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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHQ253
Library VH
(Link to library)
Clone ID VHQ253
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHQ253P
(Link to Original site)
Representative DNA sequence 
>VHQ253 (VHQ253Q) /CSM/VH/VHQ2-C/VHQ253Q.Seq.d/
AATTACTCTTAAAAATAAATAAATAATAATAATAATAAATTTTAAAATGACTGATTTGAA
AATTACATTAGTTAATGAAGATGGGGAATCAACAATAAGTGGTAAAGGTCATCCATTACC
AGCTCCCCTAATATTTCCTCCAATATATTGTTTCTGTTTTATCCAATATAAAACTGAAGG
TAAACTTTGGGACAAAAATGATTTTCAAATAAAATCAGGTAAAATTGAATTCGGCGGAGA
AGAATATGATATTACTGAATCTAAAGGCACATGGAGTAAAGATGATGAAGAAAACCACAT
CAAAGTTAGTTTACATCTAATTGTACCACCAAAAAAAATTTTCCAAAAAAATTTTTAGAT
CTCAAAAACAGATACAATGAAAAACAAAGAAATAAAAAATTTGCATTAATCTTATTTTCA
TCATAANTAANAAAAAAAXXXXXXXXXXATTTAATTACTCCATTACCTGGTGTTACTTCA
ACTAAACCTGGTTCAGCAACTAAACCTTTCTTTGGTATAGAATTACAAGTTTTAGACTCA
AAAACTGGTGAACGTTTATATATTAATCCCGATATCAATGGTTGTAAAGAAATTTCTGGT
GTATTGGCAATCTCTAAACCTTGGCCAGGTATTGCTCGTTCAGTTTATCGTTCTCA
sequence update 2002.10.25
Translated Amino Acid sequence 
lllkink*****ilk*li*klh*lmkmgnqq*vvkvihyqlp*yflqyivsvlsniklkv
nfgtkmifk*nqvklnsaeknmillnlkahgvkmmkkttsklvyi*lyhqkkfskkifrs
qkqiq*ktkk*KICINLIFIIXXKK---

---LITPLPGVTSTKPGSATKPFFGIELQVLDSKTGERLYINPDINGCKEISGVLAISKP
WPGIARSVYRS
Translated Amino Acid sequence (All Frames) 
Frame A:
nys*k*innnnnkf*nd*fenyis**rwginnkw*rssitsspnissnilflfypi*n*r
*tlgqk*fsnkir*n*irrrri*yy*i*rhme*r**rkphqs*ftsncttkknfpkkfld
lknrynekqrnkkfalilfss*xxkk---

---i*llhylvllqlnlvqqlnlslv*nykf*tqklvnvyilipismvvkkflvywqsln
lgqvllvqfivl

Frame B:
itlknk*iiiiinfkmtdlkitlvnedgestisgkghplpaplifppiycfcfiqykteg
klwdkndfqiksgkiefggeeyditeskgtwskddeenhikvslhlivppkkifqknf*i
sktdtmknkeiknlh*syfhhx*xk---

---fnysitwcyfn*twfsn*tflwyritsfrlknw*tfiy*sryqwl*rnfwcignl*t
larycsfslsfs

Frame C:
lllkink*****ilk*li*klh*lmkmgnqq*vvkvihyqlp*yflqyivsvlsniklkv
nfgtkmifk*nqvklnsaeknmillnlkahgvkmmkkttsklvyi*lyhqkkfskkifrs
qkqiq*ktkk*KICINLIFIIXXKK---

---LITPLPGVTSTKPGSATKPFFGIELQVLDSKTGERLYINPDINGCKEISGVLAISKP
WPGIARSVYRS
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSH317 (VSH317Q) /CSM/VS/VSH3-A/VSH317Q.Seq.d/                        559   e-158
VSA227 (VSA227Q) /CSM/VS/VSA2-B/VSA227Q.Seq.d/                        559   e-158
VHQ253 (VHQ253Q) /CSM/VH/VHQ2-C/VHQ253Q.Seq.d/                        559   e-158
VFK101 (VFK101Q) /CSM/VF/VFK1-A/VFK101Q.Seq.d/                        559   e-158
SSM842 (SSM842Q) /CSM/SS/SSM8-B/SSM842Q.Seq.d/                        559   e-158
SSL312 (SSL312Q) /CSM/SS/SSL3-A/SSL312Q.Seq.d/                        559   e-158
SSK747 (SSK747Q) /CSM/SS/SSK7-B/SSK747Q.Seq.d/                        559   e-158
SSJ339 (SSJ339Q) /CSM/SS/SSJ3-B/SSJ339Q.Seq.d/                        559   e-158
SSI182 (SSI182Q) /CSM/SS/SSI1-D/SSI182Q.Seq.d/                        559   e-158
SSG857 (SSG857Q) /CSM/SS/SSG8-C/SSG857Q.Seq.d/                        559   e-158
own update 2004. 5.28
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ427787) Dictyostelium discoideum cDNA clone:ddv63i14, 5' ...   557   e-162  2
(AU266323) Dictyostelium discoideum vegetative cDNA clone:VS...   555   e-162  2
(C89796) Dictyostelium discoideum slug cDNA, clone SSG857.        557   e-162  2
(C90945) Dictyostelium discoideum slug cDNA, clone SSJ339.        557   e-162  2
(AU267249) Dictyostelium discoideum vegetative cDNA clone:VS...   557   e-162  2
(C93221) Dictyostelium discoideum slug cDNA, clone SSM842.        557   e-162  2
(BJ414955) Dictyostelium discoideum cDNA clone:ddv21a01, 5' ...   557   e-162  2
(C92629) Dictyostelium discoideum slug cDNA, clone SSF552.        557   e-162  2
(C93182) Dictyostelium discoideum slug cDNA, clone SSM748.        555   e-161  2
(AU261366) Dictyostelium discoideum vegetative cDNA clone:VS...   557   e-161  2
dna update 2008.12.25
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P16643) RecName: Full=Glycoprotein 24B;          Short=gp24B; A...   221   2e-56
(P16642) RecName: Full=Glycoprotein 24A;          Short=gp24A; A...   181   2e-44
AC115612_37(AC115612|pid:none) Dictyostelium discoideum chromoso...   140   2e-32
(Q54Z60) RecName: Full=Acetyl-coenzyme A synthetase;          EC...   140   2e-32
(Q558X5) RecName: Full=Contact site B protein C;  &AC117075_12(A...   122   9e-27
(Q556L8) RecName: Full=Uncharacterized protein csb family protei...    89   1e-16
CR940353_190(CR940353|pid:none) Theileria annulata strain Ankara...    77   3e-13
(Q54EJ7) RecName: Full=Uncharacterized protein csb family protei...    75   2e-12
(Q55B72) RecName: Full=Uncharacterized protein csb family protei...    74   4e-12
(Q55BG7) RecName: Full=Uncharacterized protein csb family protei...    71   3e-11
protein update 2009. 7.22
PSORT 

 psg: 0.92  gvh: 0.42  alm: 0.37  top: 0.60  tms: 0.00  mit: 0.33  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VHQ253 is cyt
5' end seq. ID VHQ253F
5' end seq. 
>VHQ253F.Seq
AATTACTCTTAAAAATAAATAAATAATAATAATAATAAATTTTAAAATGACTGATTTGAA
AATTACATTAGTTAATGAAGATGGGGAATCAACAATAAGTGGTAAAGGTCATCCATTACC
AGCTCCCCTAATATTTCCTCCAATATATTGTTTCTGTTTTATCCAATATAAAACTGAAGG
TAAACTTTGGGACAAAAATGATTTTCAAATAAAATCAGGTAAAATTGAATTCGGCGGAGA
AGAATATGATATTACTGAATCTAAAGGCACATGGAGTAAAGATGATGAAGAAAACCACAT
CAAAGTTAGTTTACATCTAATTGTACCACCAAAAAAAATTTTCCAAAAAAATTTTTAGAT
CTCAAAAACAGATACAATGAAAAACAAAGAAATAAAAAATTTGCATTAATCTTATTTTCA
TCATAANTAANAAAAAAANNNNNNNNNN
Length of 5' end seq. 448
3' end seq. ID VHQ253Z
3' end seq. 
>VHQ253Z.Seq
NNNNNNNNNNATTTAATTACTCCATTACCTGGTGTTACTTCAACTAAACCTGGTTCAGCA
ACTAAACCTTTCTTTGGTATAGAATTACAAGTTTTAGACTCAAAAACTGGTGAACGTTTA
TATATTAATCCCGATATCAATGGTTGTAAAGAAATTTCTGGTGTATTGGCAATCTCTAAA
CCTTGGCCAGGTATTGCTCGTTCAGTTTATCGTTCTCA
Length of 3' end seq. 218
Connected seq. ID VHQ253P
Connected seq. 
>VHQ253P.Seq
AATTACTCTTAAAAATAAATAAATAATAATAATAATAAATTTTAAAATGACTGATTTGAA
AATTACATTAGTTAATGAAGATGGGGAATCAACAATAAGTGGTAAAGGTCATCCATTACC
AGCTCCCCTAATATTTCCTCCAATATATTGTTTCTGTTTTATCCAATATAAAACTGAAGG
TAAACTTTGGGACAAAAATGATTTTCAAATAAAATCAGGTAAAATTGAATTCGGCGGAGA
AGAATATGATATTACTGAATCTAAAGGCACATGGAGTAAAGATGATGAAGAAAACCACAT
CAAAGTTAGTTTACATCTAATTGTACCACCAAAAAAAATTTTCCAAAAAAATTTTTAGAT
CTCAAAAACAGATACAATGAAAAACAAAGAAATAAAAAATTTGCATTAATCTTATTTTCA
TCATAANTAANAAAAAAA----------ATTTAATTACTCCATTACCTGGTGTTACTTCA
ACTAAACCTGGTTCAGCAACTAAACCTTTCTTTGGTATAGAATTACAAGTTTTAGACTCA
AAAACTGGTGAACGTTTATATATTAATCCCGATATCAATGGTTGTAAAGAAATTTCTGGT
GTATTGGCAATCTCTAAACCTTGGCCAGGTATTGCTCGTTCAGTTTATCGTTCTCA
Length of connected seq. 646
Full length Seq ID -
Full length Seq. -
Length of full length seq. -