>VSA514 (VSA514Q) /CSM/VS/VSA5-A/VSA514Q.Seq.d/
CAATGAAAGCATTAAAGTATATGTTACTTTGTAAAATTATGACCAACCAAACTGATGATG
TTCATGCATTAGTTAATGGTAAAATTGGTTTGAAATATCATAACAATAGATCCATTGAAG
CAATTACTCAAATTTCAAAATCTCATGCAAAGAGATCACTTCATATGTTCCAAGAGGTAA
CCAAAGAATTTTCTGATCAATTATCTGATGATCCAATCATTCATAGTCATTTAACTGAAT
TATATAGTAATCTTCTTGAACAAGATTTATGTCGTATTATTGAACCATTCTCAAGGGTTG
AAATTTCACATATTGCAAAATTAATTGATTTACCAGTTGATGTTGTTGAAAGAAAATTAT
CACTTATGATCCTTGATAAGAAATATAATGGTATCCTCGATCAAGGTACTGGTACTTTAA
TTGTATTTGAAGAACAAAAAGAAGATAAATTATTTAATTGTTCTTTAGAAACTATTGGTG
CTTTAAGTCGTGTTGTTGATTTACTTTATGAAAAAACAAACAAACTTTCTTAAATTATTT
CTTTGGTCCATCTCCCTCAATTACCAAAGATAAAAAAAAAAAAAAAAAAATATAAATAAA
AATAGAAAAACAAAC

MKALKYMLLCKIMTNQTDDVHALVNGKIGLKYHNNRSIEAITQISKSHAKRSLHMFQEVT
KEFSDQLSDDPIIHSHLTELYSNLLEQDLCRIIEPFSRVEISHIAKLIDLPVDVVERKLS
LMILDKKYNGILDQGTGTLIVFEEQKEDKLFNCSLETIGALSRVVDLLYEKTNKLS*iis
lvhlpqlpkikkkkkkyk*k*knk

Frame A:
q*kh*sicyfvkl*ptklmmfmh*lmvklv*niitidplkqllkfqnlmqrdhficskr*
pknflinylmiqsfivi*lnyiviflnkiyvvllnhsqglkfhilqn*liyqlmllkeny
hl*slirnimvssikvlvl*lylknkkkinylivl*kllvl*vvlliyfmkkqtnflklf
lwsislnyqr*kkkkkninknrktn


Frame B:
nesikvyvtl*nydqpn**cscis*w*nwfeis*q*ih*snysnfkisckeitsyvprgn
qrif*sii**snhs*sfn*ii**ss*trfmsyy*tilkg*nftyckin*fts*cc*kkii
tydp**ei*wyprsrywyfnci*rtkrr*ii*lffrnywcfkscc*ftl*knkqtflnyf
fgpspsitkdkkkkkki*ikiekq


Frame C:
MKALKYMLLCKIMTNQTDDVHALVNGKIGLKYHNNRSIEAITQISKSHAKRSLHMFQEVT
KEFSDQLSDDPIIHSHLTELYSNLLEQDLCRIIEPFSRVEISHIAKLIDLPVDVVERKLS
LMILDKKYNGILDQGTGTLIVFEEQKEDKLFNCSLETIGALSRVVDLLYEKTNKLS*iis
lvhlpqlpkikkkkkkyk*k*knk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSA514 (VSA514Q) /CSM/VS/VSA5-A/VSA514Q.Seq.d/                       1132   0.0
SLG702 (SLG702Q) /CSM/SL/SLG7-A/SLG702Q.Seq.d/                       1124   0.0
VFE589 (VFE589Q) /CSM/VF/VFE5-D/VFE589Q.Seq.d/                       1090   0.0
VSD281 (VSD281Q) /CSM/VS/VSD2-D/VSD281Q.Seq.d/                       1084   0.0
SFB566 (SFB566Q) /CSM/SF/SFB5-C/SFB566Q.Seq.d/                       1084   0.0
AFC122 (AFC122Q) /CSM/AF/AFC1-A/AFC122Q.Seq.d/                       1070   0.0
AFM831 (AFM831Q) /CSM/AF/AFM8-B/AFM831Q.Seq.d/                       1063   0.0
AFO868 (AFO868Q) /CSM/AF/AFO8-C/AFO868Q.Seq.d/                       1053   0.0
AFG628 (AFG628Q) /CSM/AF/AFG6-B/AFG628Q.Seq.d/                       1047   0.0
AFO642 (AFO642Q) /CSM/AF/AFO6-B/AFO642Q.Seq.d/                       1043   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.    34  0.028  11
AC141749|AC141749.1 Apis mellifera clone CH224-59E23, WORKING DRAFT SEQUENCE, 14 unordered pieces.    38  0.032  5
X56203|X56203.1 P.falciparum LSA-1 gene for liver stage antigen.    40  0.034  3
BU570859|BU570859.1 AGENCOURT_10403632 NIH_MGC_82 Homo sapiens cDNA clone IMAGE:6622774 5', mRNA sequence.    44  0.041  2
AC068174|AC068174.2 Homo sapiens chromosome X clone RP11-703D12 map X, WORKING DRAFT SEQUENCE, 39 unordered pieces.    32  0.076  8
CC859331|CC859331.1 NDL.125M5.T7 Notre Dame Liverpool Aedes aegypti genomic clone NotreDame Liverpool-125M5, genomic survey sequence.    34  0.096  3
BQ412650|BQ412650.1 GA__Ed0060G10r Gossypium arboreum 7-10 dpa fiber library Gossypium arboreum cDNA clone GA__Ed0060G10r, mRNA sequence.    44  0.11  2
AC108425|AC108425.4 Mus musculus clone RP23-119I1, WORKING DRAFT SEQUENCE, 8 unordered pieces.    40  0.12  4
AC125272|AC125272.2 Mus musculus clone RP24-155E2, LOW-PASS SEQUENCE SAMPLING.    48  0.13  1
AC006572|AC006572.2 Homo sapiens, clone hRPK.60_A_1, complete sequence.    36  0.14  8

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54UB5) RecName: Full=26S proteasome non-ATPase regulatory subu...   345   8e-94
AM424416_1(AM424416|pid:none) Vitis vinifera contig VV78X196128....   197   3e-49
AY230837_1(AY230837|pid:none) Arabidopsis thaliana 26S proteasom...   195   1e-48
AY230834_1(AY230834|pid:none) Arabidopsis thaliana 26S proteasom...   195   1e-48
A86414(A86414) hypothetical protein F28N24.15 - Arabidopsis thal...   195   1e-48
EF146969_1(EF146969|pid:none) Populus trichocarpa clone WS01225_...   194   1e-48
BT053774_1(BT053774|pid:none) Zea mays full-length cDNA clone ZM...   193   3e-48
EU969379_1(EU969379|pid:none) Zea mays clone 329471 26S proteaso...   192   5e-48
AK064788_1(AK064788|pid:none) Oryza sativa Japonica Group cDNA c...   192   5e-48
BT069707_1(BT069707|pid:none) Zea mays full-length cDNA clone ZM...   192   6e-48

 psg: 0.56  gvh: 0.45  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 20.0 %: nuclear
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VSA514 is cyt

>VSA514E.Seq
CAATGAAAGCATTAAAGTATATGTTACTTTGTAAAATTATGACCAACCAAACTGATGATG
TTCATGCATTAGTTAATGGTAAAATTGGTTTGAAATATCATAACAATAGATCCATTGAAG
CAATTACTCAAATTTCAAAATCTCATGCAAAGAGATCACTTCATATGTTCCAAGAGGTAA
CCAAAGAATTTTCTGATCAATTATCTGATGATCCAATCATTCATAGTCATTTAACTGAAT
TATATAGTAATCTTCTTGAACAAGATTTATGTCGTATTATTGAACCATTCTCAAGGGTTG
AAATTTCACATATTGCAAAATTAATTGATTTACCAGTTGATGTTGTTGAAAGAAAATTAT
CACTTATGATCCTTGATAAGAAATATAATGGTATCCTCGATCAAGGTACTGGTACTTTAA
TTGTATTTGAAGAACAAAAAGAAGATAAATTATTTAATTGTTCTTTAGAAACTATTGGTG
CTTTAAGTCGTGTTGTTGATTTACTTTATGAAAAAACAAACAAACTTTCTTAAATTATTT
CTTTGGTCCATCTCCCTCAATTACCAAAGATAAAAAAAAAAAAAAAAAAATATAAATAAA
AATAGAAAAACAAAC