>AFO894 (AFO894Q) /CSM/AF/AFO8-D/AFO894Q.Seq.d/
CACTGTTGGCCTACTGGTANTTCATGCATATTNATAATTATATNTTTTTTGTTTAATAAA
AAAGAAAGAATACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCA
ATTTTGCATTATCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGGXXXXXXXXX
XCAGTCGATTGGGTTGACGTAAAGNNAATCGATGACCCAGAAGATGTTAAACCAGCCGGT
CATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAAT
GAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAA
TGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACACCCATTAATT
GATAACCCAGAATNCGCTGAAGACAATGAACTNTACCTCTTTAATGATCTCGGTGCTATC
GGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCACCGAC
TCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGNTGCCAACCAAGAAGCCGAA
AAGAAAATGTTTGNCGNCCTCNAAGCCGCCAAAGC

llaywxfmhixnyxffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDW---

---VDWVDVKXIDDPEDVKPAGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYK
GEWKAKKIPNPEYKGEWVHPLIDNPEXAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIV
TDSVEEAKDFSEKTFXANQEAEKKMFXXLXAAK

Frame A:
hcwptgxscixiiixflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitig---

---qsiglt*xxsmtqkmlnqpvmtisqlqsliqklsnqrigmkkttvngkpqpspiqnt
kvngkqrksqiqntkvngyth*litqnxlktmnxtslmisvlsvsnygklnlvpslti*s
sptlskklkislkrplxptkkpkrkclxxskppk

Frame B:
tvgllvxhayx*lyxfclikkkeyqhetfivfnflsfcfqfciinssfqryl**rl---

---srlg*rkxnr*prrc*tsrs*rypsfnr*srscqtrgle*rrrr*mgspnhrqsriq
r*meskenpksriqr*mgtpin**prxr*rq*txpl**srcyrfrimas*iwfhl*qydr
hrlcrrs*rfl*kdlxcqprsrkenvxrpxsrqs

Frame C:
llaywxfmhixnyxffv**KRKNTNMRLLLCLIFLVFVFNFALSTVHFKDTFDNDW---

---VDWVDVKXIDDPEDVKPAGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYK
GEWKAKKIPNPEYKGEWVHPLIDNPEXAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIV
TDSVEEAKDFSEKTFXANQEAEKKMFXXLXAAK

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFM129 (VFM129Q) /CSM/VF/VFM1-B/VFM129Q.Seq.d/                        852   0.0
VFL130 (VFL130Q) /CSM/VF/VFL1-B/VFL130Q.Seq.d/                        852   0.0
VFK631 (VFK631Q) /CSM/VF/VFK6-B/VFK631Q.Seq.d/                        852   0.0
VFK516 (VFK516Q) /CSM/VF/VFK5-A/VFK516Q.Seq.d/                        852   0.0
VFI619 (VFI619Q) /CSM/VF/VFI6-A/VFI619Q.Seq.d/                        852   0.0
VFG242 (VFG242Q) /CSM/VF/VFG2-B/VFG242Q.Seq.d/                        852   0.0
VFE283 (VFE283Q) /CSM/VF/VFE2-D/VFE283Q.Seq.d/                        852   0.0
VFE122 (VFE122Q) /CSM/VF/VFE1-A/VFE122Q.Seq.d/                        852   0.0
VFD449 (VFD449Q) /CSM/VF/VFD4-C/VFD449Q.Seq.d/                        852   0.0
VFC302 (VFC302Q) /CSM/VF/VFC3-A/VFC302Q.Seq.d/                        852   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.   852  0.0  3
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80  6e-12  2
AZ543757|AZ543757.1 ENTGD24TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80  4e-11  1
AW649196|AW649196.1 EST327650 tomato germinating seedlings, TAMU Lycopersicon esculentum cDNA clone cLEI7M10 5', mRNA sequence.    74  2e-09  1
AW626316|AW626316.1 EST320223 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ19K24 5', mRNA sequence.    74  2e-09  1
BE459798|BE459798.1 EST415090 tomato developing/immature green fruit Lycopersicon esculentum cDNA clone cLEM8G13, mRNA sequence.    74  2e-09  1
AI771812|AI771812.1 EST252912 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED38E1, mRNA sequence.    74  2e-09  1
BE435540|BE435540.1 EST406618 tomato breaker fruit, TIGR Lycopersicon esculentum cDNA clone cLEG27F24, mRNA sequence.    74  2e-09  1
AI782264|AI782264.1 EST263143 tomato susceptible, Cornell Lycopersicon esculentum cDNA clone cLES18L5, mRNA sequence.    74  2e-09  1
AW932476|AW932476.1 EST358319 tomato fruit mature green, TAMU Lycopersicon esculentum cDNA clone cLEF48O8 5', mRNA sequence.    74  2e-09  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   249   6e-65
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   155   7e-37
(Q9STD3) RecName: Full=Calreticulin; Flags: Precursor;  &AJ00076...   155   9e-37
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   154   2e-36
AM445600_2(AM445600|pid:none) Vitis vinifera contig VV78X277184....   152   6e-36
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   152   7e-36
AK230218_1(AK230218|pid:none) Arabidopsis thaliana mRNA for hypo...   152   7e-36
AC149080_5(AC149080|pid:none) Medicago truncatula clone mth2-75j...   151   2e-35
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   150   2e-35
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   150   4e-35

 psg: 1.01  gvh: 0.69  alm: 0.47  top: -0.03  tms: 0.00  mit: 0.40  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: extracellular, including cell wall
	 24.0 %: cytoplasmic
	 12.0 %: vacuolar
	 12.0 %: nuclear
	 12.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system

>> prediction for AFO894 is exc

>AFO894F.Seq
CACTGTTGGCCTACTGGTANTTCATGCATATTNATAATTATATNTTTTTTGTTTAATAAA
AAAGAAAGAATACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCA
ATTTTGCATTATCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGG---------
-

>AFO894Z.Seq
----------CAGTCGATTGGGTTGACGTAAAGNNAATCGATGACCCAGAAGATGTTAAA
CCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAG
GATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATAC
AAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACAC
CCATTAATTGATAACCCAGAATNCGCTGAAGACAATGAACTNTACCTCTTTAATGATCTC
GGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATC
GTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGNTGCCAACCAA
GAAGCCGAAAAGAAAATGTTTGNCGNCCTCNAAGCCGCCAAAGC

>AFO894P.Seq
CACTGTTGGCCTACTGGTANTTCATGCATATTNATAATTATATNTTTTTTGTTTAATAAA
AAAGAAAGAATACCAACATGAGACTTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCA
ATTTTGCATTATCAACAGTTCATTTCAAAGATACCTTTGATAACGATTGGG---------
-CAGTCGATTGGGTTGACGTAAAGNNAATCGATGACCCAGAAGATGTTAAACCAGCCGGT
CATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGAAT
GAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTGAA
TGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACACCCATTAATT
GATAACCCAGAATNCGCTGAAGACAATGAACTNTACCTCTTTAATGATCTCGGTGCTATC
GGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCACCGAC
TCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGNTGCCAACCAAGAAGCCGAA
AAGAAAATGTTTGNCGNCCTCNAAGCCGCCAAAGC