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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHQ143
Library VH
(Link to library)
Clone ID VHQ143
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15722-1
Original site URL
Representative seq. ID VHQ143P
(Link to Original site)
Representative DNA sequence 
>VHQ143 (VHQ143Q) /CSM/VH/VHQ1-B/VHQ143Q.Seq.d/
AATTTAAAAAAAATAAAGGAAAAAACACACCAAAATTAAATAAGTAAGAATAAAAATGAA
ATATTTATTAGTATATTTACACATATTACTATTTTGTTTATTAAAGATCAATAGCCAAAA
GTATTGCACTTATAGTGGTGTTGGAGAAGATTATAATATCAAATTTGATGTCTTACAAAC
ATATGGTGTTGGTTTTCATTACTCATATTTAAGTGTTATTTTTACAACTTGTAATTATAC
TATTCCTTGTGGTAGTAATAGTAATAATGTATTGTGTGGATCATATTCAATTGTTCCACG
CAAGTATCTTTCATTGGGATTTGTTAATGGCAGTACACATAGTTATAGCAAAGACGGACT
AATGATAACATATATATCACAGGATACAACAAATGATAATGATTGCAAAAGATATACAAC
AAATGTGATTTTTCAATGTAGACCAGATGGTGATGTATCCGATTCATTAGATGTTGGCAT
CGACAATATACTTATTGATTCATGTATTGTTGAATTCACACTCTCTTCACGTACAGTGTG
TCAAACATGTCCAAACTGTTTAAAAACACATCAAACATCATGTAATGTGAAAAGTGGGTT
GGTGTACTTGTGATGGTXXXXXXXXXXATTGTTCGACNCCAAATGGTACTTGTGATAATA
ANACTGGTAATGGACATCCCATAATGAACATTTTGGTAATGGTTGTGAATTTACTCGATG
TCCACTCGATTGTTCGACACCAAATGGTACTTGTGATAATAATACTGGTAATTGTACATG
CCATAATGAACATTTTGGTAATTCTTGTGAATTTACTCGATGTCCACTCGATTGTTCGAC
ACCAAATGGTACTTGTGATAATAATACTGGTAATTGTACATGCCATAATGAACATTTTGG
TAATGGTTGTGAATTCACTCAATGCCCACTCTATTGTTCAACACCAAATGGTACTTGTGA
TATTAATAGTGGTATTTGTACATGTGATAATGAACATATTGGTAATGGTTGCGAAATTAA
ATTTATTGAATGTAAACATAAATGTTCAACCAAACATGGAATATGTGATAATGATAGTGG
TAATTGTAAATGTGATACTCAAACAAAAGGATTAACGTGTGAAGAATCAAGATTATTAAT
TGAATCATTAGACTCAATCAATTCAAAAGGTGGTACAATAAATATAATTGGTTATTTTGG
AAATACAACATCATTATTAACAATTAAAATTGGTGAATCACAATGTAAAAATATCAAAGT
ATTCAATGAAACAACATTAAAATGTGATATTGGTGAAGGTAAAGGTATTCATAATATTAC
AATCATTGATGATGACCTTTCGTTCACAGCAATCAATAAGTTCCAATATCTCGATGAAAA
AACAAAAATCTCAAAAATATCGAGAGGTGCAATTGCTGGTATTGTTGTTGGTTGTGTTGT
TGCTGTTTCATTAATTGGTTCTATTGCTTTTTATTATCATCACAAAAATAAAAAGGTT
sequence update 2002.10.25
Translated Amino Acid sequence 
nlkkikekthqn*isknkneifisifthitilfikdq*pkvlhl*wcwrrl*yqi*cltn
iwcwfsllifkcyfynl*lyyslw*****civwiifncstqvsfigic*wqyt*l*qrrt
ndniyitgynk***lqkiynkcdfsm*trw*cirfircwhrqyty*fmyc*ihtlftysv
snmsklfkntsnim*CEKWVGVLVM---

---CSTPNGTCDNXTGNGHPIMNILVMVVNLLDVHSIVRHQMVLVIIILVIVHAIMNILV
ILVNLLDVHSIVRHQMVLVIIILVIVHAIMNILVMVVNSLNAHSIVQHQMVLVILIVVFV
HVIMNILVMVAKLNLLNVNINVQPNMEYVIMIVVIVNVILKQKD*rvknqdy*lnh*tqs
iqkvvq*i*lvileiqhhy*qlklvnhnvkiskysmkqh*nvilvkvkvfiilqslmmtf
rsqqsissnismkkqksqkyrevqllvlllvvlllfh*lvlllfiiitkikr
Translated Amino Acid sequence (All Frames) 
Frame A:
nlkkikekthqn*isknkneifisifthitilfikdq*pkvlhl*wcwrrl*yqi*cltn
iwcwfsllifkcyfynl*lyyslw*****civwiifncstqvsfigic*wqyt*l*qrrt
ndniyitgynk***lqkiynkcdfsm*trw*cirfircwhrqyty*fmyc*ihtlftysv
snmsklfkntsnim*CEKWVGVLVM---

---ivrxqmvlviixlvmdip**tfw*wl*iysmstrlfdtkwyl***yw*lymp**tfw
*fl*iysmstrlfdtkwyl***yw*lymp**tfw*wl*ihsmptllfntkwyl*y**wyl
ym***tyw*wlrn*iy*m*t*mfnqtwnm****w*l*m*ysnkrinv*rikiin*iirln
qfkrwynkynwlfwkyniiinn*nw*itm*kyqsiq*nnikm*yw*r*rys*yynh***p
fvhsnq*vpisr*knknlkniercncwyccwlccccfinwfycfllssqk*kg

Frame B:
i*kk*rkkhtkik*vrikmkyllvylhillfcllkinsqkyctysgvgedynikfdvlqt
ygvgfhysylsvifttcnytipcgsnsnnvlcgsysivprkylslgfvngsthsyskdgl
mityisqdttndndckryttnvifqcrpdgdvsdsldvgidnilidsciveftlssrtvc
qtcpnclkthqtscnvksglvyl*w---

---lfdxkwyl***xw*wtshnehfgngceftrcpldcstpngtcdnntgnctchnehfg
nsceftrcpldcstpngtcdnntgnctchnehfgngceftqcplycstpngtcdinsgic
tcdnehigngceikfieckhkcstkhgicdndsgnckcdtqtkgltceesrlliesldsi
nskggtiniigyfgnttslltikigesqcknikvfnettlkcdigegkgihnitiidddl
sftainkfqyldektkiskisrgaiagivvgcvvavsligsiafyyhhknkkv

Frame C:
fkknkgkntpklnk*e*k*niy*yiytyyyfvy*rsiaksialivvlekiiisnlmsykh
mvlvfithi*vlflqlviilflvvvivimycvdhiqlfhasifhwdllmavhiviaktd*
**hiyhriqqmimiakdiqqm*ffnvdqmvmypih*mlastiyllihvllnshslhvqcv
khvqtv*khikhhvm*kvgwctcdg---

---CSTPNGTCDNXTGNGHPIMNILVMVVNLLDVHSIVRHQMVLVIIILVIVHAIMNILV
ILVNLLDVHSIVRHQMVLVIIILVIVHAIMNILVMVVNSLNAHSIVQHQMVLVILIVVFV
HVIMNILVMVAKLNLLNVNINVQPNMEYVIMIVVIVNVILKQKD*rvknqdy*lnh*tqs
iqkvvq*i*lvileiqhhy*qlklvnhnvkiskysmkqh*nvilvkvkvfiilqslmmtf
rsqqsissnismkkqksqkyrevqllvlllvvlllfh*lvlllfiiitkikr
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ143 (VHQ143Q) /CSM/VH/VHQ1-B/VHQ143Q.Seq.d/                       2535   0.0
VHH671 (VHH671Q) /CSM/VH/VHH6-C/VHH671Q.Seq.d/                       1433   0.0
VHP265 (VHP265Q) /CSM/VH/VHP2-C/VHP265Q.Seq.d/                       1431   0.0
VHI141 (VHI141Q) /CSM/VH/VHI1-B/VHI141Q.Seq.d/                       1427   0.0
VHA893 (VHA893Q) /CSM/VH/VHA8-D/VHA893Q.Seq.d/                       1423   0.0
VHO766 (VHO766Q) /CSM/VH/VHO7-C/VHO766Q.Seq.d/                       1413   0.0
VHK192 (VHK192Q) /CSM/VH/VHK1-D/VHK192Q.Seq.d/                       1407   0.0
VHM859 (VHM859Q) /CSM/VH/VHM8-C/VHM859Q.Seq.d/                       1390   0.0
VHA768 (VHA768Q) /CSM/VH/VHA7-C/VHA768Q.Seq.d/                       1390   0.0
VHN234 (VHN234Q) /CSM/VH/VHN2-B/VHN234Q.Seq.d/                       1388   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ446619) Dictyostelium discoideum cDNA clone:ddv63e11, 3' ...  1473   0.0    2
(AU284609) Dictyostelium discoideum gamete cDNA clone:FC-BE2...  1386   0.0    2
(BJ446052) Dictyostelium discoideum cDNA clone:ddv61a18, 3' ...  1386   0.0    3
(BJ441604) Dictyostelium discoideum cDNA clone:ddv47a11, 3' ...  1386   0.0    3
(BJ446789) Dictyostelium discoideum cDNA clone:ddv63h22, 3' ...  1380   0.0    2
(BJ443076) Dictyostelium discoideum cDNA clone:ddv51g23, 3' ...  1380   0.0    3
(BJ441429) Dictyostelium discoideum cDNA clone:ddv46m18, 3' ...  1380   0.0    3
(BJ441328) Dictyostelium discoideum cDNA clone:ddv46j09, 3' ...  1380   0.0    2
(BJ437001) Dictyostelium discoideum cDNA clone:ddv32j24, 3' ...  1380   0.0    3
(BJ444126) Dictyostelium discoideum cDNA clone:ddv55h07, 3' ...  1374   0.0    2
dna update 2008.12.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC117075_64(AC117075|pid:none) Dictyostelium discoideum chromoso...   125   3e-27
AC116100_9(AC116100|pid:none) Dictyostelium discoideum chromosom...   108   4e-22
AC116920_20(AC116920|pid:none) Dictyostelium discoideum chromoso...    97   2e-18
AC116967_7(AC116967|pid:none) Dictyostelium discoideum chromosom...    95   6e-18
AC116920_24(AC116920|pid:none) Dictyostelium discoideum chromoso...    94   2e-17
AC116963_42(AC116963|pid:none) Dictyostelium discoideum chromoso...    92   4e-17
AC116920_25(AC116920|pid:none) Dictyostelium discoideum chromoso...    90   2e-16
AC116967_8(AC116967|pid:none) Dictyostelium discoideum chromosom...    90   2e-16
AC117081_39(AC117081|pid:none) Dictyostelium discoideum chromoso...    89   5e-16
AC116956_34(AC116956|pid:none) Dictyostelium discoideum chromoso...    86   4e-15
protein update 2009. 7.22
PSORT 

 psg: 0.95  gvh: 0.61  alm: 0.12  top: 0.63  tms: 0.33  mit: 0.21  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 1.00  m_ : 0.00 

	 36.0 %: endoplasmic reticulum
	 24.0 %: plasma membrane
	 12.0 %: nuclear
	  8.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: cytoplasmic

>> prediction for VHQ143 is end
5' end seq. ID VHQ143F
5' end seq. 
>VHQ143F.Seq
AATTTAAAAAAAATAAAGGAAAAAACACACCAAAATTAAATAAGTAAGAATAAAAATGAA
ATATTTATTAGTATATTTACACATATTACTATTTTGTTTATTAAAGATCAATAGCCAAAA
GTATTGCACTTATAGTGGTGTTGGAGAAGATTATAATATCAAATTTGATGTCTTACAAAC
ATATGGTGTTGGTTTTCATTACTCATATTTAAGTGTTATTTTTACAACTTGTAATTATAC
TATTCCTTGTGGTAGTAATAGTAATAATGTATTGTGTGGATCATATTCAATTGTTCCACG
CAAGTATCTTTCATTGGGATTTGTTAATGGCAGTACACATAGTTATAGCAAAGACGGACT
AATGATAACATATATATCACAGGATACAACAAATGATAATGATTGCAAAAGATATACAAC
AAATGTGATTTTTCAATGTAGACCAGATGGTGATGTATCCGATTCATTAGATGTTGGCAT
CGACAATATACTTATTGATTCATGTATTGTTGAATTCACACTCTCTTCACGTACAGTGTG
TCAAACATGTCCAAACTGTTTAAAAACACATCAAACATCATGTAATGTGAAAAGTGGGTT
GGTGTACTTGTGATGGTNNNNNNNNNN
Length of 5' end seq. 627
3' end seq. ID VHQ143Z
3' end seq. 
>VHQ143Z.Seq
NNNNNNNNNNATTGTTCGACNCCAAATGGTACTTGTGATAATAANACTGGTAATGGACAT
CCCATAATGAACATTTTGGTAATGGTTGTGAATTTACTCGATGTCCACTCGATTGTTCGA
CACCAAATGGTACTTGTGATAATAATACTGGTAATTGTACATGCCATAATGAACATTTTG
GTAATTCTTGTGAATTTACTCGATGTCCACTCGATTGTTCGACACCAAATGGTACTTGTG
ATAATAATACTGGTAATTGTACATGCCATAATGAACATTTTGGTAATGGTTGTGAATTCA
CTCAATGCCCACTCTATTGTTCAACACCAAATGGTACTTGTGATATTAATAGTGGTATTT
GTACATGTGATAATGAACATATTGGTAATGGTTGCGAAATTAAATTTATTGAATGTAAAC
ATAAATGTTCAACCAAACATGGAATATGTGATAATGATAGTGGTAATTGTAAATGTGATA
CTCAAACAAAAGGATTAACGTGTGAAGAATCAAGATTATTAATTGAATCATTAGACTCAA
TCAATTCAAAAGGTGGTACAATAAATATAATTGGTTATTTTGGAAATACAACATCATTAT
TAACAATTAAAATTGGTGAATCACAATGTAAAAATATCAAAGTATTCAATGAAACAACAT
TAAAATGTGATATTGGTGAAGGTAAAGGTATTCATAATATTACAATCATTGATGATGACC
TTTCGTTCACAGCAATCAATAAGTTCCAATATCTCGATGAAAAAACAAAAATCTCAAAAA
TATCGAGAGGTGCAATTGCTGGTATTGTTGTTGGTTGTGTTGTTGCTGTTTCATTAATTG
GTTCTATTGCTTTTTATTATCATCACAAAAATAAAAAGGTT
Length of 3' end seq. 881
Connected seq. ID VHQ143P
Connected seq. 
>VHQ143P.Seq
AATTTAAAAAAAATAAAGGAAAAAACACACCAAAATTAAATAAGTAAGAATAAAAATGAA
ATATTTATTAGTATATTTACACATATTACTATTTTGTTTATTAAAGATCAATAGCCAAAA
GTATTGCACTTATAGTGGTGTTGGAGAAGATTATAATATCAAATTTGATGTCTTACAAAC
ATATGGTGTTGGTTTTCATTACTCATATTTAAGTGTTATTTTTACAACTTGTAATTATAC
TATTCCTTGTGGTAGTAATAGTAATAATGTATTGTGTGGATCATATTCAATTGTTCCACG
CAAGTATCTTTCATTGGGATTTGTTAATGGCAGTACACATAGTTATAGCAAAGACGGACT
AATGATAACATATATATCACAGGATACAACAAATGATAATGATTGCAAAAGATATACAAC
AAATGTGATTTTTCAATGTAGACCAGATGGTGATGTATCCGATTCATTAGATGTTGGCAT
CGACAATATACTTATTGATTCATGTATTGTTGAATTCACACTCTCTTCACGTACAGTGTG
TCAAACATGTCCAAACTGTTTAAAAACACATCAAACATCATGTAATGTGAAAAGTGGGTT
GGTGTACTTGTGATGGT----------ATTGTTCGACNCCAAATGGTACTTGTGATAATA
ANACTGGTAATGGACATCCCATAATGAACATTTTGGTAATGGTTGTGAATTTACTCGATG
TCCACTCGATTGTTCGACACCAAATGGTACTTGTGATAATAATACTGGTAATTGTACATG
CCATAATGAACATTTTGGTAATTCTTGTGAATTTACTCGATGTCCACTCGATTGTTCGAC
ACCAAATGGTACTTGTGATAATAATACTGGTAATTGTACATGCCATAATGAACATTTTGG
TAATGGTTGTGAATTCACTCAATGCCCACTCTATTGTTCAACACCAAATGGTACTTGTGA
TATTAATAGTGGTATTTGTACATGTGATAATGAACATATTGGTAATGGTTGCGAAATTAA
ATTTATTGAATGTAAACATAAATGTTCAACCAAACATGGAATATGTGATAATGATAGTGG
TAATTGTAAATGTGATACTCAAACAAAAGGATTAACGTGTGAAGAATCAAGATTATTAAT
TGAATCATTAGACTCAATCAATTCAAAAGGTGGTACAATAAATATAATTGGTTATTTTGG
AAATACAACATCATTATTAACAATTAAAATTGGTGAATCACAATGTAAAAATATCAAAGT
ATTCAATGAAACAACATTAAAATGTGATATTGGTGAAGGTAAAGGTATTCATAATATTAC
AATCATTGATGATGACCTTTCGTTCACAGCAATCAATAAGTTCCAATATCTCGATGAAAA
AACAAAAATCTCAAAAATATCGAGAGGTGCAATTGCTGGTATTGTTGTTGGTTGTGTTGT
TGCTGTTTCATTAATTGGTTCTATTGCTTTTTATTATCATCACAAAAATAAAAAGGTT
Length of connected seq. 1488
Full length Seq ID -
Full length Seq. -
Length of full length seq. -