>AHA115 (AHA115Q) /CSM/AH/AHA1-A/AHA115Q.Seq.d/
ATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGACTTT
TATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCA
AAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAG
ATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAA
AAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTT
TCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTG
ATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCGATG
GTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTC
ACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAGTTG
AAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTACAAAG
TACTCGTCGATAACAAAGAGATCCAAGCAGGTAACCCTCGCTGATGATTGGGAATTACTX
XXXXXXXXX

iynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWHKED
GKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQKVD
CGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEINKVE
TDQLTHQYTLVISPDNTYKVLVDNKEIQAGNPR**lgi---

Frame A:
ifiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllvigikk
mvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*rmnkkl
ivvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*srkkstkl
kpin*lintlwsfhqitptkyssitkrskqvtladdwel---


Frame B:
yl*lylfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rlgikmgc**la*rr
w*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke*tks*
lwwfihqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrkepfnqernqqs*
nrsinssihfghftr*hlqstrr*qrdpsr*pslmigny---


Frame C:
iynyiffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDWESRWVVSDWHKED
GKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQKVD
CGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEINKVE
TDQLTHQYTLVISPDNTYKVLVDNKEIQAGNPR**lgi---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AHA115 (AHA115Q) /CSM/AH/AHA1-A/AHA115Q.Seq.d/                       1306   0.0
AFM264 (AFM264Q) /CSM/AF/AFM2-C/AFM264Q.Seq.d/                       1306   0.0
AFO205 (AFO205Q) /CSM/AF/AFO2-A/AFO205Q.Seq.d/                       1298   0.0
VFD485 (VFD485Q) /CSM/VF/VFD4-D/VFD485Q.Seq.d/                       1291   0.0
AFN528 (AFN528Q) /CSM/AF/AFN5-B/AFN528Q.Seq.d/                       1291   0.0
AFK286 (AFK286Q) /CSM/AF/AFK2-D/AFK286Q.Seq.d/                       1291   0.0
AFK245 (AFK245Q) /CSM/AF/AFK2-B/AFK245Q.Seq.d/                       1291   0.0
AFJ570 (AFJ570Q) /CSM/AF/AFJ5-C/AFJ570Q.Seq.d/                       1291   0.0
AFJ522 (AFJ522Q) /CSM/AF/AFJ5-A/AFJ522Q.Seq.d/                       1291   0.0
AFG846 (AFG846Q) /CSM/AF/AFG8-B/AFG846Q.Seq.d/                       1291   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.  1243   0.0    2
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92   4e-24  3
BZ392863|BZ392863.1 EINBW38TF EI_10_12_KB Entamoeba invadens genomic clone EINBW38, DNA sequence.    52   8e-07  2
BI876472|BI876472.1 fl72h01.y1 Sugano Kawakami zebrafish DRA Danio rerio cDNA clone 3819504 5' similar to TR:Q9PUC1 Q9PUC1 CALRETICULIN. ;, mRNA sequence.    42   4e-04  2
CB086220|CB086220.1 hj81f04.g1 Hedyotis centranthoides flower - Stage 2 (NYBG) Hedyotis centranthoides cDNA clone hj81f04, mRNA sequence.    36   4e-04  3
BI468042|BI468042.1 EST00452 Atlantic salmon Lambda Zap Express liver cDNA library Salmo salar cDNA clone LRR7-f02 5' similar to Calreticulin, mRNA sequence.    56   7e-04  1
CK420742|CK420742.1 AUF_IpTrk_27_j08 Trunk kidney cDNA library Ictalurus punctatus cDNA 5' similar to ER-resident chaperone calreticulin, mRNA sequence.    56   7e-04  1
AY342298|AY342298.1 Ictalurus punctatus ER-resident chaperone calreticulin mRNA, complete cds.    56   7e-04  1
CB937139|CB937139.1 IpCGJx13_12_E07_23 IpCGJx13 Ictalurus punctatus cDNA clone IpCGJx13_12_E07 5', mRNA sequence.    56   7e-04  1
AL374953|AL374953.1 Medicago truncatula EST MtBB10D04F2 : T3 end of clone MtBB10D04 of  cDNA library MtBB from symbiotic root nodules of cultivar Jemalong  of Medicago truncatula (barrel medic).    40   0.001  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   370   e-101
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   231   1e-59
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   230   2e-59
U27698_1(U27698|pid:none) Arabidopsis thaliana calreticulin (AtC...   230   3e-59
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   229   4e-59
AY836753_1(AY836753|pid:none) Triticum aestivum calreticulin-lik...   229   7e-59
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   228   1e-58
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   228   1e-58
L27348_1(L27348|pid:none) Hordeum vulgare calreticulin (CRH1) mR...   228   2e-58
AY086745_1(AY086745|pid:none) Arabidopsis thaliana clone 27210 m...   227   3e-58

 psg: 1.01  gvh: 0.69  alm: 0.49  top: 0.10  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  8.0 %: nuclear
	  4.0 %: mitochondrial
	  4.0 %: Golgi

>> prediction for AHA115 is exc

>AHA115F.Seq
ATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGACTTT
TATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTATCAACAGTTCATTTCA
AAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAGAAG
ATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATCAAA
AAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAAGTT
TCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAGTTG
ATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCGATG
GTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAGTTC
ACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAGTTG
AAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTACAAAG
TACTCGTCGATAACAAAGAGATCCAAGCAGGTAACCCTCGCTGATGATTGGGAATTACTN
NNNNNNNNN