>VSB185 (VSB185Q) /CSM/VS/VSB1-D/VSB185Q.Seq.d/
ATCCTTTGGGGTTTCTTTTTCCCAATTTGTGCTGCCAAACAAATCATCAATGCAATTCAA
TTTTTCCAAGCTGTTGATGATATCGTTTCTTTAGATGACCAACAAAAATTAAAAAAATAG
ATTAGAAATTAATTATAAATATAAAAATAAAAAAAAAAATAAAAAAAAAAAATTTTT

ILWGFFFPICAAKQIINAIQFFQAVDDIVSLDDQQKLKK*irn*l*i*k*kkk*kkkif

Frame A:
ILWGFFFPICAAKQIINAIQFFQAVDDIVSLDDQQKLKK*irn*l*i*k*kkk*kkkif


Frame B:
sfgvsfsqfvlpnkssmqfnfskllmisfl*mtnkn*knrleinykyknkkknkkkkf


Frame C:
plgflfpnlccqtnhqcnsifpsc**yrffr*ptkikkid*kliinikikkkikkknf

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSB185 (VSB185Q) /CSM/VS/VSB1-D/VSB185Q.Seq.d/                        204   3e-52
SSE755 (SSE755Q) /CSM/SS/SSE7-C/SSE755Q.Seq.d/                        204   3e-52
SSB882 (SSB882Q) /CSM/SS/SSB8-D/SSB882Q.Seq.d/                        204   3e-52
SSI644 (SSI644Q) /CSM/SS/SSI6-B/SSI644Q.Seq.d/                        182   1e-45
SHL407 (SHL407Q) /CSM/SH/SHL4-A/SHL407Q.Seq.d/                         44   7e-04
SSC311 (SSC311Q) /CSM/SS/SSC3-A/SSC311Q.Seq.d/                         34   0.64
VSI361 (VSI361Q) /CSM/VS/VSI3-C/VSI361Q.Seq.d/                         32   2.5
VSG771 (VSG771Q) /CSM/VS/VSG7-C/VSG771Q.Seq.d/                         32   2.5
VSD266 (VSD266Q) /CSM/VS/VSD2-C/VSD266Q.Seq.d/                         32   2.5
VSB454 (VSB454Q) /CSM/VS/VSB4-C/VSB454Q.Seq.d/                         32   2.5

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC118961|AC118961.7 Rattus norvegicus clone CH230-467P14, WORKING DRAFT SEQUENCE.    40   0.83   4
AC145218|AC145218.6 Medicago truncatula clone mth2-34l18, WORKING DRAFT SEQUENCE, 8 unordered pieces.    42   2.3    1
AC126713|AC126713.4 Rattus norvegicus clone CH230-5L14, WORKING DRAFT SEQUENCE, 6 unordered pieces.    42   2.3    1
AC095622|AC095622.6 Rattus norvegicus clone CH230-8G11, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces.    42   2.3    1
AC140779|AC140779.2 Mus musculus clone RP24-383P4, WORKING DRAFT SEQUENCE, 6 unordered pieces.    42   2.3    1
AC135900|AC135900.2 Rattus norvegicus clone CH230-287K17, WORKING DRAFT SEQUENCE, 2 unordered pieces.    42   2.3    1
AG134000|AG134000.1 Pan troglodytes DNA, clone: PTB-146N12.R.      42   2.3    1
CB476363|CB476363.1 jns119_G02.f jns Sus scrofa cDNA 5', mRNA sequence.    42   2.3    1
AC134521|AC134521.23 Medicago truncatula clone mth2-31k24, WORKING DRAFT SEQUENCE, 4 ordered pieces.    32   4.9    3
AC097889|AC097889.6 Rattus norvegicus clone CH230-24I17, *** SEQUENCING IN PROGRESS ***, 1 ordered piece.    40   5.1    5

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3268448
Number of Hits to DB: 218,993,049

 psg: 0.96  gvh: 0.45  alm: 0.40  top: 0.40  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 24.0 %: nuclear
	 12.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for VSB185 is cyt

>VSB185E.Seq
ATCCTTTGGGGTTTCTTTTTCCCAATTTGTGCTGCCAAACAAATCATCAATGCAATTCAA
TTTTTCCAAGCTGTTGATGATATCGTTTCTTTAGATGACCAACAAAAATTAAAAAAATAG
ATTAGAAATTAATTATAAATATAAAAATAAAAAAAAAAATAAAAAAAAAAAATTTTT