>VSB543 (VSB543Q) /CSM/VS/VSB5-B/VSB543Q.Seq.d/
XXXXXXXXXXACAGTATTAGGAAAATATATTTTAGAGGATATCACACCACAACAAGGTGG
ATATGGTAATCAATTATCATTAACAGTAAAAGTAACTGATCCAGAAAAAAGAGAGGTTCT
TAGCAAAACTATGCCATCAAATGGACGTTTTGCTTTTTCAACTCAAGTTGGTGGCGAACA
CAAAATTTGCTTCTCCACCAATACTTCCAAATGGTTTGGTCCATCTGTCAAAACTAGACT
TCATTTAGAAATTGAAGGTGGTGCAGGTGCAAATGATTATGAAGAGATTGCAAAAGTTGA
ACATTTAACTGGTATTGAAATTCATTAAGAAGATTAAATGATAGAGTCAATCAAATTAGA
AAGGAACAAAGTTATCAAAAAGGTCGTGAAATCGTTTTCCGTAATACTAGCGAAAGTACA
AATGCTCGTGTTATGTGGTGGTCAATCATTCAATTAGTTGTTTTAGTTTTAACTGGTGTT
TGGCAAATGAAACATCTTAAATCATTTTTCAAGGCCAAGAAACTTGTATAAACATATTAT
ATTAATAAAATTTAAAAA

---VLGKYILEDITPQQGGYGNQLSLTVKVTDPEKREVLSKTMPSNGRFAFSTQVGGEHK
ICFSTNTSKWFGPSVKTRLHLEIEGGAGANDYEEIAKVEHLTGIEIH*ed*miesikler
nkvikkvvksfsvilakvqmlvlcggqsfn*lf*f*lvfgk*nilnhfsrprnlykhiil
ikfk

Frame A:
---qy*enif*rishhnkvdmvinyh*q*k*liqkkerflaklchqmdvllfqlklvant
kfasppilpnglvhlskldfi*klkvvqvqmimkrlqklni*lvlkfikkik**sqsn*k
gtklskrs*nrfp*y*rkykcscyvvvnhsiscfsfnwclanets*iifqgqetcinily
**nlk


Frame B:
---sirkiyfrgyhtttrwiw*siiinsksn*srkkrgs*qnyaikwtfcffnsswwrtq
nlllhqyfqmvwsicqn*tsfrn*rwcrck*l*rdcks*tfnwy*nslrrlndrvnqirk
eqsyqkgreivfrntsestnarvmwwsiiqlvvlvltgvwqmkhlksffkakklv*tyyi
nki*k


Frame C:
---VLGKYILEDITPQQGGYGNQLSLTVKVTDPEKREVLSKTMPSNGRFAFSTQVGGEHK
ICFSTNTSKWFGPSVKTRLHLEIEGGAGANDYEEIAKVEHLTGIEIH*ed*miesikler
nkvikkvvksfsvilakvqmlvlcggqsfn*lf*f*lvfgk*nilnhfsrprnlykhiil
ikfk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSB543 (VSB543Q) /CSM/VS/VSB5-B/VSB543Q.Seq.d/                       1086   0.0
SSF515 (SSF515Q) /CSM/SS/SSF5-A/SSF515Q.Seq.d/                       1047   0.0
SSC765 (SSC765Q) /CSM/SS/SSC7-C/SSC765Q.Seq.d/                        979   0.0
VSF640 (VSF640Q) /CSM/VS/VSF6-B/VSF640Q.Seq.d/                        870   0.0
SSC770 (SSC770Q) /CSM/SS/SSC7-C/SSC770Q.Seq.d/                         78   2e-13
SSD281 (SSD281Q) /CSM/SS/SSD2-D/SSD281Q.Seq.d/                         40   0.035
VFM273 (VFM273Q) /CSM/VF/VFM2-D/VFM273Q.Seq.d/                         38   0.14
VFL516 (VFL516Q) /CSM/VF/VFL5-A/VFL516Q.Seq.d/                         38   0.14
VFH502 (VFH502Q) /CSM/VF/VFH5-A/VFH502Q.Seq.d/                         38   0.14
VFA738 (VFA738Q) /CSM/VF/VFA7-B/VFA738Q.Seq.d/                         38   0.14

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AL966233|AL966233.1 Xenopus tropicalis EST, clone TGas092m06 5'.    46  1e-04  2
AL896770|AL896770.1 Xenopus tropicalis EST, clone TEgg086m03 5'.    46  1e-04  2
AL958045|AL958045.1 Xenopus tropicalis EST, clone TGas134g16 5'.    46  1e-04  2
AL775119|AL775119.1 Xenopus tropicalis EST, clone TGas076m06 5'.    46  1e-04  2
BQ390980|BQ390980.1 NISC_mq16b11.y1 NICHD XGC Emb5 Silurana tropicalis cDNA clone IMAGE:5308389 5', mRNA sequence.    46  1e-04  2
AL867263|AL867263.1 Xenopus tropicalis EST, clone TEgg099h11 5'.    46  1e-04  2
AL861931|AL861931.1 Xenopus tropicalis EST, clone TEgg114o09 5'.    46  1e-04  2
AL873102|AL873102.1 Xenopus tropicalis EST, clone TEgg124f13 5'.    46  1e-04  2
AL633798|AL633798.1 Xenopus tropicalis EST, clone TGas017b19 5'.    46  1e-04  2
CF221563|CF221563.1 AGENCOURT_15041122 NICHD_XGC_Emb5 Silurana tropicalis cDNA clone IMAGE:6986974 5', mRNA sequence.    46  2e-04  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

EU934418_1(EU934418|pid:none) TSA: Anopheles darlingi AD-295 emp...    94   2e-33
AK294122_1(AK294122|pid:none) Homo sapiens cDNA FLJ53032 complet...   100   2e-33
BC168726_1(BC168726|pid:none) Rattus norvegicus transmembrane em...   100   5e-33
(Q7Z7H5) RecName: Full=Transmembrane emp24 domain-containing pro...   100   5e-33
BT048675_1(BT048675|pid:none) Salmo salar clone ssal-eve-564-043...    96   3e-32
BC123580_1(BC123580|pid:none) Bos taurus transmembrane emp24 pro...    98   7e-32
BT045554_1(BT045554|pid:none) Salmo salar clone ssal-rgf-524-314...    95   7e-32
AK340677_1(AK340677|pid:none) Acyrthosiphon pisum ACYPI010154 mR...    92   9e-32
FN315258_1(FN315258|pid:none) Schistosoma japonicum isolate Anhu...    87   3e-30
EZ000131_1(EZ000131|pid:none) TSA: Schistosoma japonicum SJCHGC0...    87   3e-30

 psg: 0.75  gvh: 0.38  alm: 0.57  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: cytoskeletal
	 12.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VSB543 is cyt

>VSB543Z.Seq
----------ACAGTATTAGGAAAATATATTTTAGAGGATATCACACCACAACAAGGTGG
ATATGGTAATCAATTATCATTAACAGTAAAAGTAACTGATCCAGAAAAAAGAGAGGTTCT
TAGCAAAACTATGCCATCAAATGGACGTTTTGCTTTTTCAACTCAAGTTGGTGGCGAACA
CAAAATTTGCTTCTCCACCAATACTTCCAAATGGTTTGGTCCATCTGTCAAAACTAGACT
TCATTTAGAAATTGAAGGTGGTGCAGGTGCAAATGATTATGAAGAGATTGCAAAAGTTGA
ACATTTAACTGGTATTGAAATTCATTAAGAAGATTAAATGATAGAGTCAATCAAATTAGA
AAGGAACAAAGTTATCAAAAAGGTCGTGAAATCGTTTTCCGTAATACTAGCGAAAGTACA
AATGCTCGTGTTATGTGGTGGTCAATCATTCAATTAGTTGTTTTAGTTTTAACTGGTGTT
TGGCAAATGAAACATCTTAAATCATTTTTCAAGGCCAAGAAACTTGTATAAACATATTAT
ATTAATAAAATTTAAAAA