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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VSB127
Library VS
(Link to library)
Clone ID VSB127
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID VSB127E
(Link to Original site)
Representative DNA sequence 
>VSB127 (VSB127Q) /CSM/VS/VSB1-B/VSB127Q.Seq.d/
GTACAACTATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCC
CATCAACCATGAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTG
GTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAAT
ACGACGAATCAGGTCCATCAATTGTCCACAGAAAATGTTTCTAAACAATTAAAACTAGTG
ATGAAAGTGCTTCTCACAAATATTTATGTAAAATATATAATAAAATACATTTAATATTTT
TAGGTTTTGTTTAGTTGTTGATCTTTATCCGACTATTTAAAATCTAAATAATAATTTGTA
sequence update 2000. 4. 6
Translated Amino Acid sequence 
TTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEY
DESGPSIVHRKCF*tiktsdesashkylckiynkihliflgfv*llifirlfki*iiic
Translated Amino Acid sequence (All Frames) 
Frame A:
vqlcsqvlliv*tkn*ll*phqp*klksllhqnvntlsglvdls*lhsqlsnkcgspkkn
ttnqvhqlstenvskqlklvmkvlltniyvkyiikyi*yf*vlfsc*slsdylksk**fv


Frame B:
ynyvpryc*syeqrincfspinhen*nhcstrt*ilcldwwiylsftlnfptnvdlqrri
rrirsincpqkmflnn*n***kcfsqifm*ni**ntfnifrfclvvdlypti*nlnnnl


Frame C:
TTMFPGIADRMNKELTALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKEEY
DESGPSIVHRKCF*tiktsdesashkylckiynkihliflgfv*llifirlfki*iiic
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSB127 (VSB127Q) /CSM/VS/VSB1-B/VSB127Q.Seq.d/                        714   0.0
SLI570 (SLI570Q) /CSM/SL/SLI5-C/SLI570Q.Seq.d/                        714   0.0
SLE773 (SLE773Q) /CSM/SL/SLE7-D/SLE773Q.Seq.d/                        714   0.0
SLA822 (SLA822Q) /CSM/SL/SLA8-A/SLA822Q.Seq.d/                        714   0.0
SLA325 (SLA325Q) /CSM/SL/SLA3-B/SLA325Q.Seq.d/                        714   0.0
SLE147 (SLE147Q) /CSM/SL/SLE1-B/SLE147Q.Seq.d/                        712   0.0
FC-IC0398 (FC-IC0398Q) /CSM/FC-IC/FC-IC0398Q.Seq.d/                   704   0.0
VFH871 (VFH871Q) /CSM/VF/VFH8-C/VFH871Q.Seq.d/                        680   0.0
VFG355 (VFG355Q) /CSM/VF/VFG3-C/VFG355Q.Seq.d/                        680   0.0
VFH614 (VFH614Q) /CSM/VF/VFH6-A/VFH614Q.Seq.d/                        674   0.0
own update 2004. 8. 9
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   714   0.0    9
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   456   e-124  9
K02961|K02961.1 Slime mold (D.discoideum) actin mRNA ITL-1, 3' end.   303   e-121  3
X03281|X03281.1 Dictyostelium discoideum gene for actin A8.       436   e-119  1
K02960|K02960.1 Slime mold (D.discoideum) actin 8 gene, 3' end.   430   e-117  1
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.         398   e-113  3
AX771242|AX771242.1 Sequence 5 from Patent WO03034275.            398   e-112  3
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   398   e-110  2
J01278|J01278.1 Slime mold (D.discoideum) actin mRNA, coding region 3' end.   377   e-108  3
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   398   e-107  8
dna update 2004. 2.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   151   8e-36
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   151   8e-36
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   151   8e-36
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        151   8e-36
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   149   2e-35
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   149   4e-35
(P02578) RecName: Full=Actin-1;  &ATAX(A92886;A03004) &V00002_1(...   149   4e-35
AY582843_1(AY582843|pid:none) Amoebidium parasiticum actin gene,...   149   4e-35
(Q554S6) RecName: Full=Actin-17; AltName: Full=Actin-2-sub 2;  &...   149   4e-35
K02960_1(K02960|pid:none) Dictyostelium discoideum actin 8 gene,...   148   5e-35
protein update 2009. 7.28
PSORT 

 psg: 0.65  gvh: 0.49  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VSB127 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID VSB127E
Full length Seq. 
>VSB127E.Seq
GTACAACTATGTTCCCAGGTATTGCTGATCGTATGAACAAAGAATTAACTGCTTTAGCCC
CATCAACCATGAAAATTAAAATCATTGCTCCACCAGAACGTAAATACTCTGTCTGGATTG
GTGGATCTATCTTAGCTTCACTCTCAACTTTCCAACAAATGTGGATCTCCAAAGAAGAAT
ACGACGAATCAGGTCCATCAATTGTCCACAGAAAATGTTTCTAAACAATTAAAACTAGTG
ATGAAAGTGCTTCTCACAAATATTTATGTAAAATATATAATAAAATACATTTAATATTTT
TAGGTTTTGTTTAGTTGTTGATCTTTATCCGACTATTTAAAATCTAAATAATAATTTGTA
Length of full length seq. 360