>VSA527 (VSA527Q) /CSM/VS/VSA5-B/VSA527Q.Seq.d/
XXXXXXXXXXAACAACAGGTATGATGATGATGATGGAATGGATATGGGACCCATTGATCA
TGATTTAAGTCCTGCTATTAACTATGGGGATTCAAGAAATTTTCAACAACCACAACCAAT
ACAACAACAACAACAACAACAAANGCAACCACATTACAATAATCAATCAATGCCACCATC
ATCATTAGGTTCAACTTCAAGTTATTTAACAACCAATAGTTAAAAACCTGTTATCAGTTA
TCGTGGTATAAACTTGGAGGCTGATGACGATGATGATGATGAAGAAAATGGTTTTAATCA
AAATGAAAATGAAGAACCAAATATTCAAAATAGTCAAAATCAATAAACANGTATAATAAT
AATAATAATAAAATTATTTTAAAATTAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAA

---NRYDDDDGMDMGPIDHDLSPAINYGDSRNFQQPQPIQQQQQQQXQPHYNNQSMPPSS
LGSTSSYLTTNS*kpvisyrginleaddddddeengfnqneneepniqnsqnq*txiiii
iiklf*n*lkkkkkkkkkkkkkkkkkkkkkkkkkkkkkk

Frame A:
---ttgmmmmmewiwdplimi*vllltmgiqeifnnhnqynnnnnnkxnhitiinqchhh
h*vqlqvi*qpivknllsvivv*twrlmtmmmmkkmvlikmkmknqifkivkinkxv***
***nyfkin*kkkkkkkkkkkkkkkkkkkkkkkkkkkkkk


Frame B:
---qqv****wngygth*s*fkscy*lwgfkkfsttttntttttttxattlq*sinatii
irfnfklfnnq*lktcyqlswyklgg**r****rkwf*sk*k*rtkysk*sksinxynnn
nnkiilklikkkkkkkkkkkkkkkkkkkkkkkkkkkkkkk


Frame C:
---NRYDDDDGMDMGPIDHDLSPAINYGDSRNFQQPQPIQQQQQQQXQPHYNNQSMPPSS
LGSTSSYLTTNS*kpvisyrginleaddddddeengfnqneneepniqnsqnq*txiiii
iiklf*n*lkkkkkkkkkkkkkkkkkkkkkkkkkkkkkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSA527 (VSA527Q) /CSM/VS/VSA5-B/VSA527Q.Seq.d/                        396   e-109
VHN373 (VHN373Q) /CSM/VH/VHN3-D/VHN373Q.Seq.d/                         54   2e-06
VHD796 (VHD796Q) /CSM/VH/VHD7-D/VHD796Q.Seq.d/                         54   2e-06
SFJ638 (SFJ638Q) /CSM/SF/SFJ6-B/SFJ638Q.Seq.d/                         46   5e-04
SFJ538 (SFJ538Q) /CSM/SF/SFJ5-B/SFJ538Q.Seq.d/                         46   5e-04
AHO874 (AHO874Q) /CSM/AH/AHO8-D/AHO874Q.Seq.d/                         44   0.002
VHJ822 (VHJ822Q) /CSM/VH/VHJ8-A/VHJ822Q.Seq.d/                         44   0.002
VHC547 (VHC547Q) /CSM/VH/VHC5-B/VHC547Q.Seq.d/                         44   0.002
SHL125 (SHL125Q) /CSM/SH/SHL1-B/SHL125Q.Seq.d/                         44   0.002

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   373  e-136  4
U41289|U41289.1 Dictyostelium discoideum K7 kinesin-like protein mRNA, complete cds.    46  9e-06  4
AF462563|AF462563.1 Hemibagrus nemurus clone PCTB3-1 microsatellite sequence.    44  5e-05  2
BF610997|BF610997.1 dd96d11.y1 Wellcome CRC pRN3 oocyte Xenopus laevis cDNA clone IMAGE:3436460 5' similar to SW:NPMN_XENLA O42584 NUCLEOPLASMIN-LIKE PROTEIN NOVA. ;, mRNA sequence.    50  7e-05  2
AW765776|AW765776.1 da77a12.y1 Harland stage 19-23 Xenopus laevis cDNA clone IMAGE:3200926 5' similar to TR:O42584 O42584 NOVA. ;, mRNA sequence.    50  7e-05  2
BU011960|BU011960.1 QGJ17K10.yg.ab1 QG_EFGHJ lettuce serriola Lactuca sativa cDNA clone QGJ17K10, mRNA sequence.    50  8e-05  2
AA035437|AA035437.1 zk25f12.r1 Soares_pregnant_uterus_NbHPU Homo sapiens cDNA clone IMAGE:471599 5' similar to gb:X51602_cds1 VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR 1 (HUMAN);contains element OFR repetitive element ;, mRNA sequence.    46  9e-05  2
BQ988536|BQ988536.1 QGF15D01.yg.ab1 QG_EFGHJ lettuce serriola Lactuca sativa cDNA clone QGF15D01, mRNA sequence.    50  9e-05  2
AF520891|AF520891.1 Aphelocoma coerulescens clone ApCo31 microsatellite sequence.    46  1e-04  2
CD329868|CD329868.1 AGENCOURT_14291777 NICHD_XGC_Brn1 Xenopus laevis cDNA clone IMAGE:6950380 5', mRNA sequence.    50  1e-04  2

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3268448
Number of Hits to DB: 261,529,421

 psg: 0.75  gvh: 0.25  alm: 0.65  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: nuclear
	 36.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	 12.0 %: mitochondrial

>> prediction for VSA527 is nuc

>VSA527Z.Seq
----------AACAACAGGTATGATGATGATGATGGAATGGATATGGGACCCATTGATCA
TGATTTAAGTCCTGCTATTAACTATGGGGATTCAAGAAATTTTCAACAACCACAACCAAT
ACAACAACAACAACAACAACAAANGCAACCACATTACAATAATCAATCAATGCCACCATC
ATCATTAGGTTCAACTTCAAGTTATTTAACAACCAATAGTTAAAAACCTGTTATCAGTTA
TCGTGGTATAAACTTGGAGGCTGATGACGATGATGATGATGAAGAAAATGGTTTTAATCA
AAATGAAAATGAAGAACCAAATATTCAAAATAGTCAAAATCAATAAACANGTATAATAAT
AATAATAATAAAATTATTTTAAAATTAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAA