>VSA222 (VSA222Q) /CSM/VS/VSA2-A/VSA222Q.Seq.d/
XXXXXXXXXXAAGATCTTTTTAAAGGTATTGGCATTGTTAGTGATCATTGTATTTTGTGA
TTCAATTGTAAATTCATATAATATCAACAAGAAATTACATACACCAGAATTGACCGGAGC
TAAATTTGACAGACAAAATGAATATACCCGTATGTTTAGATATCAAAGAAATTCATACAT
TTGTGGTTTCTGTCTCTATTTATTCTTTTTAATCTATCGTTCACAAGGTATCATTAGTCA
ATTATCAAATGTAGAAGCCTCAAAAACTGCCATTGAAAAGCAAACTAAAAATAATTTAAA
TACCGTCGAAACTCTCTTATCGGAAAATGAAAAATTAAAAACTGAAATTAAAGACCTTAA
AAAGATGGAAAAAGAACATAAAGCTATGAAATCTCAAGCTGAAAATACAACCAAAGAATA
TCTTAAACTCCAAGAAGAATATAATCAACTTTTAGGTAAAAAACCAAAAACTCAAAAGAA
AGATGATTAATCATCTTAAAAATAATAATAATAATAATTAATTCATTATTATTATTATTA
TTATCATTATCATCATCAGATTTAAATTTATAAAAAAAAATAAATA

---IFLKVLALLVIIVFCDSIVNSYNINKKLHTPELTGAKFDRQNEYTRMFRYQRNSYIC
GFCLYLFFLIYRSQGIISQLSNVEASKTAIEKQTKNNLNTVETLLSENEKLKTEIKDLKK
MEKEHKAMKSQAENTTKEYLKLQEEYNQLLGKKPKTQKKDD*ss*k*****lihyyyyyy
hyhhqi*iykkk*i

Frame A:
---rsf*rywhc**slyfviql*ihiistrnyihqn*pelnltdkmnipvcldikeihtf
vvsvsiysf*sivhkvslvnyqm*kpqklplksklkii*ipsklsyrkmkn*klklktlk
rwkknikl*nlklkiqpknilnskkniinf*vknqklkrkminhlknnnnnn*fiiiiii
iiiiirfkfikknk


Frame B:
---dlfkgigivsdhcil*fnckfi*yqqeitytridrs*i*qtk*iypyv*iskkfihl
wflslfilfnlsftryh*siikcrslknch*kan*k*fkyrrnsligk*kikn*n*rp*k
dgkrt*syeiss*kynqris*tprri*stfr*ktknsker*liilkiiiiiinsllllll
slsssdlnl*kkin


Frame C:
---IFLKVLALLVIIVFCDSIVNSYNINKKLHTPELTGAKFDRQNEYTRMFRYQRNSYIC
GFCLYLFFLIYRSQGIISQLSNVEASKTAIEKQTKNNLNTVETLLSENEKLKTEIKDLKK
MEKEHKAMKSQAENTTKEYLKLQEEYNQLLGKKPKTQKKDD*ss*k*****lihyyyyyy
hyhhqi*iykkk*i

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSF120 (VSF120Q) /CSM/VS/VSF1-A/VSF120Q.Seq.d/                        617   e-176
VSA222 (VSA222Q) /CSM/VS/VSA2-A/VSA222Q.Seq.d/                        617   e-176
SSI190 (SSI190Q) /CSM/SS/SSI1-D/SSI190Q.Seq.d/                        617   e-176
VSB721 (VSB721Q) /CSM/VS/VSB7-A/VSB721Q.Seq.d/                        603   e-171
SSK705 (SSK705Q) /CSM/SS/SSK7-A/SSK705Q.Seq.d/                        521   e-147
SSE426 (SSE426Q) /CSM/SS/SSE4-B/SSE426Q.Seq.d/                        432   e-120
SLJ523 (SLJ523Q) /CSM/SL/SLJ5-A/SLJ523Q.Seq.d/                        430   e-119
SLH256 (SLH256Q) /CSM/SL/SLH2-C/SLH256Q.Seq.d/                        430   e-119
SSH311 (SSH311Q) /CSM/SS/SSH3-A/SSH311Q.Seq.d/                        373   e-102
VSB322 (VSB322Q) /CSM/VS/VSB3-A/VSB322Q.Seq.d/                        214   1e-54

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AL392110|AL392110.12 Human DNA sequence from clone RP11-29M23 on chromosome 9.    36  0.25  4
AF250284|AF250284.1 Amsacta moorei entomopoxvirus, complete genome.    36  0.47  5
BX538350|BX538350.1 Cryptosporidium parvum chromosome 6, complete sequence; segment 1/4.    36  0.63  6
AE014838|AE014838.1 Plasmodium falciparum 3D7 chromosome 11 section 3 of 8 of the complete sequence.    36  1.1  6
AC015537|AC015537.3 Homo sapiens chromosome 18 clone RP11-321I20 map 18, LOW-PASS SEQUENCE SAMPLING.    44  1.4  2
AL358072|AL358072.21 Human DNA sequence from clone RP11-188D8 on chromosome 1.    42  1.7  3
AP005430|AP005430.1 Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 9, PAC clone:P0705B06, WORKING DRAFT SEQUENCE, 1 ordered pieces.    44  1.9  1
AQ583480|AQ583480.1 RPCI-11-454B3.TV RPCI-11 Homo sapiens genomic clone RPCI-11-454B3, genomic survey sequence.    44  1.9  1
AE006357|AE006357.1 Lactococcus lactis subsp. lactis IL1403 section 119 of 218 of the complete genome.    44  1.9  1
AP005577|AP005577.1 Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 9, BAC clone:OJ1655_B12, WORKING DRAFT SEQUENCE, 1 ordered pieces.    44  1.9  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54K74) RecName: Full=Endoplasmic reticulum transmembrane prote...   295   5e-79
BT080943_1(BT080943|pid:none) Caligus clemensi clone ccle-evs-51...    80   5e-14
BT078760_1(BT078760|pid:none) Lepeophtheirus salmonis Pacific fo...    78   2e-13
BT076915_1(BT076915|pid:none) Caligus rogercresseyi clone crog-e...    74   4e-12
BT077060_1(BT077060|pid:none) Caligus rogercresseyi clone crog-e...    70   3e-11
BC079182_1(BC079182|pid:none) Rattus norvegicus B-cell receptor-...    70   4e-11
BT075195_1(BT075195|pid:none) Osmerus mordax clone omor-rgc-513-...    70   6e-11
BT075164_1(BT075164|pid:none) Osmerus mordax clone omor-rgc-508-...    70   6e-11
DQ525635_1(DQ525635|pid:none) Mesocricetus auratus BAP31 mRNA, c...    69   1e-10
AK008043_1(AK008043|pid:none) Mus musculus adult male small inte...    69   1e-10

 psg: 0.99  gvh: 0.66  alm: 0.39  top: 0.50  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.03  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 24.0 %: cytoplasmic
	 20.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VSA222 is nuc

>VSA222Z.Seq
----------AAGATCTTTTTAAAGGTATTGGCATTGTTAGTGATCATTGTATTTTGTGA
TTCAATTGTAAATTCATATAATATCAACAAGAAATTACATACACCAGAATTGACCGGAGC
TAAATTTGACAGACAAAATGAATATACCCGTATGTTTAGATATCAAAGAAATTCATACAT
TTGTGGTTTCTGTCTCTATTTATTCTTTTTAATCTATCGTTCACAAGGTATCATTAGTCA
ATTATCAAATGTAGAAGCCTCAAAAACTGCCATTGAAAAGCAAACTAAAAATAATTTAAA
TACCGTCGAAACTCTCTTATCGGAAAATGAAAAATTAAAAACTGAAATTAAAGACCTTAA
AAAGATGGAAAAAGAACATAAAGCTATGAAATCTCAAGCTGAAAATACAACCAAAGAATA
TCTTAAACTCCAAGAAGAATATAATCAACTTTTAGGTAAAAAACCAAAAACTCAAAAGAA
AGATGATTAATCATCTTAAAAATAATAATAATAATAATTAATTCATTATTATTATTATTA
TTATCATTATCATCATCAGATTTAAATTTATAAAAAAAAATAAATA