>VSA215 (VSA215Q) /CSM/VS/VSA2-A/VSA215Q.Seq.d/
XXXXXXXXXXTCATTCTCAACCTACATCCACAAGTTATTGAAACAAATTACTCCACCAAC
CAATGCCAAGAGTAATGAAAAAGGTGATAGAAAATTCACTATCTCATCCAAAGCCATGTC
TGTAATGAAATCCTTTGTCCACGATATCTTTGACAGAATCGCCACCGAAGCTTCTGGTTT
AGCCAAAAAGAAGAAGAGACAAACTTTACACTCAAGAGATATTCAAGTTCCCGTTCGTAT
CATTCTCACTGGTGAGTTAGCTAAACATGCCATCTTACAAGGTATGACTGCTGTCAACAA
GTACAATCCAACTGAAAGCAAAAACTAAATTGGTTCCAAATGAACTATATTCCTTAAAAA
AATATAGTTTCCAATTTTCTTTTTTTTTTGTACTCTCTCTATCATTCTTTTTTAAAAAAA
AAAAAAATC

---FSTYIHKLLKQITPPTNAKSNEKGDRKFTISSKAMSVMKSFVHDIFDRIATEASGLA
KKKKRQTLHSRDIQVPVRIILTGELAKHAILQGMTAVNKYNPTESKN*igsk*tiflkki
*fpiffffctlsiilf*kkkk

Frame A:
---hsqptstsy*nkllhqpmprvmkkvienslshpkpcl**nplstisltesppkllv*
pkrrrdklytqeifkfpfvsfslvs*lnmpsykv*llststiqlkaktklvpnelyslkk
ysfqfsfffvlslsfffkkkkn


Frame B:
---ilnlhpqvietnystnqcqe**kr**kihyliqshvcneilcpryl*qnrhrsfwfs
qkeeetnftlkrysssrsyhshw*vs*tchltrydccqqvqsn*kqklnwfqmnyip*kn
ivsnflfflyslyhsflkkkki


Frame C:
---FSTYIHKLLKQITPPTNAKSNEKGDRKFTISSKAMSVMKSFVHDIFDRIATEASGLA
KKKKRQTLHSRDIQVPVRIILTGELAKHAILQGMTAVNKYNPTESKN*igsk*tiflkki
*fpiffffctlsiilf*kkkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSA215 (VSA215Q) /CSM/VS/VSA2-A/VSA215Q.Seq.d/                        698   0.0
VSF406 (VSF406Q) /CSM/VS/VSF4-A/VSF406Q.Seq.d/                        682   0.0
VSE878 (VSE878Q) /CSM/VS/VSE8-D/VSE878Q.Seq.d/                        682   0.0
VSE742 (VSE742Q) /CSM/VS/VSE7-B/VSE742Q.Seq.d/                        682   0.0
VSB637 (VSB637Q) /CSM/VS/VSB6-B/VSB637Q.Seq.d/                        682   0.0
VSB542 (VSB542Q) /CSM/VS/VSB5-B/VSB542Q.Seq.d/                        682   0.0
VSB150 (VSB150Q) /CSM/VS/VSB1-C/VSB150Q.Seq.d/                        682   0.0
VSA778 (VSA778Q) /CSM/VS/VSA7-D/VSA778Q.Seq.d/                        682   0.0
VSA488 (VSA488Q) /CSM/VS/VSA4-D/VSA488Q.Seq.d/                        682   0.0
SSK162 (SSK162Q) /CSM/SS/SSK1-C/SSK162Q.Seq.d/                        682   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AL395901|AL395901.1 T3 end of clone AR0AA026H03 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    52  0.006  1
BM111490|BM111490.1 EST559026 potato roots Solanum tuberosum cDNA clone cPRO11D7 5' end , mRNA sequence.    38  0.041  2
BG592519|BG592519.1 EST491197 cSTS Solanum tuberosum cDNA clone cSTS1H11 5' sequence, mRNA sequence.    38  0.050  2
BG599151|BG599151.1 EST504051 cSTS Solanum tuberosum cDNA clone cSTS23B13 5' sequence, mRNA sequence.    38  0.052  2
BI920756|BI920756.1 EST540679 potato microtubers, in vitro-grown Solanum tuberosum cDNA clone cSTE26I24 5' end, mRNA sequence.    38  0.052  2
AX596878|AX596878.1 Sequence 2532 from Patent EP1258494.          38  0.13  2
Z48612|Z48612.1 S.cerevisiae chromosome IV cosmid 9934.           46  0.15  2
AI775835|AI775835.1 EST256935 tomato resistant, Cornell Lycopersicon esculentum cDNA clone cLER16P21, mRNA sequence.    36  0.15  2
BI204754|BI204754.1 EST522794 cTOS Lycopersicon esculentum cDNA clone cTOS6A3 5' end, mRNA sequence.    36  0.16  2
AW623389|AW623389.1 EST321334 tomato flower buds 3-8 mm, Cornell University Lycopersicon esculentum cDNA clone cTOB10G7 5', mRNA sequence.    36  0.17  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AJ238321_1(AJ238321|pid:none) Asellus aquaticus 5S rRNA gene, hi...   100   3e-20
EZ000286_1(EZ000286|pid:none) TSA: Amblyomma americanum Contig39...   100   3e-20
(P83863) RecName: Full=Histone H2B; Flags: Fragments;                  99   4e-20
(P19374) RecName: Full=Histone H2B;  &S11313(S11313) &X53330_2(X...    99   4e-20
BC066243_1(BC066243|pid:none) Homo sapiens histone cluster 1, H2...    99   5e-20
FB334644_1(FB334644|pid:none) Sequence 2170 from Patent WO200711...    99   5e-20
AC024696_6(AC024696|pid:none) Caenorhabditis elegans cosmid F07B...    99   5e-20
FB334560_1(FB334560|pid:none) Sequence 2086 from Patent WO200711...    99   5e-20
AM774337_2(AM774337|pid:none) Reporter vector pCH2B.                   99   5e-20
(P04255) RecName: Full=Histone H2B 1;  &AF047656_5(AF047656|pid:...    99   5e-20

 psg: 0.73  gvh: 0.47  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.00
 nuc: 0.08  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 24.0 %: mitochondrial
	 20.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for VSA215 is nuc

>VSA215Z.Seq
----------TCATTCTCAACCTACATCCACAAGTTATTGAAACAAATTACTCCACCAAC
CAATGCCAAGAGTAATGAAAAAGGTGATAGAAAATTCACTATCTCATCCAAAGCCATGTC
TGTAATGAAATCCTTTGTCCACGATATCTTTGACAGAATCGCCACCGAAGCTTCTGGTTT
AGCCAAAAAGAAGAAGAGACAAACTTTACACTCAAGAGATATTCAAGTTCCCGTTCGTAT
CATTCTCACTGGTGAGTTAGCTAAACATGCCATCTTACAAGGTATGACTGCTGTCAACAA
GTACAATCCAACTGAAAGCAAAAACTAAATTGGTTCCAAATGAACTATATTCCTTAAAAA
AATATAGTTTCCAATTTTCTTTTTTTTTTGTACTCTCTCTATCATTCTTTTTTAAAAAAA
AAAAAAATC