>VSB521 (VSB521Q) /CSM/VS/VSB5-A/VSB521Q.Seq.d/
XXXXXXXXXXTGCACCAAGCAATGAAACCTTAATGGAAACTGTCAAGAATTTAGAAGCCA
AGAACCACAGAGTTTCAGTCGTTGAAAATGAAGCTGATGCTTTAAATTTATTATTAAATG
AAATCCCAAAAGGTTCTGAAGTCATGTGCGCTAGCAGTTGTACCATTGATGAAATCGGTT
TCAAGAAAATTTATTTCAGTGATGATTCTCCATTCGTCAACGTTCATAAAAAGATTTTAG
CTTTACCAGCTGATAAACAAGCTGATGCTCGTAAAGATGCTTTAACCTCACAATACTTTT
TATCATCTGTAACTGCAATCTCCAAAACTGGAAATGTTTATGTTGCCGACGCTTCAGGTA
CCAGAGTTGGCGGTTTCACTGCTGCTAAAAATTTAATCATCGTTTCTGGTTATAACAAGA
TTGTTGATTCTGATGAATTAGCCGTCCAAAGAACTGAACAATTCTGTTTACCATGTGAAA
GTGTTCGTGCTCGTTGGGCTTACAAAGTTCCAGGTTCAATGATTCAAAATCTTTTGGGTA
TGAAACATGGTGCTCCAAACAAACATCACATTATCCTCATTAAAAAATTATTAGGTTATT
AAATTAATAATTAAAAAAAA

---APSNETLMETVKNLEAKNHRVSVVENEADALNLLLNEIPKGSEVMCASSCTIDEIGF
KKIYFSDDSPFVNVHKKILALPADKQADARKDALTSQYFLSSVTAISKTGNVYVADASGT
RVGGFTAAKNLIIVSGYNKIVDSDELAVQRTEQFCLPCESVRARWAYKVPGSMIQNLLGM
KHGAPNKHHIILIKKLLGY*inn*kk

Frame A:
---APSNETLMETVKNLEAKNHRVSVVENEADALNLLLNEIPKGSEVMCASSCTIDEIGF
KKIYFSDDSPFVNVHKKILALPADKQADARKDALTSQYFLSSVTAISKTGNVYVADASGT
RVGGFTAAKNLIIVSGYNKIVDSDELAVQRTEQFCLPCESVRARWAYKVPGSMIQNLLGM
KHGAPNKHHIILIKKLLGY*inn*kk


Frame B:
---hqamkp*wklsri*kprttefqslkmklml*iyy*mksqkvlkscalavvplmksvs
rkfisvmilhsstfikrf*lyqlinklmlvkml*phntfyhl*lqspklemfmlptlqvp
elavslllki*ssflvitrllilmn*pskelnnsvyhvkvfvlvgltkfqvq*fkifwv*
nmvlqtnitlsslkny*vikliikk


Frame C:
---tkq*nlngncqefrsqepqsfsr*k*s*cfkfiik*npkrf*shvr*qlyh**nrfq
enlfq**fsirqrs*kdfsfts**ts*cs*rcfnltilfiicncnlqnwkclccrrfryq
swrfhcc*kfnhrfwl*qdc*f**isrpkn*tilftm*kcscslglqssrfndsksfgye
twcskqtshyph*kiirlln**lkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSB521 (VSB521Q) /CSM/VS/VSB5-A/VSB521Q.Seq.d/                       1193   0.0
SSK492 (SSK492Q) /CSM/SS/SSK4-D/SSK492Q.Seq.d/                       1193   0.0
SSG784 (SSG784Q) /CSM/SS/SSG7-D/SSG784Q.Seq.d/                       1193   0.0
SSE602 (SSE602Q) /CSM/SS/SSE6-A/SSE602Q.Seq.d/                       1193   0.0
SSI866 (SSI866Q) /CSM/SS/SSI8-C/SSI866Q.Seq.d/                       1185   0.0
SSC791 (SSC791Q) /CSM/SS/SSC7-D/SSC791Q.Seq.d/                       1185   0.0
SSE341 (SSE341Q) /CSM/SS/SSE3-B/SSE341Q.Seq.d/                       1178   0.0
SSK625 (SSK625Q) /CSM/SS/SSK6-B/SSK625Q.Seq.d/                       1172   0.0
VSJ656 (VSJ656Q) /CSM/VS/VSJ6-C/VSJ656Q.Seq.d/                       1170   0.0
VSD182 (VSD182Q) /CSM/VS/VSD1-D/VSD182Q.Seq.d/                       1170   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AJ310674|AJ310674.1 Dictyostelium discoideum 3B gene for prespore-specific protein 3B,.   700  0.0  2
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.   700  0.0  2
AC135415|AC135415.17 Medicago truncatula clone mth2-10b9, WORKING DRAFT SEQUENCE, 3 ordered pieces.    40  0.048  3
BY565063|BY565063.1 Mus musculus activated spleen cDNA, RIKEN full-length enriched library, clone:F830111K10, 3' end partial sequence.    48  0.13  1
L06178|L06178.1 Apis mellifera ligustica complete mitochondrial genome.    32  1.2  4
AC137585|AC137585.2 Mus musculus chromosome UNK clone RP23-355I23, WORKING DRAFT SEQUENCE, 10 unordered pieces.    40  1.6  2
AC073054|AC073054.2 Homo sapiens BAC clone RP11-106F1 from 7, complete sequence.    44  2.0  1
BH691184|BH691184.1 BOMNH72TF BO_2_3_KB Brassica oleracea genomic clone BOMNH72, DNA sequence.    44  2.0  1
BH648455|BH648455.1 BOMIG51TF BO_2_3_KB Brassica oleracea genomic clone BOMIG51, DNA sequence.    44  2.0  1
AC112812|AC112812.3 Rattus norvegicus clone CH230-33G16, *** SEQUENCING IN PROGRESS ***, 4 unordered pieces.    44  2.0  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC115605_5(AC115605|pid:none) Dictyostelium discoideum chromosom...   176   4e-43
AC115605_4(AC115605|pid:none) Dictyostelium discoideum chromosom...   174   1e-42
AY596296_161(AY596296|pid:none) Haloarcula marismortui ATCC 4304...   108   9e-23
AE015927_490(AE015927|pid:none) Clostridium tetani E88, complete...    68   2e-10
AM412317_887(AM412317|pid:none) Clostridium botulinum A str. ATC...    65   2e-09
CP001083_859(CP001083|pid:none) Clostridium botulinum Ba4 str. 6...    65   2e-09
CP001393_1815(CP001393|pid:none) Anaerocellum thermophilum DSM 6...    64   5e-09
BA000016_2602(BA000016|pid:none) Clostridium perfringens str. 13...    58   2e-07
CP000885_3236(CP000885|pid:none) Clostridium phytofermentans ISD...    56   7e-07
CP001393_2285(CP001393|pid:none) Anaerocellum thermophilum DSM 6...    56   9e-07

 psg: 0.75  gvh: 0.32  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 24.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for VSB521 is cyt

>VSB521Z.Seq
----------TGCACCAAGCAATGAAACCTTAATGGAAACTGTCAAGAATTTAGAAGCCA
AGAACCACAGAGTTTCAGTCGTTGAAAATGAAGCTGATGCTTTAAATTTATTATTAAATG
AAATCCCAAAAGGTTCTGAAGTCATGTGCGCTAGCAGTTGTACCATTGATGAAATCGGTT
TCAAGAAAATTTATTTCAGTGATGATTCTCCATTCGTCAACGTTCATAAAAAGATTTTAG
CTTTACCAGCTGATAAACAAGCTGATGCTCGTAAAGATGCTTTAACCTCACAATACTTTT
TATCATCTGTAACTGCAATCTCCAAAACTGGAAATGTTTATGTTGCCGACGCTTCAGGTA
CCAGAGTTGGCGGTTTCACTGCTGCTAAAAATTTAATCATCGTTTCTGGTTATAACAAGA
TTGTTGATTCTGATGAATTAGCCGTCCAAAGAACTGAACAATTCTGTTTACCATGTGAAA
GTGTTCGTGCTCGTTGGGCTTACAAAGTTCCAGGTTCAATGATTCAAAATCTTTTGGGTA
TGAAACATGGTGCTCCAAACAAACATCACATTATCCTCATTAAAAAATTATTAGGTTATT
AAATTAATAATTAAAAAAAA