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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHP874
Library VH
(Link to library)
Clone ID VHP874
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHP874P
(Link to Original site)
Representative DNA sequence 
>VHP874 (VHP874Q) /CSM/VH/VHP8-D/VHP874Q.Seq.d/
AACTCTCGAGTGCAAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATT
CGTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGAT
CACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAA
GTTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACC
ATTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCT
GGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTC
AGTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGC
GTCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATC
AAACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACT
GAAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAAC
ACTGAAGXXXXXXXXXXCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAG
GTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCA
AAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCA
CCCTCCACACTGATGCCATCCACANAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTG
TACTCTATGCTGCTGAATTAACTGCCTCCCCAACTNTNTTAGAACCAATNTACTTAGTAG
AAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTANAG
GTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATT
TACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAG
CTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGA
AAGCCACTGAAGTCGCTTTAGCCACCAGAAANAGAAAAGGTTTAGCCCCAGAAATCCCAG
ATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTTGAGTTTTCAAAA
ATAGTAATCAAATCTAAAAAAAATTTTAAAAAAAAAA
sequence update 2009. 3.30
Translated Amino Acid sequence 
NSRVQKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADK
VSGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDF
SSEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNT
EEAYLSFRRAIESVNVIVGNTE---

---PEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLH
TDAIHXGGGQIIPTARRVLYAAELTASPTXLEPXYLVEITAPENAIGGIYSVLNRRXGIV
IGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKAT
EVALATRXRKGLAPEIPDLDKFHEKL*ti*val*vfknsnqi*kkf*kk
Translated Amino Acid sequence (All Frames) 
Frame A:
NSRVQKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADK
VSGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDF
SSEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNT
EEAYLSFRRAIESVNVIVGNTE---

---PEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLH
TDAIHXGGGQIIPTARRVLYAAELTASPTXLEPXYLVEITAPENAIGGIYSVLNRRXGIV
IGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKAT
EVALATRXRKGLAPEIPDLDKFHEKL*ti*val*vfknsnqi*kkf*kk

Frame B:
tleckkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlk---

---qkvtvpisssmspkvfni*mklkilslvlsngppkkvlfvmkt*evsvsifmmspst
lmpstxvvvkssqllvvysmlln*lppqlx*nqxt**kllpqktllvvftlysivvxvll
lvknvvsvphyslskliyqy*npsvspliyvptlpvklshnvssiiglqlvlstkirkpl
ksl*ppexekv*pqksqiltnstknskqfkshfefskivikskknfkkk

Frame C:
lssakkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*---

---rr*rcqsprqchqrcsife*n*rffrwcfpmghqrrccl**kherypfqsl*chpph
*chpxrwwsnhpncssctlcc*inclpnxxrtnxlsrnycprkrywwyllctqssxrycy
w*rtsyrfpiilcqssftsirilrfhr*ftfphcrssfptmclrslgfnwccqqr*esh*
srfshqkxkrfsprnprs*qiprktlnnlsrtlsfqk**snlkkilkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/            2391   0.0
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                       1269   0.0
VHQ409 (VHQ409Q) /CSM/VH/VHQ4-A/VHQ409Q.Seq.d/                       1269   0.0
VHP832 (VHP832Q) /CSM/VH/VHP8-B/VHP832Q.Seq.d/                       1269   0.0
VHP765 (VHP765Q) /CSM/VH/VHP7-C/VHP765Q.Seq.d/                       1269   0.0
VHP589 (VHP589Q) /CSM/VH/VHP5-D/VHP589Q.Seq.d/                       1269   0.0
VHP213 (VHP213Q) /CSM/VH/VHP2-A/VHP213Q.Seq.d/                       1269   0.0
VHO796 (VHO796Q) /CSM/VH/VHO7-D/VHO796Q.Seq.d/                       1269   0.0
VHO710 (VHO710Q) /CSM/VH/VHO7-A/VHO710Q.Seq.d/                       1269   0.0
own update 2009. 4. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ446528) Dictyostelium discoideum cDNA clone:ddv63b04, 3' ...  1269   0.0    1
(BJ446440) Dictyostelium discoideum cDNA clone:ddv62a23, 3' ...  1269   0.0    1
(BJ446369) Dictyostelium discoideum cDNA clone:ddv62b17, 3' ...  1269   0.0    1
(BJ446358) Dictyostelium discoideum cDNA clone:ddv62p08, 3' ...  1269   0.0    1
(BJ446316) Dictyostelium discoideum cDNA clone:ddv62h12, 3' ...  1269   0.0    1
(BJ445933) Dictyostelium discoideum cDNA clone:ddv61i04, 3' ...  1269   0.0    1
(BJ445894) Dictyostelium discoideum cDNA clone:ddv60p23, 3' ...  1269   0.0    1
(BJ445876) Dictyostelium discoideum cDNA clone:ddv60m22, 3' ...  1269   0.0    1
(BJ445765) Dictyostelium discoideum cDNA clone:ddv60g14, 3' ...  1269   0.0    1
(BJ445607) Dictyostelium discoideum cDNA clone:ddv60d03, 3' ...  1269   0.0    1
dna update 2009. 4.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   263   7e-69
AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   259   2e-67
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   258   2e-67
DQ848932_1(DQ848932|pid:none) Scophthalmus maximus clone kbc101 ...   257   5e-67
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   257   5e-67
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   256   9e-67
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   256   1e-66
AY026074_1(AY026074|pid:none) Monosiga brevicollis elongation fa...   256   1e-66
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   256   2e-66
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   256   2e-66
protein update 2009. 4. 6
PSORT 

 psg: 0.00  gvh: 0.00  alm: 0.00  top: 0.00  tms: 0.00  mit: 0.00  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	100.0 %: 

>> prediction for VHP874 is

5' end seq. ID VHP874F
5' end seq. 
>VHP874F.Seq
AACTCTCGAGTGCAAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATT
CGTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGAT
CACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAA
GTTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACC
ATTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCT
GGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTC
AGTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGC
GTCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATC
AAACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACT
GAAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAAC
ACTGAAGXXXXXXXXXX
Length of 5' end seq. 617
3' end seq. ID VHP874Z
3' end seq. 
>VHP874Z.Seq
XXXXXXXXXXCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCA
ATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGG
TGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCA
CACTGATGCCATCCACANAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTA
TGCTGCTGAATTAACTGCCTCCCCAACTNTNTTAGAACCAATNTACTTAGTAGAAATTAC
TGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTANAGGTATTGT
TATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGT
ATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCC
ACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCAC
TGAAGTCGCTTTAGCCACCAGAAANAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGA
CAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTTGAGTTTTCAAAAATAGTAA
TCAAATCTAAAAAAAATTTTAAAAAAAAAA
Length of 3' end seq. 690
Connected seq. ID VHP874P
Connected seq. 
>VHP874P.Seq
AACTCTCGAGTGCAAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATT
CGTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGAT
CACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAA
GTTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACC
ATTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCT
GGTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTC
AGTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGC
GTCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATC
AAACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACT
GAAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAAC
ACTGAAG----------CCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAG
GTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCA
AAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCA
CCCTCCACACTGATGCCATCCACANAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTG
TACTCTATGCTGCTGAATTAACTGCCTCCCCAACTNTNTTAGAACCAATNTACTTAGTAG
AAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTANAG
GTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATT
TACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAG
CTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGA
AAGCCACTGAAGTCGCTTTAGCCACCAGAAANAGAAAAGGTTTAGCCCCAGAAATCCCAG
ATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTTGAGTTTTCAAAA
ATAGTAATCAAATCTAAAAAAAATTTTAAAAAAAAAA
Length of connected seq. 1287
Full length Seq ID -
Full length Seq. -
Length of full length seq. -