>VSA646 (VSA646Q) /CSM/VS/VSA6-B/VSA646Q.Seq.d/
XXXXXXXXXXAAATGTCTTCAGGTATTGCTTTAGCTCCAAACTGCGTTTCAACCTTCAAC
GATTTAAAGTTAGGTCGTAAATATGGTGGTATCATCTACAGAATCTCTGACGATTCAAAA
GAAATCATTGTTGACTCCACCTTACCAGCTGGTTGTTCATTCGATGAATTCACCAAATGT
CTCCCAGAAAACGAATGTAGATACGTTGTCCTCGACTATCAATACAAAGAAGAAGGTGCC
CAAAAGAGCAAAATCTGTTTTGTTGCCTGGTGCCCAGATACCGCCAACATTAAAAAGAAA
ATGATGGCCACCTCATCAAAAGACTCTTTACGTAAAGCCTGTGTTGGTATCCAAGTTGAA
ATCCAAGGTACCGATGCCTCTGAAGTCAAAGATTCATGTTTCTATGAAAAATGTACCAAA
ATCTAAATAATTTGAAAAAATTTAAAAAAAAAAAAAAAAAAAAAACTCCCGAGGAACGAA
TTCAATATTTATAAATAGTAATAAATCTCCTCATACATATAATTTAATAAAATAAAAAAA
AAAAAAAAAA

---MSSGIALAPNCVSTFNDLKLGRKYGGIIYRISDDSKEIIVDSTLPAGCSFDEFTKCL
PENECRYVVLDYQYKEEGAQKSKICFVAWCPDTANIKKKMMATSSKDSLRKACVGIQVEI
QGTDASEVKDSCFYEKCTKI*ii*knlkkkkkkklprnefniyk***issyi*fnkikkk
kk

Frame A:
---nvfrycfssklrfnlqrfkvrs*iwwyhlqnl*rfkrnhc*lhltswlfir*ihqms
prkrm*ircprlsiqrrrcpkeqnlfcclvpryrqh*kendghlikrlft*slcwyps*n
pryrcl*sqrfmfl*kmyqnlnnlkkfkkkkkkktpeeriqyl*ivinllihii**nkkk
kk


Frame B:
---MSSGIALAPNCVSTFNDLKLGRKYGGIIYRISDDSKEIIVDSTLPAGCSFDEFTKCL
PENECRYVVLDYQYKEEGAQKSKICFVAWCPDTANIKKKMMATSSKDSLRKACVGIQVEI
QGTDASEVKDSCFYEKCTKI*ii*knlkkkkkkklprnefniyk***issyi*fnkikkk
kk


Frame C:
---clqvll*lqtafqpsti*s*vvnmvvsstesltiqkkslltppyqlvvhsmnspnvs
qktnvdtlsstintkkkvpkraksvllpgaqipptlkrk*wpphqktlyvkpvlvsklks
kvpmplkskihvsmknvpksk*feki*kkkkkknsrgtnsifinsnksphtynlik*kkk
kk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSJ890 (VSJ890Q) /CSM/VS/VSJ8-D/VSJ890Q.Seq.d/                        781   0.0
VSJ584 (VSJ584Q) /CSM/VS/VSJ5-D/VSJ584Q.Seq.d/                        781   0.0
VSJ550 (VSJ550Q) /CSM/VS/VSJ5-C/VSJ550Q.Seq.d/                        781   0.0
VSJ437 (VSJ437Q) /CSM/VS/VSJ4-B/VSJ437Q.Seq.d/                        781   0.0
VSJ243 (VSJ243Q) /CSM/VS/VSJ2-B/VSJ243Q.Seq.d/                        781   0.0
VSI135 (VSI135Q) /CSM/VS/VSI1-B/VSI135Q.Seq.d/                        781   0.0
VSG665 (VSG665Q) /CSM/VS/VSG6-C/VSG665Q.Seq.d/                        781   0.0
VSE444 (VSE444Q) /CSM/VS/VSE4-B/VSE444Q.Seq.d/                        781   0.0
VSD383 (VSD383Q) /CSM/VS/VSD3-D/VSD383Q.Seq.d/                        781   0.0
VSD320 (VSD320Q) /CSM/VS/VSD3-A/VSD320Q.Seq.d/                        781   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

D37981|D37981.1 Dictyostelium discoidium DDCOF2 gene for cofilin, complete cds.   781  0.0  2
D37980|D37980.1 Dictyostelium discoidium DDCOF1 gene for cofilin, complete cds (exon1-2).   773  0.0  2
X04004|X04004.1 Dictyostelium discoideum mRNA for contact site A (csA) protein.   168  5e-38  1
CC234827|CC234827.1 CH261-7N19_RM1.2 CH261 Gallus gallus genomic clone CH261-7N19, DNA sequence.    46  0.45  1
AC135132|AC135132.2 Rattus norvegicus clone CH230-240G18, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    44  1.3  2
AC096355|AC096355.12 Rattus norvegicus clone CH230-34N3, WORKING DRAFT SEQUENCE, 3 unordered pieces.    44  1.3  2
L46781|L46781.1 Rattus norvegicus plasma membrane calcium ATPase isoform 3 gene, exon 12.    44  1.8  1
BQ108127|BQ108127.1 shrpID000306 LvG Litopenaeus vannamei cDNA, mRNA sequence.    44  1.8  1
CB784341|CB784341.1 AMGNNUC:NRHY4-00139-G3-A W Rat hypothalamus (10464) Rattus norvegicus cDNA clone nrhy4-00139-g3 5', mRNA sequence.    44  1.8  1
CB727158|CB727158.1 AMGNNUC:NRHY7-00026-B3-A nrhy7 (10850) Rattus norvegicus cDNA clone nrhy7-00026-b3 5', mRNA sequence.    44  1.8  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54706) RecName: Full=Cofilin-1;  &D37980_1(D37980|pid:none) &D...   280   1e-74
(P37167) RecName: Full=Actophorin;  &M93361_1(M93361|pid:none)        130   2e-29
FM992692_38(FM992692|pid:none) Candida dubliniensis CD36 chromos...   125   7e-28
(Q96VU9) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...   125   1e-27
AY428595_1(AY428595|pid:none) Helianthus annuus clone HA10-7 put...   123   3e-27
CP000501_299(CP000501|pid:none) Pichia stipitis CBS 6054 chromos...   120   3e-26
(Q9HF97) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...   119   7e-26
(Q6CQ22) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...   119   7e-26
CU928179_306(CU928179|pid:none) Zygosaccharomyces rouxii strain ...   116   3e-25
(Q03048) RecName: Full=Cofilin; AltName: Full=Actin-depolymerizi...   116   3e-25

 psg: 0.91  gvh: 0.46  alm: 0.50  top: 0.67  tms: 0.00  mit: 0.27  mip: 0.06
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: peroxisomal
	  4.0 %: endoplasmic reticulum

>> prediction for VSA646 is nuc

>VSA646Z.Seq
----------AAATGTCTTCAGGTATTGCTTTAGCTCCAAACTGCGTTTCAACCTTCAAC
GATTTAAAGTTAGGTCGTAAATATGGTGGTATCATCTACAGAATCTCTGACGATTCAAAA
GAAATCATTGTTGACTCCACCTTACCAGCTGGTTGTTCATTCGATGAATTCACCAAATGT
CTCCCAGAAAACGAATGTAGATACGTTGTCCTCGACTATCAATACAAAGAAGAAGGTGCC
CAAAAGAGCAAAATCTGTTTTGTTGCCTGGTGCCCAGATACCGCCAACATTAAAAAGAAA
ATGATGGCCACCTCATCAAAAGACTCTTTACGTAAAGCCTGTGTTGGTATCCAAGTTGAA
ATCCAAGGTACCGATGCCTCTGAAGTCAAAGATTCATGTTTCTATGAAAAATGTACCAAA
ATCTAAATAATTTGAAAAAATTTAAAAAAAAAAAAAAAAAAAAAACTCCCGAGGAACGAA
TTCAATATTTATAAATAGTAATAAATCTCCTCATACATATAATTTAATAAAATAAAAAAA
AAAAAAAAAA