>VSA271 (VSA271Q) /CSM/VS/VSA2-C/VSA271Q.Seq.d/
XXXXXXXXXXTAAAATCGCCACTTTTATGGGTCTCGTTTTCCCAACATTGGTAGCTGGTT
TAGGTTGGGGTGATTGGGCTGGTGGTTATTTCTACTCTGGTGTACTTCGTCTCGTTACAG
TTCAACATTCCACATTCTTGGTCAATTCATTGGCTCATTATTTAGGTGACTCTCCATACG
ATGATGAACATACTCCAAAGGATTCAGTTGTGACTGCTATATTAACATTTGGTGAAGGTT
ATCATAACTTCCATCATGAATTCCCAAATGACTATAGAAACGCTTATAAATTCTATCAAT
ATGATCCAACTAAATGGTTGATCTCTGCAATGTATTATTTAGGCTTGGCTTATGATTTGA
AAACTTTCTCCAAAAATGAAATTGAAAAAGGTATGATTCAAATGCACCAAAAACATTTAG
ACGCAAAGAAACAATCAATCTATTGGGGTATCAATAAAGAAGATTTACCATCAATGACAA
TCGATGAATTTAATAATTTGGTTG

---KIATFMGLVFPTLVAGLGWGDWAGGYFYSGVLRLVTVQHSTFLVNSLAHYLGDSPYD
DEHTPKDSVVTAILTFGEGYHNFHHEFPNDYRNAYKFYQYDPTKWLISAMYYLGLAYDLK
TFSKNEIEKGMIQMHQKHLDAKKQSIYWGINKEDLPSMTIDEFNNLV

Frame A:
---KIATFMGLVFPTLVAGLGWGDWAGGYFYSGVLRLVTVQHSTFLVNSLAHYLGDSPYD
DEHTPKDSVVTAILTFGEGYHNFHHEFPNDYRNAYKFYQYDPTKWLISAMYYLGLAYDLK
TFSKNEIEKGMIQMHQKHLDAKKQSIYWGINKEDLPSMTIDEFNNLV


Frame B:
---kspllwvsfsqhw*lv*vgviglvvistlvyfvslqfniphswsihwlii*vtlhtm
mnilqriql*lly*hlvkviitsimnsqmtietlinsinmiqlng*slqcii*awlmi*k
lspkmklkkv*fkctkni*tqrnnqsigvsikkiyhq*qsmnliiwl


Frame C:
---nrhfygsrfpnigswfrlg*lgwwlfllwctssrysstfhilgqfigslfr*lsir*
*tyskgfscdcyiniw*rls*lps*ipk*l*krl*ilsi*sn*mvdlcnvlfrlgl*fen
flqk*n*krydsnapktfrrketinllgyq*rrftindnr*i**fg

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSA271 (VSA271Q) /CSM/VS/VSA2-C/VSA271Q.Seq.d/                        979   0.0
VHQ458 (VHQ458Q) /CSM/VH/VHQ4-C/VHQ458Q.Seq.d/                        979   0.0
VHP465 (VHP465Q) /CSM/VH/VHP4-C/VHP465Q.Seq.d/                        979   0.0
VHK171 (VHK171Q) /CSM/VH/VHK1-C/VHK171Q.Seq.d/                        979   0.0
VHE438 (VHE438Q) /CSM/VH/VHE4-B/VHE438Q.Seq.d/                        979   0.0
VHE192 (VHE192Q) /CSM/VH/VHE1-D/VHE192Q.Seq.d/                        979   0.0
VHD593 (VHD593Q) /CSM/VH/VHD5-D/VHD593Q.Seq.d/                        979   0.0
VHB541 (VHB541Q) /CSM/VH/VHB5-B/VHB541Q.Seq.d/                        979   0.0
VFM428 (VFM428Q) /CSM/VF/VFM4-B/VFM428Q.Seq.d/                        979   0.0
VHD470 (VHD470Q) /CSM/VH/VHD4-C/VHD470Q.Seq.d/                        975   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.   979  0.0  1
AL033501|AL033501.1 C.albicans cosmid Ca41C10.                    72  1e-07  2
AL396412|AL396412.1 T3 end of clone AR0AA030B05 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    54  2e-07  2
BM634131|BM634131.1 17000668813253 A.Gam.ad.cDNA1 Anopheles gambiae cDNA clone 19600448419669 5', mRNA sequence.    54  8e-06  2
AQ874658|AQ874658.1 V112D8 mTn-3xHA/lacZ Insertion Library, strain Y2278 Saccharomyces cerevisiae genomic 5', genomic survey sequence.    60  3e-05  1
AR016873|AR016873.1 Sequence 1 from patent US 5777201.            60  3e-05  1
J05676|J05676.1 Saccharomyces cerevisiae delta-9 fatty acid desaturase (OLE1) gene, complete cds.    60  3e-05  1
Z72577|Z72577.1 S.cerevisiae chromosome VII reading frame ORF YGL055w.    60  3e-05  1
AL392980|AL392980.1 T3 end of clone AR0AA008A11 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    44  1e-04  2
AW335691|AW335691.1 S50D2 AGS-1 Pneumocystis carinii f. sp. carinii cDNA 3', mRNA sequence.    52  0.007  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116984_117(AC116984|pid:none) Dictyostelium discoideum chromos...   350   1e-95
AY576801_1(AY576801|pid:none) Hortaea werneckii putative delta-9...   223   2e-57
Y18554_1(Y18554|pid:none) Mortierella alpina ole2 gene for stear...   222   4e-57
AF085500_1(AF085500|pid:none) Mortierella alpina delta nine desa...   222   4e-57
AB195980_1(AB195980|pid:none) Mortierella alpina gene for omega9...   221   1e-56
Y18553_1(Y18553|pid:none) Mortierella alpina ole1 gene for stear...   221   1e-56
DQ901954_1(DQ901954|pid:none) Aureobasidium pullulans putative d...   220   1e-56
AP007162_283(AP007162|pid:none) Aspergillus oryzae RIB40 genomic...   219   3e-56
(Q12618) RecName: Full=Acyl-CoA desaturase;          EC=1.14.19....   218   6e-56
S52746(S52746;S52745) stearoyl-CoA 9-desaturase (EC 1.14.19.1) [...   216   2e-55

 psg: 0.96  gvh: 0.61  alm: 0.32  top: 0.40  tms: 0.07  mit: 0.20  mip: 0.07
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: endoplasmic reticulum
	 20.0 %: cytoplasmic
	 20.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: extracellular, including cell wall

>> prediction for VSA271 is end

>VSA271Z.Seq
----------TAAAATCGCCACTTTTATGGGTCTCGTTTTCCCAACATTGGTAGCTGGTT
TAGGTTGGGGTGATTGGGCTGGTGGTTATTTCTACTCTGGTGTACTTCGTCTCGTTACAG
TTCAACATTCCACATTCTTGGTCAATTCATTGGCTCATTATTTAGGTGACTCTCCATACG
ATGATGAACATACTCCAAAGGATTCAGTTGTGACTGCTATATTAACATTTGGTGAAGGTT
ATCATAACTTCCATCATGAATTCCCAAATGACTATAGAAACGCTTATAAATTCTATCAAT
ATGATCCAACTAAATGGTTGATCTCTGCAATGTATTATTTAGGCTTGGCTTATGATTTGA
AAACTTTCTCCAAAAATGAAATTGAAAAAGGTATGATTCAAATGCACCAAAAACATTTAG
ACGCAAAGAAACAATCAATCTATTGGGGTATCAATAAAGAAGATTTACCATCAATGACAA
TCGATGAATTTAATAATTTGGTTG