>VSA754 (VSA754Q) /CSM/VS/VSA7-C/VSA754Q.Seq.d/
ATTTACCAGCAAGGTTATTTACAAAACAATCACAGGCATTAATTTATAATTATAAAGAAG
CAGCAGTCCAAAGAATGTTAGATTTTGATAATGTTTCACAAAGGGATACACCATCAGTGG
GTGGGTTAATTCATCCAGGATCCGATGGTGGTATGTATAAAGCATTCTTTGGATTTAAAG
AGTTGGTAATACCAGTTTATAATTCAGTATCAGAAGCATGTCAACAATGTCCAAATGCAG
ATGTATTTTTAAATTTTGCATCACATCGTTCAGCTTACCAATCATCGTTATTGGCATTAA
GAGAACCATCGATTCAAACCGTTGTTATCATTGCAGAGGGTGTACCAGAGAATGAAGCAC
GTTCATTGATATCAATTGCAAAGAAATTGGGTAAGGTTATAATCGGACCTGCAACTGTTG
GTGGCATTCAAGCTGGTATCCAATCTGGTGAAATCGAAGTTGTCGAACCAAAATAAACTA
TTTAAACTCAAAATTTTAAATATAAATATAAAAAAAAAAAAAAAAAAAAAAAAA

LPARLFTKQSQALIYNYKEAAVQRMLDFDNVSQRDTPSVGGLIHPGSDGGMYKAFFGFKE
LVIPVYNSVSEACQQCPNADVFLNFASHRSAYQSSLLALREPSIQTVVIIAEGVPENEAR
SLISIAKKLGKVIIGPATVGGIQAGIQSGEIEVVEPK*ti*tqnfkykykkkkkkkk

Frame A:
iyqqgylqnnhrh*fiiikkqqskec*ilimfhkgihhqwvg*fiqdpmvvcikhsldlk
sw*yqfiiqyqkhvnnvqmqmyf*ilhhivqltnhrywh*enhrfkpllslqrvyqrmkh
vh*yqlqrnwvrl*sdlqllvafklvsnlvksklsnqnklfklkilninikkkkkkkk


Frame B:
ftskviyktitginl*l*rssspknvrf**cftkgytisgwvnssrirwwyv*silwi*r
vgntsl*fsirsmstmskcrcifkfcitsfslpiivigikrtidsnrcyhcrgctre*st
fidinckeig*gynrtcncwwhsswypiw*nrscrtkinylnskf*i*i*kkkkkkk


Frame C:
LPARLFTKQSQALIYNYKEAAVQRMLDFDNVSQRDTPSVGGLIHPGSDGGMYKAFFGFKE
LVIPVYNSVSEACQQCPNADVFLNFASHRSAYQSSLLALREPSIQTVVIIAEGVPENEAR
SLISIAKKLGKVIIGPATVGGIQAGIQSGEIEVVEPK*ti*tqnfkykykkkkkkkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSA754 (VSA754Q) /CSM/VS/VSA7-C/VSA754Q.Seq.d/                       1009   0.0
VFK504 (VFK504Q) /CSM/VF/VFK5-A/VFK504Q.Seq.d/                        866   0.0
VHA674 (VHA674Q) /CSM/VH/VHA6-D/VHA674Q.Seq.d/                        646   0.0
VHP546 (VHP546Q) /CSM/VH/VHP5-B/VHP546Q.Seq.d/                        593   e-168
VHA368 (VHA368Q) /CSM/VH/VHA3-C/VHA368Q.Seq.d/                        581   e-165
VHB747 (VHB747Q) /CSM/VH/VHB7-B/VHB747Q.Seq.d/                        539   e-152
VFL190 (VFL190Q) /CSM/VF/VFL1-D/VFL190Q.Seq.d/                        442   e-123
VHC389 (VHC389Q) /CSM/VH/VHC3-D/VHC389Q.Seq.d/                        363   2e-99
VHN334 (VHN334Q) /CSM/VH/VHN3-B/VHN334Q.Seq.d/                        355   4e-97
VHG448 (VHG448Q) /CSM/VH/VHG4-B/VHG448Q.Seq.d/                        353   2e-96

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC079932|AC079932.11 Homo sapiens chromosome 3 clone RP11-527D19, WORKING DRAFT SEQUENCE, 14 unordered pieces.    38   0.003  4
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.           36   0.005  18
BX548042|BX548042.6 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone.    34   0.022  8
AC093289|AC093289.2 Homo sapiens chromosome 5 clone RP11-473L15, complete sequence.    48   0.022  5
BF777513|BF777513.1 NXSI_069_D11_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA clone NXSI_069_D11 5' similar to Arabidopsis thaliana sequence At5g49460 ATP-citrate lyase subunit B see http://mips.gsf.de/proj/thal/db/index.html, mRNA sequence.    50   0.033  1
BQ998939|BQ998939.1 QGG20K18.yg.ab1 QG_EFGHJ lettuce serriola Lactuca sativa cDNA clone QGG20K18, mRNA sequence.    50   0.033  1
AC103297|AC103297.5 Rattus norvegicus clone CH230-21N14, WORKING DRAFT SEQUENCE, 2 unordered pieces.    38   0.038  5
AC140759|AC140759.2 Rattus norvegicus clone CH230-48I18, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    38   0.040  5
AL844506|AL844506.1 Plasmodium falciparum chromosome  7.           40   0.11   14
BZ489912|BZ489912.1 BONOS60TR BO_1.6_2_KB_tot Brassica oleracea genomic clone BONOS60, DNA sequence.    48   0.13   1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54YA0) RecName: Full=Probable ATP-citrate synthase;          E...   286   2e-76
AJ243439_1(AJ243439|pid:none) Cyanophora paradoxa acly gene, exo...   185   5e-46
AJ243483_1(AJ243483|pid:none) Cyanophora paradoxa mRNA for ATP c...   184   2e-45
AP002883_22(AP002883|pid:none) Oryza sativa Japonica Group genom...   167   1e-40
AF290958_1(AF290958|pid:none) Capsicum annuum ATP:citrate lyase ...   167   1e-40
EF571302_1(EF571302|pid:none) Glycyrrhiza uralensis ATP citrate ...   166   5e-40
AC020580_2(AC020580|pid:none) Arabidopsis thaliana chromosome 3 ...   164   1e-39
AB023034_12(AB023034|pid:none) Arabidopsis thaliana genomic DNA,...   164   1e-39
AJ344107_1(AJ344107|pid:none) Lupinus albus mRNA for ATP citrate...   162   4e-39
AF132166_1(AF132166|pid:none) Drosophila melanogaster LD21334 fu...   134   1e-30

 psg: 0.73  gvh: 0.37  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.41  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: mitochondrial
	 24.0 %: cytoplasmic
	 24.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane

>> prediction for VSA754 is mit

>VSA754E.Seq
ATTTACCAGCAAGGTTATTTACAAAACAATCACAGGCATTAATTTATAATTATAAAGAAG
CAGCAGTCCAAAGAATGTTAGATTTTGATAATGTTTCACAAAGGGATACACCATCAGTGG
GTGGGTTAATTCATCCAGGATCCGATGGTGGTATGTATAAAGCATTCTTTGGATTTAAAG
AGTTGGTAATACCAGTTTATAATTCAGTATCAGAAGCATGTCAACAATGTCCAAATGCAG
ATGTATTTTTAAATTTTGCATCACATCGTTCAGCTTACCAATCATCGTTATTGGCATTAA
GAGAACCATCGATTCAAACCGTTGTTATCATTGCAGAGGGTGTACCAGAGAATGAAGCAC
GTTCATTGATATCAATTGCAAAGAAATTGGGTAAGGTTATAATCGGACCTGCAACTGTTG
GTGGCATTCAAGCTGGTATCCAATCTGGTGAAATCGAAGTTGTCGAACCAAAATAAACTA
TTTAAACTCAAAATTTTAAATATAAATATAAAAAAAAAAAAAAAAAAAAAAAAA