NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFB774
Library AF
(Link to library)
Clone ID AFB774
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFB774P
(Link to Original site)
Representative DNA sequence 
>AFB774 (AFB774Q) /CSM/AF/AFB7-D/AFB774Q.Seq.d/
ATTTTATTTTCNATTGATAAAATTAAATTAAATAAAATGTCATTAAATATTGAAAAAGTT
AAAGAATTTAGTAATAAAGCATGGGATGAAAATATTTTACCAACACTTTCAAAATATATT
GAAATTCCAAATCAATCACCATTATATGATGCAGAATGGGCAACCAATGGTTATACAGAA
GAAGCAATTCAATTATTAGAGAAATGGATCTTAGCACAAGGTATCAAAGGTATTTCATGT
GAAATTAAAAGAATTGAAGGATTAACACCAATTATTTTCATGGTAGTCGAAGCAACCAAA
ACTGAAAAAGTTAAAACTGTACTTTTATATGGTCACATGGATAAACAACCACCACTCACT
GATGCATGGGATGAAGGATTACATCCATACAAGGCAGTCATCAAGAATAACAAACTCTAT
GGTCGTGGTGGTGCAGATGATGGTTATTCAACATTTGCTTCAATCTCTGCAATCAAAGCA
TTGCAAGAACAATCAATTCCACATGATCGTTATGTAATTATCATTGAAGGTTCCGAAGAA
TXXXXXXXXXXATCTATGATGAATTCTCATGGTACAAAAATACCACTCCAGTCACTAAAG
ATTTAACCCAATTGATGATCAACAAAACTTGGTATCCAACTATTTGCATCACCGGTGCTG
AAGGTATTCCACACACTTCAAATGCTGGTAATGTCATGAGAACTCACACCACCGTTAAAG
TTAGTGTTCGTTTACCACCATCATTCAAAGCAGCTGATGCATCAACTGTATTAAAAGAAA
TTTTCGAAACTAATCCACCATATGGTGCTGAAGTTACCTTCAAAATGGATAAATGTGCTT
CAGGTTGGGATTCACCACAAACTAGACCAGAATTAGAAAAAGCATTAGGAGAAGCTCCAA
ATCTTTTCTTTGGCAAAGACCACGTATACATGGGTGAAGGTGGTTCCATCCCATTCATGA
AAATGTTACATGACCGTTTCAATTGTCAATTTATCGTTACTGGTTTATTAGGTCCAGGTT
CAAATGCTCATGGTCCAAATNAATTCTTAGATATTCCATTTGCCAAGAAATTATTAAGTT
GTGTATTATTATTAGATGCTCATTCCAAGTTNATTAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
ILFSIDKIKLNKMSLNIEKVKEFSNKAWDENILPTLSKYIEIPNQSPLYDAEWATNGYTE
EAIQLLEKWILAQGIKGISCEIKRIEGLTPIIFMVVEATKTEKVKTVLLYGHMDKQPPLT
DAWDEGLHPYKAVIKNNKLYGRGGADDGYSTFASISAIKALQEQSIPHDRYVIIIEGSEE
---

---IYDEFSWYKNTTPVTKDLTQLMINKTWYPTICITGAEGIPHTSNAGNVMRTHTTVKV
SVRLPPSFKAADASTVLKEIFETNPPYGAEVTFKMDKCASGWDSPQTRPELEKALGEAPN
LFFGKDHVYMGEGGSIPFMKMLHDRFNCQFIVTGLLGPGSNAHGPNXFLDIPFAKKLLSC
VLLLDAHSKXI
Translated Amino Acid sequence (All Frames) 
Frame A:
ILFSIDKIKLNKMSLNIEKVKEFSNKAWDENILPTLSKYIEIPNQSPLYDAEWATNGYTE
EAIQLLEKWILAQGIKGISCEIKRIEGLTPIIFMVVEATKTEKVKTVLLYGHMDKQPPLT
DAWDEGLHPYKAVIKNNKLYGRGGADDGYSTFASISAIKALQEQSIPHDRYVIIIEGSEE
---

---IYDEFSWYKNTTPVTKDLTQLMINKTWYPTICITGAEGIPHTSNAGNVMRTHTTVKV
SVRLPPSFKAADASTVLKEIFETNPPYGAEVTFKMDKCASGWDSPQTRPELEKALGEAPN
LFFGKDHVYMGEGGSIPFMKMLHDRFNCQFIVTGLLGPGSNAHGPNXFLDIPFAKKLLSC
VLLLDAHSKXI

Frame B:
fyfxlikln*ikch*ilkklknlvikhgmkifyqhfqnilkfqinhhymmqngqpmviqk
kqfny*rngs*hkvskvfhvklkelkd*hqlfsw*skqpklkklklyfymvtwinnhhsl
mhgmkdyihtrqssritnsmvvvvqmmviqhllqslqskhcknnqfhmivm*lslkvpkn
---

---smmnshgtkiplqslki*pn**stklgiqlfaspvlkvfhtlqmlvms*eltpplkl
vfvyhhhskqlmhqly*kkfsklihhmvlklpskwinvlqvgihhkldqn*kkh*eklqi
fslakttytwvkvvpshs*kcymtvsivnlsllvy*vqvqmlmvqxns*ifhlprny*vv
yyy*mlipsxl

Frame C:
fifx**n*ik*nviky*ks*ri***smg*kyftntfkiy*nsksitii*crmgnqwlyrr
snsiiremdlstryqryfm*n*kn*rintnyfhgsrsnqn*ks*nctfiwshg*tttth*
cmg*ritsiqgshqe*qtlwswwcr*wlfnicfnlcnqsiartinst*slcnyh*rfrr-
--

---l**ilmvqkyhssh*rfnpiddqqnlvsnylhhrc*rysthfkcw*chenshhr*s*
csfttiiqss*cincikrnfrn*stiwc*sylqng*mcfrlgfttn*trirksirrssks
flwqrprihg*rwfhpihenvt*pfqlsiyrywfirsrfkcswskxilrysicqeiiklc
iiircsfqvx*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFB774 (AFB774Q) /CSM/AF/AFB7-D/AFB774Q.Seq.d/                       2018   0.0
AFB772 (AFB772Q) /CSM/AF/AFB7-C/AFB772Q.Seq.d/                       1102   0.0
AFL393 (AFL393Q) /CSM/AF/AFL3-D/AFL393Q.Seq.d/                       1096   0.0
AFD881 (AFD881Q) /CSM/AF/AFD8-D/AFD881Q.Seq.d/                       1088   0.0
VFJ593 (VFJ593Q) /CSM/VF/VFJ5-D/VFJ593Q.Seq.d/                       1086   0.0
VFK163 (VFK163Q) /CSM/VF/VFK1-C/VFK163Q.Seq.d/                       1080   0.0
VFB475 (VFB475Q) /CSM/VF/VFB4-D/VFB475Q.Seq.d/                       1080   0.0
AFJ819 (AFJ819Q) /CSM/AF/AFJ8-A/AFJ819Q.Seq.d/                       1080   0.0
AFB653 (AFB653Q) /CSM/AF/AFB6-C/AFB653Q.Seq.d/                       1070   0.0
AFB645 (AFB645Q) /CSM/AF/AFB6-B/AFB645Q.Seq.d/                       1066   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BI435717|BI435717.1 EST538478 P. infestans-challenged potato leaf, compatible reaction Solanum tuberosum cDNA clone PPCCI86 5' sequence, mRNA sequence.    48  0.24  1
BI433279|BI433279.1 EST536040 P. infestans-challenged leaf Solanum tuberosum cDNA clone PPCBC62 5' sequence, mRNA sequence.    48  0.24  1
BQ046631|BQ046631.1 EST595749 P. infestans-challenged potato leaf, incompatible reaction Solanum tuberosum cDNA clone BPLI15C18 5' end, mRNA sequence.    48  0.24  1
BI435853|BI435853.1 EST538614 P. infestans-challenged leaf Solanum tuberosum cDNA clone PPCCL22 5' sequence, mRNA sequence.    48  0.24  1
BG591390|BG591390.1 EST499232 P. infestans-challenged leaf Solanum tuberosum cDNA clone BPLI8D17 5' sequence, mRNA sequence.    48  0.24  1
BQ112173|BQ112173.1 EST597749 mixed potato tissues Solanum tuberosum cDNA clone STMCF59 3' end, mRNA sequence.    48  0.24  1
BI435340|BI435340.1 EST538101 P. infestans-challenged leaf Solanum tuberosum cDNA clone PPCCD08 5' sequence, mRNA sequence.    48  0.24  1
CD821342|CD821342.1 BN25.041I21F011127 BN25 Brassica napus cDNA clone BN25041I21, mRNA sequence.    38  0.37  2
CC535979|CC535979.1 CH240_414D20.TARBAC13P2 CHORI-240 Bos taurus genomic clone CH240_414D20, genomic survey sequence.    36  0.43  2
AC102122|AC102122.3 Mus musculus clone RP23-182N5, WORKING DRAFT SEQUENCE, 10 unordered pieces.    46  0.93  1
dna update 2003. 7.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ384330_1(DQ384330|pid:none) Hartmannella vermiformis succinyl-...   199   1e-49
DQ384331_1(DQ384331|pid:none) Physarum polycephalum succinyl-dia...   191   3e-47
CP001111_978(CP001111|pid:none) Stenotrophomonas maltophilia R55...   187   7e-46
AE008923_2905(AE008923|pid:none) Xanthomonas axonopodis pv. citr...   186   9e-46
AM039952_3097(AM039952|pid:none) Xanthomonas campestris pv. vesi...   186   2e-45
AM743169_1072(AM743169|pid:none) Stenotrophomonas maltophilia K2...   184   5e-45
AM746676_157(AM746676|pid:none) Sorangium cellulosum 'So ce 56' ...   181   4e-44
CP000113_526(CP000113|pid:none) Myxococcus xanthus DK 1622, comp...   179   2e-43
AM502249_240(AM502249|pid:none) Leishmania infantum chromosome 31.    177   7e-43
CT005268_204(CT005268|pid:none) Leishmania major strain Friedlin...   174   4e-42
protein update 2009. 3.27
PSORT 

 psg: 0.70  gvh: 0.29  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for AFB774 is cyt
5' end seq. ID AFB774F
5' end seq. 
>AFB774F.Seq
ATTTTATTTTCNATTGATAAAATTAAATTAAATAAAATGTCATTAAATATTGAAAAAGTT
AAAGAATTTAGTAATAAAGCATGGGATGAAAATATTTTACCAACACTTTCAAAATATATT
GAAATTCCAAATCAATCACCATTATATGATGCAGAATGGGCAACCAATGGTTATACAGAA
GAAGCAATTCAATTATTAGAGAAATGGATCTTAGCACAAGGTATCAAAGGTATTTCATGT
GAAATTAAAAGAATTGAAGGATTAACACCAATTATTTTCATGGTAGTCGAAGCAACCAAA
ACTGAAAAAGTTAAAACTGTACTTTTATATGGTCACATGGATAAACAACCACCACTCACT
GATGCATGGGATGAAGGATTACATCCATACAAGGCAGTCATCAAGAATAACAAACTCTAT
GGTCGTGGTGGTGCAGATGATGGTTATTCAACATTTGCTTCAATCTCTGCAATCAAAGCA
TTGCAAGAACAATCAATTCCACATGATCGTTATGTAATTATCATTGAAGGTTCCGAAGAA
T----------
Length of 5' end seq. 541
3' end seq. ID AFB774Z
3' end seq. 
>AFB774Z.Seq
----------ATCTATGATGAATTCTCATGGTACAAAAATACCACTCCAGTCACTAAAGA
TTTAACCCAATTGATGATCAACAAAACTTGGTATCCAACTATTTGCATCACCGGTGCTGA
AGGTATTCCACACACTTCAAATGCTGGTAATGTCATGAGAACTCACACCACCGTTAAAGT
TAGTGTTCGTTTACCACCATCATTCAAAGCAGCTGATGCATCAACTGTATTAAAAGAAAT
TTTCGAAACTAATCCACCATATGGTGCTGAAGTTACCTTCAAAATGGATAAATGTGCTTC
AGGTTGGGATTCACCACAAACTAGACCAGAATTAGAAAAAGCATTAGGAGAAGCTCCAAA
TCTTTTCTTTGGCAAAGACCACGTATACATGGGTGAAGGTGGTTCCATCCCATTCATGAA
AATGTTACATGACCGTTTCAATTGTCAATTTATCGTTACTGGTTTATTAGGTCCAGGTTC
AAATGCTCATGGTCCAAATNAATTCTTAGATATTCCATTTGCCAAGAAATTATTAAGTTG
TGTATTATTATTAGATGCTCATTCCAAGTTNATTAA
Length of 3' end seq. 566
Connected seq. ID AFB774P
Connected seq. 
>AFB774P.Seq
ATTTTATTTTCNATTGATAAAATTAAATTAAATAAAATGTCATTAAATATTGAAAAAGTT
AAAGAATTTAGTAATAAAGCATGGGATGAAAATATTTTACCAACACTTTCAAAATATATT
GAAATTCCAAATCAATCACCATTATATGATGCAGAATGGGCAACCAATGGTTATACAGAA
GAAGCAATTCAATTATTAGAGAAATGGATCTTAGCACAAGGTATCAAAGGTATTTCATGT
GAAATTAAAAGAATTGAAGGATTAACACCAATTATTTTCATGGTAGTCGAAGCAACCAAA
ACTGAAAAAGTTAAAACTGTACTTTTATATGGTCACATGGATAAACAACCACCACTCACT
GATGCATGGGATGAAGGATTACATCCATACAAGGCAGTCATCAAGAATAACAAACTCTAT
GGTCGTGGTGGTGCAGATGATGGTTATTCAACATTTGCTTCAATCTCTGCAATCAAAGCA
TTGCAAGAACAATCAATTCCACATGATCGTTATGTAATTATCATTGAAGGTTCCGAAGAA
T----------ATCTATGATGAATTCTCATGGTACAAAAATACCACTCCAGTCACTAAAG
ATTTAACCCAATTGATGATCAACAAAACTTGGTATCCAACTATTTGCATCACCGGTGCTG
AAGGTATTCCACACACTTCAAATGCTGGTAATGTCATGAGAACTCACACCACCGTTAAAG
TTAGTGTTCGTTTACCACCATCATTCAAAGCAGCTGATGCATCAACTGTATTAAAAGAAA
TTTTCGAAACTAATCCACCATATGGTGCTGAAGTTACCTTCAAAATGGATAAATGTGCTT
CAGGTTGGGATTCACCACAAACTAGACCAGAATTAGAAAAAGCATTAGGAGAAGCTCCAA
ATCTTTTCTTTGGCAAAGACCACGTATACATGGGTGAAGGTGGTTCCATCCCATTCATGA
AAATGTTACATGACCGTTTCAATTGTCAATTTATCGTTACTGGTTTATTAGGTCCAGGTT
CAAATGCTCATGGTCCAAATNAATTCTTAGATATTCCATTTGCCAAGAAATTATTAAGTT
GTGTATTATTATTAGATGCTCATTCCAAGTTNATTAA
Length of connected seq. 1107
Full length Seq ID -
Full length Seq. -
Length of full length seq. -