFKLVSTINET
NMVVFDEEGRIQRFATTTALQEHFFPLRIKHYQMRKDFLSERLSEEYSRLSNKARFILAV
VNKELVISNVKKVISLTN*r
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AHA238 (AHA238Q) /CSM/AH/AHA2-B/AHA238Q.Seq.d/                       1705   0.0
AHQ518 (AHQ518Q) /CSM/AH/AHQ5-A/AHQ518Q.Seq.d/                        557   e-157
AHJ764 (AHJ764Q) /CSM/AH/AHJ7-C/AHJ764Q.Seq.d/                        480   e-134
AHG769 (AHG769Q) /CSM/AH/AHG7-C/AHG769Q.Seq.d/                        456   e-127
AHN188 (AHN188Q) /CSM/AH/AHN1-D/AHN188Q.Seq.d/                        379   e-104
AHE655 (AHE655Q) /CSM/AH/AHE6-C/AHE655Q.Seq.d/                        337   3e-91
SHD495 (SHD495Q) /CSM/SH/SHD4-D/SHD495Q.Seq.d/                        145   2e-33
SHG606 (SHG606Q) /CSM/SH/SHG6-A/SHG606Q.Seq.d/                        141   3e-32
VHH591 (VHH591Q) /CSM/VH/VHH5-D/VHH591Q.Seq.d/                         46   0.001
VSD338 (VSD338Q) /CSM/VS/VSD3-B/VSD338Q.Seq.d/                         42   0.022
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

D82024|D82024.1 Dictyostelium discoideum DNA for DNA topoisomerase II, complete cds.    50   5e-08  5
BD160804|BD160804.1 Method for identifying and detecting Candida yeast with II type topoisomerase gene as indication.    44   1e-06  3
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    46   5e-06  20
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    38   3e-05  16
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    40   8e-05  19
AL844505|AL844505.1 Plasmodium falciparum chromosome  6.           38   5e-04  20
AC116330|AC116330.2 Dictyostelium discoideum chromosome 2 map 3191214-3323468 strain AX4, complete sequence.    34   0.002  15
AC117176|AC117176.2 Dictyostelium discoideum chromosome 2 map 5018074-5200947 strain AX4, complete sequence.    30   0.002  13
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    38   0.002  16
BD160798|BD160798.1 Method for identifying and detecting Candida yeast with II type topoisomerase gene as indication.    52   0.003  2
dna update 2004. 2.22
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55BP5) RecName: Full=Probable DNA topoisomerase 2;          EC...   434   e-120
X04326_1(X04326|pid:none) Fission yeast TOP2 gene for DNA topois...   227   8e-58
AL590444_34(AL590444|pid:none) chromosome IV of strain GB-M1 of ...   226   2e-57
(P30182) RecName: Full=DNA topoisomerase 2;          EC=5.99.1.3...   219   2e-55
AB049143_1(AB049143|pid:none) Candida parapsilosis top2 gene for...   211   5e-53
AB049144_1(AB049144|pid:none) Candida parapsilosis top2 gene for...   209   1e-52
AE016818_749(AE016818|pid:none) Ashbya gossypii (= Eremothecium ...   209   2e-52
AB113358_1(AB113358|pid:none) Coprinopsis cinerea topII mRNA for...   208   4e-52
AM167520_1(AM167520|pid:none) Malus x domestica transposon gene ...   207   9e-52
(O93794) RecName: Full=DNA topoisomerase 2;          EC=5.99.1.3...   206   2e-51
protein update 2009. 4.26
PSORT 

 psg: 0.75  gvh: 0.38  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.26  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: nuclear
	 24.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for AHA238 is nuc
5' end seq. ID AHA238F
5' end seq. 
>AHA238F.Seq
ATTCCAGAATTAATCTTTGGTAATCTCTTAACAAGTAGCCATTATGATGACTCTGAAAAG
AGATTAACTGGTGGTCGTAATGGTTTCGGTGCAAAGTTGGCCAATATTTTCAGTACTAAA
TTCATTGTAGAGTGTGCCGATTCAAAGTCAAAGAAATTGTATAAACAAACCTTCACTAAT
AACATGCGTTCCAAAGAGGATCCAAAGATCACCTCATATCAAAATAAAACCGATTATACA
AAGATTACCTTTTATCCAGAACTCGATCGTTTTGGTATGGTTGAATTTGATGATGATTTG
GTTGCATTACTCTCCAAACGTGTTTATGATATTGCCGGTTGTAATCCAACACTTAAAGTT
TCCCTCAACGACGAAGAGTTGGGTATTAGATCCTTTGAAAAATATATTAATCTCTATTTT
CCAGATGAAGAGAATGCACCAAAAGTTTACTATGAAAAAGTTAGTGATAGATGGGAACTT
GCTGTCACTCTCTCAAAGGAGAGTCAATTCAATCAAGTTAGTTTTGTAAATTCAATTTGT
ACAGTCAAAGGTGGTACACATGTAACTCATGCTCTTAGTAATATCGTTACCGCCATTCAA
GANNNNNNNNNN
Length of 5' end seq. 612
3' end seq. ID AHA238Z
3' end seq. 
>AHA238Z.Seq
NNNNNNNNNNGGTCAAGTACAATTCCAAATTATAATCCAAGAGATTTAATTCAAAATATG
AGATTAGCAATCGATGGTAAACCATTGAAACCAATCAAACCATGGTATAGAGGTTTCCTT
GGTTCCATCGAAGCAAATCAAGGTTCCAAAGTACAAGGTGGTCAATATCTTTCCAAAGGT
ATTTGGAAAAAATTATCCGATAATAGATTTGAAATCACAGAGTTACCAATTGGTTTTTGG
ACTCAAGATTATAGAGAACTCTTGGATGAATTAGAAACTCCAGGTACTAGAAAGAAAAGG
AAAGAAGAGAAAGAAAAGAAGGCTGCTAGTAGAAAAGGTACTAAAGCAAAACCAACCACT
ACAAAGAGATCAAAGAGAGTCGATGATGATGATGATAATGAAAAAGTCACTGAAGCAACC
GTTAAATCATATACCAACTACTCCTCTGAATCAACTATTCATTTCATTATTGACACCATT
CAACCAGTCGATGAAATTAACATTGAAAAAGTTTTTAAACTCGTTTCCACAATTAATGAA
ACCAATATGGTAGTATTCGATGAAGAAGGTCGTATTCAAAGATTTGCAACCACCACTGCC
TTACAAGAACATTTCTTCCCATTAAGAATCAAACACTATCAAATGAGAAAAGATTTCCTC
TCTGAGCGTCTCTCTGAAGAATATTCTCGTCTTTCAAATAAAGCTCGTTTCATTTTAGCC
GTTGTAAATAAGGAATTGGTAATTTCAAATGTTAAAAAAGTGATCTCATTAACAAATTAA
AGGAA
Length of 3' end seq. 785
Connected seq. ID AHA238P
Connected seq. 
>AHA238P.Seq
ATTCCAGAATTAATCTTTGGTAATCTCTTAACAAGTAGCCATTATGATGACTCTGAAAAG
AGATTAACTGGTGGTCGTAATGGTTTCGGTGCAAAGTTGGCCAATATTTTCAGTACTAAA
TTCATTGTAGAGTGTGCCGATTCAAAGTCAAAGAAATTGTATAAACAAACCTTCACTAAT
AACATGCGTTCCAAAGAGGATCCAAAGATCACCTCATATCAAAATAAAACCGATTATACA
AAGATTACCTTTTATCCAGAACTCGATCGTTTTGGTATGGTTGAATTTGATGATGATTTG
GTTGCATTACTCTCCAAACGTGTTTATGATATTGCCGGTTGTAATCCAACACTTAAAGTT
TCCCTCAACGACGAAGAGTTGGGTATTAGATCCTTTGAAAAATATATTAATCTCTATTTT
CCAGATGAAGAGAATGCACCAAAAGTTTACTATGAAAAAGTTAGTGATAGATGGGAACTT
GCTGTCACTCTCTCAAAGGAGAGTCAATTCAATCAAGTTAGTTTTGTAAATTCAATTTGT
ACAGTCAAAGGTGGTACACATGTAACTCATGCTCTTAGTAATATCGTTACCGCCATTCAA
GA----------GGTCAAGTACAATTCCAAATTATAATCCAAGAGATTTAATTCAAAATA
TGAGATTAGCAATCGATGGTAAACCATTGAAACCAATCAAACCATGGTATAGAGGTTTCC
TTGGTTCCATCGAAGCAAATCAAGGTTCCAAAGTACAAGGTGGTCAATATCTTTCCAAAG
GTATTTGGAAAAAATTATCCGATAATAGATTTGAAATCACAGAGTTACCAATTGGTTTTT
GGACTCAAGATTATAGAGAACTCTTGGATGAATTAGAAACTCCAGGTACTAGAAAGAAAA
GGAAAGAAGAGAAAGAAAAGAAGGCTGCTAGTAGAAAAGGTACTAAAGCAAAACCAACCA
CTACAAAGAGATCAAAGAGAGTCGATGATGATGATGATAATGAAAAAGTCACTGAAGCAA
CCGTTAAATCATATACCAACTACTCCTCTGAATCAACTATTCATTTCATTATTGACACCA
TTCAACCAGTCGATGAAATTAACATTGAAAAAGTTTTTAAACTCGTTTCCACAATTAATG
AAACCAATATGGTAGTATTCGATGAAGAAGGTCGTATTCAAAGATTTGCAACCACCACTG
CCTTACAAGAACATTTCTTCCCATTAAGAATCAAACACTATCAAATGAGAAAAGATTTCC
TCTCTGAGCGTCTCTCTGAAGAATATTCTCGTCTTTCAAATAAAGCTCGTTTCATTTTAG
CCGTTGTAAATAAGGAATTGGTAATTTCAAATGTTAAAAAAGTGATCTCATTAACAAATT
AAAGGAA
Length of connected seq. 1377
Full length Seq ID -
Full length Seq. -
Length of full length seq. -