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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHQ103
Library VH
(Link to library)
Clone ID VHQ103
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15748-1
Original site URL
Representative seq. ID VHQ103P
(Link to Original site)
Representative DNA sequence 
>VHQ103 (VHQ103Q) /CSM/VH/VHQ1-A/VHQ103Q.Seq.d/
AACAACAACAATACATTTTACATATATATTTAGAATATTCAAAATTTCTTATCGATACCC
TTTATATATATACAGTTTTACAATATTGTTGTAAAATTAAGTCAGATACAAACAAGAATG
AATATAAATCAAATTATGTCTCTTTTTGTTTGTTTTTGTTTATTTTCGTCAATATACTTA
ATAAATACTGTTCATTCACAAGGACCTTTCCCATATAATACTACTGAGGGTTGTTCGATG
AATGGTTTAAATGCAAGTTATATTGAAAGTGGTCTTTATCCACCTTATCATAATCAAATT
TTAGCTCCAGGTACATGCCAATATTCACATCCAGAATATTCAGAAACATCATGTTGTACA
AATGGTCAAACACTTGTTTTATTATCAAACATGGTAATGGGATCTGCAGTATTTGCACGT
TGTCCAGCATGTTTATTTAATGTATGGGATTTGTGGTGTGCATCATCATGTTCACCATAT
CAACAATCATTTATGGTACCAACAAGTATCGATAGTAAAACAGGACAAATCTTAAGCGTT
GATTTCGTCATTCATCCCGATTACGCTTGGGGTTTATATAATTCATGTAGTGGTGTAAAT
TCXXXXXXXXXXTCATGTACCATATGATACTTGTAAAGGCTGTATGTCATGTAATCCATC
AAAATGGTGATTTGAAGCTCATAACGAGGTTTGTAATGATTTTAATGCATGTACTGAAAA
TGTCTGTTCTCCAGTTAAAGTTGGTGCAGGTGTTGGTGAATGTGTAACTACCAATACAAC
ATGTGCACCAACCACAAACGTTGATAAATGTAAAAACTACCTTTNCGCAAGAGTGTAGCA
ATAATGGATATCCTTTTGGCATTGGGTGGTGTATTTATTGTTTGTCTCATTTTACTATTG
AATCCATTGGTTTCTTTCTTGGTTGTAATTTCCGTTGGTATGATTTGTGTCGATTTATTA
GGTATCATGGCATTATGGAACATCTCTTTGAATGCAGTTTCCGTTGTAAACGTTGTTATG
GCCATTGGTATTGGTATCGAGTTTTGTGTTCACATTGCTTCAACCTTTATCAATGCTCCA
AAACATTTCTCAAGAGATCAAAAAGCAAAATATGCCGTCACTGAGATGGGTAGTTCCATC
ATTAGTGGTATCTTTATCACAAAATTATTGGGTGTCGTGGTTTTAGGTTTCTCAACTTCA
GAAATTTTCACTGTTTATTACTTTAGAATGTATCTCAGTATAGTTTTCTTGGGTGGTTTA
CATGGTTTAGTTTTCTTACCAGTATTATTATCTTTATTTGGAACTGATACCCTTCAATTA
GATCGTCTTTGTAAATCTAAAAAAAAAGTTATTCACTTAA
sequence update 2002.10.25
Translated Amino Acid sequence 
nnnntfyiyi*NIQNFLSIPFIYIQFYNIVVKLSQIQTRMNINQIMSLFVCFCLFSSIYL
INTVHSQGPFPYNTTEGCSMNGLNASYIESGLYPPYHNQILAPGTCQYSHPEYSETSCCT
NGQTLVLLSNMVMGSAVFARCPACLFNVWDLWCASSCSPYQQSFMVPTSIDSKTGQILSV
DFVIHPDYAWGLYNSCSGVN---

---MYHMILVKAVCHVIHQNGDLKLITRFVMILMHVLKMSVLQLKLVQVLVNV*lpiqhv
hqpqtlinvkttfxqecsnngypfgigwciyclshftiesigfflgcnfrwydlcrfiry
hgimehlfecsfrckrcyghwywyrvlcshcfnlyqcsktflkrskskicrh*dg*fhh*
wylyhkiigcrgfrflnfrnfhclll*nvsqysflgwftwfsfltsiiifiwn*ypsirs
sl*i*kksysl
Translated Amino Acid sequence (All Frames) 
Frame A:
nnnntfyiyi*NIQNFLSIPFIYIQFYNIVVKLSQIQTRMNINQIMSLFVCFCLFSSIYL
INTVHSQGPFPYNTTEGCSMNGLNASYIESGLYPPYHNQILAPGTCQYSHPEYSETSCCT
NGQTLVLLSNMVMGSAVFARCPACLFNVWDLWCASSCSPYQQSFMVPTSIDSKTGQILSV
DFVIHPDYAWGLYNSCSGVN---

---scti*yl*rlyvm*sikmvi*ss*rgl**f*cmy*kclfss*swcrcw*mcnyqynm
ctnhkr**m*klpxrksvaimdillalggvfivclilllnplvsflvvisvgmicvdllg
imalwnislnavsvvnvvmaigigiefcvhiastfinapkhfsrdqkakyavtemgssii
sgifitkllgvvvlgfstseiftvyyfrmylsivflgglhglvflpvllslfgtdtlqld
rlckskkkvihl

Frame B:
tttihftyifrifkisyryplyiysftill*n*vrykqe*i*iklclflfvfvyfrqyt*
*ilfihkdlshiillrvvr*mv*mqvilkvvfihliiikf*lqvhanihiqniqkhhvvq
mvkhlfyyqtw*wdlqylhvvqhvylmygicgvhhhvhhinnhlwyqqvsivkqdks*al
issfipitlgvyiihvvv*i---

---hvpydtckgcmscnpskw*feahnevcndfnactenvcspvkvgagvgecvttnttc
apttnvdkcknylxarv*q*wisfwhwvvyllfvsfyy*ihwflswl*fplv*fvsiy*v
swhygtsl*mqfpl*tllwplvlvssfvftllqplsmlqnisqeikkqnmpslrwvvpsl
vvslsqnywvswf*vsqlqkfslfitlecisv*fswvvymv*fsyqyyylylelipfn*i
vfvnlkkklft*

Frame C:
qqqyilhiyleyskflidtlyiytvlqycckiksdtnkneyksnyvsfclflfifvniln
kycsftrtfpi*yy*glfdewfkckly*kwslstls*snfssrympiftsrifrnimlyk
wsntcfiikhgngicsictlssmfi*cmgfvvciimftistiiygtnkyr**nrtnlkr*
frhssrlrlgfi*fm*wckf---

---MYHMILVKAVCHVIHQNGDLKLITRFVMILMHVLKMSVLQLKLVQVLVNV*lpiqhv
hqpqtlinvkttfxqecsnngypfgigwciyclshftiesigfflgcnfrwydlcrfiry
hgimehlfecsfrckrcyghwywyrvlcshcfnlyqcsktflkrskskicrh*dg*fhh*
wylyhkiigcrgfrflnfrnfhclll*nvsqysflgwftwfsfltsiiifiwn*ypsirs
sl*i*kksysl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ103 (VHQ103Q) /CSM/VH/VHQ1-A/VHQ103Q.Seq.d/                       2258   0.0
VHI844 (VHI844Q) /CSM/VH/VHI8-B/VHI844Q.Seq.d/                       1013   0.0
SHJ888 (SHJ888Q) /CSM/SH/SHJ8-D/SHJ888Q.Seq.d/                       1013   0.0
SHG768 (SHG768Q) /CSM/SH/SHG7-C/SHG768Q.Seq.d/                       1013   0.0
SHG328 (SHG328Q) /CSM/SH/SHG3-B/SHG328Q.Seq.d/                       1013   0.0
SHD228 (SHD228Q) /CSM/SH/SHD2-B/SHD228Q.Seq.d/                       1013   0.0
CHH472 (CHH472Q) /CSM/CH/CHH4-C/CHH472Q.Seq.d/                       1013   0.0
CHH334 (CHH334Q) /CSM/CH/CHH3-B/CHH334Q.Seq.d/                       1013   0.0
AHQ411 (AHQ411Q) /CSM/AH/AHQ4-A/AHQ411Q.Seq.d/                       1013   0.0
AHO583 (AHO583Q) /CSM/AH/AHO5-D/AHO583Q.Seq.d/                       1013   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ446541) Dictyostelium discoideum cDNA clone:ddv63e01, 3' ...  1433   0.0    1
(AF202726) Dictyostelium discoideum NPC1 protein gene, compl...  1013   0.0    5
(AF202725) Dictyostelium discoideum NPC1 protein mRNA, compl...  1013   0.0    4
(BJ427627) Dictyostelium discoideum cDNA clone:ddv63e01, 5' ...   864   0.0    2
(BJ423313) Dictyostelium discoideum cDNA clone:ddv48d20, 5' ...   864   0.0    2
(BJ420976) Dictyostelium discoideum cDNA clone:ddv41e08, 5' ...   864   0.0    2
(BJ393973) Dictyostelium discoideum cDNA clone:dds33h10, 5' ...   864   0.0    2
(BJ393633) Dictyostelium discoideum cDNA clone:dds32e04, 5' ...   864   0.0    2
(BJ393157) Dictyostelium discoideum cDNA clone:dds30g08, 5' ...   852   0.0    2
(BJ420357) Dictyostelium discoideum cDNA clone:ddv39e02, 5' ...   848   0.0    2
dna update 2008.12.23
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF202725_1(AF202725|pid:none) Dictyostelium discoideum NPC1 prot...   350   9e-95
AC025815_1(AC025815|pid:none) Arabidopsis thaliana chromosome 1 ...   132   4e-29
AL606694_11(AL606694|pid:none) Oryza sativa genomic DNA, chromos...   129   2e-28
AF503634_1(AF503634|pid:none) Felis catus Niemann-Pick C1 mRNA, ...   125   4e-27
AF258783_1(AF258783|pid:none) Felis catus Niemann-Pick type C1 d...   125   4e-27
AF182744_1(AF182744|pid:none) Cricetulus griseus Niemann-Pick ty...   125   5e-27
BC151276_1(BC151276|pid:none) Bos taurus Niemann-Pick disease, t...   122   3e-26
AF299073_1(AF299073|pid:none) Bos taurus Niemann-Pick type C1 di...   122   3e-26
(P56941) RecName: Full=Niemann-Pick C1 protein; Flags: Precursor...   121   5e-26
AB209048_1(AB209048|pid:none) Homo sapiens mRNA for Niemann-Pick...   120   9e-26
protein update 2009. 7.21
PSORT 

 psg: 0.92  gvh: 0.64  alm: 0.23  top: 0.37  tms: 0.07  mit: 0.40  mip: 0.06
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.29  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: endoplasmic reticulum
	 20.0 %: cytoplasmic
	 20.0 %: Golgi
	 16.0 %: mitochondrial
	 12.0 %: nuclear
	  4.0 %: plasma membrane
	  4.0 %: extracellular, including cell wall

>> prediction for VHQ103 is end
5' end seq. ID VHQ103F
5' end seq. 
>VHQ103F.Seq
AACAACAACAATACATTTTACATATATATTTAGAATATTCAAAATTTCTTATCGATACCC
TTTATATATATACAGTTTTACAATATTGTTGTAAAATTAAGTCAGATACAAACAAGAATG
AATATAAATCAAATTATGTCTCTTTTTGTTTGTTTTTGTTTATTTTCGTCAATATACTTA
ATAAATACTGTTCATTCACAAGGACCTTTCCCATATAATACTACTGAGGGTTGTTCGATG
AATGGTTTAAATGCAAGTTATATTGAAAGTGGTCTTTATCCACCTTATCATAATCAAATT
TTAGCTCCAGGTACATGCCAATATTCACATCCAGAATATTCAGAAACATCATGTTGTACA
AATGGTCAAACACTTGTTTTATTATCAAACATGGTAATGGGATCTGCAGTATTTGCACGT
TGTCCAGCATGTTTATTTAATGTATGGGATTTGTGGTGTGCATCATCATGTTCACCATAT
CAACAATCATTTATGGTACCAACAAGTATCGATAGTAAAACAGGACAAATCTTAAGCGTT
GATTTCGTCATTCATCCCGATTACGCTTGGGGTTTATATAATTCATGTAGTGGTGTAAAT
TCNNNNNNNNNN
Length of 5' end seq. 612
3' end seq. ID VHQ103Z
3' end seq. 
>VHQ103Z.Seq
NNNNNNNNNNTCATGTACCATATGATACTTGTAAAGGCTGTATGTCATGTAATCCATCAA
AATGGTGATTTGAAGCTCATAACGAGGTTTGTAATGATTTTAATGCATGTACTGAAAATG
TCTGTTCTCCAGTTAAAGTTGGTGCAGGTGTTGGTGAATGTGTAACTACCAATACAACAT
GTGCACCAACCACAAACGTTGATAAATGTAAAAACTACCTTTNCGCAAGAGTGTAGCAAT
AATGGATATCCTTTTGGCATTGGGTGGTGTATTTATTGTTTGTCTCATTTTACTATTGAA
TCCATTGGTTTCTTTCTTGGTTGTAATTTCCGTTGGTATGATTTGTGTCGATTTATTAGG
TATCATGGCATTATGGAACATCTCTTTGAATGCAGTTTCCGTTGTAAACGTTGTTATGGC
CATTGGTATTGGTATCGAGTTTTGTGTTCACATTGCTTCAACCTTTATCAATGCTCCAAA
ACATTTCTCAAGAGATCAAAAAGCAAAATATGCCGTCACTGAGATGGGTAGTTCCATCAT
TAGTGGTATCTTTATCACAAAATTATTGGGTGTCGTGGTTTTAGGTTTCTCAACTTCAGA
AATTTTCACTGTTTATTACTTTAGAATGTATCTCAGTATAGTTTTCTTGGGTGGTTTACA
TGGTTTAGTTTTCTTACCAGTATTATTATCTTTATTTGGAACTGATACCCTTCAATTAGA
TCGTCTTTGTAAATCTAAAAAAAAAGTTATTCACTTAA
Length of 3' end seq. 758
Connected seq. ID VHQ103P
Connected seq. 
>VHQ103P.Seq
AACAACAACAATACATTTTACATATATATTTAGAATATTCAAAATTTCTTATCGATACCC
TTTATATATATACAGTTTTACAATATTGTTGTAAAATTAAGTCAGATACAAACAAGAATG
AATATAAATCAAATTATGTCTCTTTTTGTTTGTTTTTGTTTATTTTCGTCAATATACTTA
ATAAATACTGTTCATTCACAAGGACCTTTCCCATATAATACTACTGAGGGTTGTTCGATG
AATGGTTTAAATGCAAGTTATATTGAAAGTGGTCTTTATCCACCTTATCATAATCAAATT
TTAGCTCCAGGTACATGCCAATATTCACATCCAGAATATTCAGAAACATCATGTTGTACA
AATGGTCAAACACTTGTTTTATTATCAAACATGGTAATGGGATCTGCAGTATTTGCACGT
TGTCCAGCATGTTTATTTAATGTATGGGATTTGTGGTGTGCATCATCATGTTCACCATAT
CAACAATCATTTATGGTACCAACAAGTATCGATAGTAAAACAGGACAAATCTTAAGCGTT
GATTTCGTCATTCATCCCGATTACGCTTGGGGTTTATATAATTCATGTAGTGGTGTAAAT
TC----------TCATGTACCATATGATACTTGTAAAGGCTGTATGTCATGTAATCCATC
AAAATGGTGATTTGAAGCTCATAACGAGGTTTGTAATGATTTTAATGCATGTACTGAAAA
TGTCTGTTCTCCAGTTAAAGTTGGTGCAGGTGTTGGTGAATGTGTAACTACCAATACAAC
ATGTGCACCAACCACAAACGTTGATAAATGTAAAAACTACCTTTNCGCAAGAGTGTAGCA
ATAATGGATATCCTTTTGGCATTGGGTGGTGTATTTATTGTTTGTCTCATTTTACTATTG
AATCCATTGGTTTCTTTCTTGGTTGTAATTTCCGTTGGTATGATTTGTGTCGATTTATTA
GGTATCATGGCATTATGGAACATCTCTTTGAATGCAGTTTCCGTTGTAAACGTTGTTATG
GCCATTGGTATTGGTATCGAGTTTTGTGTTCACATTGCTTCAACCTTTATCAATGCTCCA
AAACATTTCTCAAGAGATCAAAAAGCAAAATATGCCGTCACTGAGATGGGTAGTTCCATC
ATTAGTGGTATCTTTATCACAAAATTATTGGGTGTCGTGGTTTTAGGTTTCTCAACTTCA
GAAATTTTCACTGTTTATTACTTTAGAATGTATCTCAGTATAGTTTTCTTGGGTGGTTTA
CATGGTTTAGTTTTCTTACCAGTATTATTATCTTTATTTGGAACTGATACCCTTCAATTA
GATCGTCTTTGTAAATCTAAAAAAAAAGTTATTCACTTAA
Length of connected seq. 1350
Full length Seq ID -
Full length Seq. -
Length of full length seq. -