>AFO890 (AFO890Q) /CSM/AF/AFO8-D/AFO890Q.Seq.d/
ATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGAC
TTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTACCAACAGTTCATT
TCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAG
AAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATC
AAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAA
GTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAG
TTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCG
ATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAG
TTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAG
TTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTACA
AAGTACTCGTCGATAACAAAGAGATXXXXXXXXXXTTTCACCAGATAACACCTACAAAGT
ACTCGTCGATAACAAAGAGATCCAAGCAGGGTAACCTCGCTGATGATTGGGAATTACTTC
CATCAAAACAAATCAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAGG
AAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCG
TTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAG
CCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATC
CAGAATACAAAGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACA
ATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAAT
CTGGTTCCATCTTTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCT
CTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAG
CCGCCAAAGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTG
AAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTA
AAACTGAGACAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGTNATCAGACGAACTTT
A

hiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALPTVHFKDTFDNDWESRWVVSDWHKE
DGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQKV
DCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEINKV
ETDQLTHQYTLVISPDNTYKVLVDNKE---

---FHQITPTKYSSITKRSKQGNLADDWELLPSKQIKDPKQSKPVDWVDVKEIDDPEDVK
PAGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVH
PLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQ
EAEKKMFDDLEAAKAEERKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVKTETKKKKS
KNQPKKXSDEL

Frame A:
iifiiisflfnkkeript*dfycv*fs*flfsilhyqqfiskiplitignqdgllvigik
kmvnqvn*filqvnglvmkikkvfklvkmlvstlyqlnsqvsvtkvki*fsntl*rmnkk
livvvhtssyyhpn*inqhsmvnlniplclvqmfvvpqreftsfsttkerti*srkkstk
lkpin*lintlwsfhqitptkyssitkr---

---FHQITPTKYSSITKRSKQGNLADDWELLPSKQIKDPKQSKPVDWVDVKEIDDPEDVK
PAGHDDIPASIVDPEAVKPEDWNEEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVH
PLIDNPEYAEDNELYLFNDLGAIGFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQ
EAEKKMFDDLEAAKAEERKKADEKLAAEKAAEKEAEEADEEEEEVAEEDLVKTETKKKKS
KNQPKKXSDEL

Frame B:
syl*lylfclikkkeyqhetfivfnflsfcfqfcitnssfqryl**rlgikmgc**la*r
rw*ir*issycr*mvw**kskrysn**rcsflrcis*ipkfq*qr*rfsspihcke*tks
*lwwfihqvitiqirsisirw*i*ifhyvwsrclwclkessrhsqlqrkepfnqernqqs
*nrsinssihfghftr*hlqstrr*qrd---

---ftr*hlqstrr*qrdpsrvtslmignyfhqnkskiqnnpnqsiglt*rksmtqkmln
qpvmtisqlqsliqklsnqrigmkkttvngkpqpspiqntkvngkqrksqiqntkvngyt
h*litqntlktmnstslmisvlsvsnygklnlvpslti*ssptlskklkislkrpllptk
kpkrkclttskppklkrerkltkn*lpkkllkkkpkklmkkkkkslkki*lklrqkrrsq
kinqksxqtnf

Frame C:
hiynyiffv**KRKNTNMRLLLCLIFLVFVFNFALPTVHFKDTFDNDWESRWVVSDWHKE
DGKSGKLVHTAGKWFGDENQKGIQTSEDARFYAVSAKFPSFSNKGKDLVLQYTVKNEQKV
DCGGSYIKLLPSKLDQSAFDGESEYSIMFGPDVCGASKRVHVILNYKGKNHLIKKEINKV
ETDQLTHQYTLVISPDNTYKVLVDNKE---

---spdntykvlvdnkeiqag*pr**lgitsiktnqrsktiqtsrlg*rkgnr*prrc*t
srs*rypsfnr*srscqtrgle*rrrr*mgspnhrqsriqr*meskenpksriqr*mgtp
in**prir*rq*tlpl**srcyrfrimas*iwfhl*qydrhrlcrrs*rfl*kdlccqpr
srkenv*rprsrqs*rekes*rkiscrksc*krsrrs**rrrrsr*rrfs*n*dkkeevk
kstkkvirrtl

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO890 (AFO890Q) /CSM/AF/AFO8-D/AFO890Q.Seq.d/                       2351   0.0
VFH144 (VFH144Q) /CSM/VF/VFH1-B/VFH144Q.Seq.d/                       1231   0.0
AFO559 (AFO559Q) /CSM/AF/AFO5-C/AFO559Q.Seq.d/                       1231   0.0
AFN554 (AFN554Q) /CSM/AF/AFN5-C/AFN554Q.Seq.d/                       1231   0.0
AFN528 (AFN528Q) /CSM/AF/AFN5-B/AFN528Q.Seq.d/                       1231   0.0
AFM264 (AFM264Q) /CSM/AF/AFM2-C/AFM264Q.Seq.d/                       1231   0.0
AFK286 (AFK286Q) /CSM/AF/AFK2-D/AFK286Q.Seq.d/                       1231   0.0
AFK245 (AFK245Q) /CSM/AF/AFK2-B/AFK245Q.Seq.d/                       1231   0.0
AFJ570 (AFJ570Q) /CSM/AF/AFJ5-C/AFJ570Q.Seq.d/                       1231   0.0
AFJ522 (AFJ522Q) /CSM/AF/AFJ5-A/AFJ522Q.Seq.d/                       1231   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.  1207  0.0  2
BD248385|BD248385.1 Gene participating in tolerance against environmental stress.    92  4e-28  4
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80  1e-21  4
AZ543757|AZ543757.1 ENTGD24TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80  8e-11  1
BI921109|BI921109.1 EST541012 tomato callus Lycopersicon esculentum cDNA clone cLEC72A21 5' end, mRNA sequence.    74  9e-11  2
AW649196|AW649196.1 EST327650 tomato germinating seedlings, TAMU Lycopersicon esculentum cDNA clone cLEI7M10 5', mRNA sequence.    74  5e-09  1
AW626316|AW626316.1 EST320223 tomato radicle, 5 d post-imbibition, Cornell University Lycopersicon esculentum cDNA clone cLEZ19K24 5', mRNA sequence.    74  5e-09  1
BE459798|BE459798.1 EST415090 tomato developing/immature green fruit Lycopersicon esculentum cDNA clone cLEM8G13, mRNA sequence.    74  5e-09  1
AI771812|AI771812.1 EST252912 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED38E1, mRNA sequence.    74  5e-09  1
BE435540|BE435540.1 EST406618 tomato breaker fruit, TIGR Lycopersicon esculentum cDNA clone cLEG27F24, mRNA sequence.    74  5e-09  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   662   0.0
(Q40401) RecName: Full=Calreticulin; Flags: Precursor;  &T16968(...   401   e-110
AC133339_28(AC133339|pid:none) Oryza sativa chromosome 3 BAC OSJ...   401   e-110
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   401   e-110
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   400   e-110
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   400   e-110
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   400   e-110
EU984501_1(EU984501|pid:none) Nicotiana tabacum calreticulin mRN...   400   e-110
AY836753_1(AY836753|pid:none) Triticum aestivum calreticulin-lik...   398   e-109
EF084103_1(EF084103|pid:none) Picea sitchensis clone WS0274_F04 ...   396   e-109

 psg: 1.01  gvh: 0.64  alm: 0.18  top: 0.10  tms: 0.07  mit: 0.39  mip: 0.03
 nuc: 0.10  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 36.0 %: mitochondrial
	 20.0 %: Golgi
	 16.0 %: cytoplasmic
	 12.0 %: nuclear
	  8.0 %: endoplasmic reticulum
	  4.0 %: vacuolar
	  4.0 %: extracellular, including cell wall

>> prediction for AFO890 is mit

>AFO890F.Seq
ATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGAC
TTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTACCAACAGTTCATT
TCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAG
AAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATC
AAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAA
GTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAG
TTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCG
ATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAG
TTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAG
TTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTACA
AAGTACTCGTCGATAACAAAGAGAT----------

>AFO890Z.Seq
----------TTTCACCAGATAACACCTACAAAGTACTCGTCGATAACAAAGAGATCCAA
GCAGGGTAACCTCGCTGATGATTGGGAATTACTTCCATCAAAACAAATCAAAGATCCAAA
ACAATCCAAACCAGTCGATTGGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGTTAA
ACCAGCCGGTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGA
GGATTGGAATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATA
CAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACA
CCCATTAATTGATAACCCAGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCT
CGGTGCTATCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGAT
CGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGTTGCCAACCA
AGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAA
AGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTGAAAAAGAAGCCGAAGAAGCTGATGA
AGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTAAAACTGAGACAAAAAAGAAGAAGTC
AAAAAATCAACCAAAAAAGTNATCAGACGAACTTTA

>AFO890P.Seq
ATCATATTTATAATTATATCTTTTTTGTTTAATAAAAAAGAAAGAATACCAACATGAGAC
TTTTATTGTGTTTAATTTTCTTAGTTTTTGTTTTCAATTTTGCATTACCAACAGTTCATT
TCAAAGATACCTTTGATAACGATTGGGAATCAAGATGGGTTGTTAGTGATTGGCATAAAG
AAGATGGTAAATCAGGTAAATTAGTTCATACTGCAGGTAAATGGTTTGGTGATGAAAATC
AAAAAGGTATTCAAACTAGTGAAGATGCTCGTTTCTACGCTGTATCAGCTAAATTCCCAA
GTTTCAGTAACAAAGGTAAAGATTTAGTTCTCCAATACACTGTAAAGAATGAACAAAAAG
TTGATTGTGGTGGTTCATACATCAAGTTATTACCATCCAAATTAGATCAATCAGCATTCG
ATGGTGAATCTGAATATTCCATTATGTTTGGTCCAGATGTTTGTGGTGCCTCAAAGAGAG
TTCACGTCATTCTCAACTACAAAGGAAAGAACCATTTAATCAAGAAAGAAATCAACAAAG
TTGAAACCGATCAATTAACTCATCAATACACTTTGGTCATTTCACCAGATAACACCTACA
AAGTACTCGTCGATAACAAAGAGAT----------TTTCACCAGATAACACCTACAAAGT
ACTCGTCGATAACAAAGAGATCCAAGCAGGGTAACCTCGCTGATGATTGGGAATTACTTC
CATCAAAACAAATCAAAGATCCAAAACAATCCAAACCAGTCGATTGGGTTGACGTAAAGG
AAATCGATGACCCAGAAGATGTTAAACCAGCCGGTCATGACGATATCCCAGCTTCAATCG
TTGATCCAGAAGCTGTCAAACCAGAGGATTGGAATGAAGAAGACGACGGTGAATGGGAAG
CCCCAACCATCGCCAATCCAGAATACAAAGGTGAATGGAAAGCAAAGAAAATCCCAAATC
CAGAATACAAAGGTGAATGGGTACACCCATTAATTGATAACCCAGAATACGCTGAAGACA
ATGAACTCTACCTCTTTAATGATCTCGGTGCTATCGGTTTCGAATTATGGCAAGTTAAAT
CTGGTTCCATCTTTAACAATATGATCGTCACCGACTCTGTCGAAGAAGCTAAAGATTTCT
CTGAAAAGACCTTTGTTGCCAACCAAGAAGCCGAAAAGAAAATGTTTGACGACCTCGAAG
CCGCCAAAGCTGAAGAGAGAAAGAAAGCTGACGAAAAATTAGCTGCCGAAAAAGCTGCTG
AAAAAGAAGCCGAAGAAGCTGATGAAGAAGAAGAAGAAGTCGCTGAAGAAGATTTAGTTA
AAACTGAGACAAAAAAGAAGAAGTCAAAAAATCAACCAAAAAAGTNATCAGACGAACTTT
A