>AHA225 (AHA225Q) /CSM/AH/AHA2-B/AHA225Q.Seq.d/
XXXXXXXXXXTCAAAGAGATTGCCACTGAGACCTTGTATGGNATCATCAANTGGTAACAC
ATGAATACGTTTGCCATATTTAACATNTGNNCATTGGTGAACTGAAATCATGTCNCCTAA
TATAACACGAAGATTTATTTCTTACNNCTTTATTCATACGGATCTTNGATGGATCGATGG
TTGGATCTGACAATACAANACAAACGGTATCTCTTCTCTTTNTATCTTTAATTAAAAGTG
TGTCACCTCTAAAGAATTGTAATTGATCCATTGTCTNTGGATTTAATGTAACTACTGAAT
TATCATCATTAAGGGCTTNTTCTACAAATAATCTATCCGGAGCTTTTTTACGTT

---qrdch*dlvwxhqxvtheyvchi*hxxigelkscxli*HEDLFLTXLFIRIXDGSMV
GSDNTXQTVSLLFXSLIKSVSPLKNCN*sivxgfnvttelsslraxstnnlsgaflr

Frame A:
---qrdch*dlvwxhqxvtheyvchi*hxxigelkscxli*HEDLFLTXLFIRIXDGSMV
GSDNTXQTVSLLFXSLIKSVSPLKNCN*sivxgfnvttelsslraxstnnlsgaflr


Frame B:
---keiatetlygiixw*hmntfaifnxxxlvn*nhvx*yntkiyflxlysygsxmdrwl
dltiqxkrylfsxyl*lkvchl*rividplsxdlm*llnyhh*glxlqiiypelfyv


Frame C:
---krlplrpcmxssxgnt*irlpyltxxhw*teimspnitrrfisyxfihtdlxwidgw
i*qyxtngisslxifn*kcvtskel*lihclwi*cny*iiiikgxfyk*sirsfft

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AHA225 (AHA225Q) /CSM/AH/AHA2-B/AHA225Q.Seq.d/                        605   e-172
AHA389 (AHA389Q) /CSM/AH/AHA3-D/AHA389Q.Seq.d/                        543   e-154
VHM874 (VHM874Q) /CSM/VH/VHM8-D/VHM874Q.Seq.d/                        533   e-151
VHL714 (VHL714Q) /CSM/VH/VHL7-A/VHL714Q.Seq.d/                        533   e-151
VHK268 (VHK268Q) /CSM/VH/VHK2-C/VHK268Q.Seq.d/                        533   e-151
VHJ543 (VHJ543Q) /CSM/VH/VHJ5-B/VHJ543Q.Seq.d/                        533   e-151
VHJ302 (VHJ302Q) /CSM/VH/VHJ3-A/VHJ302Q.Seq.d/                        533   e-151
VHH667 (VHH667Q) /CSM/VH/VHH6-C/VHH667Q.Seq.d/                        533   e-151

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U83085|U83085.2 Dictyostelium discoideum cell division cycle protein 48 (cdcD) mRNA, complete cds.   355   e-147  2
AZ525160|AZ525160.1 240PbH01 Pb MBN #21 Plasmodium berghei genomic 3', DNA sequence.    52   7e-09  2
BF298766|BF298766.1 021PbB08 Pb cDNA #20, Charles Yowell and Jane Carlton Plasmodium berghei cDNA 5', mRNA sequence.    44   1e-06  2
BI504030|BI504030.1 BB170028B20C09.5 Bee Brain Normalized/Subtracted Library, BB17 Apis mellifera cDNA clone BB170028B20C09 5', mRNA sequence.    54   0.001  1
BU303842|BU303842.1 603736375F1 CSEQCHN56 Gallus gallus cDNA clone ChEST626f19 5', mRNA sequence.    48   0.078  1
BI394024| similar to valosin-containing protein (H. sapiens) [Homo sapiens], mRNA sequence.    48   0.078  1
AJ448992|AJ448992.1 Gallus gallus EST, clone library riken1, clone 20i11r1.    48   0.078  1
AL844505|AL844505.1 Plasmodium falciparum chromosome  6.           36   0.15   2
T18244|T18244.1 0902c3 czapPFDd2.1, Debopam Chakrabarti Plasmodium falciparum cDNA clone 0902c 5', mRNA sequence.    36   0.23   2
BU006439|BU006439.1 QGH11B13.yg.ab1 QG_EFGHJ lettuce serriola Lactuca sativa cDNA clone QGH11B13, mRNA sequence.    46   0.31   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U83085_1(U83085|pid:none) Dictyostelium discoideum cell division...   129   6e-44
DQ515925_1(DQ515925|pid:none) Nicotiana tabacum putative spindle...   100   9e-34
AL162751_7(AL162751|pid:none) Arabidopsis thaliana DNA chromosom...    98   6e-33
FB906007_1(FB906007|pid:none) Sequence 125280 from Patent WO2008...    97   1e-32
EU606206_1(EU606206|pid:none) Dimocarpus longan cell division cy...    97   1e-32
FB905989_1(FB905989|pid:none) Sequence 125262 from Patent WO2008...    96   2e-32
FB781564_1(FB781564|pid:none) Sequence 837 from Patent WO2008034...    96   2e-32
FB781494_1(FB781494|pid:none) Sequence 767 from Patent WO2008034...    96   2e-32
FB781796_1(FB781796|pid:none) Sequence 1069 from Patent WO200803...    96   2e-32
FB906005_1(FB906005|pid:none) Sequence 125278 from Patent WO2008...    96   3e-32

 psg: 0.75  gvh: 0.60  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 24.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for AHA225 is cyt

>AHA225Z.Seq
NNNNNNNNNNTCAAAGAGATTGCCACTGAGACCTTGTATGGNATCATCAANTGGTAACAC
ATGAATACGTTTGCCATATTTAACATNTGNNCATTGGTGAACTGAAATCATGTCNCCTAA
TATAACACGAAGATTTATTTCTTACNNCTTTATTCATACGGATCTTNGATGGATCGATGG
TTGGATCTGACAATACAANACAAACGGTATCTCTTCTCTTTNTATCTTTAATTAAAAGTG
TGTCACCTCTAAAGAATTGTAATTGATCCATTGTCTNTGGATTTAATGTAACTACTGAAT
TATCATCATTAAGGGCTTNTTCTACAAATAATCTATCCGGAGCTTTTTTACGTT