>VSA531 (VSA531Q) /CSM/VS/VSA5-B/VSA531Q.Seq.d/
ATTTGCTTCATTTTTTTCTTCTACATCCACATCAGCAACTTTAAATGGTTCATCAAATAA
TAAAACAGTAGTTCCAAAAAGTTTAGAAAATATTTCAAGTGCATCCCAATTCAATTTTAG
CACTTTCTTTATCATTTCAGATGATGACGATGTTTGATAATAATAATATAGGATCATAAA
AATCAAAAAAAAAAAAAAAGAAATAAACAAAGAAGAAGAAAACAAGTAAGAAAATGGAAA
ACAAAAAAAAAAATAAAAAAAAAAAAAATAAAAAAAATATATTTAAACAATTAAAAA

FASFFSSTSTSATLNGSSNNKTVVPKSLENISSASQFNFSTFFIISDDDDV****yriik
ikkkkkeinkeeenk*engkqkkk*kkkk*kkyi*tik

Frame A:
icfifffyihisnfkwfik**nssskkfrkyfkcipiqf*hflyhfr**rcliiii*dhk
nqkkkkrnkqrrrkqvrkwktkkkikkkkikkiylnn*k


Frame B:
FASFFSSTSTSATLNGSSNNKTVVPKSLENISSASQFNFSTFFIISDDDDV****yriik
ikkkkkeinkeeenk*engkqkkk*kkkk*kkyi*tik


Frame C:
llhffllhphqql*mvhqiikq*fqkv*kifqvhpnsilalslsfqmmtmfdnnnigs*k
skkkkkk*tkkkktskkmenkkknkkkknkknifkqlk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSA531 (VSA531Q) /CSM/VS/VSA5-B/VSA531Q.Seq.d/                        331   3e-90
VFG622 (VFG622Q) /CSM/VF/VFG6-A/VFG622Q.Seq.d/                         42   0.005
CHE463 (CHE463Q) /CSM/CH/CHE4-C/CHE463Q.Seq.d/                         42   0.005
CFH708 (CFH708Q) /CSM/CF/CFH7-A/CFH708Q.Seq.d/                         42   0.005
AHM467 (AHM467Q) /CSM/AH/AHM4-C/AHM467Q.Seq.d/                         42   0.005
SHE852 (SHE852Q) /CSM/SH/SHE8-C/SHE852Q.Seq.d/                         38   0.072
VSI742 (VSI742Q) /CSM/VS/VSI7-B/VSI742Q.Seq.d/                         36   0.28
VSH604 (VSH604Q) /CSM/VS/VSH6-A/VSH604Q.Seq.d/                         36   0.28
VSF726 (VSF726Q) /CSM/VS/VSF7-B/VSF726Q.Seq.d/                         36   0.28
VSF720 (VSF720Q) /CSM/VS/VSF7-A/VSF720Q.Seq.d/                         36   0.28

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC115598|AC115598.2 Dictyostelium discoideum chromosome 2 map 581427-735498 strain AX4, complete sequence.    32   2e-06  10
AC117176|AC117176.2 Dictyostelium discoideum chromosome 2 map 5018074-5200947 strain AX4, complete sequence.    34   5e-06  10
AC006281|AC006281.8 Plasmodium falciparum chromosome 12 clone 3D7, *** SEQUENCING IN PROGRESS ***, 2 unordered pieces.    34   5e-05  8
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.           38   2e-04  11
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    32   5e-04  11
AE014840|AE014840.1 Plasmodium falciparum 3D7 chromosome 11 section 5 of 8 of the complete sequence.    36   0.001  9
AL035476|AL035476.7 Plasmodium falciparum MAL4P3.                  34   0.001  12
AC106740|AC106740.3 Homo sapiens chromosome 5 clone RP11-100E22, complete sequence.    38   0.001  5
AL844505|AL844505.1 Plasmodium falciparum chromosome  6.           36   0.002  12
AL844507|AL844507.1 Plasmodium falciparum chromosome 8.            34   0.003  15

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3268448
Number of Hits to DB: 164,325,059

 psg: 0.92  gvh: 0.48  alm: 0.50  top: 0.53  tms: 0.00  mit: 0.38  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: mitochondrial
	 28.0 %: nuclear
	 24.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: endoplasmic reticulum

>> prediction for VSA531 is mit

>VSA531E.Seq
ATTTGCTTCATTTTTTTCTTCTACATCCACATCAGCAACTTTAAATGGTTCATCAAATAA
TAAAACAGTAGTTCCAAAAAGTTTAGAAAATATTTCAAGTGCATCCCAATTCAATTTTAG
CACTTTCTTTATCATTTCAGATGATGACGATGTTTGATAATAATAATATAGGATCATAAA
AATCAAAAAAAAAAAAAAAGAAATAAACAAAGAAGAAGAAAACAAGTAAGAAAATGGAAA
ACAAAAAAAAAAATAAAAAAAAAAAAAATAAAAAAAATATATTTAAACAATTAAAAA