NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSB147
Library VS
(Link to library)
Clone ID VSB147
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VSB147E
(Link to Original site)
Representative DNA sequence 
>VSB147 (VSB147Q) /CSM/VS/VSB1-B/VSB147Q.Seq.d/
AATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACT
CAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTC
TGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCA
CACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGT
CAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGC
CCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACT
TTGAGTTTTCAAATATAGTAATCAAATCTAAAAAAAATTTTAAAAAAAAAATAATAAAAA
AAAAAAAATTAAAA
sequence update 2000. 4. 6
Translated Amino Acid sequence 
IYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTADLRSH
TAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*val
*vfkysnqi*kkf*kknnkkkkik
Translated Amino Acid sequence (All Frames) 
Frame A:
nllsrnycprkrywwyllctqss*rycyw*rtsyrfpiilcqssftsirilrfhr*ftfp
hcrssfptmclrslgfnwccqqr*esh*srfshqkekrfsprnprs*qiprktlnnlsrt
lsfqi**snlkkilkkk**kkkn*


Frame B:
IYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTADLRSH
TAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*val
*vfkysnqi*kkf*kknnkkkkik


Frame C:
st**kllpqktllvvftlysivvevlllvknvvsvphyslskliyqy*npsvspliyvpt
lpvklshnvssiiglqlvlstkirkplksl*pperekv*pqksqiltnstknskqfkshf
efsnivikskknfkkkiikkkklk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSB147 (VSB147Q) /CSM/VS/VSB1-B/VSB147Q.Seq.d/                        771   0.0
VHG546 (VHG546Q) /CSM/VH/VHG5-B/VHG546Q.Seq.d/                        771   0.0
VHP749 (VHP749Q) /CSM/VH/VHP7-C/VHP749Q.Seq.d/                        765   0.0
VHO803 (VHO803Q) /CSM/VH/VHO8-A/VHO803Q.Seq.d/                        765   0.0
VHO504 (VHO504Q) /CSM/VH/VHO5-A/VHO504Q.Seq.d/                        765   0.0
VHO104 (VHO104Q) /CSM/VH/VHO1-A/VHO104Q.Seq.d/                        765   0.0
VHI806 (VHI806Q) /CSM/VH/VHI8-A/VHI806Q.Seq.d/                        765   0.0
VHD305 (VHD305Q) /CSM/VH/VHD3-A/VHD305Q.Seq.d/                        765   0.0
VHD106 (VHD106Q) /CSM/VH/VHD1-A/VHD106Q.Seq.d/                        765   0.0
VHB715 (VHB715Q) /CSM/VH/VHB7-A/VHB715Q.Seq.d/                        765   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.   617   0.0    2
BX566526|BX566526.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse82g03_p1c.    84   2e-12  1
BX557682|BX557682.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse33h07_p1c.    84   2e-12  1
BX557472|BX557472.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse32f02_p1c.    84   2e-12  1
BX569353|BX569353.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse99a12_p1c.    84   2e-12  1
BX565591|BX565591.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse78d05_p1c.    84   2e-12  1
BX557368|BX557368.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse31h04_p1c.    84   2e-12  1
BX564658|BX564658.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse73a01_p1c.    84   2e-12  1
BX549986|BX549986.1 Glossina morsitans morsitans (Tseatse fly) EST from adult infected  midgut library, clone Tse110c08_p1c.    84   2e-12  1
AF534908|AF534908.1 Tetrahymena thermophila elongation factor 2 gene, complete cds.    60   3e-12  2
dna update 2004. 2.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q06193) RecName: Full=Elongation factor 2;          Short=EF-2;...   125   4e-28
CP000881_15(CP000881|pid:none) Hemiselmis andersenii chromosome ...   121   9e-27
AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   120   2e-26
AF544973_1(AF544973|pid:none) Ctenopharyngodon idella elongation...   119   3e-26
(P09445) RecName: Full=Elongation factor 2;          Short=EF-2;...   119   3e-26
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   119   4e-26
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   119   4e-26
AK298498_1(AK298498|pid:none) Homo sapiens cDNA FLJ56548 complet...   119   4e-26
AK032997_1(AK032997|pid:none) Mus musculus 12 days embryo male w...   119   4e-26
M19997_1(M19997|pid:none) Human elongation factor 2 (EF-2) mRNA,...   119   4e-26
protein update 2009. 7.28
PSORT 

 psg: 0.75  gvh: 0.48  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VSB147 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID VSB147E
Full length Seq. 
>VSB147E.Seq
AATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACT
CAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTC
TGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCA
CACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGT
CAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGC
CCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACT
TTGAGTTTTCAAATATAGTAATCAAATCTAAAAAAAATTTTAAAAAAAAAATAATAAAAA
AAAAAAAATTAAAA
Length of full length seq. 434