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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHQ262
Library VH
(Link to library)
Clone ID VHQ262
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16429-1
Original site URL
Representative seq. ID VHQ262P
(Link to Original site)
Representative DNA sequence 
>VHQ262 (VHQ262Q) /CSM/VH/VHQ2-C/VHQ262Q.Seq.d/
CTTAAAGATGACACAAATAATCCATTTGATAAAGTAAAGGATGTATTAAAATCCAAGATC
AAACTTATAATTTTTATAATCTTTCAAAACTTCAAGACCCAAGAATTGAAAAATTACCAT
ACTCCATTAGAATTCTTTTGGAATCAGCAGTTCGTAATTGTGATAACTTTGAAGTACACG
AAAAAGATGTTGAAAATATCTTAAATTGGGAAAACACTGCAAATAAAGTTGAAATTCCTT
TCAAACCAGCAAGAGTATTATTACAAGATTTTACTGGTGTACCAGCAGTTGTTGATCTTG
CAGCAATGAGAGATGCCATGAAAAGATTAGGTGGTGATCCAGCAAAAATTAACCCACTTG
TTCCAGTTGATTTAGTTATTGATCACTCTGTTCAAGTTGATGTATCAAGAACTGTTGATG
CTTTAGAGCAAAATCAAAAGATTGAATTCCATAGAAATCATGAAAGATTTTCATTTTTAA
AATGGGGTGCTCAAGCATTTGATGGATTATTTATTGCTCCACCTGGAAGTGGTATTGTAC
ATCAAGTAAATTTAGAGTATATTGCAAGAGAAGTTATGAATGGAACTGGTAACCTCTTAT
ATCCGXXXXXXXXXXTAGTATTACCACTGATCATATTTCACCAGCCGGTAACATCAATAG
AAAATCATCAGCCGCTCGTTATTTAGAGTCCAAAGGTGTAAAACCAGAAGATTTTAACAC
CTATGGATCTCGTAGAGGTAATGATGAAATTATGGTTAGAGGTACCTTTGCCAATACTCG
TATTGTTAATAAATTGGCTCCAGCCGTTGGTCCACAAACAACCTATGTACCAACTGGTGA
GTTAATGTTTATTTCAGATGCTGCAGAAAAATATCAATCAGAAGGTCATCAACTCATTGT
TTTGGCTGGTAGTGATTATGGCTCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGTCCATA
CCTTCAAGGAATTAAGTGTGTCATTGCCATCTCATTTGAACGTATTCATAGATCCAATCT
CGTTGGTATGGGTATTATCCCATTGCAATTCCAACCAGGTCAAAATGCCTCTACTTTGGG
TCTCACTGGTAAAGAACAATTCAATATCGAGTTACCAACTGATAAATCCCTCATTAAAAC
TGGTCAAACCGTAAAGGTTACCACTAATTGTGGTAAATCTTTTGAAACTATCCTTCGTTT
CGATACTCCAATCGAAGTTGAATATTGGGCAAATAATGGTATCTTATCTTATGTTTTAAG
AAAACTTTTACATTAATTTCTTTTTTTAATATAAAATAAAAAAAAATAATATTATTTAAA
A
sequence update 2002.10.25
Translated Amino Acid sequence 
*r*hk*si**SKGCIKIQDQTYNFYNLSKLQDPRIEKLPYSIRILLESAVRNCDNFEVHE
KDVENILNWENTANKVEIPFKPARVLLQDFTGVPAVVDLAAMRDAMKRLGGDPAKINPLV
PVDLVIDHSVQVDVSRTVDALEQNQKIEFHRNHERFSFLKWGAQAFDGLFIAPPGSGIVH
QVNLEYIAREVMNGTGNLLYP---

---SITTDHISPAGNINRKSSAARYLESKGVKPEDFNTYGSRRGNDEIMVRGTFANTRIV
NKLAPAVGPQTTYVPTGELMFISDAAEKYQSEGHQLIVLAGSDYGSGSSRDWAAKGPYLQ
GIKCVIAISFERIHRSNLVGMGIIPLQFQPGQNASTLGLTGKEQFNIELPTDKSLIKTGQ
TVKVTTNCGKSFETILRFDTPIEVEYWANNGILSYVLRKLLH*flfli*nkkk*yylk
Translated Amino Acid sequence (All Frames) 
Frame A:
lkddtnnpfdkvkdvlkskikliifiifqnfktqelknyhtpleffwnqqfvivitlkyt
kkmlkis*igktlqiklkflsnqqeyyykillvyqqllilqq*emp*kd*vviqqklthl
fqli*llitlfklmyqellml*skikrlnsieimkdfhf*ngvlkhlmdylllhlevvly
ik*i*silqekl*melvtsyi---

---*yyh*syftsr*hq*kiisrslfrvqrcktrrf*hlwis*r***nyg*rylcqysyc
**igssrwstnnlctnw*vnvyfrccrkisirrssthcfgw**lwlw*f*rlgcqrsips
rn*vchchli*tys*iqsrwygyypiaiptrskclyfgshw*rtiqyrvtn**iph*nws
nrkgyh*lw*if*nypsfrysnrs*ilgk*wylilcfkktftlisffnik*kkiilfk

Frame B:
lkmtqiihlik*rmy*nprsnl*fl*sfktsrpkn*kitilh*nsfgisss*l**l*str
krc*kylklgkhck*s*nsfqtsksiitrfywctssc*scsnerchekirw*sskn*ptc
ss*fsy*slcss*ciknc*cfrakskd*ip*ks*kififkmgcssi*wiiycstwkwyct
sskfrvyckrsyewnw*plis---

---SITTDHISPAGNINRKSSAARYLESKGVKPEDFNTYGSRRGNDEIMVRGTFANTRIV
NKLAPAVGPQTTYVPTGELMFISDAAEKYQSEGHQLIVLAGSDYGSGSSRDWAAKGPYLQ
GIKCVIAISFERIHRSNLVGMGIIPLQFQPGQNASTLGLTGKEQFNIELPTDKSLIKTGQ
TVKVTTNCGKSFETILRFDTPIEVEYWANNGILSYVLRKLLH*flfli*nkkk*yylk

Frame C:
*r*hk*si**SKGCIKIQDQTYNFYNLSKLQDPRIEKLPYSIRILLESAVRNCDNFEVHE
KDVENILNWENTANKVEIPFKPARVLLQDFTGVPAVVDLAAMRDAMKRLGGDPAKINPLV
PVDLVIDHSVQVDVSRTVDALEQNQKIEFHRNHERFSFLKWGAQAFDGLFIAPPGSGIVH
QVNLEYIAREVMNGTGNLLYP---

---vlpliifhqpvtsienhqplvi*spkv*nqkiltpmdlvevmmklwlevplpilvll
inwlqplvhkqpmyqlvs*clfqmlqkninqkvinslfwlvvimalvvleiglpkvhtfk
elsvslpshlnvfidpislvwvlshcnsnqvkmpllwvslvknnsissyqlinpslklvk
p*rlplivvnllklsfvsilqsklnigqimvsylmf*enfyinfff*ykikknnii*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ262 (VHQ262Q) /CSM/VH/VHQ2-C/VHQ262Q.Seq.d/                       2512   0.0
VHQ461 (VHQ461Q) /CSM/VH/VHQ4-C/VHQ461Q.Seq.d/                       1352   0.0
VHQ432 (VHQ432Q) /CSM/VH/VHQ4-B/VHQ432Q.Seq.d/                       1352   0.0
VHQ267 (VHQ267Q) /CSM/VH/VHQ2-C/VHQ267Q.Seq.d/                       1352   0.0
VHO844 (VHO844Q) /CSM/VH/VHO8-B/VHO844Q.Seq.d/                       1352   0.0
VHO678 (VHO678Q) /CSM/VH/VHO6-D/VHO678Q.Seq.d/                       1352   0.0
VHK111 (VHK111Q) /CSM/VH/VHK1-A/VHK111Q.Seq.d/                       1352   0.0
VHI493 (VHI493Q) /CSM/VH/VHI4-D/VHI493Q.Seq.d/                       1352   0.0
VHD137 (VHD137Q) /CSM/VH/VHD1-B/VHD137Q.Seq.d/                       1352   0.0
VHC406 (VHC406Q) /CSM/VH/VHC4-A/VHC406Q.Seq.d/                       1352   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ446724) Dictyostelium discoideum cDNA clone:ddv63k16, 3' ...  1318   0.0    2
(BJ446718) Dictyostelium discoideum cDNA clone:ddv63j16, 3' ...  1318   0.0    2
(BJ446695) Dictyostelium discoideum cDNA clone:ddv63e18, 3' ...  1318   0.0    2
(BJ446667) Dictyostelium discoideum cDNA clone:ddv63p08, 3' ...  1318   0.0    2
(BJ445866) Dictyostelium discoideum cDNA clone:ddv60k20, 3' ...  1318   0.0    2
(BJ445829) Dictyostelium discoideum cDNA clone:ddv60d20, 3' ...  1318   0.0    2
(BJ443383) Dictyostelium discoideum cDNA clone:ddv52b23, 3' ...  1318   0.0    2
(BJ442813) Dictyostelium discoideum cDNA clone:ddv51e03, 3' ...  1318   0.0    2
(BJ441788) Dictyostelium discoideum cDNA clone:ddv47j24, 3' ...  1318   0.0    2
(BJ439079) Dictyostelium discoideum cDNA clone:ddv39j09, 3' ...  1318   0.0    1
dna update 2008.12.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54X73) RecName: Full=Probable cytoplasmic aconitate hydratase;...   441   e-122
AB236788_1(AB236788|pid:none) Trifolium pratense RNA for putativ...   289   1e-76
AP008209_256(AP008209|pid:none) Oryza sativa (japonica cultivar-...   288   4e-76
BT060820_1(BT060820|pid:none) Zea mays full-length cDNA clone ZM...   286   2e-75
BT060665_1(BT060665|pid:none) Zea mays full-length cDNA clone ZM...   285   3e-75
(Q23500) RecName: Full=Probable cytoplasmic aconitate hydratase;...   285   3e-75
(P49608) RecName: Full=Aconitate hydratase, cytoplasmic;        ...   282   2e-74
DQ294230_1(DQ294230|pid:none) Glossina morsitans morsitans TC165...   281   4e-74
(Q9SIB9) RecName: Full=Aconitate hydratase 2, mitochondrial;    ...   281   5e-74
AY050485_1(AY050485|pid:none) Prunus avium putative aconitase mR...   280   1e-73
protein update 2009. 7.22
PSORT 

 psg: 0.73  gvh: 0.34  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: mitochondrial

>> prediction for VHQ262 is cyt
5' end seq. ID VHQ262F
5' end seq. 
>VHQ262F.Seq
CTTAAAGATGACACAAATAATCCATTTGATAAAGTAAAGGATGTATTAAAATCCAAGATC
AAACTTATAATTTTTATAATCTTTCAAAACTTCAAGACCCAAGAATTGAAAAATTACCAT
ACTCCATTAGAATTCTTTTGGAATCAGCAGTTCGTAATTGTGATAACTTTGAAGTACACG
AAAAAGATGTTGAAAATATCTTAAATTGGGAAAACACTGCAAATAAAGTTGAAATTCCTT
TCAAACCAGCAAGAGTATTATTACAAGATTTTACTGGTGTACCAGCAGTTGTTGATCTTG
CAGCAATGAGAGATGCCATGAAAAGATTAGGTGGTGATCCAGCAAAAATTAACCCACTTG
TTCCAGTTGATTTAGTTATTGATCACTCTGTTCAAGTTGATGTATCAAGAACTGTTGATG
CTTTAGAGCAAAATCAAAAGATTGAATTCCATAGAAATCATGAAAGATTTTCATTTTTAA
AATGGGGTGCTCAAGCATTTGATGGATTATTTATTGCTCCACCTGGAAGTGGTATTGTAC
ATCAAGTAAATTTAGAGTATATTGCAAGAGAAGTTATGAATGGAACTGGTAACCTCTTAT
ATCCGNNNNNNNNNN
Length of 5' end seq. 615
3' end seq. ID VHQ262Z
3' end seq. 
>VHQ262Z.Seq
NNNNNNNNNNTAGTATTACCACTGATCATATTTCACCAGCCGGTAACATCAATAGAAAAT
CATCAGCCGCTCGTTATTTAGAGTCCAAAGGTGTAAAACCAGAAGATTTTAACACCTATG
GATCTCGTAGAGGTAATGATGAAATTATGGTTAGAGGTACCTTTGCCAATACTCGTATTG
TTAATAAATTGGCTCCAGCCGTTGGTCCACAAACAACCTATGTACCAACTGGTGAGTTAA
TGTTTATTTCAGATGCTGCAGAAAAATATCAATCAGAAGGTCATCAACTCATTGTTTTGG
CTGGTAGTGATTATGGCTCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGTCCATACCTTC
AAGGAATTAAGTGTGTCATTGCCATCTCATTTGAACGTATTCATAGATCCAATCTCGTTG
GTATGGGTATTATCCCATTGCAATTCCAACCAGGTCAAAATGCCTCTACTTTGGGTCTCA
CTGGTAAAGAACAATTCAATATCGAGTTACCAACTGATAAATCCCTCATTAAAACTGGTC
AAACCGTAAAGGTTACCACTAATTGTGGTAAATCTTTTGAAACTATCCTTCGTTTCGATA
CTCCAATCGAAGTTGAATATTGGGCAAATAATGGTATCTTATCTTATGTTTTAAGAAAAC
TTTTACATTAATTTCTTTTTTTAATATAAAATAAAAAAAAATAATATTATTTAAAA
Length of 3' end seq. 716
Connected seq. ID VHQ262P
Connected seq. 
>VHQ262P.Seq
CTTAAAGATGACACAAATAATCCATTTGATAAAGTAAAGGATGTATTAAAATCCAAGATC
AAACTTATAATTTTTATAATCTTTCAAAACTTCAAGACCCAAGAATTGAAAAATTACCAT
ACTCCATTAGAATTCTTTTGGAATCAGCAGTTCGTAATTGTGATAACTTTGAAGTACACG
AAAAAGATGTTGAAAATATCTTAAATTGGGAAAACACTGCAAATAAAGTTGAAATTCCTT
TCAAACCAGCAAGAGTATTATTACAAGATTTTACTGGTGTACCAGCAGTTGTTGATCTTG
CAGCAATGAGAGATGCCATGAAAAGATTAGGTGGTGATCCAGCAAAAATTAACCCACTTG
TTCCAGTTGATTTAGTTATTGATCACTCTGTTCAAGTTGATGTATCAAGAACTGTTGATG
CTTTAGAGCAAAATCAAAAGATTGAATTCCATAGAAATCATGAAAGATTTTCATTTTTAA
AATGGGGTGCTCAAGCATTTGATGGATTATTTATTGCTCCACCTGGAAGTGGTATTGTAC
ATCAAGTAAATTTAGAGTATATTGCAAGAGAAGTTATGAATGGAACTGGTAACCTCTTAT
ATCCG----------TAGTATTACCACTGATCATATTTCACCAGCCGGTAACATCAATAG
AAAATCATCAGCCGCTCGTTATTTAGAGTCCAAAGGTGTAAAACCAGAAGATTTTAACAC
CTATGGATCTCGTAGAGGTAATGATGAAATTATGGTTAGAGGTACCTTTGCCAATACTCG
TATTGTTAATAAATTGGCTCCAGCCGTTGGTCCACAAACAACCTATGTACCAACTGGTGA
GTTAATGTTTATTTCAGATGCTGCAGAAAAATATCAATCAGAAGGTCATCAACTCATTGT
TTTGGCTGGTAGTGATTATGGCTCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGTCCATA
CCTTCAAGGAATTAAGTGTGTCATTGCCATCTCATTTGAACGTATTCATAGATCCAATCT
CGTTGGTATGGGTATTATCCCATTGCAATTCCAACCAGGTCAAAATGCCTCTACTTTGGG
TCTCACTGGTAAAGAACAATTCAATATCGAGTTACCAACTGATAAATCCCTCATTAAAAC
TGGTCAAACCGTAAAGGTTACCACTAATTGTGGTAAATCTTTTGAAACTATCCTTCGTTT
CGATACTCCAATCGAAGTTGAATATTGGGCAAATAATGGTATCTTATCTTATGTTTTAAG
AAAACTTTTACATTAATTTCTTTTTTTAATATAAAATAAAAAAAAATAATATTATTTAAA
A
Length of connected seq. 1311
Full length Seq ID -
Full length Seq. -
Length of full length seq. -