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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO896
Library AF
(Link to library)
Clone ID AFO896
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15510-1
Original site URL
Representative seq. ID AFO896P
(Link to Original site)
Representative DNA sequence 
>AFO896 (AFO896Q) /CSM/AF/AFO8-D/AFO896Q.Seq.d/
TTGGAATAGTCCTTGGGGTGAGGGTCGTCCAGGATGGCATATTGAATGTTCAGCAATGGC
ATCCGACCTTCTTGGTGGCAATATCGATATTCATTCGGGTGGTTCAGATTTGAAATTCCC
ACATCACGATAACGAATTGGCACAATCTGAAGCATTCTATGGCAATCGTCAATGGATTAA
CTATTTCGTACACAGTGGTCATTTATTGATCGATGGTTTGAAAATGTCAAAATCCTTAAA
GAATTTCATCACAATTAAACAAGCCTTGGAAAAGTATACTTCACGTCAAATGAGAATGTT
TTTCATTCTTCATAAATATGATAAAGCCATGAACTATTCACCAGAGTCCATGGGTTACGC
CATTGAAATGGAGAAAACTTTTGTAGGGTTCTTCCATACCGCCAAACAAATCCTTAGAGA
TTCTCCATTATCTTTACCACAATTTTGGACTCAAGCTGAAAAGGATTTAAATAAACATCT
TCAAAATGCAAATGACCAAGTTCATCAATTCATTCTTGATAACTTTAACACCTCTGACGC
TTTGAAAACTTTATCAGATCTCGTCAATAAAAXXXXXXXXXXGTTTACATTAGATCATGT
GCTGANCAAAAGGACAAATCCAAGATTAAATTTAATTAGTGCAATCGCAGAGTATATCAC
TTACATTTTCTCTGTTTTCGGTTTAACCGAATCTTCAACCGCCTCTTCAATGATTGGTTT
CGGTTCCGCTGGTAAAGGTAATATCGAAGAGGAGATGACTCCAATCTTAAATGCTCTCAC
TCAATTCCGTTCAGAGGTACGTGCTTCTGCAATCGCAAAAGATACTACCTCAATCCTTAA
AACTTGTGATAACCTTCGTGATGAGGTTTTACCATTATTGGGCGTAAAGATTGATGATAA
ATCCGCAACAACTGCAATGTGGAAATTTGAAGATAAAGAAACCCTCAAAAAAGAAATTGA
ACAAAAGAAAGAAATTGAAAAGAAGAAACAAGCTGACAAGGAAGAAAAAGAAAAAAAATT
AAAAGAGAAATTTGAAAAATCAAAAATTCCACCACAACAACTCTTTATCAATGAAACTGA
TAAATACTCAAAATTCAATGAATTGGGTATGCCAACTCATGATAAGGAAGGTGTTGAAAT
TACAAAATCACAATTAAAAAAATTACAAAAAGAATATGATAATCAAACTAAAGAACATAA
TAATTATTTGAAATCATTATCAACTTCAACATCATCACCAACTTTAACTTCAACTCAATC
ACCACAATAAAATTATTTTAAAATAAAATAAAATAAAATAAAATAAAATAAAATAAAAAA
TAAAAAAACAAAATATATTATTTTTTT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
WNSPWGEGRPGWHIECSAMASDLLGGNIDIHSGGSDLKFPHHDNELAQSEAFYGNRQWIN
YFVHSGHLLIDGLKMSKSLKNFITIKQALEKYTSRQMRMFFILHKYDKAMNYSPESMGYA
IEMEKTFVGFFHTAKQILRDSPLSLPQFWTQAEKDLNKHLQNANDQVHQFILDNFNTSDA
LKTLSDLVNK---

---FTLDHVLXKRTNPRLNLISAIAEYITYIFSVFGLTESSTASSMIGFGSAGKGNIEEE
MTPILNALTQFRSEVRASAIAKDTTSILKTCDNLRDEVLPLLGVKIDDKSATTAMWKFED
KETLKKEIEQKKEIEKKKQADKEEKEKKLKEKFEKSKIPPQQLFINETDKYSKFNELGMP
THDKEGVEITKSQLKKLQKEYDNQTKEHNNYLKSLSTSTSSPTLTSTQSPQ*nyfkik*n
kik*nkiknkktkyiif
Translated Amino Acid sequence (All Frames) 
Frame A:
le*slg*gssrmay*mfsngirpswwqyrysfgwfrfeiptsr*rigti*silwqssmd*
lfrtqwsfidrwfenvkilkefhhn*tslgkvyftsnenvfhss*i**shelftrvhglr
h*ngenfcrvlpyrqtnp*rfsiifttildss*kgfk*tsskck*psssihs**l*hl*r
fenfirsrq*---

---vyirscaxqkdkskikfn*cnrrvyhlhflcfrfnrifnrlfndwfrfrw*r*yrrg
ddsnlkcshsipfrgtcfcnrkryylnp*nl**ps**gftiigrkd***irnncnvei*r
*rnpqkrn*tkern*keets*qgrkrkkikrei*kiknsttttlyq*n**ilkiq*igya
ns**grc*nykitikkitkri**sn*rt**lfeiiinfniitnfnfnsittiklf*nkik
*nkik*nkk*knkiyyff

Frame B:
WNSPWGEGRPGWHIECSAMASDLLGGNIDIHSGGSDLKFPHHDNELAQSEAFYGNRQWIN
YFVHSGHLLIDGLKMSKSLKNFITIKQALEKYTSRQMRMFFILHKYDKAMNYSPESMGYA
IEMEKTFVGFFHTAKQILRDSPLSLPQFWTQAEKDLNKHLQNANDQVHQFILDNFNTSDA
LKTLSDLVNK---

---FTLDHVLXKRTNPRLNLISAIAEYITYIFSVFGLTESSTASSMIGFGSAGKGNIEEE
MTPILNALTQFRSEVRASAIAKDTTSILKTCDNLRDEVLPLLGVKIDDKSATTAMWKFED
KETLKKEIEQKKEIEKKKQADKEEKEKKLKEKFEKSKIPPQQLFINETDKYSKFNELGMP
THDKEGVEITKSQLKKLQKEYDNQTKEHNNYLKSLSTSTSSPTLTSTQSPQ*nyfkik*n
kik*nkiknkktkyiif

Frame C:
givlgvrvvqdgilnvqqwhptflvaisifirvvqi*nshititnwhnlkhsmaivnglt
isytvviy*smv*kcqnp*rissqlnkpwksilhvk*ecfsffinmikp*tihqspwvtp
lkwrkll*gssippnksleilhylyhnfglklkri*inifkmqmtkfinsflitltpltl
*klyqissik---

---lh*imc*xkgqiqd*i*lvqsqsisltfslfsv*pnlqpplq*lvsvplvkviskrr
*lqs*mlslnsvqryvllqsqkilpqslklvitfvmrfyhywa*rlminpqqlqcgnlki
kkpskkklnkrkklkrrnkltrkkkkkn*krnlknqkfhhnnslsmklintqnsmnwvcq
lmirkvlklqnhn*knykknmiiklkniiii*nhyqlqhhhql*lqlnhhnkiilk*nki
k*nkik*kikkqnilff
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO896 (AFO896Q) /CSM/AF/AFO8-D/AFO896Q.Seq.d/                       1774   0.0
SFD396 (SFD396Q) /CSM/SF/SFD3-D/SFD396Q.Seq.d/                       1304   0.0
AFO752 (AFO752Q) /CSM/AF/AFO7-C/AFO752Q.Seq.d/                       1277   0.0
SFI454 (SFI454Q) /CSM/SF/SFI4-C/SFI454Q.Seq.d/                       1259   0.0
AFO629 (AFO629Q) /CSM/AF/AFO6-B/AFO629Q.Seq.d/                       1199   0.0
AFM118 (AFM118Q) /CSM/AF/AFM1-A/AFM118Q.Seq.d/                       1183   0.0
VFL754 (VFL754Q) /CSM/VF/VFL7-C/VFL754Q.Seq.d/                       1164   0.0
CHD303 (CHD303Q) /CSM/CH/CHD3-A/CHD303Q.Seq.d/                       1154   0.0
SFH371 (SFH371Q) /CSM/SF/SFH3-C/SFH371Q.Seq.d/                        975   0.0
AFF277 (AFF277Q) /CSM/AF/AFF2-D/AFF277Q.Seq.d/                        894   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

Z71523|Z71523.1 S.cerevisiae chromosome XIV reading frame ORF YNL247w.    52  2e-14  4
AX489141|AX489141.1 Sequence 6441 from Patent WO02053728.         56  1e-12  3
X96722|X96722.1 S.cerevisiae DNA region from chromosome XIV, left arm.    52  1e-11  4
BM393975|BM393975.1 50072-2-12-C03.f.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    56  8e-11  2
BM392731|BM392731.1 50071-2-12-C03.f.1 Chilcoat/Turkewitz cDNA (small fraction) Tetrahymena thermophila cDNA, mRNA sequence.    56  8e-11  2
BM401376|BM401376.1 5009-0-9-G01.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.    50  4e-09  2
A93879|A93879.1 Sequence 1 from Patent WO9726341.                 50  1e-07  2
A64016|A64016.2 Sequence 1 from Patent EP0785257.                 50  1e-07  2
AR100592|AR100592.1 Sequence 1 from patent US 6080717.            50  1e-07  2
AR016529|AR016529.1 Sequence 1 from patent US 5776749.            50  1e-07  2
dna update 2003. 9.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q5F408) RecName: Full=Cysteinyl-tRNA synthetase, cytoplasmic;  ...   311   3e-83
(Q4R550) RecName: Full=Cysteinyl-tRNA synthetase, cytoplasmic;  ...   307   5e-82
BC114705_1(BC114705|pid:none) Bos taurus cysteinyl-tRNA syntheta...   306   1e-81
AY892196_1(AY892196|pid:none) Synthetic construct Homo sapiens c...   305   3e-81
(P49589) RecName: Full=Cysteinyl-tRNA synthetase, cytoplasmic;  ...   305   3e-81
(Q7KN90) RecName: Full=Cysteinyl-tRNA synthetase, cytoplasmic;  ...   303   1e-80
(Q9ER72) RecName: Full=Cysteinyl-tRNA synthetase, cytoplasmic;  ...   302   2e-80
BC030473_1(BC030473|pid:none) Mus musculus cysteinyl-tRNA synthe...   302   2e-80
(Q5M7N8) RecName: Full=Cysteinyl-tRNA synthetase, cytoplasmic;  ...   300   1e-79
AK135433_1(AK135433|pid:none) Mus musculus 12 days embryo female...   299   2e-79
protein update 2009. 4.26
PSORT 

 psg: 0.73  gvh: 0.44  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.06  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 24.0 %: nuclear
	  8.0 %: vesicles of secretory system
	  4.0 %: mitochondrial
	  4.0 %: Golgi

>> prediction for AFO896 is cyt
5' end seq. ID AFO896F
5' end seq. 
>AFO896F.Seq
TTGGAATAGTCCTTGGGGTGAGGGTCGTCCAGGATGGCATATTGAATGTTCAGCAATGGC
ATCCGACCTTCTTGGTGGCAATATCGATATTCATTCGGGTGGTTCAGATTTGAAATTCCC
ACATCACGATAACGAATTGGCACAATCTGAAGCATTCTATGGCAATCGTCAATGGATTAA
CTATTTCGTACACAGTGGTCATTTATTGATCGATGGTTTGAAAATGTCAAAATCCTTAAA
GAATTTCATCACAATTAAACAAGCCTTGGAAAAGTATACTTCACGTCAAATGAGAATGTT
TTTCATTCTTCATAAATATGATAAAGCCATGAACTATTCACCAGAGTCCATGGGTTACGC
CATTGAAATGGAGAAAACTTTTGTAGGGTTCTTCCATACCGCCAAACAAATCCTTAGAGA
TTCTCCATTATCTTTACCACAATTTTGGACTCAAGCTGAAAAGGATTTAAATAAACATCT
TCAAAATGCAAATGACCAAGTTCATCAATTCATTCTTGATAACTTTAACACCTCTGACGC
TTTGAAAACTTTATCAGATCTCGTCAATAAAA----------
Length of 5' end seq. 572
3' end seq. ID AFO896Z
3' end seq. 
>AFO896Z.Seq
----------GTTTACATTAGATCATGTGCTGANCAAAAGGACAAATCCAAGATTAAATT
TAATTAGTGCAATCGCAGAGTATATCACTTACATTTTCTCTGTTTTCGGTTTAACCGAAT
CTTCAACCGCCTCTTCAATGATTGGTTTCGGTTCCGCTGGTAAAGGTAATATCGAAGAGG
AGATGACTCCAATCTTAAATGCTCTCACTCAATTCCGTTCAGAGGTACGTGCTTCTGCAA
TCGCAAAAGATACTACCTCAATCCTTAAAACTTGTGATAACCTTCGTGATGAGGTTTTAC
CATTATTGGGCGTAAAGATTGATGATAAATCCGCAACAACTGCAATGTGGAAATTTGAAG
ATAAAGAAACCCTCAAAAAAGAAATTGAACAAAAGAAAGAAATTGAAAAGAAGAAACAAG
CTGACAAGGAAGAAAAAGAAAAAAAATTAAAAGAGAAATTTGAAAAATCAAAAATTCCAC
CACAACAACTCTTTATCAATGAAACTGATAAATACTCAAAATTCAATGAATTGGGTATGC
CAACTCATGATAAGGAAGGTGTTGAAATTACAAAATCACAATTAAAAAAATTACAAAAAG
AATATGATAATCAAACTAAAGAACATAATAATTATTTGAAATCATTATCAACTTCAACAT
CATCACCAACTTTAACTTCAACTCAATCACCACAATAAAATTATTTTAAAATAAAATAAA
ATAAAATAAAATAAAATAAAATAAAAAATAAAAAAACAAAATATATTATTTTTTT
Length of 3' end seq. 765
Connected seq. ID AFO896P
Connected seq. 
>AFO896P.Seq
TTGGAATAGTCCTTGGGGTGAGGGTCGTCCAGGATGGCATATTGAATGTTCAGCAATGGC
ATCCGACCTTCTTGGTGGCAATATCGATATTCATTCGGGTGGTTCAGATTTGAAATTCCC
ACATCACGATAACGAATTGGCACAATCTGAAGCATTCTATGGCAATCGTCAATGGATTAA
CTATTTCGTACACAGTGGTCATTTATTGATCGATGGTTTGAAAATGTCAAAATCCTTAAA
GAATTTCATCACAATTAAACAAGCCTTGGAAAAGTATACTTCACGTCAAATGAGAATGTT
TTTCATTCTTCATAAATATGATAAAGCCATGAACTATTCACCAGAGTCCATGGGTTACGC
CATTGAAATGGAGAAAACTTTTGTAGGGTTCTTCCATACCGCCAAACAAATCCTTAGAGA
TTCTCCATTATCTTTACCACAATTTTGGACTCAAGCTGAAAAGGATTTAAATAAACATCT
TCAAAATGCAAATGACCAAGTTCATCAATTCATTCTTGATAACTTTAACACCTCTGACGC
TTTGAAAACTTTATCAGATCTCGTCAATAAAA----------GTTTACATTAGATCATGT
GCTGANCAAAAGGACAAATCCAAGATTAAATTTAATTAGTGCAATCGCAGAGTATATCAC
TTACATTTTCTCTGTTTTCGGTTTAACCGAATCTTCAACCGCCTCTTCAATGATTGGTTT
CGGTTCCGCTGGTAAAGGTAATATCGAAGAGGAGATGACTCCAATCTTAAATGCTCTCAC
TCAATTCCGTTCAGAGGTACGTGCTTCTGCAATCGCAAAAGATACTACCTCAATCCTTAA
AACTTGTGATAACCTTCGTGATGAGGTTTTACCATTATTGGGCGTAAAGATTGATGATAA
ATCCGCAACAACTGCAATGTGGAAATTTGAAGATAAAGAAACCCTCAAAAAAGAAATTGA
ACAAAAGAAAGAAATTGAAAAGAAGAAACAAGCTGACAAGGAAGAAAAAGAAAAAAAATT
AAAAGAGAAATTTGAAAAATCAAAAATTCCACCACAACAACTCTTTATCAATGAAACTGA
TAAATACTCAAAATTCAATGAATTGGGTATGCCAACTCATGATAAGGAAGGTGTTGAAAT
TACAAAATCACAATTAAAAAAATTACAAAAAGAATATGATAATCAAACTAAAGAACATAA
TAATTATTTGAAATCATTATCAACTTCAACATCATCACCAACTTTAACTTCAACTCAATC
ACCACAATAAAATTATTTTAAAATAAAATAAAATAAAATAAAATAAAATAAAATAAAAAA
TAAAAAAACAAAATATATTATTTTTTT
Length of connected seq. 1337
Full length Seq ID -
Full length Seq. -
Length of full length seq. -