>VSA723 (VSA723Q) /CSM/VS/VSA7-A/VSA723Q.Seq.d/
XXXXXXXXXXAAATCCGAATTTAAAGTACCATTCGTTTGTGGTTGTAGAAATCTTGGTGA
AGCTTTACGTCGTATCTCTGAAGGTGCCGCTATGATTCGTACAAAAGGTGAGGCTGGCAC
TGGTGATGTGGTTGAAGCAGTTCGTCATGCTCGTGCAGTTAATAAAGAAATTAAANAAAT
TCAAAATATGGACCCACATGAACTTTACACATACGCCAAGGAAATTCAAGCACCACTCGA
ATTAGTTAAAGAAGTTAAAAGATTAGGTAGACTACCAGTTGTCAATTTCGCTGCTGGTGG
TGTTGCCACTCCCGCTGATGCTGCAATGATGATGCAATTAGGTATGGATGGTGTTTTCGT
TGGTTCTGGTATCTTCAAAAGCGGTGATCCAGCCAAAAGAGCAAAAGCAATCGTTCAAGC
TGTAACTCATTTCAATAATCCTCAAATCGTTGCAAAAGTAAGTGAAAATTTGGGTGAAGC
AATGGTTGGAATTAATGTTGATACTTTAAAAGATAAAGAAAACCAAAATTGGTCAACAAA
AGAAAAATAAATAATTTCTATTTTTATTATTTTTTTTTAAAAAAAAAAAAAAAAAAA

---SEFKVPFVCGCRNLGEALRRISEGAAMIRTKGEAGTGDVVEAVRHARAVNKEIKXIQ
NMDPHELYTYAKEIQAPLELVKEVKRLGRLPVVNFAAGGVATPADAAMMMQLGMDGVFVG
SGIFKSGDPAKRAKAIVQAVTHFNNPQIVAKVSENLGEAMVGINVDTLKDKENQNWSTKE
K*iisifiiff*kkkkk

Frame A:
---npnlkyhsfvvveilvklyvvslkvpl*fvqkvrlalvmwlkqfvmlvqlikklxkf
kiwthmnfthtprkfkhhsn*lkklkd*vdyqlsisllvvlplplmlq**cn*vwmvfsl
vlvsskaviqpkeqkqsfkl*lisiilkslqk*vkiwvkqwlelmlil*kikktkigqqk
knk*flfllfffkkkkkk


Frame B:
---iri*stirlwl*ksw*sftsyl*rcrydsykr*gwhw*cg*sssscscs**rn*xns
kygpt*tlhirqgnssttris*rs*kir*ttscqfrcwwcchsr*ccnddairygwcfrw
fwylqkr*ssqksksnrsscnsfq*ssnrcksk*kfg*sngwn*c*yfkr*rkpklvnkr
kinnfyfyyfflkkkkkk


Frame C:
---SEFKVPFVCGCRNLGEALRRISEGAAMIRTKGEAGTGDVVEAVRHARAVNKEIKXIQ
NMDPHELYTYAKEIQAPLELVKEVKRLGRLPVVNFAAGGVATPADAAMMMQLGMDGVFVG
SGIFKSGDPAKRAKAIVQAVTHFNNPQIVAKVSENLGEAMVGINVDTLKDKENQNWSTKE
K*iisifiiff*kkkkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSA723 (VSA723Q) /CSM/VS/VSA7-A/VSA723Q.Seq.d/                       1102   0.0
VSH148 (VSH148Q) /CSM/VS/VSH1-B/VSH148Q.Seq.d/                       1074   0.0
VFI277 (VFI277Q) /CSM/VF/VFI2-D/VFI277Q.Seq.d/                       1037   0.0
VFH235 (VFH235Q) /CSM/VF/VFH2-B/VFH235Q.Seq.d/                        948   0.0
VSH175 (VSH175Q) /CSM/VS/VSH1-D/VSH175Q.Seq.d/                        946   0.0
VSE187 (VSE187Q) /CSM/VS/VSE1-D/VSE187Q.Seq.d/                        920   0.0
VFB322 (VFB322Q) /CSM/VF/VFB3-A/VFB322Q.Seq.d/                        904   0.0
SSH452 (SSH452Q) /CSM/SS/SSH4-C/SSH452Q.Seq.d/                        854   0.0
VFH206 (VFH206Q) /CSM/VF/VFH2-A/VFH206Q.Seq.d/                        819   0.0
VFF330 (VFF330Q) /CSM/VF/VFF3-B/VFF330Q.Seq.d/                        807   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

Z99164|Z99164.2 S.pombe chromosome I cosmid c29B12.               72  5e-16  3
AC140547|AC140547.8 Medicago truncatula clone mth2-17p16, WORKING DRAFT SEQUENCE, 6 ordered pieces.    68  5e-16  6
AX569778|AX569778.1 Sequence 2987 from Patent WO02077021.         64  1e-15  3
AL409181|AL409181.1 T3 end of clone AV0AA013C07 of library AV0AA from strain CBS 379 of Saccharomyces exiguus.    58  1e-15  3
AR071395|AR071395.1 Sequence 4 from patent US 5910580.            64  5e-15  3
BD003705|BD003705.1 Polynucleotide of Streptococcus pneumoniae and sequence.    64  1e-14  3
AR218793|AR218793.1 Sequence 25 from patent US 6420135.           64  1e-14  3
AE008502|AE008502.1 Streptococcus pneumoniae R6 section 118 of 184 of the complete genome.    64  1e-13  3
AE007442|AE007442.1 Streptococcus pneumoniae TIGR4  section 125 of 194 of the complete genome.    64  1e-13  3
BJ167538|BJ167538.1 Physcomitrella patens subsp. patens cDNA clone:pph15h06, 3' end, single read.    52  7e-13  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54J47) RecName: Full=Probable pyridoxine biosynthesis protein ...   320   2e-86
(A9WFT9) RecName: Full=Pyridoxal biosynthesis lyase pdxS;       ...   234   2e-60
CP001337_2843(CP001337|pid:none) Chloroflexus aggregans DSM 9485...   232   7e-60
CP000875_3792(CP000875|pid:none) Herpetosiphon aurantiacus ATCC ...   231   9e-60
CP001324_242(CP001324|pid:none) Micromonas sp. RCC299 chromosome...   231   1e-59
(A5UY94) RecName: Full=Pyridoxal biosynthesis lyase pdxS;       ...   230   2e-59
AP008226_704(AP008226|pid:none) Thermus thermophilus HB8 genomic...   226   3e-58
(Q5SKD9) RecName: Full=Pyridoxal biosynthesis lyase pdxS;       ...   226   3e-58
CP000583_18(CP000583|pid:none) Ostreococcus lucimarinus CCE9901 ...   225   6e-58
CP000232_9(CP000232|pid:none) Moorella thermoacetica ATCC 39073,...   225   6e-58

 psg: 0.77  gvh: 0.40  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.16  mip: 0.03
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 16.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VSA723 is cyt

>VSA723Z.Seq
----------AAATCCGAATTTAAAGTACCATTCGTTTGTGGTTGTAGAAATCTTGGTGA
AGCTTTACGTCGTATCTCTGAAGGTGCCGCTATGATTCGTACAAAAGGTGAGGCTGGCAC
TGGTGATGTGGTTGAAGCAGTTCGTCATGCTCGTGCAGTTAATAAAGAAATTAAANAAAT
TCAAAATATGGACCCACATGAACTTTACACATACGCCAAGGAAATTCAAGCACCACTCGA
ATTAGTTAAAGAAGTTAAAAGATTAGGTAGACTACCAGTTGTCAATTTCGCTGCTGGTGG
TGTTGCCACTCCCGCTGATGCTGCAATGATGATGCAATTAGGTATGGATGGTGTTTTCGT
TGGTTCTGGTATCTTCAAAAGCGGTGATCCAGCCAAAAGAGCAAAAGCAATCGTTCAAGC
TGTAACTCATTTCAATAATCCTCAAATCGTTGCAAAAGTAAGTGAAAATTTGGGTGAAGC
AATGGTTGGAATTAATGTTGATACTTTAAAAGATAAAGAAAACCAAAATTGGTCAACAAA
AGAAAAATAAATAATTTCTATTTTTATTATTTTTTTTTAAAAAAAAAAAAAAAAAAA